• 한국발생생물학회지:발생과생식
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• 제10권2호
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• pp.135-140
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• 2006

소형 두족류의 일종인 낙지(Octopus minor)는 근연종인 문어나 주꾸미에 비하여 산란수가 적고 갯벌 속 깊은 은신처에서 어미 낙지의 독특한 포란 행동으로 부화하기 때문에 배의 발달 과정을 관찰하기가 매우 어렵다. 본 연구에서는 인공 산란관을 설치하여 낙지의 산란과 부화를 유도하고 배 발달과정을 관찰하였다. 낙지의 배아에서는 산란 후 4일째에 배반엽이 형성되었으며 28일이 경과하면 여러 기관의 원기들이 난황낭의 중앙부에 출현하였다. 이 발생 원기는 동물극 쪽으로 이동하여 38일이 경과되면 두부에 두 눈이 생기고 몸통과 심장이 난황낭의 정단부에 출현하였다. 45일이 경과 된 뒤에는 몸통과 두부가 난막의 절반을 차지하게 자라고 길고 가느다란 팔을 움직이기 시작하였다. 산란 후 60일이 경과 한 뒤에는 여러 개의 반점을 갖는 몸통의 아래에 2개의 눈이 뚜렷하게 형성되었고 80일이 지나면 난황은 거의 흡수되고 그 자리에는 잘 발달된 8개의 팔이 가득 채우게 되었다. 발생이 끝난 새끼 낙지는 알 껍질을 찢고 팔부터 빠져 나왔으며 부화 후 곧바로 활발하게 유영하며 먹물을 분사하였다.

• Applied Microscopy
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• 제32권1호
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• pp.17-30
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• 2002

살오징어 (Todarodes pacif icus)와 서해낙지 (Octopus minor)의 망막은 외절부, 간상기저부, 내절부 그리고 망상층 등 4개의 층으로 구분되었고, 망막의 두께는 오징어 ($385{\sim}400{\mu}m$)에서보다 낙지 ($400{\sim}420{\mu}m$)에서 $20{\mu}m$ 정도 더 두터웠다. 망막은 시각세포와 지지세포로 구성되어 있는데, 서해낙지의 지지세포 상단에는 미세융모 (길이 $0.6{\sim}0.7{\mu}m$)가 밀생된데 비해 살오징어에서는 확인되지 않았다. 망막을 구성하는 시각세포와 지지세포들은 광선을 차단하는 색소과립들을 소지하고 있었는데, 살오징어인 경우 시각세포의 색소과립(크기, $2.0{\times}0.5{\mu}m$ 정도)이 지지세포의 색소과립 (크기, $1.0{\times}0.3{\mu}m$ 정도)에 비해 컸으나, 서해낙지에서는 두 세포간 색소과립의 크기 ($0.8{\times}0.6{\mu}m$ 정도)가 비슷하였다. 시각세포의 세포질 상단부에서 빗살모양의 미세융모들이 정육각형 구조 (직경, 60 nm)인 rhabdome을 형성하였는데 한 개의 rhabdome은 4개의 rhabdomere로 구성되어 있으며, 전체 rhabdome의 총 단면적은 서해낙지가 살오징어에 비해 두 배정도 컸다. 망상층을 구성하는 연접체는 살오징어인 경우 전자밀도가 높은 핵을 포함하는 소포 (electron dense-core vesicle)와 전자밀도가 낮은 소포 (electron lucent vesicle)들로만 형성된 연접체 등 두 종류를 소지한 반면, 서해낙지에서는 전자밀도가 높은 소포(electron dense vesicle)와 전자밀도가 낮은 소포들이 혼합된 형과 전자밀도가 낮은 소포들로만 이루어진 연접체 등 두 종류를 소지하고 있어 두 종간 차이가 확인되었다.

