• 제목/요약/키워드: OCT4B

검색결과 81건 처리시간 0.027초

가두리 양식장 주변의 수중환경소음과 생물소음의 특성에 관한 연구 (A Study on the Characteristics of the Underwater Ambient Noise and Biological Noise in Fish Farm Cages)

  • 박태건
    • 수산해양기술연구
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    • 제35권1호
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    • pp.41-49
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    • 1999
  • This paper describes to analyze the underwater ambient noise and biological noise of cultivating fishes in the fish farm cages at the seawater Tongyong-kun, KyongNam and lake of Chungju, Chech'on, ChungBuk from 10 to 19 Oct. 1997, in order to find out the characteristics of these noises. The results obtained were as follows; (1) The ambient noise around the fish farm cages at lake of Chungju was 10~200Hz frequency range, 70~105dB spectrum level. The central frequency was 50~70Hz, changing of ambient noise was getting bigger than 10~200Hz in 200Hz~2kKz frequency by wind, water current. (2) The frequency of noise source around the fish farm cage at the seawater of Tongyong-kun was 20~200Hz, spectrum level was 80~100dB while feed factory was working around the fish farm cage. When feed factory did not work, noise source was 10~600Hz frequency range, 70~90dB spectrum level. It was 10dB less than that of while feed factory was working, and then the central frequency was 70Hz. (3) The vessel noise of excursion ship had changed largely at 100dB spectrum level in 10~500Hz frequency band, and the fishing boat had 20Hz~2kHz frequency range. (4) The biological noise in the fish farm cage at lake of Chungju, which was feeding of Cyprinus carpio, 2was 10~30Hz frequency, 70~104dB spectrum level. The central frequency was 75Hz. The biological noises in the fish farm cage at the seawater of Tongyong-kun, which were feeding and swimming noise, had very different spectrum pattern by species, and the frequency band was 10~800Hz.

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Establishment of a Simple and Effective Method for Isolating Male Germline Stem Cells (GSCs) from Testicular Cells of Neonatal and Adult Mice

  • Kim Kye-Seong;Lim Jung-Jin;Yang Yun-Hee;Kim Soo-Kyoung;Yoon Tae-Ki;Cha Kwang-Yul;Lee Dong-Ryul
    • Journal of Microbiology and Biotechnology
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    • 제16권9호
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    • pp.1347-1354
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    • 2006
  • The aims of this study were to establish a simple and effective method for isolating male germline stem cells (GSCs), and to test the possibility of using these cells as a new approach for male infertility treatment. Testes obtained from neonatal and adult mice were manually decapsulated. GSCs were collected from seminiferous tubules by a two-step enzyme digestion method and plated on gelatin-coated dishes. Over 5-7 days of culture, GSCs obtained from neonates and adults gave rise to large multicellular colonies that were subsequently grown for 10 passages. During in vitro proliferation, oct-4 and two immunological markers (Integrin ${\beta}1,\;{\alpha}6$) for GSCs were highly expressed in the cell colonies. During another culture period of 6 weeks to differentiate to later stage germ cells, the expression of oct-4 mRNA decreased in GSCs and Sertoli cells encapsulated with calcium alginate, but the expression of c-kit and testis-specific histone protein 2B(TH2B) mRNA as well as the localization of c-kit protein was increased. Expression of transition protein (TP-l) and localization of peanut agglutinin were not seen until 3 weeks after culturing, and appeared by 6 weeks of culture. The putative spermatids derived from GSCs supported embryonic development up to the blastocyst stage with normal chromosomal ploidy after chemical activation. Thus, GSCs isolated from neonatal and adult mouse testes were able to be maintained and proliferated in our simple culture conditions. These GSCs have the potential to differentiate into haploid germ cells during another long-term culture.

