• Plant Resources
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• 제8권1호
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• pp.1-8
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• 2005

A type 3 metallothionein cDNA (PMT3a) from ozone-treated Populus alba${\times}$Populus glandulosa cDNA library has been isolated and characterized. A PMT3a cDNA is 459 nucleotides long and has an open reading frame of 201 bp with a deduced amino acid sequence of 66 residues (pI 4.94). The deduced amino acid sequence of PMT3a matched to the previously reported metallothionein genes. The deduced amino acid sequence of PMT3a showed the $86\%$ identity with P. balsamifera ${\times}$P. deltoides. Expression of PMT3a by the RT-PCR was increased 60 min than 30 min after drought treatment. The ozone treated poplar increased at 30 min in the early time and then decreased at 60 min.

• Archives of Pharmacal Research
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• 제24권4호
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• pp.316-322
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• 2001

Arylsulfate sulfotransferase (ASST) transfers a sulfate group from a phenolic sulfate ester to a phenolic acceptor substrate. In the present study, the gene encoding ASST was cloned from a genomic library copy of Citrobacter freundii, subcloned into the vector pGEM3Zf(-) and sequenced. Sequencing revealed two contiguous open reading frames (ORF1 and ORF2) on the same strand and based on amino acid sequence homologyl they were designated as astA and dsbA, respectively. The amino acid sequence of astA deduced from C. freundii was highly similar to that of the Salmonella typhimurium, Enterobacter amnigenus, Klebsiella, Pseudomonas putida, and Campylobacter jejuni, encoded by the astA genes. However, the ASST activity assay revealed different acceptor specificities. Using p-nitrophenyl sulfate (PNS) as a donor substrate, $\alpha$-naphthol was found to be the best acceptor substrate, followed by phenol, resorcinol, p-acetaminophen, tyramine and tyrosine.

• Molecules and Cells
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• 제38권2호
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• pp.171-179
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• 2015

Prostatic acid phosphatase (PAP) expression increases proportionally with prostate cancer progression, making it useful in prognosticating intermediate to high-risk prostate cancers. A novel ligand that can specifically bind to PAP would be very helpful for guiding prostate cancer therapy. RNA aptamers bind to target molecules with high specificity and have key advantages such as low immunogenicity and easy synthesis. Here, human PAP-specific aptamers were screened from a 2'-fluoropyrimidine (FY)-modified RNA library by SELEX. The candidate aptamer families were identified within six rounds followed by analysis of their sequences and PAP-specific binding. A gel shift assay was used to identify PAP binding aptamers and the 6N aptamer specifically bound to PAP with a Kd value of 118 nM. RT-PCR and fluorescence labeling analyses revealed that the 6N aptamer bound to PAP-positive mammalian cells, such as PC-3 and LNCaP. IMR-90 negative control cells did not bind the 6N aptamer. Systematic minimization analyses revealed that 50 nucleotide sequences and their two hairpin structures in the 6N 2'-FY RNA aptamer were equally important for PAP binding. Renewed interest in PAP combined with the versatility of RNA aptamers, including conjugation of anti-cancer drugs and nano-imaging probes, could open up a new route for early theragnosis of prostate cancer.

• 가정∙방문간호학회지
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• 제25권2호
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• pp.127-138
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• 2018

Purpose: This study aimed to provide an overview of the present status of start-up literacy. Methods: The study selected literature from KoreaMed, RISS (Research Information Sharing Service), KISS (Korean studies Information Service System), KISTI (Korea Institute of Science and Technology Information), Ovid-MEDLINE (Medical Literature Analysis and Retrieval System Online), Embase, and Cochrane Library DB. Start-up curricula were collected from the homepage of nursing schools in Korea and the USA. Their contents were searched using Massive Open Online Course (MOOC) platforms; KOCW, K-MOOC, and Coursera. Results: Fifteen articles were selected through a systematic literature review. The main themes of nursing start-up were "driver," "barrier," "required competency," and "importance of education." The courses contained business planning, finance, marketing, leadership, and entrepreneurship. The main contents of MOOCs were similar. Conclusion: The results indicate the necessity to develop multi-scope nursing start-up education programs. Nursing start-up models integrated with appropriate business knowledge and skills in health care settings are needed. This study can be used as a guideline to design start-up education programs in nursing.

• 한국위성정보통신학회논문지
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• 제7권1호
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• pp.48-52
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• 2012

스마트폰의 급속한 보급 확산에 따라 모바일 기기에서 웹 브라우저는 주요한 기능으로 자리 잡고 있으며 사용자는 모바일 기기에서 PC 수준의 고속화된 성능을 기대하는 현실이다. 웹키트(WebKit)는 구글의 안드로이드(Android) 플랫폼에 사용되고 있는 웹 브라우저를 만드는 데 기반을 제공하는 오픈 소스 응용 프로그램 프레임워크이다. 본 논문에서는 WebKit 라이브러리의 연산의 병렬처리를 통한 성능 향상 기법을 제안하였다. 제안된 병렬처리 기법은 JPEG 라이브러리에 적용되었으며, 성능 검증은 PC 기반의 실험을 통하여 이기종 컴퓨팅 기반의 모바일 임베디드 시스템 환경에서의 예측 방법을 사용하였다. 실험 결과는 제안된 병렬화 기법이 이기종 컴퓨팅 환경의 모바일 임베디드 기기로의 적용을 통한 성능 향상 가능성을 보여주었다.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제21권8호
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• pp.561-566
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• 2015

