• 한국식품과학회지
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• 제36권1호
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• pp.147-151
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• 2004

난백에 NaOH, 열, 및 효소를 처리하였을 때, 난백 중의 ovomucoid(OM) 및 ovalbumin(OA)의 항원성 변화를 항OM 및 항OA 항체를 이용하여 간접경합 ELISA로 조사하였다. 효소처리는 OM의 항원성을 효과적으로 감소시키지 못한 반면 OA의 항원성은 식물성, 미생물성 protease를 사용하였을 때 약 1/5,000-1/100,000로 감소되었다. 열처리를 한 난백 중 OM은 $100^{\circ}C$까지 거의 변화하지 않으나 $121^{\circ}C$ 처리에서는 난백의 항원성이 OM은 1/250으로 감소되었다. OA의 항원성은 열처리에 의해 오히려 증가되었으며 최고 100배까지 증가되는 것으로 나타났다. NaOH를 농도별로 처리한 난백 중 OM의 항원성 변화는 0.3%부터 감소하기 시작하여 1%이상에서 항원성이 거의 제거되었다. 반면 OA의 경우는 모든 NaOH 처리농도에서 항원성이 증가하였다. NaOH 처리에 추가적인 열처리($70^{\circ}C$, 15분)를 실시한 것은 난백중 OM과 OA의 항원성 감소에 다소 효과적인 것으로 나타났다.

• 한국식품영양과학회지
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• 제37권11호
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• pp.1389-1394
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• 2008

본 연구는 산겨릅나무의 식품학적 가치를 알아보고 기능성식품 소재로서의 이용.개발을 위하여 항산화 및 간 기능 보호효과를 탐색하였다. 항산화성 결과 가운데 총 폴리페놀은 EtOAc fr.에서 함량이 가장 많았고 BuOH fr., EtOH ex., $CHCl_3$ fr., hot water ex., aqueous fr. 순으로 높게 나타났다. 전자공여능은 EtOAc fr.과 BuOH fr.이 ascorbic acid와 유사한 전자공여능을 보였으며 SOD 유사활성능은 $250\;{\mu}g/mL$ 이하의 농도에서는 BuOH fr.이 가장 높게, 그 이상에서는 aqueous fr.에서 가장 높게 나타났다. LPS로 급성 간 손상을 유도한 마우스에서의 BuOH fr.의 간 기능 보호효과에서는 BuOH fr.을 전 처치한 다음 LPS를 투여한 군이 LPS만을 투여한 군보다 혈장 AST와 ALT의 활성은 유의하게 감소 되었으나 BUN의 농도는 유의성이 관찰되지 않았다. 간조직의 형태학적 관찰에서 BuOH fr.을 투여하고 LPS로 급성 간 손상을 유도한 군의 간세포는 염증과 괴사가 나타났지만 LPS군에 비하여 그 정도가 적은 것으로 나타났다.

• 한국의류학회지
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• 제11권2호
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• pp.101-112
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• 1987

The purpose of this study is to investigate the influence of addition of sodium dodecyl sulfate(SDS) when wool is treated with sodium hydroxide(NaOH). Physical and chemical changes were examined on wool treated with various cone. of NaOH and SDS simultaneously. The result are as follows. 1. The higher the temperature and the longer the time of NaOH treatment are, the more alkali damage wool get : increase in weight loss and decrease in urea-bisalphite solubility and in tensile strength. But the treatment time reacts less effective than the temperature. 2. When wool is treated with NaOH, at NaOH cone. under $10^{-3}M$., the addition of SDS alleviates the alkali reaction on wool: increase in cystine contents and in urea-bisulpite solubility, and decrease in degree of swelling. On the other hand, at NaOH cone. over $10^{-2}M$., the addition of SDS promotes the alkali reaction on the wool. 3. When wool is treated with NaOH, the addition of SDS shows no changes on the tensile strength. On the other hand, at NaOH cone. under $10^{-2}M$., the addition of SDS shows no changes on the softness of wool, but at the $10^{-1}M$. NaOH cone. addition of SDS increases the soft-ness of wool. The area shrinkage of wool treated with NaOH and SDS shows less changes than with NaOH only.

• 한국염색가공학회지
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• 제13권2호
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• pp.120-127
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• 2001