• 수산해양기술연구
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• 제43권4호
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• pp.291-300
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• 2007

The crayfish-shaped artificial bait for octopus drift line is manufactured, into which is made to insert ordinary bait. The effects of the artificial bait are confirmed through the analysis of octopus' behavior in the laboratory in comparison with the existing bait of pig-fat skin, and the investigation of the octopus catch on the sea, and in addition, the examination of the water quality in relation to the bait to be inserted into the artificial bait is done together. The artificial bait is red in color, 10.5cm in the length of the body, and 29g in weight. The octopus behavior in relation to the bait in the laboratory has shown a good result in which more than 30% of the octopuses rushed to the artificial bait. In the analysis of the octopus sitting time by the bait, the octopus stayed longer with the artificial bait(63.7%) than the pig-fat skin(25.1%). The octopus sitting time at the artificial bait inserted with frozen squid(48.8％, 44.6％) is shown to be longer than that of the pig-fat skin(36.9％) or boiled fish paste(21.2％). In the analysis of sitting frequency to the bait, the case of the artificial bait(total 17 times) was more than that of the pig-fat skin(total 3 times), and the case of the artificial bait with frozen squid attracted the octopuses more frequently than that of the pig-fat skin or the boiled fish paste. In the field experiment, the fishing boat A(Manseon-ho, 1.22tons) caught the total 93 octopuses, while the fishing boat B(Ilho-ho, 0.73tons) caught the total 154 octopuses, all of which weighed less than 9.0kg. In the comparison with the total catch, the case with the artificial bait was a little higher than that of the pig-fat skin($^{**}p\;<\;0.05$). In the analysis of the water quality in relation to the baits, the COD showed the descending order of frozen squid(0.57mg/g), boiled fish paste(0.18mg/g), and pig-fat skin(0.10mg/g), and the case with frozen squid was the highest and the case of the pig-fat skin, lowest. The total phosphorus, like the COD, showed the highest in frozen squid(0.02mg/g), and in case of the total nitrogen, unlike the COD, the pig-fat skin was shown to be the highest(0.006mg/g).

• 수산해양기술연구
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• 제51권1호
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• pp.1-8
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• 2015

The common octopus, which has a high growth rate and high market price, is a prime candidate for commercial marine aquaculture operations. We evaluated the effectiveness of two types of shelters (pipe and tire types) for juvenile common octopus growing out in flow-through seawater tanks. Growth rates were evaluated in two experiments. The first experiment (Experiment 1) ran for 72 days, and the second (Experiment 2; replicated) ran for 46 days. Each trial included 40 octopuses fed a diet of frozen sardine (Sardinops melanostictus) and swimming crab (Portunustri tuberculatus) at 3-8% of body weight once every 3 days. In the two experiments, the respective specific growth rates were 0.3 and 0.04%/day in pipe-type shelters and 0.00 and 0.88%/day in tire-type shelters, while the respective percentage survivals were 80 and 80% in pipe-type shelters and 70 and 90% in tire-type shelters. Shelter type had little influence on the growth rate (P < 0.05).

• 수산해양기술연구
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• 제43권3호
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• pp.176-182
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• 2007

Fishing power, which means performance of fishing vessel or catchability of fishing gear, can explain using by fishing power index(FPI) to compare fishery efficiency among uniformity types of fishery that work during the fixed period in specific fishing ground. This research analyzed on their fishing power and catchability using comparing each sampled vessels of coastal trap fishery for common octopus. The results showed that they were no difference in amount of used trap and immersed time etc. in CPUE among sampled vessels, and had no correlation of catch production due to vessel's tonnage. Most vessel's FPI estimates but 3 vessels were higher than the averaged, and showed similar fishing power in general. And then, CPUE and FPI showed that 4 to 5 tonnage vessels would be superior to another, 4 tonnage vessels had also good catchability. Therefore, we estimated that 4 tonnage vessels had the most efficiency work for coastal trap fishery for common octopus.

• 수산해양기술연구
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• 제26권3호
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• pp.237-243
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• 1990

본 연구는 수조실험을 통하여 주꾸미, Octopus Ocellatus GRAY의 단지색에 대한 선택성을 조사하고, 전통적인 주꾸미 단지의 재질을 고둥껍질에서 PVC로 대체할 경우 그 가능성을 규명하기 위하여 PVC 시험 단지를 제작하여 1989년부터 1990년까지 해상실험을 실시하여 어획율을 조사하였다. 실험에서 얻은 결과를 요약하면 다음과 같다. 1. 수중실험에서 적색, 녹색, 황색, 백색, 회색중에서 주꾸미가 선호하는 색은 회색, 적색, 녹색, 황색, 백색순으로 나타났다. 2. 해상실험에서 주꾸미의 선호색은 회색, 홍색, 녹색순으로 나타났고, 이것은 수조실험의 결과와 일치한다. 3. 어획성능면에서 고둥껍질과 회색 PVC 단지의 차이는 거의 없었다. 4. 직경별 어획율은 40mm PVC 단지가 1.00, 50mm가 0.19로 나타났다.

