• Journal of Oral Medicine and Pain
• /
• v.32 no.1
• /
• pp.35-43
• /
• 2007

This study was conducted to investigate the preventing effects of OPB (water extracts of Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max) on bone loss in ovariectomized rats. Twenty Sprague Dawley rats of 13 week-old were divided into two groups: control group (ovariectomized, OVX)) and experimental group (OVX + OPB). The preventing effects of OPB on bone loss, OPB were fed with 100 mg OPB/kg body weight from 3 days after ovariectomization. The duration of the treatment period was 8 weeks. All bone mineral density, bone mineral content indices and bone strength indices measured by peripheral quantitative computerized tomography (pQCT) and serum bone marker assessment were carried out at end of experiment. pQCT scanning showed that OVX induced a significant decrease in cancellous bone mineral density in the proximal tibia ($-29.8{\pm}3.0%$). These decreases were significantly prevented by the administration of OPB 100 mg/kg ($-21.4{\pm}2.3%$. p<0.05). Bone strength indices showed significant difference between OVX and OPB treated rats (anti-fracture, anti-twisting, p<0.05). These data suggested that administration of OPB inhibited the loss of bone in OVX rats. CTx level were lower than in the OPB-treated animals compared with OVX. However there was no significant difference between OVX and OPB treated OVX rat. Our results suggest that OPB is effective in preventing the development of bone loss induced by ovariectomy in rats.

• International Journal of Oral Biology
• /
• v.32 no.4
• /
• pp.143-151
• /
• 2007

This study was conducted to investigate the preventing effects of OPB (Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max extracts) and combined OPB/Calcium therapy on bone loss in ovariectomized rats. Sixty Sprague Dawley rats of 12-week-old were divided into eight groups: OVX (ovariectomized), OPBL (OPB 50 mg/kg), OPBM (OPB100 mg/kg), OPBH (OPB 200 mg/kg), OPBL/CAL(OPBL+CAL), OPBM/CAL (OPBM+CAL), OPBH/CAL (OPBH+CAL) and CAL (Calcium citrate 88.33 mg/kg+1$\alpha$, 25-dihydroxy-vitamin $D_3$ 33.33 IU/kg). Bone mineral density (BMD), bone mineral content (BMC), bone strength indices and cortical thickness were analyzed by peripheral quantitative computerized tomography (pQCT). pQCT scanning showed that OVX induced a significant decrease in trabecular bone mineral density and bone mineral content in the proximal tibia $(-36.4\pm2.4%,\;-21.8\pm12.7%)$. These decreases were significantly prevented by the administration of OPBM and OPBM/CAL. Cortical BMD and BMC of tibia were slightly enhanced by OPB and OPB/CAL. However there was no significant difference between OVX and OPB, OPB/CAL treated group. Bone strength indices and cortical thickness were not significantly different. Our results suggest that OPB and combined OPB/Calcium therapy are effective in preventing the development of bone loss induced by ovariectomy in rats.

• Journal of the Society of Naval Architects of Korea
• /
• v.53 no.3
• /
• pp.237-248
• /
• 2016

As OPB and IPB moment-induced fatigue damage on mooring chain links were reported for a offloading buoy, verification of OPB and IPB fatigue has been a key engineering item in offshore structure mooring design. Mathematical and physical features of the conventional approach which was mainly explained in BV guideline are reviewed and disadvantages of the conventional approach are addressed in terms of stress proportionality and nonlinearity of OPB and IPB moments. In order to eradicate these disadvantages, a novel approach is newly proposed which is able to dispel apprehension on stress proportionality and is not dependent of nonlinearities of OPB and IPB moments. Significant differences between two approaches are suggested by comparing relations of OPB moment versus OPB interlink angle and IPB moment versus IPB interlink angle. For periodic OPB tension angle processes having three different OPB angle ranges with a simple irregular tension process, fatigue damage calculation reveals that OPB moment-induced fatigue damage has dominant portion to total fatigue damage. Comparative studies between two approaches also show that the conventional approach based on BV guideline predicts fatigue damage far conservatively since it assume unrealistic high stress concentration factor for tension load. Meanwhile IPB moment-induced fatigue damage is negligible compared to tension-induced fatigue damage.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.5
• /
• pp.309-313
• /
• 1998

