• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1581-1590
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• 2018

Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1759-1767
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• 2016

Background: Uterine sarcoma is a group of rare gynecologic tumors with various natures, and different lines of treatment. Most have a poor treatment outcome. This study targeted clinical characteristics, treatment, overall survival (OS), progression-free survival (PFS), and prognostic factors in uterine sarcoma patients in one tertiary center for cancer care. Materials and Methods: Uterine sarcoma patients who were treated at the Department of Obstetrics and Gynecology, Faculty of Medicine Vajira Hospital between January 1994 and December 2014 were identified. Clinico-pathological data were analyzed. Prognostic outcomes were examined by Kaplan-Meier curves and Cox regression analysis. Results: We identified 46 uterine sarcoma patients: 25 carcinosarcoma (CS) (54.3%), 15 leiomyosarcoma (LMS) (32.6%), and 6 undifferentiated uterine sarcoma (UUS) (13.1%) cases. Mean age was $54.0{\pm}11.9years$ (range 25-82 years). Abnormal uterine bleeding was the most common presenting symptom (63.0%). Among 33 patients (71.7%) who had pre-operative tissue collected, diagnosis of malignancy was correct in 29 (87.9%). All patients received primary surgery and retroperitoneal lymph nodes were resected in 34 (73.9%). After surgery, 5 (10.9%) had gross residual tumors. Stage I disease was most commonly found (56.5%). Adjuvant treatment was given to 27 (58.7%), most commonly chemotherapy. After a median follow-up of 16.0 months (range 0.8-187.4 months), recurrence was encountered in 22 patients (47.8%). Median time to recurrence was 5.8 months (range1.0-105.5 months). Distant metastasis was more common than local or locoregional failure. The 2-year PFS was 45.2% (95% confidence interval [CI], 30.6%-59.7%) and the 2-year OS was 48.3% (95% CI, 33.3%-60.7%). Multivariable analyses found residual disease after surgery as a significant factor only for PFS. Conclusions: Uterine sarcoma is a rare tumor entity. Even with multimodalities of treatment, the prognosis is still poor. Successful cytoreductive surgery is a key factor for a good survival outcome.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1901-1906
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• 2012

Background: The discovery that microRNA (miRNA) regulates metastasis provide a principal molecular basis for tumor heterogeneity. A characteristic of solid tumors is their heterogenous distribution of blood vessels, with significant hypoxia occurring in regions (centers of tumor) of low blood flow. It is necessary to discover the mechanism of breast cancer metastasis in relation to the fact that there is a differential distribution of crucial microRNA in tumors from centers to edges. Methods: Breast tissues from 48 patients (32 patients with breast cancer) were classified into the high invasive and metastatic group (HIMG), low invasive and metastatic group (LIMG), and normal group. Samples were collected from both the centers and edges of all tumors. The first six specimens were detected by microRNA array, and the second ten specimens were detected by real-time qRT-PCR and Western blot analyses. Correlation analysis was performed between the miRNAs and target proteins. Results: The relative content of miR-20a and miR-20b was lower in the center of the tumor than at the edge in the LIMG, lower at the edge of the tumor than in the center in the HIMG, and lower in breast cancer tissues than in normal tissues. VEGF-A and HIF-1alpha mRNA levels were higher in the HIMG than in the LIMG, and levels were higher in both groups than in the normal group; there was no difference in mRNA levels between the edge and center of the tumor. VEGF-A and HIF-1alpha protein levels were higher in the HIMG than in the LIMG, and protein levels in both groups were higher than in the normal group; there was a significant difference in protein expression between the edge and center of the tumor. Correlation analysis showed that the key miRNAs (miR-20a and miR-20b) negatively correlated with the target proteins (VEGF-A and HIF-1alpha). Conclusions: Our data suggest that miR-20a and miR-20b are differentially distributed in breast cancer, while VEGF-A and HIF-1alpha mRNA had coincident distributions, and VEGF-A and HIF-1alpha proteins had uneven and opposing distributions to the miRNAs. It appears that one of the most important facets underlying metastatic heterogeneity is the differential distribution of miR-20a and miR-20b and their regulation of target proteins.