협동학습이 일부 초등학생의 성교육에 미치는 효과 (Effects of Cooperative Learning Methods on Sex Education among Primary School Students)

  • 류정은;김윤신;김현숙
    • 한국학교보건학회지
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    • 제25권1호
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    • pp.122-132
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    • 2012
  • Purpose: The purpose of this study is to determine the effectiveness of cooperative learner-centered methods of sex education. Methods: This study was carried out on 5th grade elementary school students in D-district. Nine classes were divided into 3 groups using each different teaching methods: group A (a cooperative learning), group B (a lecture) and group C (a control group for a comparison). The study period was from Oct. 17 to Dec. 2 in 2011. Both groups A and B received sex education lessons for 40 minutes for 4 weeks and were tested their sex knowledge and attitude to compare the differences. Results: The scores of sex knowledge for all three groups were increased and their sex attitude was increased as well. The points of sex knowledge between pre and post test in group A are greater than the group B's. Thus, the cooperative learning approach with Group A was more effective to improve student's sex knowledge. But the difference between the sex attitude scores was not statistically significant. Group A and B showed a positive improvement in both their sex knowledge and attitudes compared with the control group. Conclusion: This experiment shows that an active teaching methods is more effective to improve student sex knowledge than a passive approach. Thus, a cooperative learning method results in increases of both student's sex knowledge and interests in learning sex education. It needs to develop more diverse teaching methods and programs on sex education that are more systematic and tailored.

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길항미생물에 의한 Rhizoctonia spp.의 억제 및 길항미생물의 농약 혼용시 생존율 (Suppression of Rhizoctonia spp. by Antagonistic Microorganisms and Their Compatibility with Fungicides)

  • 이상재;심경구;김영권;허근영
    • 아시안잔디학회지
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    • 제12권1호
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    • pp.23-30
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    • 1998
  • 174 isolates of soil microorganisms were isolated from E-golf club from Apr.1997 through Oct. 1997. And 27 strains of them were selected through the inhihition test of mycelial growth. In the same period, soil-borne diesease pathogens, "Rhizoctonia", causing Large patch, Brown patch, Spring dead spot, and Yellow patch were isolated from the diseased areas in E-golf and S-golf club. The antagonistic activity of the strains against the pathogens was tested to select the excel-lent antagonists. In contact with the fungicides, the survivability of the antagonists was tested to assess the compatibility of the antagonists with the pesticides. The results were as follows: 1.Suppression of Rhizoctonia by Antagonists. Antagonistic activity of 27 strains against the pathogens was: tested in vitro. In the result, 3 isolates(B-7, B-15, B-41) of bacteria and 2 isolates(F-5, F-47) of fungi were superior to the rest. 2.Compatibility of the antagonists: with the fungicides: With 13 kinds of pesticides widely using Golf Club, Compatibility of 5 antagonists: were finally tested to select the strains: that mostly survived in contact with pesticides. In the results:, two of five strains: were selected : one strain was bacteria B-15, the other strain was fungi F-47. 24h after the mixing with pesticides:, these two strains were shown to survive at 90% level and these were identified as Bacillus and Trichoderma, respectively. And the most compatible pesticides: with the antagonists were shown to Polytoxin-D thirarn(s:urvivability 99.4%) and Validamycin-A (survivability 98.6%). Keywords:Antagonist, Large Patch, Trichoderma, Compatibility, Fungicide.Fungicide.

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종합병원 간호사의 조직시민행동 영향요인 (Factors Affecting the Organizational Citizenship Behaviors of General Hospital Nurses)

  • 임경민;정귀임
    • 보건의료산업학회지
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    • 제9권4호
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    • pp.51-62
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    • 2015
  • Objectives : Identify the factors that affect organizational citizenship behavior of hospital nurses, and to provide a basis for developing an effective nursing workforce management system. Methods : Participants of this study were 214 nurses serving at general hospitals located in B Metropolitan City. Data collection was performed from Oct.15th to 25th, 2014. The methods of analyzing data were ANOVA, Scheffe's-Test, Pearson's Correlation Coefficient Analysis and the Stepwise Multiple Regression Analysis according to the SPSS WIN 18.0 Program. Results : Emotional intelligence explained 42.1% of the subjects' organizational citizenship behaviors. This figure increased to 48.7, 51.3 and 52.3%, respectively when other three factors of job satisfaction, age and educational background were added on a one-by-one basis. Conclusions : Considering that emotional intelligence is a critical factor in organizational citizenship behaviors, general hospitals should develop and implement education and training programs that can raise their nurses' emotional intelligence and job satisfaction and, concomitantly, promote their organizational citizenship behaviors provided that feedbacks is provided later.

