• Journal of Veterinary Science
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• v.23 no.3
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• pp.48.1-48.12
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• 2022

Background: The poor intracellular concentration of enrofloxacin might lead to treatment failure of cow mastitis caused by Staphylococcus aureus small colony variants (SASCVs). Objectives: In this study, enrofloxacin composite nanogels were developed to increase the intracellular therapeutic drug concentrations and enhance the efficacy of enrofloxacin against cow mastitis caused by intracellular SASCVs. Methods: Enrofloxacin composite nanogels were formulated by an electrostatic interaction between gelatin (positive charge) and sodium alginate (SA; negative charge) with the help of CaCl₂ (ionic crosslinkers) and optimized by a single factor test using the particle diameter, zeta potential (ZP), polydispersity index (PDI), loading capacity (LC), and encapsulation efficiency (EE) as indexes. The formation mechanism, structural characteristics, bioadhesion ability, cellular uptake, and the antibacterial activity of the enrofloxacin composite nanogels against intracellular SASCVs strain were studied systematically. Results: The optimized formulation was comprised of 10 mg/mL (gelatin), 5 mg/mL (SA), and 0.25 mg/mL (CaCl₂). The size, LC, EE, PDI, and ZP of the optimized enrofloxacin composite nanogels were 323.2 ± 4.3 nm, 15.4% ± 0.2%, 69.6% ± 1.3%, 0.11 ± 0.02, and -34.4 ± 0.8 mV, respectively. Transmission electron microscopy showed that the enrofloxacin composite nanogels were spherical with a smooth surface and good particle size distributions. In addition, the enrofloxacin composite nanogels could enhance the bioadhesion capacity of enrofloxacin for the SASCVs strain by adhesive studies. The minimum inhibitory concentration, minimum bactericidal concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were 2, 4, 4, and 8 ㎍/mL, respectively. The killing rate curve had a concentration-dependent bactericidal effect as increasing drug concentrations induced swifter and more radical killing effects. Conclusions: This study provides a good tendency for developing enrofloxacin composite nanogels for treating cow mastitis caused by intracellular SASCVs and other intracellular bacterial infections.

• Composites Research
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• v.33 no.6
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• pp.371-376
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• 2020

The adhesive strength can be improved through surface treatment. The most common method is to improve physical bonding by varying the surface conditions. This study presents the effect of laser surface treatment on the adhesive strength of CFRP. The surface roughness was patterned using a 1064 nm laser. The effects of the number of laser shots and the direction and length of the pattern on the adhesion of the CFRP/CFRP single joint were investigated through tensile tests. Tests according to ASTM D5868 were performed, and the bonding mechanism was determined by analyzing the damaged surface after a fracture. The optimized number of the laser shots and the optimized depth of the roughness should be required to increase the bonding strength on the CFRP surface. When considering the shear stress in the tensile direction, the roughness pattern in the direction of 45° that increases the length of the fracture path in the adhesive layer resulted in an increase of the adhesive strength. The surface treatment of the bonding surface using a laser is a suitable method to acquire a mechanical bonding mechanism and improve the bonding strength of the CFRP bonding joint. The study on the optimized laser process parameters is required for utilizing the benefits of laser surface processing.

• Steel and Composite Structures
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• v.29 no.3
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• pp.301-308
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• 2018

Properties of AFS vary with the changes in the face-sheet materials. Hence, the performance of AFS can be optimized by selecting face-sheet materials. In this work, three types of face-sheet materials representing elastic-perfectly plastic, elastic-plastic strain hardening and purely elastic materials were employed to study their effects on the flexural behavior and failure mechanism of AFS systematically. Result showed face-sheet materials affected the failure mechanism and energy absorption ability of AFS significantly. When the foam cores were sandwiched by aluminum alloy 6061, the AFS failed by face-sheet yielding and crack without collapse of the foam core, there was no clear plastic platform in the Load-Displacement curve. When the foam cores were sandwiched by stainless steel 304 and carbon fiber fabric, there were no face-sheet crack and the sandwich structure failed by core shear and collapse, plastic platform appeared. Energy absorption abilities of steel and carbon fiber reinforced AFS were much higher than aluminum alloy reinforced one. Carbon fiber was suggested as the best choice for AFS for its light weight and high performance. The versus strength ratio of face sheet to core was suggested to be a significant value for AFS structure design which may determine the failure mechanism of a certain AFS structure.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.806-811
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• 2005

The robot to search and rescue is used in narrow space where human cannot approach. In case of this robot, it can overcome obstacles such as wrecks or stairs etc. Also, this robot can do various locomotion for each object. In this reason, an articulated robot has advantages comparing with one module robot. However, the existing articulated robot has limits to overcome vertical passages. For expanding contacted territory of robot, a novel mechanism is demanded. In this paper, the novel mechanism of articulated mobile robot is designed for moving level ground and vertical passages. This paper proposes to change wheel alignment. The robot needs two important motions for passing vertical passages like pipe. One is a motion to press wheels at wall for not falling into gravity direction. The other is a motion that wheels contact a vertical direction of wall's tangential direction for reducing loss of force. The mechanism of the robot focused that two motions can be acted to use just one motor. Length of each link of robot is optimized that wheels contact a vertical direction of wall's tangential direction through kinematic modeling of each link. The force of pressing wall of robot is calculated through dynamic modeling. This robot composes four modules. This mechanism is confirmed by dynamic simulation using ADAMS program. The articulated mobile robot is elaborated based on the results of kinematic modeling and dynamic simulation.