• 한국수산과학회지
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• 제40권4호
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• pp.243-247
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• 2007

We investigated the reproductive behavior of Octopus minor, order Octopoda, class Cephalopoda under laboratory conditions. Each mature female octopus was kept in an aquarium with a plastic tube for shelter, and one mature male was introduced for the purpose of copulation. Before spawning, the female coated the roof of the shelter with a light-green material, upon which it then fixed its eggs one by one. This spawning behavior lasted 1 to 3 days. Fertilized females spawned 54 eggs on average, ranging from 21 to 112 eggs at 72 to 98 days after copulation. The attached eggs were 18.1-19.0 mm in length, 5.0-6.1 mm in width, and 0.30-0.38 g in weight. The mother octopods did not feed; they attended to the eggs by using their arms to rub the egg surfaces and used their funnel to blow sediments off of the eggs. At water temperatures of $20.9-21.5^{\circ}C$, the fertilized eggs hatched within 73 to 90 days after being spawned. The effective cumulative water temperature was $1,569-1,892^{\circ}C$. At the end of incubation, the body weight of the mother octopods was reduced to approximately 56% of the initial weight, and most mother octopods died soon after the young hatched.

• 해양환경안전학회지
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• 제12권3호
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• pp.179-183
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• 2006

$CO_2$를 해양에 격리 처리하기 위해서는 $CO_2$가 해양생태계에 어떠한 영향을 미치는가에 대한 연구가 선행되어야 한다. 이 연구에서는 $CO_2$의 해양생물영향실험에 있어 연체동물의 이용가능성을 모색하기 위한 연구로, 먼저 문어에 적합한 마취농도를 확립하고, 혈액을 채취하기 위한 케뉴레이션 방법 및 문어가 케뉴라를 탈착시키는 것을 방지하기 위한 구속 방법에 대해서 검토하였다. 그 결과 1) 문어에 적합한 마취제는 5.25% $MgCl_2\;6H_2O$인 것으로 나타났다. 2) 문어로부터 혈액채취를 위한 케뉴라 삽입 부위는 배대동맥이 적합한 것으로 나타났다. 3) 케뉴라 보호를 위하여 플라스틱 넷트로 만든 구속 쳄버를 이용하는 것이 유용한 것으로 나타났다

• Fisheries and Aquatic Sciences
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• 제27권2호
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• pp.111-121
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• 2024

Particulate matter (PM) is a mixture of microscopic solid inhalable particles including airborne liquid droplets, and it is implicated with several diseases. The eye does not have a protective barrier among the human organs, consequently it get directly exposed to environmental substances such as PM. The scarcity of treatments for damage to the eyesight and the vision and eye structure being closely related to the structure and function of the central nervous system highlights the cruciality of novel therapeutics. In this study was conducted using in vivo zebrafish vertebrate model which is a useful model due to the conserved genes between human. We found that protective effect of Octopus minor extract against particulate matter-induced adverse effects on eye development in zebrafish (Danio rerio) embryos by regulating antioxidant and anti-inflammatory mRNA expression.

• 수산해양기술연구
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• 제41권4호
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• pp.271-278
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• 2005

In order to study the catch situation of the octopus pot fishery in the coastal waters of Gangneung, catch quantities and weight per month and depth, the soaking time & loss of fishing gear were studied based on 62 fishing times (1 set consisted of 150 pots which is equivalent to 1 fishing time) over a period of 10 months from February to December 2003 using commercial fishing vessels and training ships. The monthly CPUE was generally high in the months of june - September, and during this period July showed the highest quantity (134g/pot, 19.21kg/haul). In consideration of the fact that octopus below the weight of 2kg is protected in foreign countries, the majority of the catch was small with 76% (486 octopuses) below 1kg. The octopus catch quantity was high in depth of more than 40m, and the average catch number per set of pots in depths of over 50m was the highest with 13 octopuses. CPUE(g/pot) was the highest at a depth of over 60m recording 101. In terms of catch quantity per soaking time (4-18 days) the highest was on the 7th day, and 13-18 days also recorded high catches, and this suggests the possibility of ghost - fishing with the loss of fishing gear. Loss of fishing gear was the highest in terms of loss frequency and amount at depths of 20-30m. Consequently, it seems that octopus pot fishing at depths of more than 30m would relieve friction with other types of fishing and increase fishing efficiency.