In order to make a microfiber fabric with PET/Co-PET Sea-Island Type microfiber, the optimum condition of extraction and elimination of Co-PET from the mocrofiber was examined. At the same time, the physical property change of the fabric with respect to the change of the relative amount of the Co-PET in the microfiber was also examined to provide a directly applicable data set to the industry. The sample fabric used was warp 75/36(DTY) and weft 0.05d(PET/Co-PET, Sea Island Type Microfiber) twill fabric of 36 separated yarns＋40/24(high shrinking yarn) with 130/48 ITY. The data set was made at various NaOH concentrations and steam temperatures with time as a main variable. The physical properties examined were the tensile properties. The results obtained were the tensile. The results obtained were 1. For a proper extraction of Co-PET (13.5%)from the microfiber with wet curing, it takes more than 5 min. in 8 and 12% of NaOH solutions but it takes only 3 min. in 18% of NaOH solution at 12$0^{\circ}C$. 2. For a proper extraction of Co-PET (13.5%) from the microfiber with wet curing, ti takes 3~5min. in 12 and 14% of NaOH solution and it takes less than 3 min. in 18% of NaOH solution at $130^\circ{C}$. 3. The increasing ratio of WT increased with increasing NaOH concentrations and the equilibrium point reached was 3 min. at $120^\circ{C}$. 4. The WT increasing ratio was greater in 14 and 18% NaOH solutions than in 8 and 12% of NaOH solutions at $130^\circ{C}$5. The RT ratio changes at $120^\circ{C}$ in 8 and 12% of NaOH solutions were indifferent from that at $130^\circ{C}$ in 12% of NaOH solution. However, the RT was apparently decreased with increasing NaOH concentration.

• 한국염색가공학회지
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• 제13권2호
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• pp.34-34
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• 2001

In order to make a microfiber fabric with PET/Co-PET Sea-Island Type microfiber, the optimum condition of extraction and elimination of Co-PET from the microfiber was examined. At the same time, the physical property change of the fabric with respect to the change of the relative amount of the Co-PET in the microfiber was also examined to provide a directly applicable data set to the industry. The sample fabric used was warp 75/36(DTY) and weft 0.05d(PET/Co-PET, Sea Island Type Microfiber) twill fabric of 36 separated yarns＋40/24(high shrinking yarn) with 130/48 ITY. The data set was made at various NaOH concentrations and steam temperatures with time as a main variable. The physical properties examined were the tensile properties. The results obtained were the tensile properties. The results obtained were 1. For a proper extraction of Co-PET (13.5%)from the microfiber with wet curing, it takes more than 5 min. in 8 and 12% of NaOH solutions but it takes only 3 min. in 18% of NaOH solution at 120℃. 2. For a proper extraction of Co-PET (13.5%) from the microfiber with wet curing, it takes 3∼5 min. in 12 and 14% of NaOH solution and it takes less than 3 min. in 18% of NaOH solution at 130℃. 3. The increasing ratio of WT increased with increasing NaOH concentrations and the equilibrium point reached was 3 min. at 120℃. 4. The WT increasing ratio was greater in 14 and 18% NaOH solutions than in 8 and 12% of NaOH solutions at 130℃. 5. The RT ratio changes at 120℃ in 8 and 12% of NaOH solutions were indifferent from that at 130℃ in 12% of NaOH solution. However, the RT was apparently decreased with increasing NaOH concentration.

• 생명과학회지
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• 제24권1호
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• pp.74-80
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• 2014

본 연구에서는 다양한 약효가 있다고 알려진 애기땅빈대(Euphorbia supina)의 추출물 및 분획물의 지방산 조성을 분석하고 인체 암세포 증식 억제효과에 대하여 살펴보았다. A+M과 MeOH 추출물의 지방산 조성 패턴은 유사했고 A+M과 MeOH 추출물은 각각 53.4% 및 42.1%의 18:3n-3를 함유하였다. 분획물들 중 85% aq. MeOH는 가장 높은 함량의 18:3n-3를 나타내었다. 애기땅빈대에 의한 암세포 증식 억제효과는 MeOH 추출물과 비교했을 때 A+M 추출물에 의한 억제효과가 높았으며 AGS 인체 위암세포에 대한 억제효과가 높았다. 분획물들 중에서는 n-Hexane과 85% aq. MeOH 분획물들에 의한 증식 억제효과가 높았다. 따라서 애기땅빈대의 항암 활성 성분은 n-Hexane과 85% aq. MeOH 분획물들에 함유되어 있는 것으로 여겨지며 향후 정제하여 규명할 필요가 있다고 사료된다.

• 한국식품영양과학회지
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• 제27권1호
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• pp.141-148
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• 1998

This study describes the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3, calcitriol] analog, 1,25(OH)2-16ene-23yne-D3 on proliferatin and differentiatin of the human histiocytic lymphoma cell line U937. This paper also describes the effects of 1,25(OH)2-16ene-23yne-D3 on ${\gamma}$-interferon(IFN-${\gamma}$) synthesis by phytohemagglutinin-activated peripheral blood lymphocytes(PBLs). In the present investigation, 1,25(OH2)-16ene-23yne-D3 was compared to the natural metablite of vitamin D3, 1,25(OH)2D3. 1,25(OH)2-16ene-23yne-D3 was more potent than 1,25(OH)2D3 for inhibition of proliferation and induction of differentiation of U937 cells, Its effects on inhibition of proliferation was about 30-fold more potent than 1,25(OH)2D3. On induction of differentiation as measured by nonspecific esterase (NSE) activity and morphologic change, this analog morphologically and functionally differentiated U937 cells to monocyte-macrophage phenotype showing a decrease of N/C ration in Giemsa staining and the increase of adherence ability of surface. After 3 days in culture, a more significant supression of IFN-${\gamma}$ synthesis analog on supression of IFN-${\gamma}$ synthesis was a dose-dependent manner, with peak activity at 10-7M. The strong direct effects of 1,25(OH)2-16ene-23yne-D3 on cell proliferation and cell differentiation, make this compound an interesting candidate for clinical studies for several types of malignancies, and the effects on supression of IFN-${\gamma}$ synthesis provide the further evidence for a role of 1,25(OH)2-16ene-23yne-D3 in immunoregulation.

