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Identification and Functional Analysis of the Chain Length Determinant Gene ste8 Involved in the Biosynthesis of Ebosin by Streptomyces sp. 139

  • Yang, Zhang;Li, Xiaohua;Qi, Xiaoqaing;Shan, Junjie;Jiang, Rong;Guo, Lianhong;Zhang, Ren;Li, Yuan
    • Journal of Microbiology and Biotechnology
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    • v.23 no.11
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    • pp.1500-1508
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    • 2013
  • Ebosin, a novel exopolysaccharide produced by Streptomyces sp. 139, has obvious antirheumatic arthritis activity in vivo, and its biosynthesis gene cluster (ste), consisting of 27 open reading frames, has been identified. This paper reports our study of the gene functionality of ste8, the predicted protein product of which is homologous to some bacterial chain length determinant Wzz proteins. For characterization of Ste8, ste8 was cloned and expressed in the mutant strain E. coli 086:H2 (${\Delta}wzz$). The functional complementation of wzz by ste8 was demonstrated by the restoration of wild-type lipopolysaccharide biosynthesis and increased levels of serum resistance of E. coli 086:H2 (${\Delta}wzz$) (pET30a-ste8). To examine the function of ste8 in ebosin biosynthesis, the gene was knocked out with a double crossover via homologous recombination. The molecular weight of the ebosin derivative EPS-8m produced by the mutant Streptomyces sp. 139 ($ste8^-$) was much lower than that of ebosin, and the binding activity of EPS-8m for IL-1R decreased significantly compared with ebosin. These results demonstrate that ste8 encodes a chain length determinant (Wzz) that functions in ebosin biosynthesis.

Experiences of High Intensity Focused Ultrasound (HIFU) Treatment in Benign Uterus Neoplasms (자궁양성종양 환자의 고강도 초음파 집속술(HIFU) 치료 경험)

  • Park, Seo A;Kim, Hye Young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.403-411
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    • 2018
  • The purpose of this study was to identify the experience of patients with uterine benign neoplasms in response to high-intensity focused ultrasound treatment. The study participants were patients who received high-intensity focused ultrasound treatment after diagnosis of benign uterine neoplasms and were contacted through purposive techniques. Five patients agreed to participate in this study. Data were collected from February, 2018 to May, 2018 at Women's hospital in D city. The interview data were analyzed by the phenomenological analysis method developed by Colaizzi using open-ended questions during in-depth interviews. Three categories emerged from the analysis using Colaizzi's method: 1) Discomfort. 2) Ambivalence. 3) Will and need. These study results contribute to a deeper understanding of the experience of patients with uterine benign neoplasms in response to high-intensity focused ultrasound treatment. Therefore, it should be developed as part of an effective nursing education program to provide evidence-based guidelines and nursing interventions that alleviate health problems in patients receiving high-intensity focused ultrasound treatment and proper nursing understanding of the development of new treatment methods for woman's health.

A study of requirements of the public library for life-long education : centering around the facilities (평생교육을 위한 공공도서관의 조건조성에 관한 연구)

  • 김남석
    • Journal of Korean Library and Information Science Society
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    • v.14
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    • pp.1-37
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    • 1987
  • The purpose of this research is to expand much more the facilities and collections of public libraries in Korea for performance of its function, for the life-long education institution. In order to accomplish the purpose of this study, I looked in to the present conditions of the facilities, collections, service facilities in public libraries and suggested a tentative plan on the basis of every kinds of statistics. The result of this thesis can be summarized as follows : 1. Now, 227, 110 populations of the effective service per library are so many that 181 public libraries in Korea have largely deficient state. To solve, a n.0, pplied for Armstrong's standards (5, 000 populations of the effective service per library, and the number of an elementary school 5, 412 elementary schools in Korea). In the former case should be establish 8, 221 public libraries, in the latter case should be established at least 5, 412 public libraries. 2. The public library in Korea is possessed of building areas of 82, 267 pyong (468 pyong per library). They are very poor state comparing with population. Therefore, the building area should be expand according to population by region. Also, the reading facilities of 88, 455 seats, 465 population of the effective service per seat, are largely insufficient of situation. And consequently they should be secured at least 205, 535 seats with standards of five seats per 1, 000 population. 3. The public libraries in Korea keep collection of 4, 517, 280 volumes. As there are 11 volumes per 100 persons, they are very deficient state in figures. Therefore, in this study, suggested Schenk's standard (1 volume per a person) and IFLA standard (2 volumes per a person). Under the circumstances of our country, however, they should be secure at least 41, 106, 993 volumes with a Schenk's standard of one volume per a person. To meet this kind of necessary conditions, there must not only be a national policy but also be a facility for every residents to utilize for educational place, and to every librarians of public libraries, they have to do their best to open their library to every citizens, when needed, with their self-conceit. Cooperating with policy-makers, users, and librarians, making every endeavors to develope Korean libraries, we can promise the hope that our public library will be progressed towards future.

