• 한국토양비료학회지
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• 제35권6호
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• pp.335-343
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• 2002

우리나라 유통상토에 대한 물리적 표준분석법을 설정하기 위한 연구를 실시하였다. 국내 시중에 유통되고 있는 53개의 원예용 상토와 9개의 수도용 상토를 대상으로 설정된 분석법을적용하여 실험하였다. 수분함량은 $105^{\circ}C$ 건조기에서 16시간 건조하여 수도용상토는 (건조전 무게-건조 후 무게) / (건조 후 무게) ${\times}100$으로, 원예용상토는 (건조 전 무게 - 건조 후 무게 ) / (건조전 무게) ${\times}100$ 으로 하였다. 보수력은 주로 저 압용으로서 Eijkelkamp사의 Sandbox법을 사용하여 0.5, 1, 3, 5, 7, 10 kPa의 수분장력 상태의 수분함량을 조사하였다. 포화수리전도도는 직경 5cm${\times}$높이 20cm인 아크릴 원통을 이용하여 정수위별에 의한 포화수리전도도를 측정하였다. 신분석법에 의한 유통상토의 수분함량은 원예용이 평균 46.34%, 수도용이 평균 16.89%이었고 원에용 상토의 EAW는 28.4%, WBC 7.01%, OMP는 5.60 kPa로 나타났으며, 육묘를 위한 적정 수분함량은 44.41% 이었고 포화수리전도도는 $1.71cm\;min^{-1}$이었다.

• Journal of Microbiology and Biotechnology
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• 제20권8호
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• pp.1243-1250
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• 2010

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Ni-charged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PA-CPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5${\alpha}$ harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5${\alpha}$ and control.

• 조명전기설비학회논문지
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• 제15권1호
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• pp.84-89
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• 2001

본 논문은 다중 해상도 웨이브렛 변환을 이용하여 코로나 방전과 코로나방전 후 연면방전을 거치는 다중결함에 대한 방전전류펄스의 변화량을 시간 대역과 주파수 대역에서의 변화량을 분석하였다. 마더웨이브렛의 선정은 방전전류펄스의 형태를 고려하여 Daubechies 마더웨이브렛을 선정하였으며, 방전전류펄스는 이산 웨이브렛 변환을 이용하여 근사신호와 상세신호로 4단계까지 다중분해되었다. 이중 상세신호만을 이용하여 12개의 세그먼트로 나눈후 설정한 변수에 따라 그 패턴을 파악하였다. 그 결과 코로나 방전에서는 설정된 변수의 세그먼트 7, 8, 9, 10의 값이 방전의 진전에 따라 증가하였으므로 위상분포를 $210~330[^{\circ}]$분포를 특징지울 수 있고, 에폭시 절연체를 삽입한 다중결함에서는 연면방전의 특징에 기인하여 설정된 변수의 값이 대칭적인 패턴을 나타내었다.

• Journal of Microbiology
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• 제43권spc1호
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• pp.110-117
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• 2005

Salmonella enterica serovar Typhimurium causes human gastroenteritis and a systemic typhoid-like infection in mice. A critical virulence determinant of Salmonella is the ability to invade mammalian cells. The expression of genes required for invasion is tightly regulated by environmental conditions and a variety of regulatory genes. The hilA regulator encodes an OmpR/ToxR family transcriptional regulator that activates the expression of invasion genes in response to both environmental and genetic regulatory factors. Work from several laboratories has highlighted that regulation of hilA expression is a key point for controlling expression of the invasive phenotype. A number of positive regulators of hilA expression have been identified including csrAB, sirA/barA, pstS, hilC/sirC/sprA, fis, and hilD. HilD, an AraC/XylS type transcriptional regulator, is of particular importance as a mutation in hilD results in a 14-fold decrease in chromosomal hilA::Tn5lacZY-080 expression and a 53-fold decrease in invasion of HEp-2 cells. It is believed that HilD directly regulates hilA expression as it has been shown to bind to hilA promoter sequences. In addition, our research group, and others, have identified genes (hilE, hha, pag, and lon) that negatively affect hilA transcription. HilE appears to be an important Salmonella-specific regulator that plays a critical role in inactivating hilA expression. Recent work in our lab has been directed at understanding how environmental signals that affect hilA expression may be processed through a hilE pathway to modulate expression of hilA and the invasive phenotype. The current understanding of this complex regulatory system is reviewed.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.558-568
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• 2023