RAPD (Random Amplified Polymorphic DNA) analysis was conducted to Schisandra nigra plants in order to select the specific markers for monoecious and dioecious individuals. RAPD results using eighty random 10-mer primers revealed that S. nigra had a different banding pattern from S. chinensis and Kadsura japonica. When DNA isolated from leaves of monoecious and dioecious plants were used as PCR template, only five primers, OPA-17, OPA-19, OPB-03, OPB-09 and OFB-16, showed polymorphic band patterns. No variation in banding profiles within male or female individuals was observed when these five primers were used whereas three monoecious plants (No 1, No 2 and No 3) showed different banding patterns one another, A 750 bp segment was amplified by primer OPB-3 from male individuals. On the other hand, two segments, 950 bp and 1690 bp, with OPA-19 and 700 bp of segment with OPB-3 were amplified in female individuals. These result indicate that the specific buds of male and female S. nigra could be used as genetic markers for the early discrimination of male and female individuals.

• Journal of the Society of Naval Architects of Korea
• /
• v.47 no.4
• /
• pp.580-588
• /
• 2010

OPB(out-of-plane bending)-induced failure of mooring chain was firstly addressed by CALM (catenary anchor leg mooring)-type offloading buoy, located approximately one mile away from the bow of the Girassol FPSO which was installed offshore area of Angola in September 2001. This study deals with verifying the load transfer mechanism between the first free chain link and connected two chain links inside the chain hawse. OPB moment to angle variation relationships are proposed by extensive parametric study where the used design variables are static friction coefficients, proof test loads, nominal tension forces, chain link diameters, chain link grades and chain link types. The stress ranges due to OPB moments are obtained using nonlinear FEAs (finite element analyses). Final stress ranges are derived considering ones from IPT (in-plane tension) forces. Also a formula for OPB fatigue assessment is briefly introduced.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.12
• /
• pp.1655-1658
• /
• 2001

Bovine genome samples were collected from meat of three different beef breeds (Hanwoo, Holstein and imported beef breed) that are commercially merchandized in Korean beef market. Operon B (OPB)-kits were used as random primers (3, 7, 10, 11, 12, 14) in random amplified polymorphic DNA (RAPD) method on whole genome. Each primer provided characteristic bands that were highly polymorphic. Each single primer could provide relatively efficient polymorphic band patterns among breeds. However, use of two or more primers in combination is recommended to improve resolution of experiments with higher molecular weight bands of DNA. In our experiments, OPB-11 resolved well between beef cattle breeds and Holstein. And OPB-7, 12 and 14 could be combined with OPB-11 to identify Hanwoo beef from the other two kinds of beef.

• The Journal of Korean Academy of Prosthodontics
• /
• v.46 no.1
• /
• pp.31-41
• /
• 2008

Statement of problem: It has been proved that Pleurotus eryngii Quel and Eleutherococcus senticosus have antiinflammatory action and not only stimulates the proliferation and activity of osteoblast but inhibits the generation and activity of osteoclast in vitro. Pleurotus eryngii Quel and Eleutherococcus senticosus are the main component of $OPB-K^{(R)}$. Purpose: The purpose of this study was to evaluate $OPB-K^{(R)}$ which enhances the healing rate of peri-implant bone and the bone mineral density. Materials and methods: Thirty six specially designed implants were installed in the tibia of rats. The group medicated with $OPB-K^{(R)}$ was the experimental group, and that without was the control group. hen the implant stability was measured by $Periotest^{(R)}$. Bone mineral density and histological measurement were conducted at the 2nd, 4th and 6th week $Periotest^{(R)}$ and bone mineral density values were analyzed statistically with independent t-test at 95% confidence level(p<0.05). Results: The results of this study were as follows : 1. There was no statistically significant difference in $Periotest^{(R)}$. values between the experimental group and control group at the 2nd week, however, on the 4th and 6th week there was significant difference(p<0.05). 2. There was no statistically significant difference in bone mineral density between the experimental group and control group at the 2nd and 4th week, however on the 6th week there was significant difference(p<0.05). 3. Histological analysis showed difference in osseointegration on the 4th and 6th week between the groups. Conclusion: From the results, it is concluded that the $OPB-K^{(R)}$ medicated group showed statistically better results in bone density and stability than the control group. Clinically it would be better to medicate $OPB-K^{(R)}$ to patients for a long period of time after implantation to get superior results.

