• 한국조경학회지
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• 제41권5호
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• pp.19-27
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• 2013

본 연구는100만평공원운동의 시민참여, 홍보, 발전에 주도적인 역할을 한 100만평문화공원조성 범시민협의회의 리더십에 근거하여 100만평공원은 일반적인 시민참여형공원과 차별화되는 시민주도형공원이라 재조명한다. 100만평문화공원 조성 범시민협의회는 2001년 운동 초기부터 현재까지 일반 시민을 대상으로 각종 문서홍보물 출판하여 100만평공원운동, 시민참여, 도심공원의 가치와 필요성을 간접 교육하였다. 특히, 문서홍보물의 내용 중 100만평공원 조성의 정당성을 밝히기 위해 100만평문화공원조성 범시민협의회가 제시하는 공원조성 공략문은 부산내 공원자원의 현주소와 문제점을 지적하고, 도심공원의 필요성을 강조한 시민의 목소리가 담긴 사회적 문헌으로 고려하였다. 수집된 공략문은 담론분석(discourse analysis) 방법을 이용하여 담론의 변천과정을 100만평공원운동 태동기와 맹아기(1999~2001.2), 성장기(2001.2~2008), 100만평공원 대상지의 그린벨트 해제 이후(2008~현재) 세 개의 기점을 기준으로 분석하였다. 담론분석 결과, 각각의 담론에서 100만평공원운동은 1) 부산의 공공녹지 환경을 개선하기 위한 시민참여형 공원에서 2) 자연체험과 환경교육을 통한 시민참여확장형 공원 그리고 3) 전국적 민관 네트워크를 기반으로 한 국가공원운동의 순서로 발전하였다. 각각의 담론은 100만평 문화공원조성 범시민협의회가 부산의 공원자원의 문제점과 환경적 이슈를 지적하고 그 해결책으로 100만평공원을 제시 및 합리화하는 구조로 구성된다. 본 논문은 부산 시민들로 하여금 지역의 환경문제에 관한 비판적 이해를 돕고 도심공원의 가치를 간접 교육한 100만평공원 담론의 역할을 강조하고, 담론내용을 분석하여 구체적인 사회학습적 가치를 설명한다.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.854-861
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• 2014

The diagnosis of Brucella abortus is mainly based on serological methods using antibody against LPS, which has diagnostic problems. Therefore, to solve this problem, we evaluated two proteins of B. abortus, Cu/Zn superoxide dismutase (SodC) and outer membrane proteins 2b porin (Omp2b). The genes were cloned and expressed in a pMAL system, and the recombinant proteins, rOmp2b and rSodC, were purified as fusion forms with maltose-binding protein. The identity of the proteins was confirmed by SDS-PAGE and Western blot analysis with sera of mice infected with B. abortus. Production of cytokines and nitric oxide (NO) was investigated in RAW 264.7 cells and mouse splenocytes after stimulation with the proteins. Moreover, cellular and humoral immune responses were investigated in BALB/c mice after immunization with the proteins. TNF-${\alpha}$, IL-6, and NO were significantly inducible in RAW 264.7 cells. Splenocytes of naive mice produced IFN-${\gamma}$ and IL-4 significantly by stimulation. Moreover, number of IgG, IFN-${\gamma}$, and IL-4 producing cells were increased in immunized mice with the two proteins. Production of IgG and IgM with rOmp2b was higher than those with rSodC in immunized mice. These results suggest that the two recombinant proteins of B. abortus may be potential LPS-free proteins for diagnosis.

• 한국해양바이오학회지
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• 제2권2호
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• pp.123-125
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• 2007

Vibrio anguillarum is a gram-negative bacterium that causes vibriosis in approximately 80 different fish species. V. anguillarum produces several exotoxins are correlated with the pathogenesis of vibriosis. This study is focused on the composition of the outer membrane vesicle. Most of gram-negative bacteria produce outer membrane vesicle (OMV) during cell growth. OMV was formed from the outer membrane surface of cell and than released to extracellular environment. OMV consists of outer membrane lipids, outer membrane protein (OMP), LPS, and soluble periplasmic components. Also, they contain toxins, adhesions, and immunomodulatory. Many gram-negative bacteria were studied out forming OMV. In Vibrio sp., formation of OMV by electron microscopy has been reported from V. cholerae and V. parahaemolyticus. In present study, we isolated OMV from V. anguillarum and OMV protein was separated by SDS-PAGE. Magor band was sliced and analyzed by MALDI-TOF. The major protein band of 38kDa was identified as OmpU by MALDI-TOF MS analysis.

