• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.227-233
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• 2000

Preset study was carried out to evaluate characteristics of lactic acid producing bacteria(LAB) in hen's cecum as probiotics value. Distribution of LAB in intestinal tracts was investigated using 5∼25 weeks - old hens. So, 12 strains to LAB with different morphology were isolated purely. Acid tolerance of LAB tested at pH 1, 2, 3, and 4, and bile resistant also tested at 0, 0.3% and 0.5% bile salt concentration. Growth pattern of LAB observed to 60h. All strains of cecal LAB couldn't survive at pH 1, and decreased linearly survival colony after incubation at pH 2 although some strains could survive for 2h. Most of LAB maintained constant number at pH 3 and 4. The bacterial action could increase linearly at 0% bile salt concentration in all of tested strains. However, only one strain could multiply at 0.3% bile salt, others were influenced by bile salt. That tendency was similar at 0.5% bile salt. Growth was peaked at 12 to 18 h after innoculation. After peak, the decreasing pattern of colony was different to strains which some strains decreased rapidly or maintained for long time. The LAB of hen's cecum was similar to intolerance acidity, but different to resistant to bile salt and growth pattern by strain. So, we choose three strains which have probiocs value, and identified as Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9 and Lactobacillus vaginalis LLA11.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.11
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• pp.1605-1609
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• 2001

A total of 252, 50 week-old Isa-brown laying hens were randomly allotted to 7 groups of 3 replicates. Mustard meal (MM) which is a by-product from mustard processing plant, was dried under the sun or in a gas heated pan. It contained on DM basis 30-32% CP, 19-22% EE and 12-13% CF. The meal from either drying method was incorporated into the diets at 0, 10, 20 and 30% which was equivalent to the substitution levels for soybean meal at 0, 31, 63 and 94%, respectively. All birds were individually kept in battery cages where feed and water were freely accessed throughout 84 days experimental period. It was found that egg production, feed intake, body weight gain and egg weight significantly decreased with the increased MM level. The inclusion of 20% MM did not show a significant difference in egg production and quality from the control, but produced 6-8% lower egg production. Feed intake was linearly decreased with the MM levels, except the 10% sun dried MM group. Fat deposition of the birds fed MM diets significantly decreased, while kidney weight increased when compared with the control group. However, the weight of thyroid glands and spleen trended to be heavier in the MM groups, but this was not significantly different among dietary treatments. It was concluded, MM from both drying methods could be incorporated in laying hen diets at the level of 10% without any adverse effect.

• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.171-176
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• 2002

Two experiments were conducted to investigate the effects of taurine supplementation on the egg production and egg quality in laying hen. In experiment 1, 19-wk-old laying hens were allowed one of four taurine supplemented diets (0 (control), 0.4, 0.8, and 1.2% taurine) for 10 weeks. The mean egg weight was lower about 1.2g in the 0.8% diet compared to the control. Egg production rate, egg mass, and feed intake in layers fed with 0.4, 0.8, and 1.2% taurine supplemented diets decreased by 8%∼24%, 3%∼l4% and 11%∼l5%, respectively, relative to the control. However, the feed conversion, parameters of egg quality, and the concentration of triacylglycerol and the level of cholesterol in yolk were not different among the treatments. In experiment 2, 81-wk-old laying hens were allocated one of three taurine supplemented diets(0 (control), 1 and 2% taurine) for 6 weeks. The egg weight, egg production rate, egg mass, feed intake, feed conversion, parameters of egg quality, and level of yolk cholesterol were not different among the treatments, but the concentration of triacylglycerol in yolk was higher by 14%∼19% compared to the control. In conclusion, these results indicated that taurine supplementation in early laying stage of layers decreased hen performance.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.421-426
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• 2009

This study was conducted to clarify the characteristics of vocalization of laying hen related with space in battery cage. The size of cages were classified into control (0.30 m ${\times}$ 0.14 m ${\times}$ 0.55 m, length ${\times}$ width ${\times}$ height), small (0.21 m ${\times}$ 0.14 m ${\times}$ 0.55 m) and large (0.30 m ${\times}$ 0.30 m ${\times}$ 0.55 m) size. Vocalization of 16 individuals of laying hen in each group of Hy-Line Brown (80 week old) were recorded 3 hours per day (10:00am~11:00am, 3:00pm~4:00pm and 7:00pm~8:00pm) using digital recorder and microphone during October 2008 and February 2009. Characteristics of frequency, intensity and duration of vocalization were analyzed by GLM (general linear model) and Duncan's multi-test. There were differences in basic and maximum frequency, and intensity based on analysis of spectrogram and spectrum among different cage sizes. Vocalization of laying hen would be one of the indicators to understand the stress caused by rearing space in batter cage.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1464-1469
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• 2016

This study was carried out to investigate the effect of dietary supplementation of red ginseng by-product (RGB) on the laying performance, blood biochemistry, and microbial population in laying hens. A total of 120 Hy-Line Brown laying hens (75 weeks old) were randomly allotted to 1 of 3 dietary treatments with 4 replicates per treatment. A commercial-type basal diet was prepared, and 2 additional diets were prepared by supplementing 5.0 or 10.0 g/kg of RGB to the basal diet at the expense of corn. The diets were fed to hens on an ad libitum basis for 4 weeks. There were no differences in feed intake, egg weight, and feed conversion ratio during 4 weeks of the feeding trial. However, hen-day egg production was significantly greater (p<0.05) for the RGB treatment groups than that for the basal treatment group. There were no differences in triglyceride, aspartate aminotransferase, and alanine aminotransferase during the 4-week feeding trial. However, RGB supplementation increased (p<0.05) the serum immunoglobulin G (IgG) and IgM content compared with basal treatment group. The total cholesterol was lower (p<0.05) in the RGB treatments groups than that in the basal treatment group. The intestinal Lactobacillus population was greater (p<0.05) for the RGB treatments groups than that for the basal treatment group. However, the numbers of Salmonella and Escherichia coli were not different among dietary treatments. During the entire experiment, there was no significant difference in egg quality among all the treatments. In conclusion, in addition to improving hen-day production, there were positive effects of dietary RGB supplementation on serum immunoglobulin and cholesterol levels in laying hens.

