• 한국어병학회지
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• 제14권1호
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• pp.3-10
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• 2001

식물성 생약재의 항균력과 활성산소(superoxide anion, $O_2^-$)의 생산에 미치는 in vitro 효과를 조사하여 금후 in vivo 실험에 적용할 때, 어류 체내 자연 저항성 증진을 꾀할 수 있는 식물성 생약재를 발굴하기 위한 기초자료를 얻고자 하였다. 식물성 생약재는 고삼, 귀전우, 구절초, 박하, 방풍, 연교, 약쑥, 지유, 삼백초, 삼지구엽초, 상백피, 소회향, 편축, 팔각 등 14종류로 이들을 열수추출하여 실험에 사용하였다. 항균력 시험에는 어병세균 Listonella anguillarum(Vibrio anguillarum), Vibrio sp., Vibrio alginolyticus, Edwardsiella tarda, Streptococcus sp., Lactcoccus garvieae를 대상으로 disk법을 사용하였으며, 활성산소의 생산능은 넙치 신장 대식세포를 이용한 nitroblue tetrazolium(NBT) 반응으로써 조사하였다. 그 결과,약쑥이 다른 생약재에 비하여 시험에 사용된 어병 세균에 대한 항균활성이 월등히 뛰어났으며, 또한 넙치 대식세포의 활성산소 생산능을 크게 자극하는 효과가 있음을 확인할 수 있었다.

• 수산경영론집
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• 제46권3호
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• pp.119-127
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• 2015

This study is to analyse the causality and volatility spillover between farming fish species in consumption replacement relation using flatfish(oliver flounder) and rockfish's wholesale market price data from September 2006 to July 2015. For the analysis, VAR(5) model and bivariate asymmetric GARCH-BEKK model are employed. The empirical results of this study are summarized as follows: First, the price volatility of flatfish and rockfish is very large without the trend during the sample period. Second, the correlation coefficient between flatfish and rockfish wholesale markets has positive 0.1059 value. Third, causality relation is unidirectional from rockfish market to flatfish market. Fourth, conditional volatility spillover effect is unidirectional from rockfish market to flatfish market, but asymmetric volatility effect is bidirectional between flatfish and rockfish markets that implies the bad news arising from flatfish wholesale market impact on rockfish market's volatility and the bad news arising from rockfish wholesale market impact on flatfish market's volaltilty. Consequently, based on the thus results, the volatility spillover effect interacts and is bidirectional between flatfish and rockfish wholesale markets.

• 한국수산과학회지
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• 제44권5호
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• pp.464-469
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• 2011

Residues of ampicillin and amoxicillin in the muscles of olive flounder Paralichthys olivaceus were investigated after oral doses of 20 mg/kg body weight/day of ampicillin and 40 mg/kg body weight/day of amoxicillin in a first examination and 40 mg/kg body weight/day of ampicillin and 80 mg/kg body weight/day of amoxicillin in a second examination for 5-6 days. The ranges of seawater temperatures in the $1^{st}$ and $2^{nd}$ examinations were $13.4-15.6^{\circ}C$ and $16.8-21.4^{\circ}C$, respectively. Ampicillin and amoxicillin concentrations were analyzed by high performance liquid chromatography (HPLC). The average recovery rates of ampicillin and amoxicillin in fish samples ranged from 84.8-95.2% and 100.8-103.8%, respectively. Residual concentrations in the olive flounder peaked on the $5^{th}$ day, with average concentrations of 0.075 mg/kg and 0.311 mg/kg in the $1^{st}$ examination, and 0.098 mg/kg and 0.630 mg/kg in the $2^{nd}$ examination for ampicillin and amoxicillin, respectively, with maximum concentrations in muscle. In the $1^{st}$ examination, ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentrations after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. In the $2^{nd}$ examination, the ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentration after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. We suggest that the recommended withdrawal periods should be 3 days for ampicillin and 10 days for amoxicillin in the olive flounder.