Vibrio vulnificus is an aquatic bacterium causing septicemia and wound infection in humans. To understand this pathogen at the genomic level, it was performed whole genome sequencing of a cefoxitin-resistant strain, V. vulnificus 1908-10 possessing virulence-related genes (vvhA, viuB, and vcgC) isolated from Gadeok island coastal seawater in South Korea. The genome of V. vulnificus 1908-10 consisted of two circular contigs and no plasmid. The total genome size was estimated to be 5,018,425 bp with a guanine-cytosine (GC) content of 46.9%. We found 119 tRNA and 34 rRNA genes respectively in the genome, along with 4,352 predicted protein sequences. Virulence factor (VF) analysis further revealed that V. vulnificus 1908-10 possess various virulence genes in classes of adherence, antiphagocytosis, chemotaxis and motility, iron uptake, quorum sensing, secretion system, and toxin. In the comparison of the presence/absence of virulence genes, V. vulnificus 1908-10 had fur, hlyU, luxS, ompU, pilA, pilF, rtxA, rtxC, and vvhA. Of the 30 V. vulnificus comparative strains, 80% of the C-genotype strains have all of these genes, whereas 40% of the E-genotype strains have all of them. In particular, pilA were identified in 80% of the C-type strains and 40% of the E-type strains, showing more difference than other genes. Therefore, V. vulnificus 1908-10 had similar VF characteristics to those of type C strains. Multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin of V. vulnificus 1908-10 contained 8 A-type repeats (GXXGXXXXXG), 25 B.1-type repeats (TXVGXGXX), 18 B2-type repeats (GGXGXDXXX), and 7 C-type repeats (GGXGXDXXX). The National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) showed that the RtxA protein of V. vulnificus 1908-10 had the effector domain in the order of cross-liking domain (ACD)-C58_PaToxP-like domain- α/β hydrolase-C58_PaToxP-like domain.

• 콘크리트학회지
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• 제7권3호
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• pp.156-163
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• 1995

앞 선 연구에서는 스티렌과 무수말레인산으로부터 스티렌-무수말레인산 공중합체(SMA)를 합성하고 이들을 황산화하여 수용성의 SMA를 제조하였다. 본 연구에서는 이들 카르본산계 공중합체를 첨가한 시멘트 모르타르의 플로우 및 경화시멘트 모르타르의 강도를 조사하여 고성능감수제로서의 성능을 평가하였다. 플로우 실험 결과 황산화 SMA(SSMA)를 첨가한 시멘트 모르타르의 플로우는 아미노페놀이 치환된 황산화 SMA(SmSMA)를 첨가한 경우보다 더 큰 값을 나타내었다. 또한 공중합체를 첨가한 시멘트 모르타르의 플로우 유지율은 기존의 나프탈렌계(NSC)를 첨가한 경우보다 우수하게 나타났다. 시멘트 모르타르에 SMA와 SmSMA를 시멘트 중량에 대해 0.5% 첨가하여 제조한 경화 시멘트 모르타르의 28일 압축강도를 조사하였다. 그 결과 SSMA 및 SmSMA를 첨가한 경우 plain 보다 각각 31%와 13%의 강도 증가를 나타내었다. 이상의 결과로부터, 본 연구에서 사용한 SSMA 및 SmSMA의 카르본산계 공중합체는콘크리트용 고성능감수제로서 크게 기대되어진다.

• 한국통신학회논문지
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• 제27권1C호
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• pp.103-111
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• 2002

IMT-2000에서 RNC의 Main Control Processor는 호 처리를 담당하는 부분으로, 고신뢰도와 실시간성이 요구되므로 결함 허용 시스템의 연구가 중요하다. 이를 위하여 본 연구에서는 태스크 기반 이중화 방안을 제안한다. 이 방안은 Active side의 태스크들이 메시지 단위로 동작하고, 동작 후 변경된 메모리 영역의 데이터를 Standby side에 전달하는 방식을 기본으로 하며, 절체 시 recovery를 위해 메시지를 logging하는 방식이다. 제안한 방식은 dual down 및 동기화 과정의 복잡성을 제거 할 뿐만 아니라, 태스크가 동기를 제어하므로 좀 더 정확한 동기화가 가능하다. 또한 효과적으로 태스크 기반 이중화를 수행하기 위한 결함 탐지 및 처리 방안을 제시한다. 이 방안은 결함 탐지 확률을 높이고 결함에 의하여 발생한 오류 데이터가 Standby side로 전송되는 것을 원천적으로 차단하는 것에 중점을 둔다.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.470-478
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• 2012