하둡은 오픈소스 기반의 분산 데이터 처리 프레임워크로서 과학 및 상용 분야에서 널리 사용되고 있는데 최근에 대규모 데이터의 실시간 처리 및 분석을 위해 고성능 컴퓨팅(HPC) 기술을 활용하여 하둡을 고성능화하기 위한 연구가 시도되고 있다. 본 논문에서는 하둡의 기본 파일시스템 구현인 하둡 분산파일시스템(HDFS)을 고성능 병렬 분산파일시스템인 러스터 파일시스템으로 대체하여 사용할 수 있도록 하둡 파일시스템 라이브러리를 확장하여 구현하였고 하둡이 제공하는 표준 벤치마크 도구를 사용하여 성능을 분석하였다. 실험 결과 러스터 파일시스템 기반으로 하둡 맵리듀스 응용을 수행하는 경우에 2-13배의 성능 향상이 있음을 확인할 수 있었다.

• Journal of Microbiology
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• 제41권4호
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• pp.295-299
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• 2003

Adenovirus (Ad) precursor to the terminal protein (pTP) plays an essential roles in the viral DNA replication. Ad pTP serves as a primer for the synthesis of a new DNA strand during the initiation step of replication. In addition, Ad pTP forms organized spherical replication foci on the nuclear matrix (NM) and anchors the viral genome to the NM. Here we identified the interferon inducible gene product 1-8D (Inid) as a pTP binding protein by using a two-hybrid screen of a HeLa cDNA library. Of the clones obtained in this assay, nine were identical to the Inid, a 13-kDa polypeptide that shares homology with genes 1-8U and Leu-13/9-27, most of which have little known functions. The entire open reading frame (ORF) of Inid was cloned into the tetracycline inducible expression vector in order to determine the biological functions related with adenoviral infection. When Inid was introduced to the cells along with adenoviruses, fifty to sixty percent of Ad-infected cells expressing Inid had rounded morphology, which was suggestive of apoptosis. Results from the terminal deoxynucleotidyl transferase (TdT) and DNA fragmentation assays confirmed that Inid induces apoptosis in Ad-infected or in uninfected cells. The Inid binding to pTP may target the cell for apoptotic destruction as a host defense mechanism against the viral infection.

• Journal of Microbiology
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• 제38권4호
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• pp.230-238
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• 2000

Class III chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class III chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5'flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• 대한원격탐사학회지
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• 제22권3호
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• pp.221-228
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• 2006

In this study, an integrated 3D modeling and rendering system dealing with 3D urban landscape features such as terrain, building, road and user-defined geometric ones was designed and implemented using $OPENGL\;{|}\;ES$ (Embedded System) API for mobile devices of PDA. In this system, the authoring functions are composed of several parts handling urban landscape features: vertex-based geometry modeling, editing and manipulating 3D landscape objects, generating geometrically complex type features with attributes for 3D objects, and texture mapping of complex types using image library. It is a kind of feature-based system, linked with 3D geo-based spatial feature attributes. As for the rendering process, some functions are provided: optimizing of integrated multiple 3D landscape objects, and rendering of texture-mapped 3D landscape objects. By the active-synchronized process among desktop system, OPENGL-based 3D visualization system, and mobile system, it is possible to transfer and disseminate 3D feature models through both systems. In this mobile 3D urban processing system, the main graphical user interface and core components is implemented under EVC 4.0 MFC and tested at PDA running on windows mobile and Pocket Pc. It is expected that the mobile 3D geo-spatial information systems supporting registration, modeling, and rendering functions can be effectively utilized for real time 3D urban planning and 3D mobile mapping on the site.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2018년도 학술발표회
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• pp.147-147
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• 2018

In this article, we use an open source software library: TensorFlow, developed for the purposes of conducting very complex machine learning and deep neural network applications. However, the system is general enough to be applicable in a wide variety of other domains as well. The proposed model based on a deep neural network model, LSTM (Long Short-Term Memory) to predict the river water level at Okcheon Station of the Guem River without utilization of rainfall - forecast information. For LSTM modeling, the input data is hourly water level data for 15 years from 2002 to 2016 at 4 stations includes 3 upstream stations (Sutong, Hotan, and Songcheon) and the forecasting-target station (Okcheon). The data are subdivided into three purposes: a training data set, a testing data set and a validation data set. The model was formulated to predict Okcheon Station water level for many cases from 3 hours to 12 hours of lead time. Although the model does not require many input data such as climate, geography, land-use for rainfall-runoff simulation, the prediction is very stable and reliable up to 9 hours of lead time with the Nash - Sutcliffe efficiency (NSE) is higher than 0.90 and the root mean square error (RMSE) is lower than 12cm. The result indicated that the method is able to produce the river water level time series and be applicable to the practical flood forecasting instead of hydrologic modeling approaches.