• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.378-386
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• 2009

A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected based on their high positive significant effect on the protease activity. A near-optimum medium formulation was then obtained that increased the protease yield by more than 5-fold. Thereafter, the response surface methodology(RSM) was adopted to acquire the best process conditions among the selected variables, where a 3-level Box-Behnken design was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the protease activity. The optimal combination of the major medium constituents for alkaline protease production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows: pH of 9.5, 2% peptone, and incubation time of 60 h. The predicted optimum alkaline protease activity was 3,213 U/ml/min, which was 6.4 times the activity with the basal medium.

• International Journal of Reliability and Applications
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• v.15 no.2
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• pp.85-98
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• 2014

Accelerated life testing (ALT) is a well famous technique in life testing and reliability studies, this is particularly used to induce so high stress leading to failure of the highly reliable units quickly under stipulated duration of time. The step-stress ALT is one of the systematic experimental strategy of ALT applied to fail the units in steps. In this article we focus on two important issues (i) necessity of life tests at higher steps with relevant causes (ii) to develop a new optimum test plan for 3-step SSALT under the modified cumulative exposure model proposed by Khamis and Higgins (1998). It is assumed that the lifetime of test units follows Rayleigh distribution and its scale parameter at constant stress level is assumed to be a log-linear function of the stress. The maximum likelihood estimates of the parameters involved in the step-stress ALT model are obtained. A simulation study is performed for numerical investigation of the proposed new optimum plan 3-step, step-stress ALT. The necessity of the life test units at 3-step step-stress is also numerically examined in comparison to simple step-stress setup.

• Structural Engineering and Mechanics
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• v.26 no.4
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• pp.459-477
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• 2007

A bi-directional tuned mass damper (BTMD) in which a mass connected by two translational springs and two viscous dampers in two orthogonal directions has been introduced to control coupled lateral and torsional vibrations of asymmetric building. An efficient control strategy has been presented in this context to control displacements as well as acceleration responses of asymmetric buildings having asymmetry in both plan and elevation. The building is idealized as a simplified 3D model with two translational and a rotational degrees of freedom for each floor. The principles of rigid body transformation have been incorporated to account for eccentricity between center of mass and center of rigidity. The effective and robust design of BTMD for controlling the vibrations in structures has been presented. The redundancy of optimum design has been checked. Non dominated sorting genetic algorithm (NSGA) has been used for tuning optimum stages and locations of BTMDs and its parameters for control of vibration of seismically excited buildings. The optimal locations have been observed to be reasonably compact and practically implementable.

• Journal of the Earthquake Engineering Society of Korea
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• v.6 no.5
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• pp.29-36
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• 2002

In the maintenance and retrofit planning of a bridge system, the optimal strategy for inspection and repair are suggested by minimizing the expected total life-cycle cost, which includes the initial cost, the costs of inspection, repair, and failure. Degradation of seismic performance is modeled by using a damage function. And failure probability is computed according to the degree of damage detection by random vibration theory and the event tree analysis. As an example to illustrate the proposed approach, a 10-span continuous bridge structure is used. The numerical results show that the optimum number of the inspection and the repair are increased, as the seismic intensity is increased and the soil condition of a site becomes more flexible.

• Transactions on Electrical and Electronic Materials
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• v.18 no.6
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• pp.350-358
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• 2017

Recently, owing to increased interest in low-carbon energy supplies, renewable energy sources such as photovoltaics and wind turbines in distribution networks have received considerable attention for generating clean and unlimited energy. The presence of energy storage systems (ESSs) in the promising field of active distribution networks (ADNs) would have direct impact on power system problems such as encountered in probabilistic distributed generation (DG) model studies. Hence, the optimal procedure is offered herein, in which the simultaneous placement of an ESS, photovoltaic-based DG, and wind turbine-based DG in an ADN is taken into account. The main goal of this paper is to maximize the net present value of the loss reduction benefit by considering the price of electricity for each load state. The proposed framework consists of a scenario tree method for covering the existing uncertainties in the distribution network's load demand as well as DG. The collected results verify the considerable effect of concurrent installation of probabilistic DG models and an ESS in defining the optimum site of DG and the ESS and they demonstrate that the optimum operation of an ESS in the ADN is consequently related to the highest value of the loss reduction benefit in long-term planning as well. The results obtained are encouraging.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.552-557
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• 2013

Partially purified ${\alpha}$-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtained. The optimum pH of the free and immobilized enzyme was 6.5 to 7.0 and 7.0, respectively, while there was no change in optimum temperature between the free and immobilized ${\alpha}$-galactosidase. The immobilized ${\alpha}$-galactosidase was reutilized six times without significant loss in activity. The immobilized enzyme showed good storage stability at $37^{\circ}C$, retaining about 50% of its initial activity even after 18 d at this temperature, while the free enzyme was completely inactivated. The immobilization of ${\alpha}$-galactosidase from Bacillus sp. LX-1 on Eudragit L-100 may be a promising strategy for removal of ${\alpha}$-galacto-oligosaccharides such as raffinose and stachyose from soybean meal and other legume in feed industry.