생쥐 생식줄기세포의 체외 분리 및 증식 (In Vitro Isolation and Proliferation of Mouse Male Germ-Line Stem Cells)

  • 김수경;김계성
    • 한국수정란이식학회지
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    • 제18권3호
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    • pp.243-248
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    • 2003
  • 1. 생쥐 고환으로부터 얻은 세포를 배양하여 군집을 형성하는 것을 관찰할 수 있었으며, AP, SSEA-1, -3, -4과 Integrin $\alpha$6, $\beta$1 및 Oct4의 발현을 확인하였다. 2. 생쥐 생식줄기세포를 3-5일정도 배양하게 되면, 여러 층으로 이루어진 군집을 이루게 되는데 이는 생쥐 배아줄기세포나 배아생식줄기세포의 형태와 같은 것이었다. 3. 생쥐 생식줄기세포를 체외에서 효과적으로 분리, 배양할 수 있는 조건을 확립하였다.

뇌 컴퓨터단층검사 시 양자잡음제거 알고리즘을 적용한 영상의 비교평가 (Comparative Evaluation of Images after Applying Quantum Denoising System Algorithm to Brain Computed Tomography)

  • 조평곤
    • 대한방사선기술학회지:방사선기술과학
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    • 제40권4호
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    • pp.589-594
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    • 2017
  • 본 연구의 목적은 뇌 컴퓨터단층검사 시 양자잡음제거(Quantum Denoising System; QDS) 알고리즘을 적용한 영상 분석을 통해 화질 향상 효과를 알아보고자 한다. 2017년 7월부터 2017년 10월까지 경북 소재 G 영상의학과에 뇌 컴퓨터단층검사를 위해 내원한 45명의 성인을 대상으로 동의하에 후향적 연구를 하였고, 뇌 컴퓨터단층검사 시 QDS(-)를 적용하지 않은 그룹(A Group)과 QDS(+)를 적용한 그룹(B Group)으로 나누어 검사하였다. 다음과 같은 결론을 얻었다. 노이즈값은 Pons부분과 Vermis부분 모두 QDS(+)를 적용한 B그룹에서 통계적으로 유의하게 낮았다(A Group; Pons $6.92{\pm}0.98HU$, Vermis 6.72, B Group; Pons $5.41{\pm}1.05HU$, Vermis $5.28{\pm}0.73HU$ : p<0.05). SNR값은 Pons부분과 Vermis 부분 모두 QDS(+)를 적용한 B그룹에서 통계적으로 유의하게 높았다(A Group; Pons $5.21{\pm}1.28$, Vermis $6.23{\pm}1.49$, B Group; Pons $7.28{\pm}2.56$, Vermis $8.63{\pm}3.04$ : p<0.05). 결론적으로 뇌 컴퓨터단층검사 시 양자잡음제거 알고리즘을 적용한다면 영상의 노이즈 감소 및 신호 대 잡음비(SNR), 대조도 대 잡음비(CNR)를 좀 더 개선시켜 진단에 적절한 영상을 얻을 수 있을 것으로 생각된다.

