• Title/Summary/Keyword: Optical magnification

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Multi-focal Microscopic System Using a Fiber Bundle (광섬유 다발을 이용한 다초점 현미경)

  • Gu, Young-Mo;Ham, Hyo-Shick;Choi, Sung-Eul
    • Korean Journal of Optics and Photonics
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    • v.20 no.6
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    • pp.354-360
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    • 2009
  • We have constructed and analyzed the performance of a simple fiber bundle multi-focal microscope. The microscope had a fiber bundle substituted for micro-lens array that is the core part of MMM(multi-focal multi-photon microscope). The MMM is a type of confocal microscope. To analyze the performance and characteristics of the fiber bundle multi-focal microscope, three types of samples were used: a standard grating, USAF 1951(7, 3), and 1951(7, 6). Using two polarizers and a polarizing beam splitter, we eliminated noise and got clear images. We obtained the FWHM of fiber spot images with the standard grating using two different magnifier lenses which were 63X and 20X, and found an image of the sample as a distribution of fiber spot images. For this case we used the low magnification lens, which gives denser distribution, so that we could get clearer images. In order to test the resolution of the fiber bundle multi-focal microscopic system, we used the USAF 1951 sample which has a smaller line interval than that of the standard grating. The FWHM of the line width of the image coincides well with the real line width of the USAF 1951 sample. We confirmed the performance of a fiber bundle multi-focal microscopic system which is relatively simple but has submicron resolution and is able to get 1600 images at the same time.

Advanced LWIR Thermal Imaging System with a Large Zoom Optics (줌 광학계를 이용한 원적외선 열상장비의 설계 및 제작)

  • Hong, Seok-Min;Kim, Hyun-Sook
    • Korean Journal of Optics and Photonics
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    • v.16 no.4
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    • pp.354-360
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    • 2005
  • A high performance LWIR(long wavelength infra red) zoom thermal imaging sensor using $480{\times}6$ HgCdTe(MCT) linear detector has been developed by ADD Korea. The optical system consists of zoom telescope having large objective about 190 mm diameter and optically well corrected scanning system. The zoom ratio of the telescope is 3: 1 and its magnification change is performed by moving two lens groups. And also these moving groups are used for athermalization of the system. It is certain that the zoom sensor can be used in wide operating temperature range without any degradation of the system performance. Especially, the sensor image can be displayed with the HDTV(high definition television) format of which aspect ratio is 16:9. In case of HDTV format, the scanning system is able to display 620,000 pixels. This function can make wider horizontal field of view without any loss of performance than the normal TV format image. The MRTD(minimum resolvable temperature difference) of the LWIR thermal imaging sensor shows good results below 0.04 K at spatial frequency 2 cycles/mrad and 0.23 K at spatial frequency 8 cycles/mrad at the narrow field of view.

CHANGE OF VASOACTIVE INTESTINAL POLYPEPTIDE(VIP) IMMUNOREACTIVE CELLS FOLLOWING PULP EXTIRPATION IN RAT TRIGEMINAL GANGLION: A CONFOCAL LASER SCANNING MICROSCOPIC STUDY (치수제거 후 흰쥐 삼차신경절에서 VIP 면역반응세포의 변화: 공초점레이저주사현미경적 연구)

  • Kim, Heung-Joong;Kim, Seung-Jae;Park, Joo-Cheol;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.1
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    • pp.25-31
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    • 2001
  • The purpose of this study was to investigate the distribution and fluorescence intensity of vasoactive intestinal polypeptide immunoreactive (VIP-IR) cells in rat trigeminal ganglion following pulp extirpation of rat mandibular molar. The animals were divided into control group(n=6) and experimental group(n=6). The experimental animals were sacrificed at 14 days after pulp extirpation. The trigeminal ganglion was removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer. Serial frozen sections about $20{\mu}m$ in thickness were cut with a cryostat. The immunofluorescence staining was performed. The rabbit anti-VIP(1 : 8,000) was used as primary antibody and fluorescene isothiocynate(FITC) conjugated anti-rabbit IgG(1 : 80) as secondary antibody. The slides were observed under confocal laser scanning microscope (CLSM). Unprocessed optical sections were obtained and stored on a optical disk. Color pictures were printed by a video copy processor. The results were as follows; 1. The positive ratio of VIP-IR cells in mandibular part of trigeminal ganglion were 7.40% in control group and 28.42% in experimental group(14 days after pulp extirpation). 2. The relative fluorescence intensity of VIP-IR cells in mandibular part of trigeminal ganglion were 87.78 in control group and 138.65 in experimental group. The relative fluorescence intensity of experimental group was 58% higher than that of control group. 3. In optical serial section analysis of VIP-IR cells of experimental group, most of the 9 sections showed high fluorescence intensity. At high magnification, axons of the experimental group displayed greater VIP-IR than in the control group, and the positive cells were mainly of medium size. The result indicate that number and fluorescence intensity of VIP-IR cells were increased in the mandibular part of trigeminal ganglion following pulp extirpation of mandibular molar, and it suggests that VIP could play a role in processing of nociception.