• Industrial Engineering and Management Systems
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• v.15 no.2
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• pp.156-172
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• 2016

Complex and uncertain issues in supply chain result in integrated decision making processes in supply chains. So decentralized (distributed) decision making (DDM) approach is considered as a crucial stage in supply chain planning. In this paper, an uncertain DDM through coordination mechanism is addressed for a multi-product supply chain planning problem. The main concern of this study is comparison of DDM approach with centralized decision making (CDM) approach while some parameters of decision making are assumed to be uncertain. The uncertain DDM problem is modeled through fuzzy mathematical programming in which products' demands are assumed to be uncertain and modeled using fuzzy sets. Moreover, a CDM approach is customized and developed in presence of fuzzy parameters. Both approaches are solved using three fuzzy mathematical optimization methods. Hence, the contribution of this paper can be summarized as follows: 1) proposing a DDM approach for a multi-product supply chain planning problem; 2) Introducing a coordination mechanism in the proposed DDM approach in order to utilize the benefits of a CDM approach while using DDM approach; 3) Modeling the aforementioned problem through fuzzy mathematical programming; 4) Comparing the performance of proposed DDM and a customized uncertain CDM approach on multi-product supply chain planning; 5) Applying three fuzzy mathematical optimization methods in order to address and compare the performance of both DDM and CDM approaches. The results of these fuzzy optimization methods are compared. Computational results illustrate that the proposed DDM approach closely approximates the optimal solutions generated by the CDM approach while the manufacturer's and retailers' decisions are optimized through a coordination mechanism making lasting relationship.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.1
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• pp.71-78
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• 2014

Door hinges of large refrigerators are required to ensure that the doors open and close smoothly in addition to supporting door weights and enduring the impact loads due to door opening and closing. However, door hinge design is difficult because of complex hinge mechanisms and sensitive structural safety. In this study, the mechanism satisfying the required spring response, space constraints, and structural strength is optimized, and the volume of the outer frame covering the hinge mechanism is minimized for reducing production costs. The entire design process is automated using the PIDO(Progress Integration and Design Optimization) technique, which achieves an efficient design process. Therefore, the frame mass is reduced to 24%, and the mechanism performance and structural stability are improved.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1428-1438
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• 2016

In the present work, Arthrobacter sp. 25, a lead-tolerant bacterium, was assayed to remove lead(II) from aqueous solution. The biosorption process was optimized by response surface methodology (RSM) based on the Box-Behnken design. The relationships between dependent and independent variables were quantitatively determined by second-order polynomial equation and 3D response surface plots. The biosorption mechanism was explored by characterization of the biosorbent before and after biosorption using atomic force microscopy (AFM), scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The results showed that the maximum adsorption capacity of 9.6 mg/g was obtained at the initial lead ion concentration of 108.79 mg/l, pH value of 5.75, and biosorbent dosage of 9.9 g/l (fresh weight), which was close to the theoretically expected value of 9.88 mg/g. Arthrobacter sp. 25 is an ellipsoidal-shaped bacterium covered with extracellular polymeric substances. The biosorption mechanism involved physical adsorption and microprecipitation as well as ion exchange, and functional groups such as phosphoryl, hydroxyl, amino, amide, carbonyl, and phosphate groups played vital roles in adsorption. The results indicate that Arthrobacter sp. 25 may be potentially used as a biosorbent for low-concentration lead(II) removal from wastewater.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2648-2653
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• 2003

This paper describes the development of a 3-DOF laparoscopic assistant robot system with motor-controlled bending and zooming mechanisms using the voice command motion control and auto-tracking control. The system is designed with two major criteria: safety and adaptability. To satisfy the safety criteria we designed the robot with optimized range of motion. For adaptability, the robot is designed with compact size to minimize interference with the staffs in the operating room. The required external motions were replaced by the bending mechanism within the abdomen using flexible laparoscope. The zooming of the robot is achieved through in and out motion at the port where the laparoscope is inserted. The robot is attachable to the bedside using a conventional laparoscope holder with multiple DOF joints and is compact enough for hand-carry. The voice-controlled command input and auto-tracking control is expected to enhance the overall performance of the system while reducing the control load imposed on the surgeon during a laparoscopic surgery. The proposed system is expected to have sufficient safety features and an easy-to-use interface to enhance the overall performance of current laparoscopy.

• The Transactions of the Korea Information Processing Society
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• v.7 no.9
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• pp.2980-2993
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• 2000

In order to achieve the rapid deployment of services. B-ISON network is being combined with terrestrial ATM and satellite network. Cell delay variation (CDV) generated by the difference of transfer mode between TOMA and ATM deteriorates transmission quality of the network system. We proposed the Partial Timestamps algorithm to supplement the problems of existing COV compensation methods. To minimize CDV and to utilize the satellite channels efficiently. only the optimized timestamps of a few cells within a control unit time of TDMA are selected and transmitted to the receiving earth station. The COV compensating efficiency of Partial Timestamps is evaluated by simulation. It is confirmed that CDV compensation capability of the proposed mechanism is superior to the other methods.

• The Journal of the Acoustical Society of Korea
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• v.14 no.2
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• pp.73-79
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• 1995

Most of conventional ultrasonic transducers are constructed to generate either longitudinal or shear waves, but not both of them. We investigated the mechanism of dual mode transducers that generates both of the longitudinal and shear waves simultaneously with a single PZT element. A piezoelectric ceramic PZT has the hexagonal 6mm crystal symmetry, after poling. We studied the performance of a PZT element as a function of its rotation angle so that its efficiency is optimized to excite the two waves equally strongly. The results are verified by checking the impedance variation of the element with Finite Element Methods, and checking the wave form by pulse-echo test simulation. Validity of the theoretical calculation is verified through experiments.