• 벤처창업연구
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• 제13권4호
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• pp.169-181
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• 2018

지속적으로 심화되고 있는 글로벌 경쟁 환경 하에서 많은 기업들은 공유가치 창출 (CSV, Creating Shared Value)을 통해 사회혁신 분야에서 새로운 사업 기회를 모색하고 있다. 사회혁신을 추구함에 있어 해결하고자 하는 문제를 명확히 도출하고, 문제의 원인을 파악하는 것이 사회혁신의 핵심 출발점이다. 수많은 문제해결 방법론 중에 최근 다양한 분야에서 가장 많은 관심을 받는 것은 디자인 씽킹 (Design Thinking)이다. 디자인 씽킹은 인간의 필요에 공감하고 대중도 인식하지 못하는 잠재적 욕구를 발굴하여 해결하는 창의적 문제해결 방법론으로 비즈니스 혁신도구로도 사용되지만 사회 문제를 해결하는 사회혁신을 위한 도구로서 활발히 이용되고 있다. 그러나 디자인 씽킹 프로젝트를 수행하는 많은 주체들이 겪는 어려움 중의 하나가 관찰 데이터의 효율적 분석이 어렵다는 것이다. 오프라인 방식으로만 데이터를 분석할 경우 대량의 데이터를 처리하는데 시간이 오래 걸리며, 비정형데이터의 경우 처리가 어려워 관찰을 통해 어렵게 수집한 데이터로부터 문제점을 발견하는데 어려움을 호소한다. 이에 본 연구는 디자인 씽킹 프로세스의 한계점을 보완하고자 사회혁신을 위한 디자인 씽킹 프로젝트의 관찰단계에서 수집한 데이터 분석이 보다 과학적으로 이루어질 수 있도록 질적 자료 분석과 양적 자료 분석 방법을 균형 있게 사용하는 통합방법론을 제시하고자 한다.

• 한국통신학회논문지
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• 제37C권11호
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• pp.1094-1103
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• 2012

최근 국방 소프트웨어 산업은 다양한 무기체계 사업을 통해 모델링 및 시뮬레이션 기술을 적용한 시뮬레이션 시스템 개발을 늘리고 있으며, 특히 이기종 시뮬레이터 간 이식성 및 상호 연동성 확보를 위해서 분산 시뮬레이션 표준 프레임워크인 HLA(High Level Architecture)의 적용을 규정하고 있다. HLA는 분산 환경에서 시뮬레이터간 데이터 교환 및 순서화를 제공하기 위한 서비스를 정의하며, HLA 규칙, Federate 인터페이스 표준 그리고 객체 모델 템플릿의 주요 컴포넌트로 구성된다. RTI(Run-Time Infrastructure)는 Federate 인터페이스 표준을 구현한 소프트웨어로써 Federation 환경에 참여 중인 Federate들이 정보를 교환할 수 있도록 기능을 제공한다. RTI 기술은 워 게임, 가상 시뮬레이션, 훈련 및 무기체계 소프트웨어 연동과 같은 다양한 분야에서 사용되고 있다. 하지만 국내에서 개발된 사례가 없어 모두 외산 제품에 의존하고 있는 실정이다. 본 논문은 국내에서 개발된 IEEE1516.1-2000 표준의 RTI 구현을 소개한다. 특히, Federate 간 데이터 교환 성능을 높이기 위해서 서버-클라이언트 방식과 단대단 방식을 혼합한 하이브리드 데이터 통신 방식의 적용 방법을 기술하고, 상용 RTI와 데이터 처리율 및 네트워크 지연 시간의 비교를 통해 성능이 개선되었음을 보인다.

• 유통과학연구
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• 제13권5호
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• pp.53-60
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• 2015