• 한국수산과학회지
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• 제56권5호
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• pp.606-614
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• 2023

This study was conducted to optimize the size of olive flounder Paralichthys olivaceus (OF) as a material of sikhae and to investigate the quality characteristics. The results on the protease activity of OF meat, protein and ash contents of the bone, and yields and hardness of fish bone during fermentation time suggest that the suitable fish weight for sikhae was less than 250 g. The proximate compositions of the OF sikhae fermented under optimum condition (fermentation for 9 days at 15℃), were 73.0% moisture, 12.0% crude protein, 1.3% crude fat and 2.4% ash. The salinity, titration acidity and amino acid nitrogen contents per 100 g sikhae were 1.7 g, 2.46 g, and 311.3 mg, respectively. The lactic acid bacteria concentration in the sikhae were 8.84 log CFU/g, which were higher than those (5.78-6.62 log CFU/g) of 5 kind of commercial flounder sikhae. The functional properties, such as ACE inhibitory activity (69.0%), antioxidative activity (69.3%), α-glucosidase inhibitory activity (22.7%), xanthine oxidase inhibitory activity (88.2%), and nitrite scavenging activity (96.4%) of the sikhae were superior to those of 5 kind of commercial flounder sikhae.

• 한국어병학회지
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• 제32권1호
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• pp.29-35
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• 2019

2015년부터 2017년까지 우리나라 넙치 양식장에서 발생하는 폐사피해를 표본조사를 통해 모니터링 하였다. 표본어가는 전국의 수산질병관리원과 지속적으로 거래하고 있으며 현장방문을 통해 설문이 가능한 양식어가 중 층화구조분석을 통해 선정 하였다. 수산질병관리사 조사요원의 직접적인 현장방문을 통한 설문 조사를 수행하여 객관적으로 양식현황 및 폐사 현황을 파악하였다. 조사기간 중 24.78%/2015년, 30.19%/2016년 그리고 21.59%/2017년의 누적 폐사율을 나타내었고, 감염성 질병에 의한 폐사는 스쿠티카증(2015-2017년 평균 누적 폐사율: 56.7%)과 바이러스성출혈성 패혈증 감염(추정)(2015-2017년 평균 누적 폐사율: 8.9%)에 의한 피해가 높은 것으로 나타났으며, 제주에서는 여윔증(2016-2017년 평균 누적 폐사율: 10.3%)에 의한 피해가 높게 나타났다.

• 생명과학회지
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• 제19권1호
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• pp.87-93
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• 2009

양식 어류의 항생제 투여의 문제점을 해결하기 위하여 한 약재 추출물을 기질로 한 유산균 배양액을 양식 넙치와 돌돔의 사료에 첨가하여 성장, 혈액학적 분석, 식세포 활성, lysozyme 활성 및 어류질병세균에 대한 공격실험을 실시하였다. 성장의 결과 넙치는 실험구와 대조구강의 유의성을 보이지 않았으며, 돌돔의 경우 대조구에 비해 실험구가 약 20 g 높은 어체중 증가를 보였다. 넙치의 사료효율은 실행구가 대조구에 비해 약 25% 높았으며, 돌돔은 사료효율은 두 그룹 간에 유의성을 보이지 않았다. 어류의 혈액학적 분석 결과, GOT와 GPT는 8주 이후에 감소현상을 보였으나 glucose와 total protein은 두 그룹 간에 유의성을 보이지 않았다. 식세포 활성 또한 유의적인 차이가 없었다. lysozyme 활성은 넙치의 경우 8주부터 실험구가 대조구보다 높은 활성을 보였으며, 돌돔은 실험 12주에 대조구보다 약 3배 높은 활성을 보였다. 어류 질병 유발세균에 대한 공격실험은 넙치의 복강에 V. anguillarum을 투여하였을 시, 실험구가 대조구에 비해 18%, S. iniae을 투여하였을 시, 실험구가 23% 높은 생존율을 보였다. 돌돔의 경우, V. anguillarum의 투여경과 대조구의 비해 17%, S. iniae의 투여경과 대조구의 비해 16% 높은 생존율을 나타냈다.

• 수산해양교육연구
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• 제27권6호
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• pp.1610-1618
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• 2015

This study is aimed to estimate price interactions between wild caught fish and farmed fish on the Korean seafood market, using multivariate cointegration analysis. Major commercially important four wild caught fish (chub mackerel, hairtail, yellow croaker and spanish mackerel) and two farmed fish(oliver flounder and black rockfish) are selected as analytical target fish species. Between 2000 and 2013, monthly production and price data are used in this study. The results of cointegration test showed that there would be a long-term equilibrium relationship among 4 wild caught fish and 2 farmed fish. However, the results of exclusion test indicated that farmed fish might not contribute significantly to the long-run relationship, suggesting that farmed fish might be only a weak substitute for wild caught fish, but no significant interaction could be found.

