Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of the Culture Broth of Lactic Acid Bacteria Cultured in Herb Extracts on Growth Promotion and Nonspecific Immune Responses of Aquacultured Fish

한약재 추출물에 배양된 유산균 배양액이 양식어류의 사료첨가제로서 성장과 비 특이적 면역에 미치는 영향

  • Jhon, Bong-Kun (Department of Aquatic Life Medicine, Cheju National University) ;
  • Kim, Man-Chul (Department of Aquatic Life Medicine, Cheju National University) ;
  • Kim, Young-Hoo (Jewoo corporation LTD.) ;
  • Heo, Moon-Soo (Department of Aquatic Life Medicine, Cheju National University)
  • 전봉근 (제주대학교 해양과학대학 수산생명의학과) ;
  • 김만철 (제주대학교 해양과학대학 수산생명의학과) ;
  • 김영후 ((주)제우축산연구소) ;
  • 허문수 (제주대학교 해양과학대학 수산생명의학과)
  • Published : 2009.01.30
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Abstract

We have investigated the effects of the culture broth of lactic acid bacteria (LAB) cultured in herb extract on growth, hematological parameter, nonspecific immune responses and disease resistance of olive flounder (Paralichthys olivacells) and parrot fish (Oplegnathus fasciatus) for 12 weeks. Weight gain of olive flounder fed diet with mixture was not significant among the control group. But In parrot fish, was significantly higher 20g than control group. The feed efficiency of olive flounder were 25% higher in the experimental groups than in the control. There were no significant differences in feed efficiency among each group on parrot fish. Treatment of olive flounder contents of GOT and GPT in serum decreased after 8 weeks. But there were no significant differences in GLU and TP among each group. Also, there was no significant of NBT reduction. The activities of lysozyme were higher in experimental group of olive flounder than in the control after 8 weeks. On the other hand, activities of lysozyme were triple higher in the experimental group of parrot fish than in the control after 12 weeks. In the oliver flounder case, the survival rate (%) after an artificial challenge with $10^7$ CFU/ml of Vibrio anguillarum and Streptococcus iniae per fish, was 18% higher in the experimental groups than the control. The higher survival rate of parrot fish were 17% and 16% in the experimental groups than the control respectively.

양식 어류의 항생제 투여의 문제점을 해결하기 위하여 한 약재 추출물을 기질로 한 유산균 배양액을 양식 넙치와 돌돔의 사료에 첨가하여 성장, 혈액학적 분석, 식세포 활성, lysozyme 활성 및 어류질병세균에 대한 공격실험을 실시하였다. 성장의 결과 넙치는 실험구와 대조구강의 유의성을 보이지 않았으며, 돌돔의 경우 대조구에 비해 실험구가 약 20 g 높은 어체중 증가를 보였다. 넙치의 사료효율은 실행구가 대조구에 비해 약 25% 높았으며, 돌돔은 사료효율은 두 그룹 간에 유의성을 보이지 않았다. 어류의 혈액학적 분석 결과, GOT와 GPT는 8주 이후에 감소현상을 보였으나 glucose와 total protein은 두 그룹 간에 유의성을 보이지 않았다. 식세포 활성 또한 유의적인 차이가 없었다. lysozyme 활성은 넙치의 경우 8주부터 실험구가 대조구보다 높은 활성을 보였으며, 돌돔은 실험 12주에 대조구보다 약 3배 높은 활성을 보였다. 어류 질병 유발세균에 대한 공격실험은 넙치의 복강에 V. anguillarum을 투여하였을 시, 실험구가 대조구에 비해 18%, S. iniae을 투여하였을 시, 실험구가 23% 높은 생존율을 보였다. 돌돔의 경우, V. anguillarum의 투여경과 대조구의 비해 17%, S. iniae의 투여경과 대조구의 비해 16% 높은 생존율을 나타냈다.

Keywords

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