Purpose - The purpose of this paper is to shed light on the importance of the slope and curvature of the volatility curve implied in option prices in the KOSPI 200 options index. A number of studies examine the implied volatility curve, however, these usually focus on cross-sectional characteristics such as the volatility smile. Contrary to previous studies, we focus on time-series characteristics; we investigate correlation dynamics among slope, curvature, and level of the implied volatility curve to capture market information embodied therein. Our study may provide useful implications for investors to utilize current market expectations in managing portfolios dynamically and efficiently. Research design, data, and methodology - For our empirical purpose, we gathered daily KOSPI200 index option prices executed at 2:50 pm in the Korean Exchange distribution market during the period of January 2, 2004 and January 31, 2012. In order to measure slope and curvature of the volatility curve, we use approximated delta distance; the slope is defined as the difference of implied volatilities between 15 delta call options and 15 delta put options; the curvature is defined as the difference between out-of-the-money (OTM) options and at-the-money (ATM) options. We use generalized method of moments (GMM) and the seemingly unrelated regression (SUR) method to verify correlations among level, slope, and curvature of the implied volatility curve with statistical support. Results - We find that slope as well as curvature is positively correlated with volatility level, implying that put option prices increase in a downward market. Further, we find that curvature and slope are positively correlated; however, the relation is weakened at deep moneyness. The results lead us to examine whether slope decreases monotonically as the delta increases, and it is verified with statistical significance that the deeper the moneyness, the lower the slope. It enables us to infer that when volatility surges above a certain level due to any tail risk, investors would rather take long positions in OTM call options, expecting market recovery in the near future. Conclusions - Our results are the evidence of the investor's increasing hedging demand for put options when downside market risks are expected. Adding to this, the slope and curvature of the volatility curve may provide important information regarding the timing of market recovery from a nosedive. For financial product distributors, using the dynamic relation among the three key indicators of the implied volatility curve might be helpful in enhancing profit and gaining trust and loyalty. However, it should be noted that our implications are limited since we do not provide rigorous evidence for the predictability power of volatility curves. Meaning, we need to verify whether the slope and curvature of the volatility curve have statistical significance in predicting the market trough. As one of the verifications, for instance, the performance of trading strategy based on information of slope and curvature could be tested. We reserve this for the future research.

• 시큐리티연구
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• 제39호
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• pp.63-93
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• 2014

시민은 지역사회의 안전주체로서 비중이 점차 증가하고, 이들 활동을 지원하기 위한 입법화가 진행되고 있으나, 이에 대한 체계적이고 구체적인 활용방안은 미흡한 실정이다. 이 연구의 목적은 시민자율방범대의 현장 활용도를 증대시키기 위한 교육프로그램 모형을 개발하는 것이다. 연구방법은 문헌조사를 통해 시민자율방범에 대한 이해와 현황을 분석하고 교육프로그램 개발을 위한 이론적 기초를 살펴보고, 사례분석으로 기히 운영되었던 자율방범대 교육 프로그램의 운영성과를 분석하여 도출된 문제점을 바탕으로 '현장 활용도 증대를 위한 시민자율방범 교육프로그램 모형'을 제안하였다. 연구결과는 첫째, 시민자율방범대 운영사례에서 파악된 학습자들의 요구는 현장에서 활용할 수 있는 교육내용과 충분한 교육시간이 필요하다는 것이다. 둘째, 교육프로그램 모형의 목표인 '현장 활용도 증대를 위한 교육'을 달성하기 위한 하위목표는 '수요자 중심, 현장 중심, 역량강화'로 설정하고, 추진전략은 기획-설계-실행-평가의 4단계로 구성되어야 한다. 그리고 각 단계별 추진전략은 교육의 준비에서 진행까지 필요한 세부전략이 고려되어야 한다. 셋째, 이 교육프로그램은 학습자의 수준에 따라 기초-중간-전문 과정으로 분류하고, 각 과정별로 수준을 고려한 이론 및 실무중심의 교과목이 균형적으로 편성되어야 한다.

• 대한교통학회지
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• 제25권5호
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• pp.101-109
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• 2007

고속도로는 폐쇄식과 개방식 영업체계 두 개의 방식으로 운영되고 있다. 2001년을 기점으로 하여 대부분 폐쇄식으로 전환되었으며, 수도권을 제외하고는 거의 폐쇄식으로 운영되고 있다. 이는 주민들이 개방식에 대한 요금의 불합리성을 민원으로 끊임없이 제기하기 때문이다. 이러한 민원을 발생시키는 개방식 영업체계는 시설비/운영비가 적게 소요되며, 간선기능의 효과 등의 장점이 있어 도시부간선도로와 같이 단거리 통행량이 많은 노선에 적합하나 영업소 통과차량에만 요금이 부과되므로 주행거리와 상관없이 동일 요금이 적용 된다는 점과 영업소를 통과하지 않는 일부 구간에 무료차량을 발생하므로 불공정 문제가 야기 되고 있다. 개방식으로 운영되고 있는 서울외곽순환고속도로의 경우 1992년 개통이후 현재까지 개방식 구간을 이용한 일일 교통량의 연평균 증가율이 10%를 넘고 있다. 이러한 이용대수의 증가에 따라 일부 무료이용 구간에서 교통체증이 발생하고 있어 무료이용자와 유료이용자 간의 형평성 문제가 대두되고 있다. 또한 서울외곽순환고속도로의 경우 민자고속도로인 서울외곽순환고속도로(일산${\sim}$퇴계원)가 개통됨에 따라 민자고속도로와 한국도로공사 관할 서울외곽순환고속도로 상호간의 영업체계상의 일관성이 없는 문제가 발생한다. 따라서 본 연구는 개방식 고속도로 무료이용구간의 통행특성 분석과 폐쇄식으로의 전환시 영향을 분석하였다.