• Journal of the Korean Institute of Navigation
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• v.15 no.3
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• pp.1-11
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• 1991

As increasing needs of marine transportation , world merchant fleet and ship's size were enlarged and it caused frequent disasters in human lives and natural environment. By the reason of the above, they started to establish the Vessel Traffic System (VTS) at the European coast in 1960' and most of advanced contries established and managed it to prevent the sea traffic accidents in these days. The concept of traffic control at sea can be divided into three types. First, the initial gathering of informations about ship's identity and movement etc.. Second, monitoring of the traffic flow and amendment of instructions. Third , organization and direction of ships by allocating routes and speeds. Where the goal of traffic control is safety of traffics and developing effectiveness of navigation channel, if traffic volume is less tan channel capacity then the above first or second level of control would be sufficient but if it is bigger than that , more positive policy of control should be adopted as same as third type of the above. In this paper where the strategy of VTS is focused on the control of traffic density to be spread equality, as possible , all over the navigation channels and also improvement of effectiveness , it suggests algorithm to assign the vessels to the channels with balanced traffic density , and other algorithms using D.P. to sequence the vessels assigned to one channel in optimum order which decreases the mean waiting time in sense of channel effectiveness with numerical examples.

• Journal of Environmental Impact Assessment
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• v.20 no.3
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• pp.357-365
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• 2011

The QUAL2K has the same basic characteristics as the QUAL2E model, which has been widely used in stream water quality modeling; in QUAL2K, however, various functions are supplemented. The QUAL2Kw model uses a genetic algorithm(GA) for automatic calibration of QUAL2K, and it can search for optimum water quality parameters efficiently using the calculation results of the model. The QUAL2Kw model was applied to the Gangneung Namdaecheon River on the east side of the Korean Peninsula. Because of the effluents from the urban area, the middle and lower parts of the river are more polluted than the upper parts. Moreover, the hydraulic characteristics differ between the lower and upper parts of rivers. Thus, the river reaches were divided into seven parts, auto-calibration for the multiple reaches was performed using the function of the user-defined automatic calibration of the rates worksheets. Because GA parameters affect the optimal solution of the model, the impact of the GA parameters used in QUAL2Kw on the fitness of the model was analyzed. Sensitivity analysis of various factors, such as population size, crossover probability, crossover mode, strategy for mutation and elitism, mutation rate, and reproduction plan, were performed. Using the results of this sensitivity analysis, the optimum GA parameters were selected to achieve the best fitness value.

• International Journal of Precision Engineering and Manufacturing-Green Technology
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• v.5 no.5
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• pp.593-604
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• 2018

Laser-assisted machining (LAM) is known to be an effective and economical technique for improving the machinability of difficult-to-machine materials. In the LAM method, material is preheated using a laser heat source and then the preheated area is removed by following cutting tool. For laser-assisted turning (LAT), the configuration of the system is not complicated because laser irradiates from a fixed position. In contrast, laser-assisted milling (LAMill) system is not only complicated but also difficult to control because laser heat source must always move ahead of the cutting tool along a three dimensional (3D) tool path. LAMill is still early stage and cannot yet be used to machine finished products with 3D shapes. In this study, a laser-assisted fillet milling process was developed for machining 3D shapes. There are no prior studies combining fillet milling and LAMill. Laser-assisted fillet milling strategy was proposed, and effective depth of cut (EDOC) was obtained using thermal analysis. Experiments were designed using response surface method and cutting force prediction equations were developed using statistical analysis and regression analysis. The optimum machining conditions were also proposed, and energy efficiency of the LAMill was analyzed by comparing the specific cutting energy of conventional machining (CM) and LAMill.

• Korean Journal of Plant Resources
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• v.35 no.3
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• pp.399-404
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• 2022

Russian dandelion, Taraxacum kok-saghyz L.E.Rodin, is an important alternative source of natural rubber. Thus, it is critical to identify the best season for natural rubber production and harvesting for this species. In this study, T. kok-saghyz was grown in experimental fields in Korea to check the variation in rubber content with the change in the season. Studies using T. kok-saghyz plants indicate that spring and autumn are the best seasons for rubber accumulation. For several reasons, our study suggests that planting in autumn and harvesting in late spring is the best strategy for economic cultivation in Korea. Our data will be useful for future commercialization of rubber production from T. kok-saghyz because time will be saved if the plant is grown and harvested in the optimum season.