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Changes of Phytoplankton Community with Inflow of Sea Water in Gyoungpo Lake; Comparison between 1998 and 2012 (해수 유입량 변동으로 인한 경포호 식물플랑크톤 군집의 변화; 1998년과 2012년도의 비교)

  • Lee, Eun Joo;Lee, Kyu Song
    • Korean Journal of Ecology and Environment
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    • v.47 no.spc
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    • pp.48-56
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    • 2014
  • Weekly changes of water environments and phytoplankton community with the salinity gradients were investigated at Gyoungpo Lake from April to November in 1998 and 2012. Underwater crossam in Gyoungpo Lake was removed in 2004. Thereafter, average salinity of Gyoungpo lake increased from 7.5 ppt in 1998 to 20 ppt in 2012. A total of 99 and 80 species of phytoplankton was observed from the sampled in 1998 and 2012, respectively. The number of common species during the 2 separate years was 40. Transparency, SS, $NO_3-N$ concentration and N/P ratio in 2012 were lower than those in 1998. During the period of water shortage (April, May) of 2012 transparency decreased due to decreased salinity and increased SS and Chl. a. Correlation coefficients between species and community scores of DCA ordination based on data matrix of the phytoplankton revealed larger variation among sampling seasons in 1998 than in 2012. The increase of seawater influx and conversion rates following the removal of the underwater crossbeam might explain such a differential variation. Gymnodium sp., Peridinium sp., Prorocentrum sp., Nitzschia longissima, Schroederia setigera, Lyngbya sp., Asterococcus limneticus, Asterococcus superbus and Cyclotella meneghiniana were found to well adapt at the high salinities in 2012. Comparatively, Asterrionella formosa, Nitzschia frustulum, Chlorella ellipsoidea, Scenedesmus bijuga and Scenedesmus ellipsoideus were observed at lower salinities in 1998. Two quite contrasting phytoplankton communities were found in the two seasons of a year, spring with limited precipitation and summer, the flood season.

Effect of Adipic Acid on Growth of Psychrotrophic Kimchi Lactic Acid Bacteria and Its Effect on Mulkimchi Fermentation (김치 저온젖산균에 대한 아디프산의 항균효과 및 물김치 저장성 연장효과)

  • Kang, Kyung-Ja;Song, Hyuk-Hwan;Kim, Young-Bae;Jung, Dong-Hyo;Lee, Chan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.857-863
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    • 2004
  • The effects of adipic acid on the growth of psychrotrophic kimchi lactic acid bacteria and on the fermentatation of mulkimchi were investigated. Four Leuconostoc and one Lactobacillus species were isolated from kinnchi fermented for 50 days at 6$^{\circ}C$. The growth inhibition rate of adipic acid on psychrotrophic kimchi lactic acid bacteria and control strain, Leuconostoc mesenteroides KCCM l1324, was gradually increased from 0.1% of adipic acid concentration and its growth inhibition rate on selected strains reached 90% at 0.4% addition of adipic acid. On the bases of these results, the Preservative effect of adipic acid on the fermentation of mulkimchi was investigated for 25 days at 1$0^{\circ}C$. The pH of mulkimchi containing adipic acid was lower than that of control mulkimchi at the beginning of fermentation. However, the pH of the control mulkimchi decreased rapidly and the pH is lower at the end of fermentation than that of all samples containing adipic acid. The control increased rapidly during fermentation at the acidity. Adipic acid inhibited the growth of several microorganisms in mulkimchi including Lactobacilli. The number of Lactobacilli in control mutkimchi increased rapidly at the beginning stage of fermentation and it decreased at the end stage due to lowering of pH. However, that of mulkimchi with adipic acid slowly increased. Addition of 0.2% ethyl alcohol showed increase of preservative effect of 0.1% adipic acid in mulkimchi.

