• Natural Product Sciences
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• v.21 no.2
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• pp.141-145
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• 2015

Hesperidin, which is the most abundant flavonoid of Citrus unshiu (Rutaceae), has been reported to possess diverse activities and widely used as functional foods and cosmetics. For the development of functional products, extraction procedure is indispensable. Extraction conditions affect the composition of extract as well as its biological activity. Therefore, we tried to optimize extraction conditions such as extraction solvent, extraction time and extraction temperature for maximum yield of hesperidin using response surface methodology with threelevel-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 59.0%; temperature $71.5^{\circ}C$ and extraction time, 12.4 h. The hesperidin yield under the optimal condition was found to be $287.8{\mu}g$ per 5 mg extract, which was well matched with the predicted value of 290.5 μg. These results provides optimized extraction condition for hesperidin and might be useful for the development of hesperidin as functional products like health supplements, cosmetics and medicinal products.

• Microbiology and Biotechnology Letters
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• v.15 no.5
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• pp.340-345
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• 1987

Microorganisms capable of utilizing D-xylose as a sole carbon and energy source were isolated to ferment D-xylose directly to ethanol. Among them, the strain, which showed the best ability to pro-duce ethanol, was selected and was identified as Fusarium sp. The optimal conditions for the pro-duction of ethanol were 8.0 of initial pH, 33$^{\circ}C$ of temperature, and 2% of substrate concentration. Under this optimal condition, the following results were obtained : maximum ethanol concentration, 7.0g/$\ell$; ethanol yield, 0.35g of ethanol per g of D-xylose (68.6% of theoretical); biomass yield, 0.27g of dry biomass per g of D-xylose.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.272-279
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• 2004

The process for the preparation of fungicide propineb starting with 1,2-diaminopropane was studied on the optimal condition base. Side reaction producing toxic material 1,2-propylenethiourea could be ieduced effectively by using phase transfer catalyst and the product was noticed to show a great improvement in yield and purity. Especially when the phase transfer catalyst tetraphenylphosphonium bromide is used, the yield and the purity of the product were found to be best with up to 95 and 96% respectively and the byproduct content was shown within 1.7%. Also, the contents of wetting agent AES and dispersing agent APS were optimally chosen $3.1\sim4.0%$ and $4.0%\sim5.0%$ respectively for the improvement of suspensibility and wettability of Propineb WP.

• Natural Product Sciences
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• v.24 no.1
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• pp.71-78
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• 2018

The present study deals with the determination of optimal values of operating parameters such as the temperature of heating, the mass of the plant material and the volume of water leading to the best yield of electromagnetic induction (EMI) heating extraction of Algerian Thymus fontanesii essential oil. After an appropriate choice of the three critical variables, eight experiments leaded to a mathematical model as a first-degree polynomial presenting the response function (yield) in the relation to the operating parameters. From the retained model, we were able to calculate the average response, the different effects and their interactions. The maximum of essential oil recovery percentage relative to the initial mass of plant material was 1.69%, and was obtained at ($140^{\circ}C$, 250 g and 4.5 L). The chemical composition of the Algerian T. fontanesii essential oil under the obtained optimal conditions ($140^{\circ}C$, 250 g and 4.5 L), determined by GC/MS and GC/FID, reveled of the presence of major components such as: carvacrol ($70.6{\pm}0.1%$), followed by p-cymene ($8.2{\pm}0.2%$).

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.4
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• pp.18-27
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• 1997

This study was performed to obtained the optimal delignified condition of exploded wood on the acid hydrolysis with sulfuric acid. Wood chips of pine wood(Pinus desiflora), oak wood(Quercus serrata) and birch wood (Betula platyphylla var. japonica) were treated with a high pressure steam (20-30kgf/$\textrm{cm}^2$, 2-6 minutes). The exploded wood was delignified with sodium hydroxide and sodium chlorite, and then hydrolyzed with sulfuric acid. The result can be summerized as follows ; In the exploded wood treated with sodium hydroxide, the optimal concentration of sodium hydroxide was 1% as content of lignin in the exploded wood. Lignin content of exploded wood treated with sodium chlorite was lower then that sodium hydroxide. The maximum reducing sugar yield of exploded wood treated with 1% sodium hydroxide was lower than non-treated exploded wood. In the case of sodium chlorite treated, the maximum reducing sugar yield was hgher than non-treated exploded wood. Sugar composition of acid hydrolysis solution was composed of xylose and glucose residue, and the rate of glucose residue was increased in high pressure condition.

