• Korean Journal of Pharmacognosy
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• v.45 no.1
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• pp.84-87
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• 2014

A high performance liquid chromatography (HPLC) method for the quantitation of ellagic acid in Nymphaea tetragona was developed for the quality control of functional cosmetic ingredient, the extract of N. tetragona. Separation and quantitation were successfully achieved with a Kromasil C18 column ($5{\mu}m$, $250mm{\times}4.6mm$, i.d.) by isocratic elution of a mixture of acetonitrile containing 0.1% trifluoroacetic acid and water containing 0.03% phosphoric acid at a flow rate of 1.0 ml/min. The UV detector was used for the detection and the wavelength for quantitation was set at 254 nm. The presence of ellagic acid in the extract was determined by comparison of retention time and spiking with authentic standard. Analytical results showed good linearity ($R^2=0.99996$) in relatively wide concentration ranges. The R.S.D. for precision test was less than 3.0%. Recovery of the compound was 98.55~101.72% with R.S.D values less than 4.0%. In conclusion, this method has been successfully applied to the determination of ellagic acid in N. tetragona.

• Textile Coloration and Finishing
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• v.28 no.4
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• pp.290-298
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• 2016

In this study, the efficacy of water lily(Nymphaea tetragona) leaves as a new natural dye resource was investigated. For this purpose, the colorants from water lily leaves were extracted in methanol, evaporated, and powdered. Dyeing onto cotton fiber was carried out to study the effects of dyeing conditions, mordant type and mordanting method on dye uptake, color change, and colorfastness. FTIR analysis supported that hydrolyzable tannins and chrolophyll were contained in the extracted colorants. The colorants showed good affinity to cotton fiber showing Y Munsell color. Pre-mordanting method gave better results in terms of dye uptake than post-mordanting method. By mordanting, dyed fabrics exhibited various colors such as green, khaki, brownish yellow, dark brown, dark gray and so on. Colorfastness to washing and rubbing was relatively good showing 4-5 rating. The light fastness was improved 1-2 rating by Fe mordanting. The dyed cotton fabric showed antimicrobial activity.

• Journal of the Korean Magnetic Resonance Society
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• v.22 no.3
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• pp.54-63
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• 2018

Nymphaea tetragona, also known as waterlilies, is aquatic plant in the family of Nymphaeceae. Three flavonoids(3, 4, and 5) and one mixed flavonoids were separated from this plant. The mixed flavonoid consisted of two flavonoids; a well-known quercetin(1) and a new natural flavonoid(2). The latter also has two chiral centers and their configurations were established by ROESY experiment. Two glycoflavonoids were determined as isoquercetin and quercetin-3-O-${\beta}$-xyropyranosyl-($1{\rightarrow}2$)-${\beta}$-galactopyroside. The $^1H$ NMR spectra for 4 and 5 dissoloved in $DMSO-d_6$ solvent showed resonance proximity and and severely overlap in the glycoside region, hindering the determination of the configurations of the stereogenic centers of the sugar moieties. This problem was solved through the spin simulation. Here, the exact NMR parameters for the sugar moieties of 4 and 5 were listed.

• Textile Coloration and Finishing
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• v.29 no.3
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• pp.171-179
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• 2017

This study was to investigate the practicality and functionality of water lily(Nymphaea tetragona) leaves as a natural dye resource while searching for various dyeing methods to utilize them. Effect of dyeing condition including methanol ratio of dyebath, dyeing temperature and time, mordanting method, etc were investigated. Colorants were prepared by extraction in methanol and followed drying process. When composing 30% of methanol in the dyebath, better dyeuptake and uniform dyeing were resulted. Pre-mordanting method gave better results in terms of dye uptake than post-mordanting method on the wool fabrics. Fe and Ti were effective for increasing the dye uptake on the silk fabrics. Depending on mordant type and mordanting method, the dyed fabrics got various color showing green, khaki, brownish yellow, dark brown, dark gray and so on. As for color fastness, the silk fabrics dyed with water lily extract showed relatively high rating in light fastness(3~4, 4~5 rating), washing fastness(4~5, 5 rating), and rubbing fastness(4, 4~5 rating). The silk and wool fabrics dyed with water lily leaves extract showed excellent antimicrobial activity over 98% of bacterial reduction rate against Staphylococcus aureus and Klebsiella pneumoniae. It was confirmed that water lily leaves can be used as a natural dye resource for dyeing wool and silk fabrics because its colorants showed excellent affinity and antimicrobial functionality as well as good colorfastness.

