• 생명과학회지
• /
• 제21권1호
• /
• pp.134-140
• /
• 2011

대전일원의 유류오염 지역의 토양으로부터 원유를 단일 탄소원으로 이용하는 총 145균주를 순수분리 하였고, 이중 생물 계면활성제 생성능이 가장 우수한 한 균주를 최종 선별하여 형태 및 생리 생화학적 특성을 조사하고 16S rRNA 염기서열을 분석을 통하여 동정한 결과 Pseudomonas sp.로 확인되어 Pseudomonas sp. Z1이라 명명하였다. 최종 선별된 Pseudomonas sp. Z1은 클로람페니콜과 암피실린 등의 항생제와 리튬, 망간, 바륨 등의 중금속에 대해 강한 내성을 갖고 있었고, 최적 온도와 pH는 각각 $30^{\circ}C$와 pH 6.0-7.0으로 확인되었다. Pseudomonas sp. Z1이 생성하는 생물 계면활성제는 배양 10시간 이후부터 배양액의 표면장력이 급격히 감소해, 배양 21시간 후에 최대 28 dyne/cm까지 감소되었고, 2% 이상의 NaCl을 첨가한 경우 배양액의 생물계면활성제의 활성이 감소하였다.

• Molecular & Cellular Toxicology
• /
• 제1권3호
• /
• pp.189-195
• /
• 2005

Industrial development has increase consumption of crude oil and environmental pollution. A large number of microbial lipolytic enzymes have been identified and characterized to date. To development for a new lipase with catalytic activity in degradation of crude oil as a microbial enzyme, Acinetobactor sp. B2 was isolated from soil samples that were contaminated with oil in Daejon area. Acinetobactor sp. B2 showed high resistance up to 10 mg/mL unit to heavy metals such as Ba, Li, Al, Cr, Pb and Mn. Optimal growth condition of Acinetobactor sp. B2 was confirmed $30^{\circ}C$. Lipase was purified from the supernatant by Acinetobactor sp. B2. Its molecular mass was determined to the 60 kDa and the optimal activity was shown at $40^{\circ}C$ and pH 10. The activation energies for the hydrolysis of p-nitrophenyl palmitate were determined to be 2.7 kcal/mol in the temperature range 4 to $37^{\circ}C$. The enzyme was unstable at temperatures higher than $60^{\circ}C$. The Michaelis constant $(K_{m})\;and\;V_{max}$ for p-nitrophenyl palmitate were $21.8{\mu}M\;and\;270.3{\mu}M\;min^{-1}mg\;of\;protein^{-1}$, respectively. The enzyme was strongly inhibited by $Cd{2+},\;Co^{2+},\;Fe^{2+},\;Hg^{2+},\;EDTA$, 2-Mercaptoethalol. From these results, we suggested that lipase purified from Acinetobactor sp. B2 should be able to be used as a new enzyme for degradation of crude oil, one of the environmental contaminants.

• 복식문화연구
• /
• 제14권5호
• /
• pp.828-839
• /
• 2006

These days, by the accelerated developments of science and industries and the menaces of war and terror, humans have come to have instinct to recur and nostalgia to nature and thereby many art works and designs with natural objectives have begun to be produced. Also the design in 21st century requires creative products based on cultural speciality of the countries. As the examples, we may easily find the images of the countries from visual expressions or products when we get in touch with the products of so called advanced countries in design i. e. U.S.A. Japan, Germany, France, Italy, countries of Scandinavian peninsular. This study, in such stream of the times, was to express our culture's own originality on clothing design with butterfly that is one of natural thing as the material but limited the boundary to realistic butterflies in folk drawings of the times of Chosun. As for the technique of expression, used digital textile printing for best expression of the fine lines, realistic shapes and peculiar colors of the butterflies in Chosun folk drawings and used diverse materials as materials for printing for diversity of the designs. Thereby this thesis is purposed to grope the products with superior competitiveness in the world market by presenting the realistic butterflies in Chosun folk drawings as motives with cultural value native to Korea and applying them to clothing designs.

• 한국식품과학회지
• /
• 제9권4호
• /
• pp.313-316
• /
• 1977

The patterns of saponins of lateral gingengs cultivated different areas and various ginseng products were investigated by quantitative thin-layer chromatography. In the case of ginseng cultivated in the Kum San and Gang Hwa area, some parts of the panaxatriol series of the saponins (peak 6 and 7.8.9) were higher in concentration than in ginseng grown in other areas while the other ingredients were almost the same. In the process of heat treatment the quantity of peak 2 was generally decreased. However, in the case of red and white ginseng, one part of the panaxatriol saponins, peak 6 was increased. This tendency was also found in honeyed ginseng and ginseng tea which were not exposed to sunlight, but the increase was much less. The change in the red and white ginseng which were exposed to sunlight was very substantial. Therefore we can assume that the increase of peak 6 comes about due to the combination of heat treatment and exposure to sunlight, especially due to exposure to sunlight.

• 한국지구과학회지
• /
• 제21권3호
• /
• pp.219-229
• /
• 2000

구성주의 학습은 의미구성의 적극적인 과정이며, 학생 스스로 개인의 흥미와 관심, 능력에 따라 스스로 학습목표를 결정할 수도 있다. 본 연구에서는 구성주의에 입각한 학습 교재와 교수-학습 모델을 개발하고 이를 서울의 한 초등학교 학생들에게 적용하였다. 4학년 2학기 ‘지층과 화석’ 단원을 학생과 교사의 토론을 중심으로 재구성하였으며, 안내-실천-표현-합의-발전의 단계에 따라 1개월 동안 수업을 실시하고 그 결과를 정리해 보았다.