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Development of Day and Night Scope with BS Prism (BS 프리즘을 이용한 주야 조준경 개발)

  • Lee, Dong-Hee
    • Journal of Korean Ophthalmic Optics Society
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    • v.19 no.3
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    • pp.339-344
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    • 2014
  • Purpose: This study relates to the development of the day and night scope using the reflecting surface of a BS (beam splitting) prism. Methods: We have placed the LCD panel and the dot reticle generator to the top and bottom of the reflecting surface of the BS prism, and have placed a reflector, which is designed to doublet type, in the front of the BS prism. Doing so, we have developed a new type of day and night scope, which is able to image the virtual image of dot reticle from the dot reticle generator to the direction of the observer, to make the observer survey the peripheral information of the outside target by 1x magnification, and to make the observer survey the image of the LCD panel directly. Results: We could develope a new type of day and night scope, which has the function of night scope as thermal image display device at night time and the function of day scope as dot sight at day time, by letting the reflective surface of the BS prism have the role of dot sight which reflects the dot reticle and have the role of reflective optical system by which the observer surveys the night thermal image displayed in LCD panel. Conclusions: In this study, we have developed the new type of day and night scope which is able to play the role of the day or night scope selectively, combining the existing dot sight and the existing night scope by using the BS prism. By doing so, we could design and fabricate the new type of day and night scope with the BS prism which can further increase the rapidity of firing and provide more convenience in the mounting of a firearm than the detachable combination of an existing dot sight and an existing night scope.

The effect of additional etching and curing mechanism of composite resin on the dentin bond strength

  • Lee, In-Su;Son, Sung-Ae;Hur, Bock;Kwon, Yong-Hoon;Park, Jeong-Kil
    • The Journal of Advanced Prosthodontics
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    • v.5 no.4
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    • pp.479-484
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    • 2013
  • PURPOSE. The aim of this study was to evaluate the effects of additional acid etching and curing mechanism (light-curing or self-curing) of a composite resin on the dentin bond strength and compatibility of one-step self-etching adhesives. MATERIALS AND METHODS. Sixteen human permanent molars were randomly divided into eight groups according to the adhesives used (All-Bond Universal: ABU, Clearfil S3 Bond: CS3), additional acid etching (additional acid etching performed: EO, no additional acid etching performed: EX), and composite resins (Filtek Z-250: Z250, Clearfil FII New Bond: CFNB). Group 1: ABU-EO-Z250, Group 2: ABU-EO-CFNB, Group 3: ABU-EX-Z250, Group 4: ABU-EX-CFNB, Group 5: CS3-EO-Z250, Group 6: CS3-EO-CFNB, Group 7: CS3-EX-Z250, Group 8: CS3-EX-CFNB. After bonding procedures, composite resins were built up on dentin surfaces. After 24-hour water storage, the teeth were sectioned to make 10 specimens for each group. The microtensile bond strength test was performed using a microtensile testing machine. The failure mode of the fractured specimens was examined by means of an optical microscope at ${\times}20$ magnification. The data was analyzed using a one-way ANOVA and Scheffe's post-hoc test (${\alpha}$=.05). RESULTS. Additional etching groups showed significantly higher values than the no additional etching group when using All-Bond Universal. The light-cured composite resin groups showed significantly higher values than the self-cured composite resin groups in the Clearfil S3 Bond. CONCLUSION. The additional acid etching is beneficial for the dentin bond strength when using low acidic one-step self-etch adhesives, and low acidic one-step self-etch adhesives are compatible with self-cured composite resin. The acidity of the one-step self-etch adhesives is an influencing factor in terms of the dentin bonding strength and incompatibility with a self-cured composite resin.

Determination of Uranium Concentration in Solid- and Liquid-state Geological Materials by Fission Track Registration Technique and its Applicability (피션트랙 검출기법에 의한 고체 및 액체상태 지질물질의 우라늄 정량과 그 적용성)

  • Shin, Seong-Cheon
    • The Journal of the Petrological Society of Korea
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    • v.24 no.2
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    • pp.77-90
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    • 2015
  • The fission track registration techniques for accurate determinations of uranium in solid- and liquid-state geological materials were recommended and their applicability were examined. The determination of uranium can be achieved by optical counting of neutron-induced fission tracks of $^{235}U$ registered on solid-state track detectors under high magnification. In a dry registration method using powdered pellets of rocks (e.g., granite and coal) showing good uranium-affinity, it was not easy to decide an overall mean concentration over the total sample owing to track-clusters caused by frequent presence of uranium-bearing minerals. Separate scanning for homogeneous and track-clustered parts may be an alternative choice. Assuring the homogeneity over the whole sample, high reproducibilities were confirmed both from duplicate detections using mica and Lexan polycarbonate detectors and from multiple measurements at different thermal neutron fluences. The wet registration method using sealed quartz tubes is recommended to overcome the common heterogeneity in uranium concentrations of $10^1ppm$ and more. Adopting the wet registration, the uranium homogeneity was recovered below the $10^0ppm$ level and the lower detection limit was proved to reach without difficulty the $10^2ppb$ (i.e. $ng\;g^{-1}$) level.