• Korean Journal of Microbiology
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• v.28 no.4
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• pp.345-350
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• 1990

In order to ferment D-xylose directly to ethanol, Yeasts capable of utilizing D-xylose as a sole carbon source and energy source were isolated from soil, sawdust and rotten woods. Among them, the yeast strain, which showed the best ability to produce ethanol, was identified as Candida sp. L-16 isolated from rotten woods. The optimal conditions for production of ethanol were 60rpm of agitation speed, 28j.deg.C of temperature, 4.5 of initial pH and 5% of D-xylose concentration. Ethanol production was reached to maximum state for 4 days culture. Under these optimal conditions, the maximum ethanol concentration and theoretical ethanol yield were 2.4%(v/v) and 74.4% of theoretical value, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.5
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• pp.286-290
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• 2003

The effect of thinned fruits, apple, pear and peach, on the mycelial growth of mushrooms was investigated. The growth of mycelia with the addition of thinned fruit was clearly better than that in the control for all the tested mushrooms. The growth rate of Pleurotus ostreatus was faster than any other mushroom. The optimal concentrations of thinned apple, pear, and peach in a solid culture were 1.0%, 1.0%, and 3%, respectively, while in a liquid culture the optimal concentrations were 5,0%, 3.0%, and 5.0%, respectively. When Pleurotus ostreatus was incubated in a 20-L pilot scale fermenter with 10 L of a liquid medium containing 3% thinned fruit at 25$^{\circ}C$ and 6 vvm for 10 days, the mass-production of mycelia was 74.2 g/10 L (apple), 96.2 g/10 L (pear), and 86.3 g/10 L (peach). The mycelial yield of Pleurotus ostreatus in a medium containing thinned fruit was 2 ∼ 3 times higher than that in the control.

• Microbiology and Biotechnology Letters
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• v.24 no.4
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• pp.507-511
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• 1996

Medium optimization for xylitol production from xylose by Candida parapsilosis ATCC 22019 mutant was performed. Effect of various nitrogen sources on xylitol production was investigatied. Of inorganic nitrogenous compounds, ammonium sulfate was effective for xylitol production and yeast extract was the most suitable orangic nitrogen nutrient for enhancement of xylitol production. Effect of inorganic salts such as KH$_{2}$PO$_{4}$ and MgSO$_{4}$-7H$_{2}$0 on xylitol production was also studied. Optimal medium was selected as xylose of 50 g/l, yeast extract of 5 g/l, (NH4$_{4}$)$_{2}$SO$_{4}$ of 5 g/l, KH$_{2}$PO$_{4}$ of 5 g/l, MgSO$_{4}$-7H$_{2}$O of 0.2 g/l. In a fermentor by using the optimal medium, a final xylitol concentration of 37 g/l could be obtained from 50 g/l of xylose with a xylitol yield of 74% and a xylitol productivity of 0.58 g/1-hr. At 300 g/l xylose, fermentation was also carried out and then a final xylitol concentration of 242 g/l was obtained at 272 hours. It was corresponding to xylitol yield of 80.7% and xylitol productivity of 0.58 g/1-hr.

• YAKHAK HOEJI
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• v.33 no.6
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• pp.365-371
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• 1989

Microbiological conversion of sterols to 17-ketosteroids has been recognized as a source for commercial preparation of steroidal drugs. In order to develop bacterial strains and process with Brevibacterium lipolyticum IAM 1398 capable of converting cholesterol to 1,4-Androstadiene-3,17-dione (ADD) at about 27% yield, we studied on strain improvement, fermentation condition and whole cell immobilization. By using UV and/or NTG as mutagens, a mutant to convert cholesterol to ADD with higher yield than 60% was selected. Better production of ADD was manifested in the case of maltose used as a supplemental carbon source, and yeast extract or soytone as a nitrogen source. Addition of tween 80 (0.05%) as a surfactant beneficial for increasing the productivity. The optimal initial pH of the medium was 6.5 and optimal culture temperature was $30^{\circ}C$. Whole cell immobilization by using carrageenan, agar, alginate and acrylamide was carried out and the activity of conversion was tested. In the case of carrageenan and agar, immobilized cells were active for at least two cycles of fermentation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.98-98
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• 2019

This study was expand the use of Citrus madurensis (Citrofortunella microcarpa), which is a good source of skin whitening and antioxidants activation cosmetics. The goal was to provide cosmeceutical activity data about the extraction yield and total phenol of this calamondin orange by optimizing the focused high ultrasound(INEFU) and ultrasound extraction(UE) conditions. Under optimal extraction conditions, which consisted of 1800W for 45min and 500W for 45 min, 21.55 and 13.27 % (w/w) of the highest extractions yield and polyphenol contents were obtained. For the skin whitening activity, tyrosinase inhibitory activity was observed at 69.24 % in the INEFU extracts, which was ca. 18 % higher than that of the UE extracts (58.82 %). To reduce melanin production in Clone M-3 cells, 86.9 % melanin production was observed following the addition of control, WE(water extraction) relative to the control without a sample, and 81.2 and 78.9 % were found in the UE and INEFU conditions. The highest total phenol secretion was conclusively obtained under the optimal conditions and resulted in a significant improvement of the cosmetic activities of Citrus madurensis(Citrofortunella microcarpa) extracts.