• The Korean Journal of Physiology and Pharmacology
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• v.7 no.5
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• pp.267-273
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• 2003

We have investigated the effect on inducing substate(s) of positively charged residues replaced in position 172 of the second transmembrane domain in murine inward rectifier potassium channels, formed by stable or transient transfection of Kir2.1 gene in MEL or CHO cells. Single channel recordings were obtained from either cell-attached patches or inside-out patches excised into solution containing 10 mM EDTA to rule out the effect of $Mg^{2+}$ on the channel gating. The substate(s) could be recorded with all mutants D172H, D172K and D172R. The unitary current-voltage (I-V) relation was not linear with D172H at $pH_i$ 6.3, whereas the unitary I-V relation was linear at $pH_i$ 8.0. The relative occupancy at $S_{LC}$ was increased from 0.018 at $pH_i$ 8.0 to 0.45 at $pH_i$ 5.5. In H-N dimer, that was increased from 0.016 at $pH_i$ 8.0 to 0.23 at $pH_i$ 5.5. The larger the size of the side chain or $pK_a$ with mutants (D172H, D172K and D172R), the more frequent the transitions between the fully open state and substate within an opening. The conductance of the substate also depended upon the pKa or the size of the side chain. The relative occupancy at substate $S_{LC}$ with monomer D172K (0.50) was less than that in K-H dimer (0.83). However, the relative occupancy at substate with D172R (0.79) was similar to that with R-N dimer (0.82). In the contrary to ROMK1, positive charge as well as negative charge in position 172 can induce the substate rather than block the pore in murine Kir2.1. The single channel properties of the mutant, that is, unitary I-V relation, the voltage dependence of the mean open time and relative occupancy of the substates and the increased latency to the first opening, explain the intrinsic gating observed in whole cell recordings.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.101-112
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• 2004

Chloride($Cl^-$) channels play critical roles in cell homeostasis and its specific functions such as volume regulation, differentiation, secretion, and membrane stabilization. The presence of these channels have been reported in all kinds of cells and even in frog oocytes. These essential role of $Cl^-$­ channels in cell homeostasis possibly play any role in egg homeostasis and in the early stage of development, however, there has been no report about the presence of $Cl^-$­ channel in the mammalian oocyte. This study was performed to elucidate the presence of $Cl^-$­ channels in hamster eggs. When allowing only $Cl^-$­ to pass through the channel of the egg membrane by using impermeant cation such as N-methyl-D-glucamine(NMDG), single channel currents were recorded. These channel currents showed typical long-lasted openings interrupted by rapid flickering. Mean open $time({\tau}o)$ was 43${\pm}$10.14 ms(n=9, at 50 mV). The open probability(Po) was decrease with depolarization. The current-voltage relation showed outward rectification. Outward slop conductance(32${\pm}$5.4 pS, n=22) was steeper than the inward slop conductance(10${\pm}$1.3 pS). Under the condition of symmetrical 140 mM NaCl, single channel currents were reversed at 0 mV(n=4). This reversal potential(Erev) was shifted from 0 mV at 140 mM concentration of internal NaCl(140 mM [Na+]i) to ­9.8${\pm}$0.5 mV(n=4) at 70 mM [Na+]i and 11.5${\pm}$1.9 mV at 280 mM [Na+]i(n=4) respectively, strongly suggesting that these are single $Cl^-$­ channel currents. To examine further whether this channel has pharmacological property of the $Cl^-$­ channel, specific Cl­ channel blockers, IAA-94(Indanyloxyacetic acid-94) and DIDS(4, 4'-diisothiocyan ostillben- 2-2'disulfonic acid) were applied. IAA-94 inhibited the channel current in a dose-dependent manner and revealed a rapid and flickering block. From these electrophysiological and pharmacological resluts, we found the novel $Cl^-$­ channel present in the hamster oocyte membrane. The first identification of $Cl^-$­ channel in the hamster oocyte may give a clue for the further study on the function of $Cl^-$­ channel in the fertilization and cell differentiation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.10
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• pp.4002-4014
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• 2015