• 수산경영론집
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• 제45권2호
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• pp.85-95
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• 2014

Fish stocking is important element of land-based aquaculture management. To maintain constant stocking rate considering biological and economic condition is a convenient strategy in intensive aquaculture. This study is aimed to analyze the effect of over-stocking(more than aquaculture capacity) for certain periods of time. This study make the mathematical decision making model that finds the value of decision variable to minimize cost that sums up the water pool usage cost and sorting cost under critical standing corp constraint. The proposed mathematical decision making model was applied to 12 sample combination of sorting cost and the number of fish on the Oliver flounder culture farms. If a immature fish can be sold for high price than farming cost, restricted over-stocking resulted in a improvement of economic performance. When extensive comparable biological and market data become available, analysis model can be widely applied to yield more accurate results.

• 한국양식학회지
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• 제16권2호
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• pp.104-109
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• 2003

The present study aims to evaluate protein and phosphorus availabilities of five different dietary protein sources during the 6-week feeding trial in juvenile olive flounder, Paralichthys olivaceus as determined by growth performance and phosphorus retention. Five diets containing blood meal (BM), poultry by-product (PBP), squid liver powder (SLP), feather meal (FM) and soybean meal (SM) were prepared by mixing a basal diet (BD) with one of five test ingredients at the ratio of 7 to 3. As a reference diet, BD contains three different protein sources such as white fish meal, casein and gelatin. After 2 weeks of the conditioning period, fish initially averaging 2.7$\pm$0.02g (mean$\pm$SD) were randomly distributed into each aquarium as a group of 30 fish reared in the recirculating system. Fish of triplicate groups were fed one of six experimental diets (BD+five test diet). After 6-week feeding trial, pro- tein efficency ratio (PER) of fish fed BM diet was the lowest in experiment groups. While fish fed PBP diet showed a significant higher PER as compared to the FM diet, and fish fed SLP diet and BD were a higher PER than did fish fed PBP diet. However, there was no significant difference in PER among fish fed SLP diet, BD and SM diet, and between SM diet and PBP diet. Phosphorus retention efficiency of bone (PR $E_{b}$) of fish fed BM diet was the lowest in all the diets, and fish fed FM diet showed a higher PE $R_{b}$ than fish fed BD and SM diet. However, there was no significant difference in PER among fish fed FM diet, SLP diet and PBP diet, and among SLP diet, PBP diet, SM diet and BD. These results indicate that SLP could be a suitable protein source for low pollution diets of olive flounder in the future fish feeds market. Furthermore, PBP and SM are available protein source to reduce P waste in the oliver flounder aquaculture with the use of proper mixture of other protein sources and more processing to improve protein availibility of these.ese.

• 수산해양교육연구
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• 제28권3호
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• pp.611-618
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• 2016

A $3{\times}2$ factorial experiment was conducted to investigate effects of water temperature and feeding rate on growth and body composition of olive flounder. Triplicate groups of fish (initial body weight of 118 g) fed a extruded pellet (55% protein and 4708 cal/g) to satiation and at restricted feeding rates of 0.25 and 0.4% body weight per day (BW/d) at the different water temperatures (13 and $18^{\circ}C$) for 9 weeks. Weight gain increased significantly with increase in feeding rates at each temperature. Weight gain of fish fed to satiation was significantly higher at 18 than $13^{\circ}C$, whereas, that of fish fed at 0.25 and 0.4% BW/d were significantly or slightly lower at 18 than $13^{\circ}C$. Feed efficiency and protein efficiency ratio of fish fed to satiation were not significantly different between 13 and $18^{\circ}C$, but those of fish fed at 0.25 and 0.4% BW/d were significantly higher at 13 than $18^{\circ}C$. The major finding of this study is that satiation feeding is efficient for optimal growth and feed efficiency of grower oliver flounder (116-164 g) in suboptimal water temperatures. The maintenance feeding ration which is zero growth performance, were 0.30 and 0.41% BW/day at 13 and $18^{\circ}C$, respectively. In the restricted feeding regime, compromised growth of fish were worsen in higher water temperature ($18^{\circ}C$ vs. $13^{\circ}C$). It might be related to high metabolic rate of fish that spend more energy for maintenance metabolism. Based on these results, we suggest that a satiation feeding regime is recommended for a productive growth of grower olive flounder in the suboptimal temperature.