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A Study on Regulatory Law for Management System of Combined Medical Device (결합 의료기기 관리제도의 규제관련 법률에 관한 연구)

  • Choi, Yong Jun;Choi, Mi Lim;Lee, Jeong Chan;Jung, Yong Gyu
    • Journal of Service Research and Studies
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    • v.4 no.2
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    • pp.1-10
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    • 2014
  • According to recent trends in technological advances and globalization, medical device industry may improve the constitution to meet worldwide medical equipment management system. Also it is holding a strand of mitigation to reach the level of international regulation. In addition, recent legislation tends are to stay limited ranges of industry regulations at least and take as open attitude for integration of new technologies combined medical devices. A greater environmental risk is not likely to work in medical technology, Combinded medical device is used as close to zero risk in most of the human body, which is classified as Class 1. Even medical device such as little or no risk in handling, it is possible to minimize the unnecessary administrative power and a waste of time to occur. For the medical device may be improving people's choices and access, medical equipment operator is expanding to include dealers, because this will be exalted to particular area of the business of the company. In this paper, we investigate the legal prerequisites for the establishment of a medical device. And propose improved regulations in topics in order to facilitate the repair and distribution markets for fair trade.

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Simulation of Mixing Behavior for Dredging Plume using Puff Model (퍼프모형을 이용한 준설플륨의 혼합거동 모의)

  • Kim, Young-Do;Park, Jae-Hyeon;Lee, Man-Soo
    • Journal of Korea Water Resources Association
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    • v.42 no.10
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    • pp.891-896
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    • 2009
  • The puff models have been developed to simulate the advection-diffusion processes of dredging suspended solids, either alone or in combination with Eulerian models. Computational efficiency and accuracy are of prime importance in designing these hybrid approaches to simulate a pollutant discharge, and we characterize two relatively simple Lagrangian techniques in this regard: forward Gaussian puff tracking (FGPT), and backward Gaussian puff tracking (BGPT). FGPT and BGPT offer dramatic savings in computational expense, but their applicability is limited by accuracy concerns in the presence of spatially variable flow or diffusivity fields or complex no-flux or open boundary conditions. For long simulations, particle and/or puff methods can transition to an Eulerian model if appropriate, since the relative computational expense of Lagrangian methods increases with time for continuous sources. Although we focus on simple Lagrangian models that are not suitable to all environmental applications, many of the implementation and computational efficiency concerns outlined herein would also be relevant to using higher order particle and puff methods to extend the near field.

Exploratory Research about Maeul Community Media Practice Grounded Theory Based Analysis about Seoul Community Media Practice (마을미디어 실천에 대한 탐색적 연구 서울마을미디어 활동에 대한 근거이론을 통한 분석)

  • Lee, Hee Rang;Kim, Hee Young
    • Korean journal of communication and information
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    • v.81
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    • pp.75-121
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    • 2017
  • Maeul Community Media is community media practice that media education combine with community activism based on the region. Maeul Community Media appeared with Seoul city's supporting policy in 2012 and is now enlarging on a national scale beyond Seoul. This study is aimed at understanding and investigating Maeul Community Media activists' practice process by using a grouned theory approach. In-depth interviews were carried out of study participants who worked on as activists based on visual and radio field since 2012. By open coding based on grounded theory, 16 categories and 59 subcategories were discovered. The outcome of axial coding showed the centeral phenomena that can be called 'sustainable desire for Maeul Community Media act'. On the condition of the instability of public support and the absence of a village channel, the strategic practices were discovered, which are diversification of participants recruitment and modes of participation, invention of space-media, seeking multilateral distribution methodologies and the collective action plans of networks to stabilize the management and to empower Maeul Community Media networks. As a result, Maeul Community Media and civil society were networked by discovering and agenda-setting pending issues. And we could find that the desire and expectation for the role as a village's public sphere were enlarging.