• 대한핵의학회지
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• 제29권1호
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• pp.48-53
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• 1995

Rb-82 dynamic PET과 이중적분법에 의한 국소 심근 혈류측정 연구를 시행하고자 실험 개를 이용한 심근 혈전증 모델에서 좌심실 입력함수에 의한 정상 및 관류결손 심근에서의 혈류를 측정하였다. 이중적분법이 선형회귀모델에 의한 혈류측정방법에 비하여 실현이 간단하고 심근내 혈류가 선형적인 가정을 배제할 수 있어 더욱 정확한 방법이 될 수 있음을 확인하였다.

• 자원환경지질
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• 제19권1호
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• pp.1-18
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• 1986

A group of 16 $Zn+Pb{\pm}Ag$ deposits distributed in the Pyeongchang-Jucheon area, Kangwon-do, South Korea, were semi-regionally investigated. These deposits are contact metasomatic and/or hydrothermal replacement types hosted in the carbonate-dominated Cambrian Machari Formation and Ordovician Ibtanri Formation, and also in the carbonate interbeds of the Precambrian argillic metasediments. Comparing some key aspects of the individual deposits, it is found that the ore deposits hosted in the Machari and Ibtanri Formations are mostly of steeply-dipping chimneys with or without skarn minerals and are rich in Ag and Pb>Zn in metal grade whereas those occuring in the carbonate interbeds of the Precambrian argillic metasediments are gently-dipping conformable lenticular orebodies mostly with skarn minerals and are generally poor in Ag and Zn>Pb. The skarn mineralization in the area appears to have occurred during the lower Cretaceous (118.7Ma) to mid-Cretaceous (107.8Ma) time assumed from the K-Ar dates of the Dowon and Pyeongchang granites which are closely associated with the skarn ore deposits. The Rb/Ba/Sr ratios of these granites indicate that they are of strongly differentiated anomalous granites, and the Nb vs. Y and Rb vs. Y+Nb plots fall on the field of volcanic arc setting. The contact aureoles are zoned, giving the sequence in order of increasing distance from igneous contact: garnet-wollastonite, granet-wollastonite-clinopyroxene and garnet-clinopyroxene in such as the Pyeongchang and Yeonwol 114 areas. Electron microprobe analyses reveal that garnets and clinopyroxenes are generally low in Fe and Mn. Garnets are grossular to intermediate grandite except for those from the Ogryong exoskarn which are richer in andradite, pyrope and spessartine fractions. This indicates that the oxidation state of skarn-forming environment at Ogryong was higher than at the other deposits. Clinopyroxenes are mostly salitic except for those from the Ogryong exoskarn which involve considerable amounts of hedenbergite and johansenite fractions. The ${\delta}^{18}O$ value of Jurassic biotite granite at Ogryong is higher (+10.21‰) than that of Cretaceous one at Chodun (+8.41‰). The ${\delta}^{13}C$ values of carbonate rocks range from -0.89‰ to 0.68‰ and the ${\delta}^{18}O$ values range from +11.91‰ to + 19.34‰ indicating that these carbonate rocks are of marine origin. However, the ${\delta}^{13}C$ values of skarn calcite and vein calcite are -4.80‰ and -12.92‰, and the ${\delta}^{18}O$ values are +5.56‰ and +10.32‰, respectively, indicating that these calcites are of hydrothermal origin. The ${\delta}^{34}S$ values of sulfide minerals range from +4.4‰ to +8.7‰ suggesting that the sulfurs are of magmatic origin.