• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.5
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• pp.1260-1270
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• 2007

The discovery of drugs on the treatment of mast cell-mediated allergic disease is a very important subject in human heath. The Socheongyoug-tang(SCYT) has been used for centruries as a traditional medicine in Korea and is known to have an anti-inflammatory effect. However, its specific mechanism of action is still unknown. In this report, we investigated the effect of hot water extract from SCYT on RBL-2H3 mast cell-mediated allergic reaction and studied its possible mechanism of action. SCYT inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. SCYT decreased the passive cutaneous anaphylaxis reaction activated by Anti-lgE antibody-HSA. SCYT dose-dependently reduced histamine release from mice peritoneal mast cells activated by Anti-lgE antibody-HSA. SCYT increased cAMP and decreased compound 48/80-induced intracellular $Ca^{2+}$ levels. Our findings provide evidence that SCYT inhibits mast-derived allergic reactions, and also demonstrate the involvement of cAMP and intracellular $Ca^{2+}$ in these effects.

• The Journal of Internal Korean Medicine
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• v.25 no.2
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• pp.174-182
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• 2004

Object : This study investigates Jaeumganghwa-tang(JGT) has been used for the purpose of prevention and treatment of allergic inflammatory diseases. This study was to investigate the biological effects of JGT. Methods : Cytotoxcicity and inflammatory cytokines secretion with human mast cells(HMC-1) were examined. HMC-1 cells were stimulated with phorbol l2-myristate 13-acetate (PMA) and calcium ionophore A23l87. JGT by itself had no effect on cytotoxicity of HMC-1. The effects of JGT on the secretion of tumor necrosis factor-alpha(TNF-${\alpha}$) and interleukin(IL)-6 from HMC-1 were evaluated with enzyme-linked immunosorbent assay(ELISA). Result : It was found that JGT inhibited PMA plus A23187-induced TNF-${\alpha}$ and IL-6 secretion. JGT also inhibited the $NF-{\kappa}$B(p50) expression. Conclusion : These results suggest that JGT inhibits the secretion of inflammatory cytokines in HMC-1 cells through blockade of $NF-{\kappa}B$ activation. Taken together, these effects support a role for JGT as a therapeutic agent in treatment of allergic inflammatory diseases such as asthma.

• The Journal of Pediatrics of Korean Medicine
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• v.23 no.1
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• pp.1-22
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• 2009

Objectives The purpose of this study is to investigate the effect of Li Zhong Tang(LZH-T) on atopic dermatitis by using NC/Nga atopic dermatitis mouse. Methods First, in vitro, we isolated B cells from NC/Nga mouse which induced atopic dermatitis-like skin for 18 weeks. We analyzed FACS by intracellular staining of $IFN-{\gamma}$, GATA-3+ and also analyzed cytokines by using real-time PCR. Secondly, in vivo, after administration of LZH-T to the 12 weeks old mouse with atopic dermatitis. We analyzed serum IgE, $IFN-{\gamma}$ level and observed the changes of activated cell. Results In vitro, LZH-T decreased the levels of CD4+/$IFN-{\gamma}$ and increased the levels of CD4+/GATA3+. In vivo, serum IgE levels were decreased and $IFN-{\gamma}$ levels were increased in LZH-T group compared to the control group. In PBMCs, the percentage of activated cell - granulocytes, CD3+, CD3+CD4+, B220+CD23+, and CCR3+ were decreased and CD19+, CD3+CD8+ were increased in LZH-T group compared to the control group. Conclusions This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

• CELLMED
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• v.4 no.2
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• pp.14.1-14.6
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• 2014

KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.

• Herbal Formula Science
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• v.23 no.2
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• pp.171-187
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• 2015

Objective This study aimed to evaluate the effects of Sagan-tang (SGT) on COPD mouse model. Methods The study was carried out by two ways (in vitro, in vivo). In vitro RAW264.7 cells (mouse macrophage) were used and analysed by flow cytometry, ELISA, Western blot. In vivo LPS and CSS challenged mice were used and its BALF had been analysed by cytospin image, FACS, ELISA, lung tissue by real-time PCR. Results In vitro, SGT maintained 80-100% rate of viablilty on 10 ~ 500 ㎍/㎖ concentration. In ELISA analysis with RAW264.7 cells, SGT significantly decreased NO over 30 ㎍/㎖. In flow cytometry, SGT 100 ㎍/㎖ dosage group displayed a tendency for decrease ROS. In Western blot analysis, SGT 100 ㎍/㎖ dosage group decreased NF-κB. In ELISA analysis, SGT significantly decreased TNF-α, IL-6 over 200 ㎍/㎖. In vivo SGT 200 ㎎/㎏ dosage group, application of SGT significantly decreased increase of neutrophils, TNF-α, IL-6 in BALF, muc5AC, TGF-β, TNF-α, expression of mRNA in lung tissue and histological lung injury. Conclusion This Study suggests usability of SGT for COPD patients by controlling lung tissue injury.