Fragment Analysis for Detection of the FLT3-Internal Tandem Duplication: Comparison with Conventional PCR and Sanger Sequencing (FLT3-ITD 검출을 위한 절편분석법: 일반 중합효소연쇄반응 및 직접염기서열분석법과의 비교)

  • Lee, GunDong;Kim, Jeongeun;Lee, SangYoon;Jang, Woori;Park, Joonhong;Chae, Hyojin;Kim, Myungshin;Kim, Yonggoo
    • Laboratory Medicine Online
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    • v.7 no.1
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    • pp.13-19
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    • 2017
  • Background: We evaluated a sensitive and quantitative method utilizing fragment analysis of the fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD), simultaneously measuring mutant allele burden and length, and verified the analytical performance. Methods: The number and allelic burden of FLT3-ITD mutations was determined by fragment analysis. Serial mixtures of mutant and wild-type plasmid DNA were used to calculate the limit of detection of fragment analysis, conventional PCR, and Sanger sequencing. Specificity was evaluated using DNA samples derived from 50 normal donors. Results of fragment analysis were compared to those of conventional PCR, using 481 AML specimens. Results: Defined mixtures were consistently and accurately identified by fragment analysis at a 5% relative concentration of mutant to wild-type, and at 10% and 20% ratios by conventional PCR and direct sequencing, respectively. No false positivity was identified. Among 481 AML specimens, 40.1% (193/481) had FLT3-ITD mutations. The mutant allele burden (1.7-94.1%; median, 28.2%) and repeated length of the mutation (14-153 bp; median, 49 bp) were variable. The concordance rate between fragment analysis and conventional PCR was 97.7% (470/481). Fragment analysis was more sensitive than conventional PCR and detected 11 additional cases: seven had mutations below 10%, three cases represented conventional PCR failure, and one case showed false negativity because of short ITD length (14 bp). Conclusions: The new fragment analysis method proved to be sensitive and reliable for the detection and monitoring of FLT3-ITD in patients with AML. This could be used to simultaneously assess ITD mutant allele burden and length.

Effect of amendments and their causes of rice yield increase in ill drained paddy soil (습답(濕沓)에 대(對)한 개량제(改良劑)의 효과(效果)와 유효개량제(有效改良劑)의 수도증수원인(水稻增收原因)에 관(關)한 연구(硏究))

  • Park, Chon Suh;Song, Jae Ha;Kim, Yung Sup;Lee, Chung Young;Choh, Young Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.4 no.1
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    • pp.13-19
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    • 1971
  • In order to establish the method of improving ill drained paddy soil where the accumulation of absorption inhibitor is worried in the earlier stages of rice growth, proper soil is selected and an field experiment is designed having treatments such as lime materials, none sulfate fertilizers, boron and straw etc. The data of yield and plant analysis in different stages of rice growth is eveluated and discussed to obtain following summaries. (1) Significant yield increase was made by the treatment of lime materials such as slacked lime or wollastonite powder, materials inhibiting the activity of microorganisms such as boron and of none sulfate fertilizers lacking inhibitor producing sources. (2) The crop scientifice causes of decreasing yield are the decreasing the number of panicles per hill, grains per panicle and the weight of grains. (3) The plant nutritional causes of decreasing yield are the lowering of nitrogen content throughout the life, phosphate content since young premodia formation stage of plant and the decreased content of magnesium, calcium and silicate in straw at harvesting stage. (4) The causes of lowering the content of various elements in rice plant grown in ill drained paddy soil are suggested as root damage by producing and accumulating absorption inhibitors such as organic acids and hydrogen sulfide etc, from the following observed facts; (a) In young premodia formation stage, attaining to the maximum production and accumulation of absorption inhibitor, the phosphate accumulation in plant was smaller in the phosphate plots than without phosphate plots and much higher in the neutralized plots by adding lime materials. (b) In the plots of straw addition, the potassium content in plant at the young premodia formation stage is very low probabley due to root damage by absorption inhibitor produced from the process of straw decomposition but higher at the stage of harvesting probably due to the immetabolic negative absorption of damaged roots. (c) The effect of boron, known as the inhibitor of microorganism activity to decompose organic matter, is apparent. (d) The effect of nonsulfate fertilizer treatment, having no source of producing inhibitor such as hydrogen sulfide, was significant. (e) All the yield components, decided around the young premodia formation stage attaining to the maximum inhibitor concentration in soil and minimum root activity, are significantly decreased.