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A preliminary study of Oxalis corniculata L. as a new banker plant: Control efficacy against Panonychus citri (McGregor) and feeding ability of two natural enemies on Tetranychina harti (Ewing) (천적유지식물로써 괭이밥에 대한 기초연구: 괭이밥의 귤응애 밀도억제효과와 천적 2종의 괭이밥응애 포식력)

  • Oh, Chang Hak;Jin, Hye Young;Ahn, Tai Hyeon;Song, Yu Jin;Jun, Hye Jeong;Lee, Jun Seok;Ham, Eun Hye
    • Korean journal of applied entomology
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    • v.56 no.3
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    • pp.267-273
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    • 2017
  • To protect plants from damage caused by Panonychus citri (McGregor), practical methods for the use banker plants have been developed to provide a habitat and augment natural enemies of several agricultural pests. The average population density of predatory mites on an Oxalis corniculata (creeping sorrel) sod culture was significantly higher (three times) than that on normal sod culture. Furthemore, the average population density of spider mites on creeping sorrel sod culture was significantly lower (3.7 times) than when weed control was practiced. Average consumption of the eggs and females of Tetranychina harti (Ewing) by Neoseiulus californicus (McGregor) females was approximately 1.96 eggs and 1.93 females per day, respectively. Phytoseiulus persimilis AthiasHenriot females never consumed T. harti. However, total development time of T. harti from egg to adult on O. corniculata was 17.4 days, and hatchability and survival rate were 77.5% and 84.8%, respectively.

Fabrication of $TiO_2$ Electrode Containing Scattering Particles in Dye-Sensitized Solar Cells (산란 입자를 포함하는 염료감응 태양전지용 $TiO_2$ 전극 제조)

  • Lee, Jin-Hyoung;Lee, Tae-Kun;Kim, Cheol-Jin
    • Journal of the Microelectronics and Packaging Society
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    • v.18 no.2
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    • pp.57-62
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    • 2011
  • The energy conversion efficiency of DSSCs (Dye-Sensitized Solar Cells) is dependent on the powder size, the structure, and the morphology of $TiO_2$ electrode. The higher efficiency is obtained with high surface area of the nanoanatase-$TiO_2$ powder adsorbed onto a lot more of the dye. Also, the enhancement of light scattering increases the efficiency with high adsorption of the dye. Powder size, crystalline phase, and shape of $TiO_2$ obtained by hydrothermal method have 15-20 nm, anatase and round. $TiO_2$ electrode has fabricated with the mixture of scattering $TiO_2$ particle with 0.4 ${\mu}m$ in nano-sized powder. Conversion efficiency of series of DSSCs was measured with volume fraction of scattering particle. Photovoltaic characteristics of DSSCs with 10% scattering particles are 3.51 mA for Jsc (short circuit current), 0.79 V for Voc(open circuit potential), filling factor 0.619 and 6.86% for efficiency. Jsc was improved by 11% and enhancement of efficiency by 0.77% compared with that of no scattering particles. The confinement of inserted light by light scattering particles has more increase of the injection of exiton(electron-hole pair) and decrease of moving path in electron. Efficiencies of DSSCs with more than 10% for scattering particles have reduced with increasing the pore in the $TiO_2$ electrode.

