• Mycobiology
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• 제45권3호
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• pp.204-208
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• 2017

Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2.95 times compared with the use of common 3 mm beads alone. The optimal time for the enzyme treatment was determined to be 1 hr as measured by the yield and viability of the spores. A one-step filtration using a filter paper with an $8-11{\mu}m$ pore size was sufficient for removing cell debris. This method may be useful to purify not only N. ceranae spores but also other Nosema spp. spores.

• International Journal of Air-Conditioning and Refrigeration
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• 제6권
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• pp.124-135
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• 1998

A thermodynamic cycle analysis is performed for refrigerator-precooled Linde-Hampson hydrogen liquefiers, including catalysts for the ortho-to-para conversion. Three different configurations of the liquefying system, depending upon the method of the o-p conversion, are selected for the analysis. After some simplifying and justifiable assumptions are made, a general analysis program to predict the liquid yield and the figure of merit (FOM) is developed with incorporating the commercial computer code for the thermodynamic properties of hydrogen. The discussion is focused on the effect of the two primary design parameters - the precooling temperature and the high pressure of the cycle. When the precooling temperature is in a range between 45 and 60 K, the optimal high pressure for the maximal liquid yield is found to be about 100 to 140 bar, regardless of the ortho-to-para conversion. However, the FOM can be maximized at slightly lower high pressures, 75 to 130 bar. It is concluded that the good performance of the precooling refrigerator is significant in the liquefiers, because at low precooling temperatures high values of the liquid yield and the FOM can be achieved without compression of gas to a very high pressure.

• Bulletin of the Korean Chemical Society
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• 제34권10호
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• pp.3059-3064
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• 2013

In this study transesterification of Triglycerides (TG) from Jatropha curcas oil (JCO) with methanol for production of biodiesel was investigated over cerium impregnated ZSM-5 catalysts. NaZSM-5 was synthesized in an alkaline medium and impregnated with cerium oxide by wet method using cerium nitrate as a source for cerium. They were characterized by X-ray diffraction (XRD), Thermogravimeteric analysis (TGA), $CO_2$-temperature programmed desorption, and $N_2$ adsorption/desorption analysis. XRD analysis showed decrease in intensity of the patterns with the increase in the ceria loading but crystallization of ceria to larger size is an evident for 10 and 15% loading. The optimal yield of transesterification process was found to be 90% under the following conditions: oil to methanol molar ratio: 1:12; temperature: $60^{\circ}C$; time: 1 h; catalyst: 5 wt %. Here the yield of fatty acid methyl ester (FAME) was calculated through $^1H$ NMR analysis. The investigation on catalyst loading, temperature, time and reusability illustrated that these ceria impregnated NaZSM-5's were found to be selective, recyclable and could yield biodiesel at low temperature with low methanol to oil ratio due to the presence of both Lewis and Bronsted basicity. Hence, from the above study it is concluded that ceria impregnated ZSM-5 could be recognized as a potential catalysts for biodiesel production in industrial processes.

• Plant Resources
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• 제8권3호
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• pp.258-262
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• 2005

In autumn, to determine the optimal planting date and plant density of rapeseed in southern areas of Korea, Yudal variety for autumn sowing, the highest yielding variety was grown under three different planting dates and five different plant densities. Yield components such as plant height, ear length, number of seedling stand per $m^2$, number of branches and pod length were highest at the plots with Sep. 30 of planting date and 30/20 cm drilling of plant density. Yield of seed, oil and 1,000 grains weight were highest at the Sep.30 of planting date and 30/20 cm drilling of plant density. Judging from the results reported above, at optimum planting date and plant density of rapeseed seemed to be Sep.30 of planting date and 30/20cm plant density in autumn sowing.

• 수산해양교육연구
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• 제17권3호
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• pp.340-360
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• 2005

Fishery resource rents(i.e. windfall gains or excess profit), which tend to lead a variety of important economic, social, political problems, have been a fundamental cause of unbalance between fishery resource use and management. Thus, there may exist several sorts of optimal level of resource utilization such as economic maximum sustainable yield, biological maximum sustainable yield, social optimum production, socio-political optimum yield, etc. The fishery resource use level seems to a large extent to be determined by the characteristics of fishermen's rent seeking structure. As well known, fishery resources as common properties have a characteristic of being difficult to establish private property rights. Therefore, their use rights are controlled by the permit and/or the license system. As a result, absolute or differential rents are formed by the changes in institutional arrangements. Rent problems are often transformed into serious socio-political issues when the rent in a given industry is much higher to a socially unacceptable extent than the average of other industries. However, individual fishermen or fishermen's groups tend to behave aggressively to change the existing fishery institutions towards maximizing fishery rents. These rent-seeking behaviors often tend to nullify fishery management schemes. The larger is the relative rent difference between fisheries and other industries, the more aggressive tend to be the rent-seeking behaviors in fisheries.