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Proton Affinity Distributions of Humic Acid Extracted from Upland and Paddy Soils (논·밭토양으로부터 추출한 Humic Acid의 수소이온 친화력 분포)

  • Jeong, Chang-Yoon;Park, Chan-Won;Kim, Jeong-Gyu;Lim, Soo-Kil
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.4
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    • pp.429-439
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    • 1999
  • Potentiometric titration data were collected for some humic acids purified from Korean upland and paddy soils over a range of pH (3.0 - 11.0) with $NaNO_3$ background electrolyte concentrations (0.01, 0.10, 0.50 and 1.00 M). The data were applied to model A and V which included both intrinsic heterogeneity of humic materials and electrostatic interaction influences on binding sites. The elemental analysis were conducted for various type of humic samples. The $E_4/E_6$ ratio proposed negative correlation with the total carboxyl groups ($r^2$= 0.9988). The charge ($cmol_c\;kg^{-1}$) on the humic acids became more negative as the ionic strength increased. In both continuous and batch titrations, the ionic strength effect was greater in Namweon series (pH 6.39) than others at pH 5.00. The effect of ionic strength on surface charge appears to be greater in batch titrations. This could suggest that continuous titrations do not represent an equilibrium state and the effects of electrolyte concentration was not fully realized during the course of titrations. Both models described experimental data obtained from continuous and batch titrations well over a range of ionic strengths. Model A is more simpler than model V but adaptes more fitted parameters. Thus, the observed change in apparent binding constants with surface charge is regarded solely due to electrostatic influences rather than functional group heterogeneity. However, Model V is more mechanistically realistic in a number of discrete ligand binding sites.

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Introduction on the Products and the Quality Management Plans for GOCI-II (천리안 해양위성 2호 산출물 및 품질관리 계획)

  • Lee, Sun-Ju;Lee, Kyeong-Sang;Han, Tae Hyun;Moon, Jeong-Eon;Bae, Sujung;Choi, Jong-kuk
    • Korean Journal of Remote Sensing
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    • v.37 no.5_2
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    • pp.1245-1257
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    • 2021
  • GOCI-II, succeeding the mission of GOCI, was launched in February 2020 and has been in regular operation since October 2020. Korea Institute of Ocean Science and Technology (KIOST) processes and produces in real time Level-1B and 26 Level-2 outputs, which then are provided by Korea Hydrographic and Oceanographic Agency (KHOA). We introduced current status of regular GOCI-II operation and showed future improvement. Basic GOCI-II products including chlorophyll-a, total suspended materials, and colored dissolved organic matter concentration, are induced by OC4 and YOC algorithms, which were described in detail. For the full disk (FD), imaging schedule was established considering solar zenith angle and sun glint during the in-orbital test, but improved by further considering satellite zenith angle. The number of slots satisfying the condition 'Best Ocean' significantly increased from 15 to 78. GOCI-II calibration requirements were presented based on that by European Space Agency (ESA) and candidate fixed locations for calibrating local observation area were. The quality management of FD uses research ships and overseas bases of KIOST, but it is necessary to establish an international calibration/validation network. These results are expected to enhance the understanding of users for output processing and help establish detailed plans for future quality management tasks.

Prediction of Potential Species Richness of Plants Adaptable to Climate Change in the Korean Peninsula (한반도 기후변화 적응 대상 식물 종풍부도 변화 예측 연구)

  • Shin, Man-Seok;Seo, Changwan;Lee, Myungwoo;Kim, Jin-Yong;Jeon, Ja-Young;Adhikari, Pradeep;Hong, Seung-Bum
    • Journal of Environmental Impact Assessment
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    • v.27 no.6
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    • pp.562-581
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    • 2018
  • This study was designed to predict the changes in species richness of plants under the climate change in South Korea. The target species were selected based on the Plants Adaptable to Climate Change in the Korean Peninsula. Altogether, 89 species including 23 native plants, 30 northern plants, and 36 southern plants. We used the Species Distribution Model to predict the potential habitat of individual species under the climate change. We applied ten single-model algorithms and the pre-evaluation weighted ensemble method. And then, species richness was derived from the results of individual species. Two representative concentration pathways (RCP 4.5 and RCP 8.5) were used to simulate the species richness of plants in 2050 and 2070. The current species richness was predicted to be high in the national parks located in the Baekdudaegan mountain range in Gangwon Province and islands of the South Sea. The future species richness was predicted to be lower in the national park and the Baekdudaegan mountain range in Gangwon Province and to be higher for southern coastal regions. The average value of the current species richness showed that the national park area was higher than the whole area of South Korea. However, predicted species richness were not the difference between the national park area and the whole area of South Korea. The difference between current and future species richness of plants could be the disappearance of a large number of native and northern plants from South Korea. The additional reason could be the expansion of potential habitat of southern plants under climate change. However, if species dispersal to a suitable habitat was not achieved, the species richness will be reduced drastically. The results were different depending on whether species were dispersed or not. This study will be useful for the conservation planning, establishment of the protected area, restoration of biological species and strategies for adaptation of climate change.