• 자원리싸이클링
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• 제5권3호
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• pp.50-55
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• 1996

CaO를 $HNO_3$로 용해하여 제조한 (NO$Ca_3$)$_2$용액을 $NH_4$OH로 pH를 조절하고, 상압하의 온도에서 (NH$_4$)$_2$HPO$_4$를 주입시켜서 소정의 시간동안 Ca,,(PO$_4$),(OH)$_2$합성실험을 하였다. Ca,,(PO$_4$),(OH)$_2$는 pH 10~13 범위에서 생성되었고 이 HAP의 입경을 전자현미경으로 조사한 결과 0.1~0.5$mu extrm{m}$이었다. 반응온도는 $40~70^{\circ}C$, 결정화온도 $90^{\circ}C$이며 반응시간은 30분이 적당하였다. 생성된 Ca\ulcorner(PO$_4$)\ulcorner(OH)$_2$는 $500^{\circ}C$, 1시간동안 하소하여도 변화하지 않으나 80$0^{\circ}C$에서 1시간동안 하소한 시료는 $Ca_3$($PO_4$)$_2$와 HAP, CaO로 변화되었고, 구형의 미립자로 되어 있었다.

• 한국식품위생안전성학회지
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• 제17권3호
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• pp.124-128
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• 2002

본 연구에서는 알코올 섭취 방법이 간과 뇌에서 산화적 스트레스 유발 정도에 미치는 영향을 알아보고자 횐쥐에 5%의 에탄올이 함유된 물을 1개월간 자유로이 섭취하게 하는 방법 또는 2.5g/kg의 에탄올을 1일 1회 1개월간 경구 투여하는 방법 등 알코올 투여 방법을 달리하고 간, 대뇌 및 소뇌에서 지질과산화 반응과 비타민 E의 함량 변화를 비교하였다. 5%-EtOH군의 간조직에서는 비타민 E의 함량이 증가하고 지질과산화물은 변화가 없었으며, 2.5g-EtOH군의 간조직에서는 비타민 E의 함량은 변화가 없으나 지질과산화물은 유의적으로 증가하였다. 뇌 조직에서는, 5%-EtOH군의 대뇌와 소뇌 모두에서 지질과산화물이 증가하고 비타민 E의 함량이 감소하였다. 또한, 2.5g-EtOH군의 대뇌와 소뇌에서도 지질과산화물이 증가하고 비타민 E의 함량이 감소하였으며 그 정도는, 5%-EtOH군에서 보다 큰 경향을 보였다. 결론적으로, 간에서의 지질과산화 반응 및 비타민 E의 함량은 알코올 투여 방법에 따라 다양하게 나타났으나 대뇌와 소뇌에서는 본 연구에서 실시한 투여 방법 모두에 의해 산화적 스트레스가 유발되었다.

• 한국식품영양과학회지
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• 제24권1호
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• pp.1-9
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• 1995

1, 25(OH)2-23ene-D3 is a novel vitamine D3 analog which has a double bond between C-23 and C-24. We describe the effects of this analog on cell differentiation and cell proliferation in vitro using the human histiocytic lymphoma cell line U937, and on calcium metabolism in rats in vivo. In the present investigation 1, 25(OH)2-23ene-D3 was compared to the natural metabolite of vitamin D3, 1$\alpha$, 25-dihydroxycholecalciferol[1, 25(OH)2-23ene-D3 was more potent than 1, 25(OH)2-23ene-D3 for inhibition of proliferation and induction of differentiation of U937 cells. Especially, its effect on induction of differentiation, as measured by superoxide production and nonspecific esterase(NSE) activity, was about 20-fold more potent that 1, 25(OH)2-23ene-D3. This analog morphologically and functionally differentiated U937 cells to monocyte-macrophage phenotype showing a decrease of N/C ratio in Giemsa staining and the increase of adherence ability to surface. Intraperitoneal administration of 1, 25(OH)2-23ene-D3 to rats showed that the compound had at least 50 times less activity than 1, 25(OH)2-23ene-D3 in causing hypercalcemia and hypercalciuria. The strong direct effects of 1, 25(OH)2-23ene-D3 on cell proliferation and cell differentiation, coupled with its decreased activity of calcium metabolism make this compound an interesting candidate for clinical studies including patients with leukemia, as well as several skin disorders, such as psoriasis.