Knock-out of a gene by insertional mutagenesis is a direct way to address its function through the mutant phenotype. Among ca. 15,000 gene-trapped Ds insertion lines of rice, we identified one line from selected sensitive lines in highly salt stress. We conducted gene tagging by TAIL-PCR, and DNA gel blot analysis from salt sensitive mutant. A gene encoding an oligopeptide transporter (OPT family) homologue was disrupted by the insertion of a Ds transposon into the OsOPT10 gene that was located shot arm of chromosome 8. The OsOPT10 gene (NP_001062118.) has 6 exons and encodes a protein (752 aa) containing the OPT family domain. RT-PCR analysis showed that the expression of OsOPT10 gene was rapidly and strongly induced by stresses such as high-salinity (250 mM), osmotic, drought, $100\;{\mu}M$ ABA. The subcellular localization assay indicated that OsOPT10 was localized specifically in the plasma membrane. Overexpression of OsOPT10 in Arabidopsis thaliana and rice conferred tolerance of transgenic plants to salt stress. Further we found expression levels of some stress related genes were inhibited in OsOPT10 transgenic plants. These results suggested that OsOPT10 might play crucial but differential roles in plant responses to various abiotic stresses.
To improve the growth inhibition activities and selectivities for quinclorac family, novel 3-substituted phenyl-5-(3,7-dichloro-8-quinolinyl)-1,2,4-oxadiazole derivatives as the substrate were synthesized and their the activities ($pI_{50}$) against shoot and root of rice plant (Oryza sativa L.) and barn-yard grass (Echinochloa crus-galli) were measured. And the quantitative structure-activity relationships (QSARs) between physicochemical parameters of the substitutents (R) on phenyl group and the activities ($pI_{50}$) were analyzed quantitatively. According to the SAR analyses, the substrates of planar conformation showed higher herbicidal activities against barnyard grass than rice plant. The activities against rice plant depend on the electronic effect (shoots: ${\sigma}_{opt.}=0.49$ & root: $R_{opt.}=-0.15$) of substituents, whereas the activities against shoots and roots of barnyard grass depend on hydrophobicity (${\pi}_{opt.}=0.37{\sim}2.40$). There were conditions of selective growth inhibition activity against barnyard grass when such a ortho-substituted electron donating substituents showing the hydrophobicity value, ${\pi}=2.40$ were introduced on the phenyl ring. The 2-tolyl substituent predicted from SAR equations was expected to have better growth inhibition activity and selectivity (${\Delta}pI_{50}=1.26$) for barnyard grass.
This study was to explore factors determining school-based student health screening participation. Participants were six health coordinators in West Virginia, the United States. They have responsibilities in the student health screening and conducted a phone interview. The results are as follows. First, opt-in of health screening came from the interest in students' health, peer pressure, and free screening. Second, opt-out of health screening came from the indifference to a school newsletter, anxiety about an information disclosure, and the fear of drawing blood. Third, the advantage of health screening was provided for free. Fourth, the primary decision-maker was parents, but sometimes was children. Fifth, non-family members also influenced on the participation in the health screening. Therefore, a school newsletter, parents and teachers education, and free screening may be required to improve the participation rate.
Korean Journal of Air-Conditioning and Refrigeration Engineering
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v.29
no.12
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pp.638-644
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2017
Optimal cooling operation algorithm was developed based on a simulation case of a single family house model equipped with renewable energy facility. EnergyPlus simulation results were used as virtual test data. The model contained three energy storage elements: thermal heat capacity of the living room, chilled water storage tank, and battery. Their charging and discharging schedules were optimized so that daily electricity bill became minimal. As an optimization tool, linear programming was considered because it was possible to obtain results in real time. For its adoption, EnergyPlus-based house model had to be linearly approximated. Results of this study revealed that dynamic cooling load of the living room could be approximated by a linear RC model. Scheduling based on the linear programming was then compared to that by a nonlinear optimization algorithm which was made using GenOpt developed by a national lab in USA. They showed quite similar performances. Therefore, linear programming can be a practical solution to optimal operation scheduling if linear dynamic models are tuned to simulate their real equivalents with reasonable accuracy.