• Journal of the Korean Applied Science and Technology
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• v.27 no.3
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• pp.249-256
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• 2010

A phytoremediation study has been conducted to see if some known aquatic plants can remove the pesticides, endosulfan-${\alpha},\;{\beta}$ and fenitrothion which are frequently used in the crop protection and golf course management, and are likely to exist as residual pollutants in the aquatic ecosystems. Among the five aquatic plants tested in the microcosms, water lily Nymphaea tetragona Georgi showed the highest degradation efficacies (85~95%) for the three pesticides as opposed to the control(13~26%). The efficacies for the other plants were in the range of 46~80% in the order of Pistia stratiotes, Cyperus helferi, Eichhornia crassipes, and Iris pseudoacorus. Fenitrothion, an organo-phosphorus pesticide, was much more vulnerable to the phytoremediation than the organo-chlorine pesticides, endosulfan-${\alpha}$ and endosulfan-${\beta}$. The kinetic rate constants ($min^{-1}$) for removal of the three pesticides were more than 10 times higher than the control (non-planting) in case of Nymphaea tetragona Georgi. This aquatic plant showed kinetic rate constants about 2 times as much as the lower kinetic rate constants shown by Iris pseudoacorus. The reason for the highest degradation efficacy of water lily would be that the plant can live in the sediment and possess roots and broad leaves which could absorb or accumulate and degrade more pollutants in association with microbes. These results indicate that some of the selected aquatic plants planted near the agricultural lands and wetlands could contribute to remediation of pesticides present in these places, and could be applicable to protection of the aquatic ecosystems.

• The Plant Pathology Journal
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• v.26 no.3
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• pp.293-293
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• 2010

• Journal of Wetlands Research
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• v.8 no.2
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• pp.65-74
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• 2006

A study of the vascular hydrophyte communities and biomass was undertaken in the Bigumdo wetlands, Shinangun, from March to November, 2005. As a result, the vegetation was divided into 14 communities. Among them, emergent hydrophytes consisted of 6 communities, including Miscanthus sacchariflorus community, Phragmites communis community, Typha angustata community, Leersia japonica community, Paspalum disticum var. indutum community, and Persicaria thunbergii community, floating hydrophytes 5 communities including Hydrocharis dubia community, Nelumbo nucifera community, Euryale ferox community, Trapa japonica community and Nymphaea tetragona var. angusta community, free-floating hydrophytes 2 commuinties including Lemna paucicostata community and Spirodela polyrhiza community, and submergent hydrophyte 1 community, including Myriophyllum verticillatum community. Biomass was the highest at emergent hydrophytes and decreased along the life form, in the order like floating hydrophytes, submergent hydrophyte and free-floating hydrophytes. In addition, hydrophytes in the Bigumdo wetland showed the typical vertical zonation pattern like a natural swamp. These results were considered that the wetland of Bigumdo was characterized by the typical structure of aquatic plant ecosystem.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.345-352
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• 2023

Antibacterial activity against foodborne bacteria (Bacillus cereus, Staphylococcus aureus, Salmonella Enteritidis) and inhibitory activity against murine norovirus, a human norovirus surrogate, of 28 extracts from plants endemic to Korea were investigated in this study. All plant extracts showed antibacterial activity only against gram-positive bacteria, B. cereus and S. aureus. Extracts from Callistemon speciosus and Nymphaea tetragona showed inhibition zones of 16.54 and 24.35 mm against B. cereus and S. aureus, respectively, presenting the highest antibacterial activities recorded in this study. Among all samples, Ardisia japonica extract at concentrations of 100 and 200 ㎍/mL showed the highest virucidal activities of 96.6 and 100.0%, respectively. Ardisia japonica, Duchesnea indica, Polygonum aviculare, and Geum japonicum extracts showed high antibacterial and virucidal activity simultaneously without Raw 264.7 cell cytotoxicity. These plant extracts may serve as potential antimicrobials to control foodborne infections.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.1
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• pp.41-50
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• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.27-53
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• 2013

This study is carried out to offer raw data and establish strategy for conservation and restoration of river ecosystem by objective surveying and analysing the flora and vegetation distributed in Hwang river, Gyeongsangnam-do. The flora identified in this site were 406 taxa including 95 families, 252 genera, 360 species, 3 subspecies, 40 varieties and 3 forms. The rare plants were 3 taxa including Sagittaria sagittifolia subsp. leucopetala, Hydrocharis dubia and Acorus calamus. The endemic plants were 4 taxa including Populus tomentiglandulosa, Salix koriyanagi, Paulownia coreana and Weigela subsessilis. The specific plants by floristic region were 17 taxa including Salix siuzevii, Poncirus trifoliata, Potamogeton maackianus and so forth. The naturalized plants were 39 taxa including Phytolacca americana, Chenopodium glaucum, Lepidium virginicum, Oenothera erythrosepala, Xanthium canadense, Lolium multiflorum and so forth. The invasive alien plants were Ambrosia artemisiifolia and Ambrosia trifida. The whole NI and UI were each 9.6% and 13.9%. The hydrophytes were 27 taxa including Marsilea quadrifolia, Nymphaea tetragona, Ceratophyllum demersum, Trapa japonica and so forth. In the results of growth forms of the hydrophytes, emergent species were 14 taxa, 3 taxa of floating-leaved species, 4 taxa of free-floating species and 6 taxa of submerged species. The dominant vegetations were Salix spp. community, Miscanthus sacchariflorus community, Phragmites japonica community and Phragmites communis community.