• 대한산업공학회지
• /
• 제2권1호
• /
• pp.37-44
• /
• 1976

It is necessary for developing countries to increase their consolidation of domestic technology in order to improve their economy. In order to raise their techniques, they have to try to induce the advanced know-how from other countries in spite of heavy cost. The object of this study is to establish the model on which we base our choice of the proper techniques or plants and give priority to them by using quantified selection criteria. The method in this study has two stages, and the writer has selected 12 factors affecting the decision making for the importation of technology from the industrially advanced countries. First, the lists of valuable know-how for the better development of national industry should be determined, and for the formulation and arrangement of the lists, a council of specialists which uses questionnaires in terms of the Semantic Differential Method, should be organized. Second, for the assignments of priority to the prospective items for importation, the writer has imployed both the Leontief Model and the Disman Model as objective methods and Mottley-Newton method, one of the R&D Project Selection Methods, as a general model. The writer has applied the methods described above to the fields of petrochemical industry in Korea.

• 한국정보통신학회논문지
• /
• 제8권8호
• /
• pp.1818-1827
• /
• 2004

본 논문에서는 압전평면판에 홈을 파 형성한 (equation omitted)형 선형배열음원과 최적화 알고리즘을 조합한 적응 지향성합성 시스템의 2차원 지향성합성 문제를 수치적으로 검토하였다. 임의로 설정한 목적 지향성을 실현하기 위하여 먼저, 선형배열 진동자의 최적 진동 변위량은 최적화 알고리즘인 DFP (Davidon-Fletcher-Powell) 법을 이용하여 계산하고, 다음에, 계산된 진동 변위량을 구동하기 위한 최적 전위의 계산은 유한요소법과 DFP법을 이용하여 행했다. 또한, 유한요소법을 이용하여 (equation omitted)형 압전 트랜스듀서의 2차원 진동해석을 행했다.

• 농촌계획
• /
• 제12권2호
• /
• pp.65-73
• /
• 2006

This study attempts to develop an interim evaluation system for the Integrated Rural Village Cluster Development Project(IRVCDP) which can secure its identity and sustainability, and induce its early settlement, and to derive plans for its steady progress. The evaluation system consists of three levels. The highest-order level is evaluation purposes, which describe IRVCDP's goals. They are expansion of income bases(EIB), improvement of living environments(ILE), strengthening of regional capabilities(SRC) and constructing effective project operation system(CEPOS). The second level is evaluation items, which each characterize the relevant evaluation purposes. The last level is evaluation indices, which observe achievement f3r evaluation purposes. 22 evaluation indices are selected to satisfy the purposes of this study through a brainstorming process and a survey on opinions of experts for those evaluation purposes and evaluation items which are derived from analyzing the IRVCDP's properties. The relative importance of the evaluation indices selected are determined through the AHP analysis. The most intriguing result from the AHP analysis is that strengthening of regional capabilities is found to be the most important one among evaluation purposes. It can be said that this result is parallel to a ultimate goal of the IRVCDP.

• 한국농촌건축학회논문집
• /
• 제12권3호
• /
• pp.19-26
• /
• 2010

This study is aimed to look into the craftsmanship non-educated constructors in Korean-style secondary stations(Gong-So) of Naepo region which is located in the northwestern Chungcheongnam-do. Although the area was persecuted as one of three cradles of Korean Catholicism, Catholic activities flourished there. The 13 cases of Korean-style secondary stations were selected through a survey report list about modern cultural assets of Chungcheongnam-do published in december 2004. This study examined the methods of plan layout and framework of Gong-So to look into craftsmanship of non-educated constructors, the results are as follows; The methods of plan layout are divided into three types according to existence and arrangement of inner column(Nae-Ju); 'Layout with Nae-Ju' is considered as the primary form of Korean-style Gong-So with using regular Nae-Ju. 'Layout with the different position of the center between Nae-Ju and exterior column(Oe-Ju)' and 'layout without Nae-Ju' is appeared in Korean-style Gong-So which is important to assembly and alter function. The methods of framework are appeared variously according to different plan layout for securing worship space. The unusal beam(Chung-Ryang) is used to solve different spans of altar and narthex. When space was formed widely without Nae-Ju, diagonal members and M letter type rafters are used, they are influenced by the technique of western wooden roof truss.

• 대한바이러스학회지
• /
• 제28권1호
• /
• pp.11-20
• /
• 1998

Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38) in Rattus sp. and 0.6% (4/677) in bats. Reciprocal mean IFA titers ranged from 27.5 to 1,024 at the specimen collection. According to the growth rate and reaching peak titier of infectivity, the isolates were grouped as rapid, intermediate, and slow growing groups. All isolates were confirmed as Hantaan type by the nested RT-PCR on the G1 region of the M segment. Comparison of nucleotide sequence (Nt: 2101 - Nt: 2280) of the G2 region revealed that the sequence homology bewteen Hantaan 76/118 virus and the isolates was more than 90%. Several nucleotide positions of the isolates showed high variation. The variation rate of patientisolates was about one-half when compared with that of rodentisolates. On the basis of phylogenetic analysis Hantaan viruses isolated were divided into two genogroups. These results indicate that Hantaan virus is highly dominant serotype in Korea and the virologic property and genogroup are not correlated.