• Journal of Ocean Engineering and Technology
• /
• v.31 no.4
• /
• pp.299-307
• /
• 2017

The design of the mooring line to maintain the position of an offshore structure in rough marine environments is recognized as a very important consideration. Conventional fatigue evaluation of a mooring line was performed by considering the tensile force acting on the mooring line, but the mooring line broke after 238 days in the girassol area even though the expected fatigue life was expected to be longer. The causes of this event are known to be due to OPB/IPB (out-of-plane bending/in-plane bending) caused by chain link friction due to the excessive tensile strength of the mooring line. In this study, three models with different boundary conditions were proposed for fatigue analysis of a mooring line considering OPB/IPB. Interlink stiffness was calculated by nonlinear structure analysis and a stress concentration factor was derived. In addition, the sensitivity of interlink stiffness according to the magnitude of tensile force, large deformation effect, and coefficient of friction was analyzed, and the effect of critical elastic slip and bending moment calculation position on interlink stiffness was confirmed.

• International Journal of Naval Architecture and Ocean Engineering
• /
• v.11 no.1
• /
• pp.178-201
• /
• 2019

The appropriate design of a mooring system to maintain the position of an offshore structure in deep sea under various environmental loads is important. Fatigue design of the mooring line considering OPB/IPB(out-of-plane bending/in-plane bending) became an essential factor after the incident of premature fatigue failure of the mooring chain due to OPB/IPB in the Girassol region in West Africa. In this study, mooring line fatigue analysis was performed considering the OPB/IPB of a spread moored FPSO in deep sea. The tension of the mooring line was derived by hydrodynamic analysis using the de-coupled analysis method. The floater motion time histories were calculated under the assumption that the mooring line behaves in quasi-static manner. Additional time domain analysis was carried out by prescribing the obtained motions on top of the selected critical mooring line, which was determined based on spectral fatigue analysis. In addition, nonlinear finite element analysis was performed considering the material nonlinearities, and both the interlink stiffness and stress concentration factors were derived. The fatigue damage to the chain surface was estimated by combining both the hydrodynamic and stress analysis results.

• Journal of Aquaculture
• /
• v.19 no.2
• /
• pp.67-76
• /
• 2006

Genomic DNA isolated from two Porphyra species, P. tenera and P. dentate from Wando located on the southern coast of Korean peninsula was amplified by PCR reaction. The amplified products were separated by agarose gel electrophoresis (AGE) with decamer primer and stained with ethidium bromide. The eight arbitrarily selected primers OPA-04, OPA-06, OPB-01, OPB-08, OPB-10, OPB-11, OPB-14 and OPC-10 generated the shared loci, polymorphic, and specific loci. The size of DNA bands varies from 100 bp to 2,200 bp. The complexity of the banding patterns varies dramatically between the primers and two Porphyra species. A total of 528 loci observed were identified in P. tenera and 443 in P. dentata: 22 polymorphic loci (4.2%) in P. tenera and 30 (6.8%) in P. dentata. 154 shared loci observed, the average 19.3 per primer, were identified in P. tenera and 143 loci, the aver-age 17.9 per primer, in P. dentata species. The number of specific loci in P. tenera and P. dentata was 73 and 77, respectively. The average bandsharing value was $0.623{\pm}0.008$ with P. tenera and $0.560{\pm}0.009$ within P. dentata. The average bandsharing value between two Porphyra species was $0.408{\pm}0.004$, ranged from 0.305 to 0.564. The dendrogram obtained by the eight primers indicates four genetic clusters. The genetic distance between two Porphyra species ranged from 0.076 to 0.627. The individual no. 02 of P. tenera was genetically closely related to no. 01 of P. tenera(genetic distance=0.082). Especially, two entities between the individual DENTATA no.21 and DENTATA no. 19 of P. dentata showed the longest genetic distance (0.627) in comparison with other individuals used. In this study, RAPD-PCR analysis has revealed the significant genetic distance between two Porphyra species pairs (P<0.001).