Responsible tourism has become an established area of the tourism industry. This study aims to identify the factors that influence re-participation in responsible tourism in rural Korea. On-site survey was conducted on 436 tourists by seven responsible tourism agencies in Korea. The motivation for responsible tourists was categorized into seven types: family togetherness, escape and relaxation, personal growth, social interaction, various experiences, learning, and natural experience. The estimation of a binary logistic regression model determined the characteristics of responsible tourists who are most likely to opt for re-participation in responsible tourism. Results indicated that important factors for re-participation in responsible tourism were 'age', 'educational level', 'accompany', 'length of stay', and 'motivation'. The results implied that tourists' internal and external factors are important for re-participation in responsible tourism. It is expected that this study will contribute to the market expansion of responsible tourism.
This study explored general marriage migration for 180 unmarried Vietnamese immigrant women and identified differences in recognition after the choice of marriage. The methods used were frequency analysis, kai verification, and independent t verification were conducted. The findings were as follows. First, unmarried Vietnamese women showed a receptive attitude towards marriage migration with the less-educated group more likely to opt for marriage migration. Economic benefit expectations topped other factors in regards to the image of marriage migrant women and motivation. Dual national identity benefits were also indicated. Second, the perception of external and illusionary images of the spouses of marriage migrant women was low; however, the perception of good occupations and gender equality was high. A vague expectancy of marriage was also found. The perception was high that children from multi-cultural families would be global bilingual talents and adjust to school; however, the perception of social discrimination or prejudice was low. The perception of social discrimination was low concerning the perception of social integration towards marriage migrant women; however, the perception of identities, cultural differences and employment was present. By contrast, the group opting for marriage migration showed a significantly low perception of social discrimination and difficulty in employment. The suggested measures are to regulate and maintain forms of marriage type, reinforce prior training systems for Vietnamese immigrant women (and spouses), enhance multicultural recognition, and supplement multicultural policies.
The two dimensional quantitative structure-activity relationships (2D-QSARs) models concerning the binding affinity constants ($p[Od.]_{50}$) between 2-cyclohexyltetrahydropyrane and 2-cyclohexyltetrahydrofurane analogues as substrates, and bovine odorant binding protein (bOBP) as receptor were derived by multiple regression analyses method and discussed. The statistical quality of the optimized 2D-QSAR model (5) was good (r=0.907). From the model, the binding affinity constants ($p[Od.]_{50}$) were dependent upon the optimal value ($(TL)_{opt.}$=2.737) of total lipole (TL) of substrate molecules. Based on these findings, the high active compounds predicted by optimized 2D-QSAR model (5) were 2-(dimethylcyclohexyl)tetrahydropyrane molecule and their isomer molecules. The binding affinity constants regarding bOBP of the tetrahydrofuryl-2-yl family compounds were dependent upon the hydrophobicity (logP) of whole substrate molecules. In any case of porcine odorant-binding proteins (pOBP), the constants were dependent upon the hydrophobicity (${\pi}x={\log}P_X-{\log}P_H$) of substituents (R) in substrate molecules. Also, from the optimal values of hydrophobic constant, the hydrophobicity for bOBP influenced ca. twice time bigger (bOBP>pOBP) than that for pOBP.
Objective: Men's sexual health plays an important role in male fertility and childbearing, as it is associated with factors such as sexual desire, healthy spermatogenesis, and erectile function. In various cultures, medicinal plants have been utilized to address male sexual issues, including infertility and erectile dysfunction. Despite recent advancements in medical science for treating male impotence, some men opt for herbal supplements as an alternative, given that numerous herbs have the potential to enhance male sexual performance. The Apiaceae family is one of the oldest plant families used for medicinal purposes. Ferula, a genus within this family, comprises approximately 170 different species worldwide. Members of this genus possess numerous therapeutic properties due to the presence of various compounds. This article aims to explore the potential impacts of Ferula plants on the male reproductive system. Methods: This review article was prepared by searching for terms including Ferula and "aphrodisiac," Ferula and "spermatogenesis," and Ferula and "male reproductive system." Relevant information was gathered through electronic databases, including ISI Web of Knowledge, PubMed, and Google Scholar. Results: The findings indicated that relatively comprehensive studies have been conducted in this area, revealing that certain Ferula species have been employed in folk medicine to boost fertility and libido. Recent research has corroborated these effects. Conclusion: It is hoped that new aphrodisiac compounds with fewer side effects can be isolated from Ferula plants in the future.