Characteristics of Environmental Solar Ultraviolet Irradiance

  • Sasaki, Masako;Oyanagi, Takehiko;Takeshita, Shu
    • Journal of Photoscience
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    • 제9권2호
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    • pp.154-157
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    • 2002
  • Direct, continuous, and accurate measurements of solar ultraviolet irradiance (290-400 nm: UVR) have been carried out since 1990, by using both band-spectral ultraviolet-B (290-320 nm: UV-B) and ultraviolet-A (320-400 nm: UV-A) radiometers at Tokai University in Hiratsuka, Japan (35$^{\circ}$N, 139$^{\circ}$E). From our observations, the following findings are provided: 1) an increasing trend in solar UV -B from Oct. 1990 to Sept. 2000; 2) a regional comparison of solar UVR in Japan; 3) the distinct characteristics of UV-B and UV-A irradiance, such as diffuse property, daily and seasonal variation; and 4) human body protection against solar UVR. An increasing 10-year trend in global solar UV - B in Hiratsuka corresponded to a decrease in the total ozone amount measured at Tsukuba (36$^{\circ}$N, 140$^{\circ}$E), giving supportive evidence for a direct link between these two parameters. Furthermore, a strong correlation was found between solar UV-B and total ozone amount from results of UVR measurements at four Tokai University monitoring stations dispersed throughout Japan. Additional results revealed different diffuse properties in global solar UV and in global solar total (300-3000 nm: Total) irradiances. For example, in the global UVR, the diffuse component was dominant: about 80 % independent of weather, with more than 60 % of global UV-B, and more than 50% of global UV-A with even a cloudless clear sky. On the other hand, the portion of the diffuse in the global total irradiance was very low, less than 10 % on a cloudless clear day. Daily and seasonal variations of UV -B and UV -A irradiances were found to be quite different, because of the marked dependence of UV -B irradiance on the atmospheric ozone amount. Moreover, UV -B irradiance showed large daily and seasonal variations: the ratio between maximum and minimum irradiances was more than 5. In contrast, the variation in UV-A was small: the ratio between maximum and minimum was less than 2. Three important facts are proposed concerning solar UVR protection of the human body: 1) the personal minimal erythema dose (MED); 2) gender based difference in MED values; and 3) proper colors for UVR protective clothing.

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복숭아흑진딧물(Myzus persicae Sulzer)의 생활사에 관한 연구 (Studies on the life history of green peach aphid, Myzus persicae Sulzer (Homoptera))

  • 심재영;박중수;백운하;이영복
    • 한국응용곤충학회지
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    • 제16권3호
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    • pp.139-144
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    • 1977
  • 복숭아혹진딧물의 생활사를 야외에서 조사한 결과 1. 월동란은 3월하순-4월 상순에 부화하며, 부화율은 $95\%$였다. 2. 5월상, 중순에 겨울숙주로 이동하여 11월 상, 중순 사이에 월동란을 낳았다. 3. 1년간 빠른 개체군은 23세대, 늦은 개체군은 9세대를 영위하였다. 4. 성숙기간은 약 10.8일, 생식기간은 약 15.8일, 수명은 28.5일이었으며 모두 봄, 가을에는 그 기간이고 여름에는 짧은 경향을 나타내었다. 5. 암컷 한 마리당 총산자수는 약 50마리, 최다산자수는 118마리 이었으며, 1일 평균산자수는 3.2마리 이었으며 1일 최다산자수는 13마리었다.

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명료도 평가용 단음절 목록의 개발 (On the Development of Monosyllable Lists for Articulation Tests)

  • 김정환;강성훈;장대영;김천덕
    • 한국음향학회지
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    • 제13권4호
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    • pp.69-76
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    • 1994
  • 본 연구에서는 일상회화에서 103,581개의 단음절 목록을 표집하고, 한국에 특성에 맞는 5가지 선정규칙을 적용하여, 국내의 명료도 평가용 단음절 목록을 개발하였으며, 이를 이용하여 음성품질 열화요인에 대한 타당성 검증을 수행하였다. 한귀요인인 S/N비와 차단주파수 변화에 대한 타당성 검증결과, 명료도 점수는 열화요인에 따라 체계적인 변화를 보였다. 또한, 명료도 점수에 미치는 두귀요인의 영향을 살펴보기 위해, 방해잡음의 수평 제시각도 효과에 대해서도 검증한 결과, 명료도는 방해잡음의 수평 제시각도에 따라 변화하였다. 그리고, 모든 실험조건에서 목록간에 어떠한 유의미한 차이도 보이지 않았다.

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