ITU-R M.1842-1, as a well-known specification dedicated to maritime mobile applications, has standardized wireless transmission protocols according to the particular characteristics of a maritime communications scenario. A time division multiple access (TDMA) frame structure, along with modulation schemes to achieve a high data rate, has been described clearly in ITU-R M.1842-1. However, several specification items are still under "to be decided" status, which brings ambiguity to research works. In addition, the current version of ITU-R M.1842-1 is focused mainly on maritime transmissions in open-sea areas, where the cyclic prefix (CP) is set to zero and only 16-QAM is used in the multi-carrier (MC) system. System performance might be dramatically degraded in coastline areas due to the inter-symbol interference (ISI) caused by selective fading. This is because there is a higher probability that the signal will be reflected by obstacles in coastline areas. In this paper, we introduce the transmission resource block (TRB) dedicated to ITU-R M.1842-1 for a ship ad-hoc network (SANET), where the pilot pattern of TRB is based on the terrestrial trunked radio (TETRA). After that, we evaluated SANET performance under the maritime channel model in a coastline area. In order to avoid noise amplification and to overcome the ISI caused by selective fading, several strategies are suggested and compared in the channel estimation and equalization procedures, where the link-level simulation results finally validate our proposals.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.2
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• pp.34-43
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• 2008

An OpenLDI receiver circuit for flat-panel display systems was designed and fabricated using $1.8-{\mu}m$ high-voltage CMOS technology. Designed circuit roughly consists of DLL circuit and parallelizers, which recovers clock and parallelize data bits, respectably. It has one clock input and four data inputs. Measurement results showed that it successfully recovers clock signal from input whose frequency is $10Mhz{\sim}65Mhz$, which corresponds data rate of $70Mbps{\sim}455Mbps$ per channel, or $280Mbps{\sim}1.82Gbps$ when all of the four data channels were utilized. A commercial LCD monitor was modified into a test-bench and used for video data transmission at clock frequency of 49Mhz. In the experiment, power consumption was 19mW for core block and 82.5mW for output buffer.

• Journal of computational fluids engineering
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• v.21 no.2
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• pp.99-105
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• 2016

The present study has been carried out to analyze the flow characteristics with geometric shape and control conditions in subsea by-pass valve. The function of by-pass valve is to prevent reverse flow. In this study, the static analysis has been perform for analyzing fluid flow in open state. In order to consider the turbulent effect, the standard ${\kappa}-{\varepsilon}$ model was used. A variety of parametric studies, such as by-pass valve type or size, volume flow rate, leakage hole size, leakage hole position, block type, block shape, were performed. The pressure difference across the valve in the model broadened the flow channel cross-sectional area was greater than the base model for the same operating conditions. As the pipe diameter in the block decreases the pressure difference is greatly increased. The pressure difference according to block shape such as edge type and round was almost negligible. For the same Reynolds number the pressure difference was little changed according to the size of the valve.

• Journal of Internet of Things and Convergence
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• v.6 no.1
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• pp.97-102
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• 2020

Multi-Channel Switch ICs for IoT have integrated several solid state structure low ON-resistance bi-directional relay MOS switches with level shifter to drive high voltage and they should be independently controlled by external serialized logic control. These devices are designed for using in applications requiring high-voltage switching control by low-voltage control signals, such as medical ultra-sound imaging, ink-jet printer control, bare board open/short and leakage test system using Kelvin 4-terminal measurement method. This paper describes implementation of analog switch control block and its verification using Field programmable Gate Array (FPGA) test pattern generation. Each block has been implemented using Verilog hardware description language then simulated by Modelsim and prototyped in a FPGA board. Compare to conventional IC, The proposed architecture can be applied to fields where multiple entities need to be controlled simultaneously in the IoT environment and the proposed pattern generation method can be applied to test similar types of ICs.

• Geophysics and Geophysical Exploration
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• v.25 no.2
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• pp.59-70
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• 2022

Recent studies demonstrate that machine learning has expanded in the field of seismic interpretation. Many convolutional neural networks have been developed for seismic sequence identification, which is important for seismic interpretation. However, expense and time limitations indicate that there is insufficient data available to provide a sufficient dataset to train supervised machine learning programs to identify seismic sequences. In this study, patch division and data augmentation are applied to mitigate this lack of data. Furthermore, to obtain spatial information that could be lost during patch division, an artificial channel is added to the original data to indicate depth. Seismic sequence identification is performed using a U-Net network and the Netherlands F3 block dataset from the dGB Open Seismic Repository, which offers datasets for machine learning, and the predicted results are evaluated. The results show that patch-based U-Net seismic sequence identification is improved by data augmentation and the addition of an artificial channel.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.43 no.3 s.345
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• pp.1-9
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• 2006