Descriptive Characteristics of the Label Texts Related to Earth Science: Toward Educationally Meaningful Communication (교육적으로 유의미한 의사소통을 위한 지구과학 관련 전시 라벨의 서술 특징)

  • Kim, Chan-Jong;Park, Eun-Ji;Yoon, Sae-Yeol;Lee, Sun-Kyung
    • Journal of the Korean earth science society
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    • v.33 no.1
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    • pp.94-109
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    • 2012
  • The purpose of this study is to analyse the descriptive characteristics of the label texts related to Earth Science at a science museum and a natural history museum in Korea. The data were collected from Korean National Science Museum and Seodaemun Natural History Museum. The analysis framework was modified according to the Systemic Functional Linguistics. As a result, characteristics of the labels are 1) mostly declarative sentences, 2) appropriate amount of scientific information, and 3) mainly 'facts'. Moreover, all of the text genre are 4) 'logical expositions'. Particularly in Korean National Science Museum, the labels present 5) more scientific words among the entire terminologies and 6) more than half subjects omitted or long nominalized. Those results may imply that the labels can lead one-way communication regarding the culture of science rather than two-way. This study presents the descriptive characteristics of the label texts to make educationally meaningful communication possible by building an open structure between visitors' own culture in everyday life and the culture of science.

ZnO nanostructures for e-paper and field emission display applications

  • Sun, X.W.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2008.10a
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    • pp.993-994
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    • 2008
  • Electrochromic (EC) devices are capable of reversibly changing their optical properties upon charge injection and extraction induced by the external voltage. The characteristics of the EC device, such as low power consumption, high coloration efficiency, and memory effects under open circuit status, make them suitable for use in a variety of applications including smart windows and electronic papers. Coloration due to reduction or oxidation of redox chromophores can be used for EC devices (e-paper), but the switching time is slow (second level). Recently, with increasing demand for the low cost, lightweight flat panel display with paper-like readability (electronic paper), an EC display technology based on dye-modified $TiO_2$ nanoparticle electrode was developed. A well known organic dye molecule, viologen, was adsorbed on the surface of a mesoporous $TiO_2$ nanoparticle film to form the EC electrode. On the other hand, ZnO is a wide bandgap II-VI semiconductor which has been applied in many fields such as UV lasers, field effect transistors and transparent conductors. The bandgap of the bulk ZnO is about 3.37 eV, which is close to that of the $TiO_2$ (3.4 eV). As a traditional transparent conductor, ZnO has excellent electron transport properties, even in ZnO nanoparticle films. In the past few years, one-dimension (1D) nanostructures of ZnO have attracted extensive research interest. In particular, 1D ZnO nanowires renders much better electron transportation capability by providing a direct conduction path for electron transport and greatly reducing the number of grain boundaries. These unique advantages make ZnO nanowires a promising matrix electrode for EC dye molecule loading. ZnO nanowires grow vertically from the substrate and form a dense array (Fig. 1). The ZnO nanowires show regular hexagonal cross section and the average diameter of the ZnO nanowires is about 100 nm. The cross-section image of the ZnO nanowires array (Fig. 1) indicates that the length of the ZnO nanowires is about $6\;{\mu}m$. From one on/off cycle of the ZnO EC cell (Fig. 2). We can see that, the switching time of a ZnO nanowire electrode EC cell with an active area of $1\;{\times}\;1\;cm^2$ is 170 ms and 142 ms for coloration and bleaching, respectively. The coloration and bleaching time is faster compared to the $TiO_2$ mesoporous EC devices with both coloration and bleaching time of about 250 ms for a device with an active area of $2.5\;cm^2$. With further optimization, it is possible that the response time can reach ten(s) of millisecond, i.e. capable of displaying video. Fig. 3 shows a prototype with two different transmittance states. It can be seen that good contrast was obtained. The retention was at least a few hours for these prototypes. Being an oxide, ZnO is oxidation resistant, i.e. it is more durable for field emission cathode. ZnO nanotetropods were also applied to realize the first prototype triode field emission device, making use of scattered surface-conduction electrons for field emission (Fig. 4). The device has a high efficiency (field emitted electron to total electron ratio) of about 60%. With this high efficiency, we were able to fabricate some prototype displays (Fig. 5 showing some alphanumerical symbols). ZnO tetrapods have four legs, which guarantees that there is one leg always pointing upward, even using screen printing method to fabricate the cathode.

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