In vitro Multiplication of Hosta Tratt. Species Native to Korea by Shoot-tip Culture (경정배양에 의한 한국 자생 비비추속 식물의 기내증식)

  • Choi, Han;Yang, Jong Cheol;Ryu, Sun Hee;Yoon, Sae Mi;Kim, Sang Yong;Lee, Seung Youn
    • Korean Journal of Plant Resources
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    • v.32 no.1
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    • pp.53-62
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    • 2019
  • The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

Characteristics Analysis of Snow Particle Size Distribution in Gangwon Region according to Topography (지형에 따른 강원지역의 강설입자 크기 분포 특성 분석)

  • Bang, Wonbae;Kim, Kwonil;Yeom, Daejin;Cho, Su-jeong;Lee, Choeng-lyong;Lee, Daehyung;Ye, Bo-Young;Lee, GyuWon
    • Journal of the Korean earth science society
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    • v.40 no.3
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    • pp.227-239
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    • 2019
  • Heavy snowfall events frequently occur in the Gangwon province, and the snowfall amount significantly varies in space due to the complex terrain and topographical modulation of precipitation. Understanding the spatial characteristics of heavy snowfall and its prediction is particularly challenging during snowfall events in the easterly winds. The easterly wind produces a significantly different atmospheric condition. Hence, it brings different precipitation characteristics. In this study, we have investigated the microphysical characteristics of snowfall in the windward and leeward sides of the Taebaek mountain range in the easterly condition. The two snowfall events are selected in the easterly, and the snow particles size distributions (SSD) are observed in the four sites (two windward and two leeward sites) by the PARSIVEL distrometers. We compared the characteristic parameters of SSDs that come from leeward sites to that of windward sites. The results show that SSDs of windward sites have a relatively wide distribution with many small snow particles compared to those of leeward sites. This characteristic is clearly shown by the larger characteristic number concentration and characteristic diameter in the windward sites. Snowfall rate and ice water content of windward also are larger than those of leeward sites. The results indicate that a new generation of snowfall particles is dominant in the windward sites which is likely due to the orographic lifting. In addition, the windward sites show heavy aggregation particles by nearby zero ground temperature that is likely driven by the wet and warm condition near the ocean.

Impact of Entrepreneurial Business Start-up Motivation, Entrepreneurial Spirit, and Entrepreneurial Competence Characteristics on Start-up Companies' Sustainability: Focusing on the Mediating Effect of the Start-up Companies' Business Performance (창업자의 창업동기, 창업가정신 그리고 창업가 역량특성이 창업기업 지속가능성에 미치는 영향: 창업기업 경영성과를 매개로 하여)

  • Hyuk, Kang Han;Park, Woojin;Yun, Bae byung
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.3
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    • pp.59-71
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    • 2019
  • The domestic unemployment rate is now seriously increasing due to the impact of the international trend of incurring high costs using only a small number of manpower in a situation where the business has been being reduced in size. The nation is taking start-up promotion policies as a way to overcome the problem, but with the advent of excessive entrepreneurs and their concentration on specific areas, their survival is in an unclear situation. Thus, this study was intended to provide a useful direction for successful start-ups by identifying the impact of entrepreneurial motivation and entrepreneurship, internal characteristics of entrepreneurs, and of competency characteristics, external characteristics of them, on sustainability through business performance. Thus, the purpose of this study was to provide a useful direction for successful start-ups by identifying the impact of entrepreneurial motivation and entrepreneurship, internal characteristics of entrepreneurs, competency, and external characteristics, on sustainability through business performance. The result of verifying the hypothesis has showed that entrepreneurial motivation, entrepreneurship, and competency characteristics had a positive effect on business performance, and on the other hand, the business performance had a positive effect on sustainability. In addition, in terms of the impact of entrepreneurial motivation, entrepreneurship, and competency characteristics on sustainability, all of them had mediating effects on business performance. It is obvious that studies on the factors affecting business performance and corporate sustainability up to now have been carried out by only collecting the independent variables of this study individually or two of them. so it is judged that if the study, which has verified the practical direction in general through the verification of entrepreneurs's internal and external characteristics from various angles, is performed more comprehensively along with the addition of background characteristics of entrepreneurs, such as characteristics of start-up preparation, etc., in the future, more in-depth results will be able to be obtained.