• The Journal of Internal Korean Medicine
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• v.40 no.5
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• pp.983-989
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• 2019

Objective: Many types of delayed adverse reactions, such as long-term numbness, occur after injecting contrast medium, but few clinical studies have reported effective treatments for these reactions. We report that herbal medicine can have a positive clinical effect in patients who have experienced long-term numbness after contrast medium injection. Method: A patient who had felt numbness in her head and whole limbs presented at Semyung University Korean Medicine hospital. Her numbness had first started 2 days after injection of contrast medium, and she had felt it constantly for almost 2 years. After admission at the hospital, she took "modified Samul-tang" herbal medicine (120 cc three times per day) from 29 January 2019 to 2 February 2019. We measured her numbness using a numeric rating scale (NRS) on 29 January and 2 February and we compared both scores to determine the degree of favorable and clinical effects of herbal medicine. Result: The NRS score for numbness was 5 for her head and limbs on the first day (29 January), but after 4 days (2 February), the NRS score decreased to 0 for her head and both arms and hands, and it decreased from 5 to 2 for both her legs and feet. Conclusion: Korean herbal medicine can have positive clinical effects on treating adverse reactions due to injection of contrast medium.

• The Journal of Pediatrics of Korean Medicine
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• v.23 no.2
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• pp.87-101
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• 2009

Objectives : The purpose of this study is to investigate the effect of JUJSTK on atopic dermatitis in an in-vitro experiment using an NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the humans in terms of health condition. Methods : We evaluated IL-1$\beta$, IL-6, IL-10, TNF-$\alpha$ mRNA, TGF-$\beta$ mRNA, CD4+/IFN-$\gamma$+ and IL-17+CD4+Th17 cells of NC/Nga atopic dermatitis mouse by real-time PCR and intracellular staining in vitro. Results : JUJSTK medicines supressed the activities of IL-1$\beta$, IL-6, TNF-$\alpha$, TGF-$\beta$ mRNA and IL-17+CD4+Th17 cells and it incresed the activities of IL-10 mRNA in B cells. The level of CD4+/IFN-$\gamma$+ in T cells were increased by JUSSTK. Conclusions : JUJSTK on atopic dermatitis might be incredibly effective to the atopic dermatitis treatment.

• 대한상한금궤의학회지
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• v.5 no.1
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• pp.61-73
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• 2013

Objective : The purpose of this report is to submit a case study on a patient of developmental disabilities with ADHD by taking "Sihogayonggolmoryeo-tang". Methods : We gave herbal medicine to a patient and observe the progress as the way of Cornners Abbreviated Rating Scale(CARS), ADHD Rating Scale (K-ARS), The ADD-H Comprehensive Teacher's Rating Scale(ACTeRS), Child Attention Problem(CAP), IOWA Conners Rating Scale, Conners Teacher Rating Scale(CTRS), Conners Parents Rating Scale (CPRS), Home Situation Questionnaire(HSQ), School Situation Questionnaire(SSQ). Results : The total score of CARS, ACTeRS and other scales were decreased by taking herbal medicine, and especially hyperactive scores were significantly decreased. Conclusions : Patient's symptom was significantly improved by using "diagnostic system based on Shanghanlun six meridian patterns and provisions" and analyzing patient's psychological and physical conditions.

• The Journal of Pediatrics of Korean Medicine
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• v.14 no.2
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• pp.47-60
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• 2000

The purpose of this research was to investigate the effects of Kami Kwiryong Tang (KKT) on the immune cells in BALB/c mice. KKT (500mg/kg) was administerd p.o. once a day for 7 days. KKT decreased the proliferation of thymocytes, but did not affect the proliferation of splenocytes. KKT enhanced the subpopulation of Th (CD4+CD8- single positive cells) cells in splenic T-lymphocytes, but decreased the subpopulation of Th cells in thymocytes. KKT enhanced the production of ${\gamma}$-interferon and interleukin-2, but did not affect the production of interleukin-4 in mice serum. KKT did not affect the production of nitric oxide, but enhanced the phagocytic activity in peritoneal macrophages. These results suggest that KKT is a potent prescription on immune response via the production of cytokines from splenic Th1 cells and the increase of phagocytic activity in vivo.

• Advances in Traditional Medicine
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• v.2 no.1
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• pp.17-27
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• 2002

Obesity is a serious medical disorder because it can cause a myriad of health problems, such as heart disease, hypertension, and adult-onset diabetes. While conventional medical treatment for obesity has limitations, there is enormous public enthusiasm for complementary and alternative treatments of obesity. This article reviews currently commonly used complementary and alternative therapies for obesity, such as medicinal herbs, nutritional supplements and acupuncture. Beneficial effects and possible adverse effects associated with these treatment are discussed.