• Korean Journal of Poultry Science
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• v.15 no.4
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• pp.281-286
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• 1988

This experiment was conducted to determine the optimum level of Red Pepper (including seeds) for laying-hen'diet in summer. A total 240 birds of 37 week-old Manina Brown Hen was alloted to 4 treatments, 3 replications per treatment, according to complecately randomized design to find out the effects of adding levels (0, 0.03, 0.05, 0.08%) of Red Pepper on laying Performance and e99 Quality of experimental birds for 10 weeks. The results obtained from this study were summaried as follows : 1) I班 shell thickness was different significantly among treatments, (P＜0.05). Especially, Ta was highest one. 2) Egg shell breaking strength was highly significant difference between C and $T_1$, $T_2$, $T_3$ (p＜0.01). 3) Yolk color of $T_1$, $T_2$, $T_3$ was higher than C (p＜0.01), but there was no significant difference between treatments. 4) Egg Production was slightly higher with adding Red Pepper, but the differences were no significant. 5) No significant differences were found in average egg weight among treatments.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.291-298
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• 2023

The purpose of this experiment was to determine the influence of providing laying hens with extra feed on egg production and egg quality parameters. A total of 480 laying hens (38-weeks old), were divided into five treatment groups (eight replicate cages/treatment and 12 layer/replicate) according to their starting body weight (1.98 ± 0.05 kg) in this four-week feeding trial. Five different feed allowances of the same diet (105, 110, 115, 120, and 125 g·day^-1·bird^-1) were assigned to layers. Daily inspections of remaining feed (around 0.1g) and layer mortality (0%) showed no harmful impact of supplying extra feed to layers. Providing 120 and 125 g of feed per day to layers resulted in the highest final body weight, large-egg ratio, and improved yolk color among all treatment groups. Layers receiving 125 g of feed daily had the highest egg weight, but the highest egg production ratio was observed in layers receiving 110 g of feed/day. The additional supply of feed did not have a negative impact on the productive performance or egg quality of the layers. The provision of 125 g feed per day led to an improvement of large-egg ratio, egg weight, and yolk color, but likely led to obesity of the layers, which manifested as an increase in body weight and a decline in the egg production ratio. We concluded that 110 grams of feed was the proper quantity after taking into consideration the significance of the health of the laying hen to the overall production performance.

• Korean Journal of Veterinary Research
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• v.48 no.4
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• pp.469-472
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• 2008

Necropsy was performed on a total of twenty three either white leghorn or ogol chickens which were more than 150 weeks of age. Among twenty three chickens examined, fifteen chickens were laying and the rest eight chickens were non-laying. On necropsy, neoplastic mass in the five chickens among non-laying chickens was found. These neoplastic masses were present mostly in the ovaries and one case in the liver and characterized by multifocal to coalescing 1 to 5 mm tan firm nodular formation. On histopathology, ovarian adenocarcinoma with widespread abdominal seeding and hepatic metastasis was diagnosed in the three chickens. Oviductal leiomyoma was also found in two chickens that had a focal well-demarcated nodules in the oviduct. Taken together, the number with ovarian adenocarcinoma among non-laying chickens over 150 week old was 37.5%. As most animal species do rarely develop ovarian tumors, the high rate of spontaneous ovarian adenocarcinoma in non-laying hen suggest that the hen is a proper model for human ovarian cancer study.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.8
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• pp.1174-1178
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• 2006

An experiment was conducted to investigate the effects of dietary supplementation of copper chelates in the form of methionine, chitosan and yeast on the performance of laying hens. Four hundred ISA Brown layers, 84 wks old, were assigned to 4 treatments: control, 100 ppm Cu in methionine chelate (Met-Cu), 100 ppm Cu as chitosan chelate (Chitosan-Cu) and 100 ppm Cu as yeast chelate (Yeast-Cu). Each treatment had five replicates of 20 hens. Hen-day and hen-housed egg production and egg weight were significantly (p<0.05) increased by Met-Cu supplementation. The increase by Chitosan-Cu and Yeast-Cu supplementation was not significant. Contrast of the control vs. Cu chelates showed egg weight was significantly (p<0.05) increased by Cu chelate supplementation. Soft-shell egg production was significantly (p<0.05) reduced by supplementation of Cu chelates. Met-Cu treatment showed the lowest incidence of soft egg production. Gizzard erosion index was increased by Cu chelate supplementation. Crude fat in liver, total cholesterol in yolk and Cu content in liver and yolk were not significantly influenced by Cu chelate supplementation. It was concluded that dietary supplementation of 100 ppm Cu as Met-Cu significantly increased egg production and egg weight. Cu-Met chelate was also effective in reducing soft-shell egg production but increased gizzard erosion index.