• 한국동물위생학회지
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• 제33권2호
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• pp.135-141
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• 2010

Bovine brucellosis, an important zoonosis, is diagnosed with serological tests such as the RBT, TAT using inactivated whole bacterial cells or bacterial lipopolysaccharide (LPS) antigen in Korea. However, a strong cross-reaction between Brucella spp. and Yersinia enterocolitica O9 in these tests has seriously complicated the diagnosis of animal brucellosis because Brucella spp. shares common antigenic determinants with Y. enterocolitica O9 in the smooth LPS region. In this study, Brucella-field strains were isolated from Brucellapositive Hanwoo in Kimhae, Korea and outer membrane protein (omp) which has low cross-reaction with Y. enterocolitica O9 and high immunogenicity was extracted from the field strains Then we compared ELISA using the extract with RBT-TAT. Fifteen field strains were isolated from 47 supra-mammary-lymph nodes, which were collected from 18 farms. Isolation rate was 32%. Brucella-specific antigen was identified by performing SDS-PAGE or Western blotting on extracted omp with at 0.5% n-lauroylsarcosine One hundred and ninety-two serum-samples were used in the experiment: 142 negative and 50 positive samples verified by RBT-TAT. According to ELISA results, 127 samples were negative and 15 appeared positive among 142 negatives by RBT-TAT, while 42 samples were positive and 8 were negative among 50 positives by RBT-TAT. Therefore, it showed 89.4% of specificity and 84% of sensi-tivity. Through the current experiments, we could set up an ELISA based on the omp which has low cross-reaction and high immunogenicity and concluded that the omp could be a good material for accurate diagnosis of bovine brucellosis.

• 대한예방한의학회지
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• 제4권1호
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• pp.1-16
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• 2000

From 1998, Oriental Medical Physicians(OMP) is distributed in Public Health Sector. but long term plan for Oriental Medicine in Public Health is not existed. So, this study is designed for the activation of OMP Subjects in this study were comprised 3 groups of oriental medicine related persons, the group of which are Students of 11 Oriental Medical Schools, Oriental Medical Physicians in Public Health Sector, Specialist Croup of Oriental medicine Policy(total 1,458 persons). Data were collected from July 1st to November 30, 1999. Direct interview with key persons, systematic interview by using of interview protocol, e-mail and facsimile have been conducted. The results of survey were coded by Excel 5.0, and analysed with SAS 6.12 statistical package. Inter-group difference determined by T-test, and descriptive statistics have been examined. Major findings can be epitomized as follows. 1) OMP disposition to multifarious organizations and institutes such as Public Health Centers, Public Health Sub-centers, Public Hospitals, Private Hospitals in Remote-Vulnerable Area, Community Social Welfare Centers, Institutes for Heath Policy Research, etc, will promise an effective use of Oriental Medical Physician. 2) Average number of patients treated by OMP was 22.8, average budget for oriental medical department, in which OMP were affiliated, was 39.6 million Won per year. Direct cost per every patient visit was 7,210.9 Won, which is considered expensive for public health service. Therefore, development and transformation for Oriental Medical Service in Public Health Sector is desirable in economic and political aspects. 3) It is recommended that ${\ulcorner}Advisory\;Committee{\lrcorner},{\ulcorner}Planning\;Commission\;for\; Public\;Health\;in\;Oriental\;Medicine{\lrcorner}$ should be established for the activation and for the support of Oriental Medical Physician in Public Health Sector, 4) Most effective programmes for oriental public health doctor are health service programmes for the elderly, Home health visit, chronic degenerative disease control services(p<0.001). 5) Standard guideline for facilities and equipments of Oriental Medicine Department in Public Health Center is needed for optimal supply of resources and activation of public health activity.