Kim, Mo-Im;Harper, Paul A.;Rider, Rowland V.;Yang, Jae-Mo
Clinical and Experimental Reproductive Medicine
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v.2
no.2
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pp.13-26
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1975
Seven aspects of attitude toward marriage in Korea are examined to better understand present and future marriage patterns. Also, various facets of current marriage practice are compared with attitudes. The study comprises three groups of roughly 600 women each, selected by random sampling from a rural, an urban, and a semi-urban area. A carefully designed and pretested questionnaire was checked for reliability by a reinterview in a 15% subsample. The great majority of Korean women support traditional attitudes that one must or should marry. The small group who recommend that one should not marry are mostly the very young or the never married, whose attitudes still may change. However, there are important and probably predictive shifts in favor of more individual decision, especially among the better educated, the young, and the more urban. Traditional reasons for marriage such as "custom" and procreation are ranked first by a majority, but there is a large shift to more contemporary or liberal desire for companionship and love, also primarily among the better educated, the urban, the young, and the never married. The traditional attitude that parents should have the sole or major role in mate selection is still held by a bare majority; the educated, urban, young, and never married are more liberal. Only 6% opt for each of the two extremes: That the parent alone or the respondent alone should decide. The remainder prefer one of the two middle-of-the-road positions where parent and child together decide. The proportions of respondents who classed specified criteria as moat important for selecting a husband, arranging the criteria in order from traditional to contemporary were: Lineage, etc., 23%; personal attributes, 40%; health and education, 27%; and love, 10%. The changing attitudes are suggested by the fact that love was ranked first by only 3% of the poorly educated rural poulation versus 23% of urban college level and 31% of the urban never married. There has been a substantial rise in the ideal age of marriage over the past twelve or more years, but there also is evidence that the ideal age is at or near a ceiling. Knowledge about legal age of marriage is minimal; the implications of this for proposed legislation are discussed. Three-fifthes to four-fifths of all respondents married husbands of the same religious, residential, and economic backgrounds as themselves. Almost all of them married men of the same or higher educational level. These evidences of traditional influences in mate selection are contrasted with the low priority given some of those items in earlier questions on reasons for marriage and criterion for selecting husband. Contrary to the expressed attitudes as to who should select the husband, we find that marriages of the study sample were stated to be arranged by parents alone in 62%; and in another 23%, the parents made the decision but asked the respondent's views. Such arrangements were most frequent among the rural, the less educated, and the older respondents and less common in the urban and more educated. The implications of these and related findings are discussed.
Journal of the Korean Regional Science Association
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v.35
no.2
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pp.59-71
/
2019
Korea's average annual working hours are among the highest in the OECD. Such long-term work has been a factor that reduces the quality of life by discouraging workers' productivity and interrupting the compatibility of work and family, prompting the government to encourage flexible work systems, such as increasing part-time jobs, but a lack of quality part-time jobs. Part-time work enables flexible labor for workers, but at the same time, workers will involuntarily opt for part-time work as they have poor working conditions and negative social views. In this respect, the effect of the working type on an individual's life is expected to be different. In addition, for women, gender gaps exist in the labor market and the impact of part-time work on life satisfaction is expected to differ from men in terms of working and family alike. Using the data from the 2017 "Seoul Survey Urban Policy Indicator Survey", the ordered logistic regression model was used to analyze the cross-effect of working type and sex on satisfaction. The analysis of the study showed that when other factors were controlled, life satisfaction was high in the order of fulltime female, full-time male, part-time female, and part-time male. In addition, further analysis shows that the parttime female workers have the highest probability of choosing low life satisfaction, while the probability of choosing high life satisfaction is the lowest, and full-time male workers have the lowest probability of choosing low life satisfaction, while the highest probability of choosing high life satisfaction is the highest.
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