Recent genome comparisons of E. coli B and K-12 strains have indicated that the makeup of the cell envelopes in these two strains is quite different. Therefore, we analyzed and compared the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots, including 62 nonredundant proteins, were unambiguously identified by two-dimensional gel electrophoresis and mass spectrometry. Of these, 43 proteins were conserved between the two strains, whereas 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains were as follows: (i) B produced more OmpF porin with a larger pore size than K-12, indicating an increase in the membrane permeability; (ii) B produced higher amounts of lipoproteins, which facilitates the assembly of outer membrane ${\beta}$-barrel proteins; and (iii) motility- (FliC) and chemotaxis-related proteins (CheA and CheW) were detected only in K-12, which showed that E. coli B is restricted with regard to migration under unfavorable conditions. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies.

• 천문학회지
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• 제50권5호
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• pp.139-149
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• 2017

The Korea Astronomy and Space Science Institute plans to develop a coronagraph in collaboration with National Aeronautics and Space Administration (NASA) and to install it on the International Space Station (ISS). The coronagraph is an externally occulted one-stage coronagraph with a field of view from 3 to 15 solar radii. The observation wavelength is approximately 400 nm, where strong Fraunhofer absorption lines from the photosphere experience thermal broadening and Doppler shift through scattering by coronal electrons. Photometric filter observations around this band enable the estimation of 2D electron temperature and electron velocity distribution in the corona. Together with a high time cadence (<12 min) of corona images used to determine the geometric and kinematic parameters of coronal mass ejections, the coronagraph will yield the spatial distribution of electron density by measuring the polarized brightness. For the purpose of technical demonstration, we intend to observe the total solar eclipse in August 2017 with the filter system and to perform a stratospheric balloon experiment in 2019 with the engineering model of the coronagraph. The coronagraph is planned to be installed on the ISS in 2021 for addressing a number of questions (e.g., coronal heating and solar wind acceleration) that are both fundamental and practically important in the physics of the solar corona and of the heliosphere.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2079-2094
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• 2018

Sunflower trypsin inhibitor (SFTI) is a 14-amino-acid bicyclic peptide that contains a single internal disulfide bond. We initially constructed chimeras of SFTI with N-terminal secretion signals from the Escherichia coli OmpA and Pseudomonas aeruginosa ToxA, but only detected small amounts of protease inhibition resulting from these constructs. A substantially higher degree of protease inhibition was detected from a C-terminal SFTI fusion with E. coli YebF, which radiated more than a centimeter from an individual colony of E. coli using a culture-based inhibitor assay. Inhibitory activity was further improved in YebF-SFTI fusions by the addition of a trypsin cleavage signal immediately upstream of SFTI, and resulted in production of a 14-amino-acid, disulfide-bonded SFTI free in the culture supernatant. To assess the potential of the secreted SFTI to protect the ability of a cytotoxic protein to kill tumor cells, we utilized a tumor-selective form of the Pseudomonas ToxA (OTG-PE38K) alone and expressed as a polycistronic construct with YebF-SFTI in the tumor-targeted Salmonella VNP20009. When we assessed the ability of toxin-containing culture supernatants to kill MDA-MB-468 breast cancer cells, the untreated OTG-PE38K was able to eliminate all detectable tumor cells, while pretreatment with trypsin resulted in the complete loss of anticancer cytotoxicity. However, when OTG-PE38K was co-expressed with YebF-SFTI, cytotoxicity was completely retained in the presence of trypsin. These data demonstrate SFTI chimeras are secreted in a functional form and that co-expression of protease inhibitors with therapeutic proteins by tumor-targeted bacteria has the potential to enhance the activity of therapeutic proteins by suppressing their degradation within a proteolytic environment.