Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Application of RAPD Methods in Meat for Beef Breed Identification

  • Choy, Y.H. (Department of Animal Science and Resources, Dankook University) ;
  • Oh, S.J. (Department of Animal Science and Resources, Dankook University) ;
  • Kang, J.O. (Department of Animal Science and Resources, Dankook University)
  • Received : 2001.03.04
  • Accepted : 2001.07.16
  • Published : 2001.12.01
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Abstract

Bovine genome samples were collected from meat of three different beef breeds (Hanwoo, Holstein and imported beef breed) that are commercially merchandized in Korean beef market. Operon B (OPB)-kits were used as random primers (3, 7, 10, 11, 12, 14) in random amplified polymorphic DNA (RAPD) method on whole genome. Each primer provided characteristic bands that were highly polymorphic. Each single primer could provide relatively efficient polymorphic band patterns among breeds. However, use of two or more primers in combination is recommended to improve resolution of experiments with higher molecular weight bands of DNA. In our experiments, OPB-11 resolved well between beef cattle breeds and Holstein. And OPB-7, 12 and 14 could be combined with OPB-11 to identify Hanwoo beef from the other two kinds of beef.

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Cited by

  1. Detection of DNA Fragment to Differentiate Korean Cattle vol.15, pp.8, 2001, https://doi.org/10.5713/ajas.2002.1071
  2. Genomic approaches to economic trait loci and tissue expression profiling: application to muscle biochemistry and beef quality vol.66, pp.1, 2004, https://doi.org/10.1016/s0309-1740(03)00020-2