• Plant Resources
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• 제3권3호
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• pp.194-199
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• 2000

The result of this experiment which are conducted, to improve the cultivation technology of Alisma plantago, to increase its quantity and to contribute for stable production with Yongiun local group by examining the optimal planting density and transplanting period of double cropping of Alisma plantago in the southern region. The characters of plant height, leaf width and length tend to be reduced as the seeding period is later by the order of the 10th, 20th and 30th of July. The period required for flowering is reduced as the transplanting period is later and dense planting is applied. Plant height, the number of leaves and yield of dry root have much quantity at the dense planting density of 20$\times$ 15cm as they are transplanted later in the 30th of August or the 10th of September, but they are rather less in sparse planting density of 20$\times$25cm or 20$\times$35cm.

• 한국초지조사료학회지
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• 제12권2호
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• pp.123-126
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• 1992

남부지방에 적합한 청예사초용 율무의 파종적기를 구명코자 다수성으로 인정된 승주 재래종을 공시하여 시험했던 바 그 결과를 요약하면 다음과 같다. 1. 초장, 경태, 엽수 등의 수량 구성형질은 4월 15일에 파종한 구가 가장 우수하였다. 2. 생초수량과 건물수량은 역시 4월 15일에 파종한 구가 증수되었다. 3. 조단백질 함량은 파종기가 늦어질수록 높았고 NDF, ADF 등의 조섬유 함량은 파종기가 늦어질수록 저하되었다.

• Membrane and Water Treatment
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• 제6권4호
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• pp.277-292
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• 2015

The extraction of lactic acid by an emulsion liquid membrane (ELM), in batch and continuous mode, has been reported. On the basis of preliminary experiments, the optimum composition of the organic phase (membrane) is determined. When the SPan 80 is used as surfactant, the emulsion breakage exceeds 50%, but only 10% is obtained when the ECA4360 is used. The effects of surfactant, carrier and solute concentrations, phase volume ratio, and stirring speed on the extraction yield were examined and optimized. Surfactant, carrier and diluent used were ECA4360, trilaurylamine (TLA) and dodecane, respectively; 2-ethylhexane-1,3-diol (EHD) is used as a co-surfactant. Under optimal conditions, emulsion breaking is very low and the swelling is kept at its lowest level. Under the pH conditions of fermentation medium, the extraction yield is lower. A mixer-settler continuous system was used for testing these conditions. The residence time, the number of extraction stages and the stability of the emulsion were studied and optimized. The extraction yield obtained exceeds 90%.

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.1054-1063
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• 2022

Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell Pseudomonas monteilii TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Triton X-100 permeabilized cells. According to our central composite design (CCD) experiment, the optimal process was achieved at 35℃ and pH 8.0 for 24 h, resulting in the maximum trehalose yield of 51.60 g/g after 12 h using an initial cell loading of 94 g/l. Scale-up production in a lab-scale bioreactor led to the final trehalose concentration of 51.91 g/l with a yield of 51.60 g/g and productivity of 4.37 g/l/h together with 8.24 g/l glucose as a byproduct. A one-pot process integrating trehalose production and byproduct bioremoval showed 53.35% trehalose yield from 107.4 g/l after 15 h by permeabilized P. moteilii cells. The residual maltose and glucose were subsequently removed by Saccharomyces cerevisiae TBRC 12153, resulting in trehalose recovery of 99.23% with 24.85 g/l ethanol obtained as a co-product. The present work provides an integrated alternative process for trehalose production from maltose syrup in bio-industry.

• Food Science and Biotechnology
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• 제15권5호
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• pp.814-816
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• 2006

Monacolin K (MK) was produced on soybean using Monascus ruber GM011 by a two-stage-fermentation process. The optimal temperature was identified as $28^{\circ}C$. Higher yield was obtained by multiple-level-temperature cultivation than by single-level-temperature cultivation. The highest yield of total MK, 4.810 mg/g dry soybean product, was attained after 30 days of solid-state fermentation. No citrinin could be detected in the fermented soybean.