• ETRI Journal
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• 제42권6호
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• pp.815-826
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• 2020

We propose a two-dimensional (2D) scattering-center-extraction (SCE) method using sparse recovery based on the compressive-sensing theory, even with data missing from the received radar cross-section (RCS) dataset. First, using the proposed method, we generate a 2D grid via adaptive discretization that has a considerably smaller size than a fully sampled fine grid. Subsequently, the coarse estimation of 2D scattering centers is performed using both the method of iteratively reweighted least square and a general peak-finding algorithm. Finally, the fine estimation of 2D scattering centers is performed using the orthogonal matching pursuit (OMP) procedure from an adaptively sampled Fourier dictionary. The measured RCS data, as well as simulation data using the point-scatterer model, are used to evaluate the 2D SCE accuracy of the proposed method. The results indicate that the proposed method can achieve higher SCE accuracy for an incomplete RCS dataset with missing data than that achieved by the conventional OMP, basis pursuit, smoothed L0, and existing discrete spectral estimation techniques.

• 한국축산식품학회지
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• 제23권2호
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• pp.161-167
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• 2003

사람들에게 계란과 관련하여 일어나는 S. enteritidis(SE)와 S. typhimurium(ST)의 전파를 줄이기 위해서 감염된 상업적인 산란계군을 찾는 것은 매우 중요한 일이다. 그러므로 본 연구는 국내 산란계군에서 생산되는 계란의 주된 식중독균인 SE와 ST의 청정화 관리를 위한 기초 연구로서 SE와 ST에 대한 산란계군의 감염 여부를 파악하기 위해 시중에 유통하는 계란의 난황에 존재하는 SE와 ST에 대한 특이 항체를 ELISA방법으로 조사하였다. 시중에 유통되는 3개 제품의 계란의 난황을 1:100부터 1:1,600까지 두배 희석법으로 희석한 후 SE균체와 ST균체 및 ST의 OMP항원에 대해 특이 항체 역가를 ELISA로 측정한 결과 대조군에 비해 희석곡선의 형태가 차이가 나는 것을 알 수 있었다. 시중에 유통중인 2개 제품의 계란을 1:200과 1:1,000으로 희석한 후 ST항원, OMP항원과 SE항원에 대한 각각 ELISA를 실시한 결과 각각의 항원에 대해 난황특이 항체에 대한 역가는 같은 제품일지라도 차이가 있었다. 21개 제품의 계란을 각 회사마다 5개씩 무작위로 추출하여 계란난황을 1:1,000으로 희석한 후 3가지 항원 ST균체, SE균체 및 ST의 OMP 항원에 대해 ELISA을 실시한 결과 SE와 ST균체 항체보다 OMP항원에 대해 전반적으로 높은 항체 역가를 나타냈으며, ST는 30%, SE는 28.5%, OMP는 28.5% 수준에서 대조구와 비슷한 낮은 역가의 항체 값을 나타내었다. 본 논문의 결과는 난황항체 역가를 측정함으로서 산란계군에서 ST와 SE에 오염을 파악하거나 ST와 SE의 오염을 줄이는데 효과적으로 이용될 수 있는 민감한 방법이라고 생각된다.X>$10^{8}$ g ~ 3.05$\times$$10^{8}$ g 분포를 나타냈으며, 생녹각김치, 유산처리 녹각김치, 대조김치, 열수추출 녹각김치의 순으로 많았다. 녹각 김치를 사람들에게 맛보게 하여 짠맛, 신맛, 향기, 색, 조직감을 평가시킨 결과 열수추출 녹각김치와 생녹각김치가 가장 높은 값을 나타냈고 이어서 대조김치와 유산처리 녹각김치의 순을 나타냈다.TEX>0.13)은 무첨가 초콜릿(3.25$\pm$0.15)에 비해 모두 유의적(P<0.001)으로 낮은 점수를 나타냈으나, 2% 첨가수준에서는 맛, 향, 조직감 및 기호도에서 유의적인 차이를 보이지 않았다.의 소규모 와, 강한조류 및 지형적 특성(불규칙한 해저지형 및 고립도서)에 의해 이루어지는 왕성한 수평 및 연직 혼합 등과 같은 어장학적 호조건에 의해 마라도 주변에 체류하게 되고, 이들 체류어군은 조류방향에 따라 섬의 조상 측에 농밀군을 형성하는 섬의 조상측 어장이다.향을 주지 않는 것으로 나타났으므로 청소년 각자의 식습관 및 식품 섭취에 대한 관심을 고취시킬 필요가 있다고 생각된다.d with an MR peak in the vicinity of the coercive field. The low-field tunnel-type MR characteristics of thin films deposited on different substrates originates from the behavior of grain boundary properties. 않고 단지 안전 보호측면에서의 연구가 이루어졌을 뿐이었다. 따라서 서열환경하에서 머리부분의 쾌적성을 고려한 다양한 작업

