• Korean journal of food and cookery science
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• v.15 no.3
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• pp.238-243
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• 1999

For the development of technique for isolation of naked barley starch from Youngsan variety, optimum conditions of the isolation process were investigated. The effect of blending was examined and the results showed that 29.7% starch yield was obtained by 6 times of blending. After the blending, the barley starch contained 3.2% protein, 0.7% fat, 0.4% fiber, 0.4% ash and 2.8% ${eta}$-glucan. The opitmum conditions of ${eta}$-glucanase treatment were studied and the results showed that the amount of ${eta}$-glucanase and barley flour-water ratio were 60,000 unit and 1/2, the optimum steeping temperature, pH were $45^{\circ}C$ and 6.5, respectively. The effect of alkali treatment which would be supposed to increase the yield and purity of the barley starch was also examined. 76.7% starch content was obtained by 2 hr of alkali treatment. After all the treatment of isolation process, the barley starch finally contained 0.2% protein and 0.1% ${eta}$-glucan.

• Korean journal of food and cookery science
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• v.15 no.2
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• pp.185-190
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• 1999

In order to develop the techniques for isolation and production of barley starch from Youngsan variety, optimum conditions of isolation processes of barley starch was investigated. The effect of steeping temperature and time in wet-milling process was examined and the results showed that optimal steeping temperature and time were 30$^{\circ}C$ and 12 hr. The barley starch isolated under these conditions contained 5.7% crude protein, 69% starch content and 83% white value by 100 mesh process. The optimum condition of alkali treatment was showed that concentration of NaOH and treating time were 0.2% and 6 hr, respectively. To remove the fat content of barley starch, after alkali process obtained barley starch with the addition of 10% (v/v) EtOH was attempted. As the result, the barley starch contained 0.1% of crude fat. Under the optimized isolation conditions, the barley starch finally contained 0.1% protein and 95% starch content. The isolated barley starch were superior to commercial corn starch in purity and white value.

• Earthquakes and Structures
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• v.16 no.6
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• pp.743-755
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• 2019

In this paper, a new system of seismic isolation for buildings - called suspended columns - is introduced. In this method, the building columns are placed on the hinged cradle seats instead of direct connection to the foundation. In this system, each of the columns is put on a seat hung from its surrounding area by a number of cables, for which cavities are created inside the foundation around the columns. Inside these cavities, the tensile cables are hung. Because of the flexibility of the cables, the suspended seats vibrate during an earthquake and as a result, there is less acceleration in the structure than the foundation. A Matlab code was written to analyze and investigate the response of the system against the earthquake excitations. The findings showed that if this system is used in a building, it results in a significant reduction in the acceleration applied to the structure. A shear key system was used to control the structure for service and lateral weak loads. Moreover, the effect of vertical acceleration on the seismic behavior of the system was also investigated. Effect of the earthquake characteristic period on the system performance was studied and the optimum length of the suspension cables for a variety of the period ranges was suggested. In addition, measures have been taken for long-term functioning of the system and some practical feasibility features were also discussed. Finally, the advantages and limitations of the system were discussed and compared with the other common methods of seismic isolation.

• Geomechanics and Engineering
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• v.31 no.1
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• pp.1-14
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• 2022

Urban deep excavation will affect greatly on the deformation of adjacent existing buildings, especially those with shallow foundations. Isolation piles has been widely used in engineering to control the deformation of buildings adjacent to the excavation, but its applicability is still controversial. Based on a typical engineering, numerical calculation models were established and verified through monitoring data to study the influence characteristics of isolation piles on the deformation of existing shallow foundation buildings. Results reveal that adjacent buildings will increase building settlement δ_v and the deformation of diaphragm walls δ_h, while the isolation piles can effectively decrease these. The surface settlement curve is changed from "groove" type to "double groove" type. Sufficiently long isolation pile can effectively decrease δ_v, while short isolation piles will lead to a negative effect. When the building is within the range of the maximum settlement location P, maximum building rotation θ_m will increase with the pile length L and the relative position between isolation pile and building d/D increase (d is the distance between piles and diaphragm walls, D is the distance between buildings and diaphragm walls), instead, θ_m will decrease for buildings outside the location P, and the optimum was obtained when d/D=0.7.

• Food Science of Animal Resources
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• v.35 no.4
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• pp.431-440
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• 2015

Chicken bone is not adequately utilized despite its high nutritional value and protein content. Although not a common raw material, chicken bone can be used in many different ways besides manufacturing of collagen products. In this study, a multi-step procedure was optimized to isolate chicken bone collagen for higher yield and quality for manufacture of collagen products. The chemical composition of chicken bone was 2.9% nitrogen corresponding to about 15.6% protein, 9.5% fat, 14.7% mineral and 57.5% moisture. The lowest amount of protein loss was aimed along with the separation of the highest amount of visible impurities, non-collagen proteins, minerals and fats. Treatments under optimum conditions removed 57.1% of fats and 87.5% of minerals with respect to their initial concentrations. Meanwhile, 18.6% of protein and 14.9% of hydroxyproline were lost, suggesting that a selective separation of non-collagen components and isolation of collagen were achieved. A significant part of impurities were selectively removed and over 80% of the original collagen was preserved during the treatments.

• The Korean Journal of Food And Nutrition
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• v.13 no.4
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• pp.379-382
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• 2000

Conditions of isolation for the red color pigments from the Korean Lithospermum erythrorhizon were investigated and identification of the red pigment was analysed. Non-polar solvents were more effective than water. Especially, 95% ethanol was observed as optimum solvent for the pigments extraction. About 20 minutes at 40$^{\circ}C$ with 95% ethanol was enough for the extraction of the red pigments. The major pigment was analysed as acetylshikonin by TLC, IR, NMR and GC/MS.

• Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.259-263
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• 2012

To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoalteromonas sp., based on morphological, biochemical, 16S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalteromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginate-degrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at $30^{\circ}C$. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.

• ETRI Journal
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• v.35 no.4
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• pp.718-721
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• 2013

This letter proposes a novel calibration method for a multiport amplifier (MPA) to achieve optimum port-to-port isolation by correcting both the amplitude and phase of the calibration signals. The proposed architecture allows for the detection of the phase error and amplitude error in each RF signal path simultaneously and can enhance the calibrated resolution by controlling the analog phase shifters and attenuators. The designed $2{\times}2$ and $4{\times}4$ MPAs show isolation characteristics of 30 dB and 27 dB over a frequency range of 19.5 GHz to 22.5 GHz, respectively.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.11b
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• pp.755-760
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• 2001

Major equipment of combat vessels normally require the shock and vibration isolation to maintain its performance against environments of severe vibration and shock. In this paper, an optimal layout design method of resilient mounts for shock and vibration isolation is presented using simulated annealing optimization method. The reference levels of vibration and shock to isolate shipboard equipment are determined from the MIL-STD-167-1 and MIL-S-901 specifications. Through the numerical application, the validity of the presented method is investigated.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1171-1177
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• 2015

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.