In this paper, we propose a MIMO-OFDMA (Multi Input Multi Output-Orthogonal Frequency Division Multiple Access) system based on beamforming for performance improvement of downlink real-time traffic transmission in harsh channel conditions with low CIR (Carrier-to-Interference Ratio). In the proposed system, we first consider the M-GTA-SBA (Modified-Grouped Transmit Antenna-Simple Bit Allocation) using effective CSI (Channel State Information) calculation procedure based on spatial resource grouping, which is adequate for the combination of MRT (Maximum Ratio Transmission) in the transmitter and MRC (Maximum Ratio Combining) in the receiver. In addition, to reduce feedback information for the beamforming, we also apply QEGT (Quantized Equal Gain Transmission) based on quantization of amplitudes and phases of beam weights. Furthermore, considering multi-user environments, we propose the P-SRA (Proposed-Simple Resource Allocation) algorithm for fair and efficient resource allocation. Simulation results reveal that the proposed MIMO-OFDMA system achieves significant improvement of spectral efficiency in low CRI region as compared to a typical open-loop MIMO-OFDMA system using pseudo-orthogonal space time block code and H-ARQ IR (Hybrid-Automatic Repeat Request Incremental Redundancy).

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.19-29
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• 1996

The neurological manifestations of AIDS include dementia, encountered even in the absence of opportunistic superinfection or malignancy. The AIDS Dementia Complex appears to be associated with several neuropathological abnormalities, including astrogliosis and neuronal injury or loss. How can HIV-1 result in neuronal damage if neurons themselves are only rarely, if ever, infected by the vitus\ulcorner In vitro experiments from several different laboratiories have lent support to the existence of HIV- and immune-related toxins. In one recently defined pathway to neuronal injury, HIV-infected macrophages/microglia as well as macrophages activated by HIV-1 envelope protein gp120 appear to secrete excitants/neurotoxins. These substances may include arachidonic acid, platelet-activating factor, free radicals (NO - and O$_2$), glutamate, quinolinate, cysteine, cytokines (TNF-${\alpha}$, IL1-B, IL-6), and as yet unidentified factors emanating from stimulated macrophages and possibly reactive astrocytes. A final common pathway for newonal suscepubility appears to be operative, similar to that observed in stroke, trauma, epilepsy, and several neurodegenerative diseases, including Huntington's disease, Parkinson's disease, and amyotrophic lateral sclerosis. This mechanism involves excessive activation of N-methyl-D-aspartate (NMDA) receptor-operated channels, with resultant excessive influx of Ca$\^$2+/ leading to neuronal damage, and thus offers hope for future pharmacological intervention. This chapter reviews two clinically-tolerated NMDA antagonists, memantine and nitroglycerin; (ⅰ) Memantine is an open-channel blocker of the NMDA-associated ion channel and a close congener of the anti-viral and anti-parkinsonian drug amantadine. Memantine blocks the effects of escalating levels of excitotoxins to a greater degree than lower (piysiological) levels of these excitatory amino acids, thus sparing to some extent normal neuronal function. (ⅱ) Niuoglycerin acts at a redox modulatory site of the NMDA receptor/complex to downregulate its activity. The neuroprotective action of nitroglycerin at this site is mediated by n chemical species related to nitric oxide, but in a higher oxidation state, resulting in transfer of an NO group to a critical cysteine on the NMDA receptor. Because of the clinical safety of these drugs, they have the potential for trials in humans. As the structural basis for redox modulation is further elucidated, it may become possible to design even better redox reactive reagents of chinical value. To this end, redox modulatory sites of NMDA receptors have begun to be characterized at a molecular level using site-directed mutagenesis of recombinant subunits (NMDAR1, NMDAR2A-D). Two types of redox modulation can be distinguished. The first type gives rise to a persistent change in the functional activity of the receptor, and we have identified two cysteine residues on the NMDARI subunit (#744 and #798) that are responsible for this action. A second site, presumably also a cysteine(s) because <1 mM N-ethylmaleimide can block its effect in native neurons, underlies the other, more transient redox action. It appears to be at this, as yet unidentified, site on the NMDA receptor that the NO group acts, at least in recombinant receptors.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.7 s.361
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• pp.59-69
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• 2007

In this paper, we propose a MIMO-OFDMA (Multi Input Multi Output-Orthogonal Frequency Division Multiple Access) system based on Grassmannian beamforming for performance improvement of downlink real-time traffic transmission in harsh channel conditions with low CIR (Carrier-to-Interference Ratio). In the proposed system to reduce feedback information for the beamforming, we also apply Grassmannian Beamforming. Furthermore, we propose antenna selection scheme which performs the beamforming with more useful transmit antennas. In the proposed system, the optimal combination of transmit antennas with maximum MRT (Maximum Ratio Transmission) beamforming gain, is selected. Simulation results reveal that the proposed MIMO-OFDMA system achieves significant improvement of spectral efficiency in low CIR region as compared to a typical open-loop MIMO-OFDMA system using pseudo-orthogonal space time block code.