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.878-884
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• 2013

Salmonella is a major foodborne pathogen that causes a variety of human diseases. Development of ligands directly and specifically binding to the Salmonella will be crucial for the rapid detection of, and thus for efficient protection from, the virulent bacteria. In this study, we identified a RNA aptamer-based ligand that can specifically recognize Salmonella Typhimurium through SELEX technology. To this end, we isolated and characterized an RNase-resistant RNA aptamer that bound to the OmpC protein of Salmonella Typhimurium with high specificity and affinity ($K_d$ ~ 20 nM). Of note, the selected aptamer was found to specifically bind to Salmonella Typhimurium, but neither to Gram-positive bacteria (Staphylococcus aureus) nor to other Gram-negative bacteria (Escherichia coli O157:H7). This was evinced by aptamer-immobilized ELISA and aptamer-linked precipitation experiments. This Salmonella species-specific aptamer could be useful as a diagnostic ligand against pathogen-caused foodborne sickness.

• 한국동물위생학회지
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• 제23권3호
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• pp.289-299
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• 2000

The study was performed to investigate the distributions of swine pleuropneumonia in Kyongbuk western area by the enzyme-linked immunosorbent assay (ELISA). Sera collected from 400 slaughtered pigs in 3 slaughter houses during the period from May 1999 to october 1999 were tested to detect antibodies against A pleuropneumonie serotype 2 and 5. The optimal dilution of CBE antigen, conjugate and serum for this ELISA were determined 1 : 400, 1 : 20,000, 1 100, respectively. The optimal dilution of OW antigen, conjugate and serum for this ELISA were determined 1 : In, 1 : 20,000, 1 : 200, respectively. Cut-off value in this ELISA was determined by mean absorbance (at 492 nm) of negative control sera added with the triple value of the standard deviation. Cut-off value in ELISA by CBE and OMP antigen were 1.134 and 1.217, respectively. By the ELISA, positive reaction rates to A pleuropneumoniae serotype 2 and 5 for CBE antigen were 38.8% and 18.8%, and for OMP antigen were 42.8% and 23.5% of the 400 samples, respectively.

• Journal of Positioning, Navigation, and Timing
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• 제10권3호
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• pp.169-177
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• 2021

In this paper, we propose a novel global navigation satellite system (GNSS) spoofing detection technique through an array antenna-based direction of arrival (DoA) estimation of satellite and spoofer. Specifically, we consider a sophisticated GNSS spoofing attack scenario where the spoofer can accurately mimic the multiple pseudo-random number (PRN) signals since the spoofer has its own GNSS receiver and knows the location of the target receiver in advance. The target GNSS receiver precisely estimates the DoA of all PRN signals using compressed sensing-based orthogonal matching pursuit (OMP) even with a small number of samples, and it performs spoofing detection from the DoA estimation results of all PRN signals. In addition, considering the initial situation of a sophisticated spoofing attack scenario, we designed the algorithm to have high spoofing detection performance regardless of the relative spoofing signal power. Therefore, we do not consider the assumption in which the power of the spoofing signal is about 3 dB greater than that of the authentic signal. Then, we introduce design parameters to get high true detection probability and low false alarm probability in tandem by considering the condition for the presence of signal sources and the proximity of the DoA between authentic signals. Through computer simulations, we compare the DoA estimation performance between the conventional signal direction estimation method and the OMP algorithm in few samples. Finally, we show in the sophisticated spoofing attack scenario that the proposed spoofing detection technique using OMP-based estimated DoA of all PRN signals outperforms the conventional spoofing detection scheme in terms of true detection and false alarm probability.