KrF 엑시머 레이저를 이용한 웨이퍼 스텝퍼의 제작 및 성능분석

  • 이종현;최부연;김도훈;장원익;이용일;이진효
    • Korean Journal of Optics and Photonics
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    • v.4 no.1
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    • pp.15-21
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    • 1993
  • This paper describes the design and development of a KrF excimer laser stepper and discusses the detailed system parameters and characterization data obtained from the performance test. We have developed a deep UV step-and-repeat system, operating at 248 nm, by retrofitting a commercial modules such as KrF excimer laser, precision wafer stage and fused silica illumination and 5X projection optics of numerical aperture 0.42. What we have developed, to the basic structure, are wafer alignment optics, reticle alignment system, autofocusing/leveling mechanisms and environment chamber. Finally, all these subsystem were integrated under the control of microprocessor-based controllers and computer. The wafer alignment system comprises the OFF-AXIS and the TTL alignment. The OFF-AXIS alignment system was realized with two kinds of optics. One is the magnification system with the image processing technique and the other is He-Ne laser diffraction type system using the alignment grating on the wafer. 'The TTL alignment system employs a dual beam inteferometric method, which takes advantages of higher diffraction efficiency compared with other TTL type alignment systems. As the results, alignment accuracy for OFF-AXIS and TTL alignment system were obtained within 0.1 $\mu\textrm{m}$/ 3 $\sigma$ for the various substrate on the wafers. The wafer focusing and leveling system is modified version of the conventional systems using position sensitive detectors (PSD). This type of detection method showed focusing and leveling accuracies of about $\pm$ 0.1 $\mu\textrm{m}$ and $\pm$ 0.5 arcsec, respectively. From the CD measurement, we obtained 0.4 $\mu\textrm{m}$ resolution features over the full field with routine use, and 0.3 $\mu\textrm{m}$ resolution was attainable under more strict conditions.

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Self-imaging of a phase line grating and analysis of its visibility (위상형 직선격자의 자체결상과 가시도 분석)

  • 백승선;이상일;조재흥;김영란
    • Korean Journal of Optics and Photonics
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    • v.14 no.6
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    • pp.606-612
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    • 2003
  • The self-imaging effect or lensless imaging effect of a phase line grating is theoretically analyzed by using Fresnel diffraction theory, then experimentally investigated. The self-imaging distance $z_{T,p}$, that is the imaging distance being perfectly copied from the phase distribution of the phase grating to its intensity distribution with the magnification of 1X, can be uniquely defined as the (4n-3) $z_{T,a}$/4(n=positive integers), where rte is the well-known self-imaging distance of an amplitude grating. When the coherent laser beam is illuminated at the phase grating, the self-imaged images were obtained at $z_{T,p}$= $z_{T,a}$/4 and $z_{T,p}$=5 $z_{T,a}$/4 without any optics. On the other side, the phase-reversed self-imaging was obviously observed at $z_{T,p}$ = 3 $z_{T,a}$/4. The visibility of self-imaged images of a phase line grating as a function of the number of slits of the input grating was measured by the FFT(Fast Fourier Transform) results of the self-imaging images. As a result a stationary maximum visibility of V = 0.10 can be obtained from a grating with more than 15 slit pairs.n 15 slit pairs.

Development of a dual-mode energy-resolved neutron imaging detector: High spatial resolution and large field of view

  • Wenqin Yang;Jianrong Zhou;Jianqing Yang;Xingfen Jiang;Jinhao Tan;Lin Zhu;Xiaojuan Zhou;Yuanguang Xia;Li Yu;Xiuku Wang;Haiyun Teng;Jiajie Li;Yongxiang Qiu;Peixun Shen;Songlin Wang;Yadong Wei;Yushou Song;Jian Zhuang;Yubin Zhao;Junrong Zhang;Zhijia Sun;Yuanbo Chen
    • Nuclear Engineering and Technology
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    • v.56 no.7
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    • pp.2799-2805
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    • 2024
  • Energy-resolved neutron imaging is an effective way to investigate the internal structure and residual stress of materials. Different sample sizes have varying requirements for the detector's imaging field of view (FOV) and spatial resolution. Therefore, a dual-mode energy-resolved neutron imaging detector was developed, which mainly consisted of a neutron scintillator screen, a mirror, imaging lenses, and a time-stamping optical fast camera. This detector could operate in a large FOV mode or a high spatial resolution mode. To evaluate the performance of the detector, the neutron wavelength spectra and the multiple spatial resolution tests were conducted at CSNS. The results demonstrated that the detector accurately measured the neutron wavelength spectra selected by a bandwidth chopper. The best spatial resolution was about 20 ㎛ in high spatial resolution mode after event reconstruction, and a FOV of 45.0 mm × 45.0 mm was obtained in large FOV mode. The feasibility was validated to change the spatial resolution and FOV by replacing the scintillator screen and adjusting the lens magnification.