• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제27권4호
• /
• pp.289-300
• /
• 2001

Telomerase is a ribonucleoprotein that synthesizes telomere repeats. It has been reported that activation of telomerase was associtated with immortalization, proliferative activity and carcinogenesis. Recently, telomerase activity has been extensively studied in many kinds of malignant tumors for clinical diagnostic and/or prognostic utilities. In neuroblastoma, breast carcinoma, gastric carcinoma, non-small cell lung carcinoma, close relationship has been reported between high telomerase activity and lymph node metastasis, tumor aggressiveness and poor prognosis. The purpose of this study is to investigate the clinical implication of telomerase activity assay as an adjunctive factor in decision-making on neck node management, speedy pre-operative judging on histologic malignancy grading. Thus we performed semi-quantitative assay of telomerase activity using Telomerase PCR ELISA $kit^{(R)}$(Boeringer Manheim, Germany) and evaluated correlation between telomerase activity and tumor size, neck node metastasis, Anneroth malignancy score and influence of pre-operative chemotherapy on its activity in 27 cases of oral squamous cell carcinomas and 18 cases of normal oral epithelium. Also, correlation between telomerase activities and PCNA indices was evaluated. The results were obtained as follows: 1. The telomerase activities were detected in 24 specimens out of 27 oral squamous cell carcinoma specimens (88.9%) and in 5 specimens out of 18 normal oral epithelium specimens (27.8%). The mean value of telomerase activities was $0.9793{\pm}0.3428$ in 24 oral squamous cell carcinoma specimens and $0.4855{\pm}0.1117$ in 5 normal oral epithelium specimens. The positivity rate and mean value of telomerase activities in oral squamous cell carcinoma specimens were significantly higher than those of normal oral epithelium specimens (p<0.05). 2. There was no significant correlation between total Anneroth malignancy score and telomerase activity (p>0.05), but points of mitosis index and depth of invasion were significantly correlated with telomerase activities (p<0.05). 3. The positive immunohistochemical staining for PCNA(proliferating cell nuclear antigen) was observed in 26 specimens out of 27 oral squamous cell carcinoma specimens and mean value of PCNA indices of 26 specimens was $53.67{\pm}26.46$. PCNA indices were significantly correlated with telomerase activities (p<0.05). 4. The mean value of telomerase activities was significantly higher in pathologic T3/T4 group than in T1/T2 group (p<0.01). There was no significant difference of mean value of telomerase activities between pathologic neck node positive group and negative group (p> 0.05). Pre-operative chemotherapy significantly lowered the telomerase activities (p<0.05). The above results suggested telomerase activity could be used as diagnostic marker and adjunctive parameter for judging on histologic malignancy in oral squamous cell carcinoma.

• Animal Bioscience
• /
• 제36권2호
• /
• pp.200-208
• /
• 2023

Objective: Muscle acetylcholine receptors have five alpha subunits (α, β, δ, ε, or γ), and cholinergic receptor nicotinic gamma subunit (CHRNG) is the γ subunit. It may also play an essential role in biological processes, including cell differentiation, growth, and survival, while the role of CHRNG has not been studied in the literature. Therefore, the purpose of this study is to clarify the effect of CHRNG on the proliferation and differentiation of bovine preadipocytes. Methods: We constructed a CHRNG overexpression adenovirus vector and successfully overexpressed it on bovine preadipocytes. The effects of CHRNG on bovine preadipocyte proliferation were detected by Edu assay, cell counting Kit-8 (CCK-8), real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Western blot and other techniques. We also performed oil red O, RT-qPCR, Western blot to explore its effect on the differentiation of preadipocytes. Results: The results of Edu proliferation experiments showed that the number of EDU-positive cells in the overexpression group was significantly less. CCK-8 experiments found that the optical density values of the cells in the overexpression group were lower than those of the control group, the mRNA levels of proliferating cell nuclear antigen (PCNA), cyclin A2 (CCNA2), cyclin B1 (CCNB1), cyclin D2 (CCND2) decreased significantly after CHRNG gene overexpression, the mRNA levels of cyclin dependent kinase inhibitor 1A (CDKN1A) increased significantly, and the protein levels of PCNA, CCNB1, CCND2 decreased significantly. Overexpression of CHRNG inhibited the differentiation of bovine preadipocytes. The results of oil red O and triglyceride determination showed that the size and speed of lipid droplets accumulation in the overexpression group were significantly lower. The mRNA and protein levels of peroxisome proliferator activated receptor gamma (PPAR class="checkNonKBPoint">γ), CCAAT enhancer binding protein alpha (CEBPα), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN) decreased significantly. Conclusion: Overexpression of CHRNG in bovine preadipocytes inhibits the proliferation and differentiation of bovine preadipocytes.

• Journal of Gastric Cancer
• /
• 제11권3호
• /
• pp.173-179
• /
• 2011

Purpose: It is difficult to obtain biopsies from gastrointestinal stromal tumors (GISTs) prior to surgery because GISTs are submucoal tumors, despite being the most common nonepithelial neoplasms of the gastrointestinal tract. Unlike anatomic imaging techniques, PET-CT, which is a molecular imaging tool, can be a useful technique for assessing tumor activity and predicting the malignant potential of certain tumors. Thus, we aimed to evaluate the usefulness of PET-CT as a pre-operative prognostic factor for GISTs by analyzing the correlation between the existing post-operative prognostic factors and the maximum SUV uptake (SUVmax) of pre-operative 18F-fluoro-2-deoxyglucose (FDG) PET-CT. Materials and Methods: The study was conducted on 26 patients who were diagnosed with gastric GISTs and underwent surgery after being examined with pre-operative FDG PET-CT. An analysis of the correlation bewteen (i) NIH risk classification and the Ki-67 proliferation index, which are post-operative prognostic factors, and (ii) the SUVmax of PET-CT, which is a pre-operative prognostic factor, was performed. Results: There were significant correlations between (i) SUVmax and (ii) Ki-67 index, tumor size, mitotic count, and NIH risk group (r=0.854, 0.888, 0.791, and 0.756, respectively). The optimal cut-off value for SUVmax was 3.94 between "low-risk malignancy" and "high-risk malignancy" groups. The sensitivity and specificity of SUVmax for predicting the risk of malignancy were 85.7% and 94.7%, respectively. Conclusions: The SUVmax of PET-CT is associated with Ki-67 index, tumor size, mitotic count, and NIH classification. Therefore, it is believed that PET-CT is a relatively safe, non-invasive diagnostic tool for assessing malignant potential pre-operatively.

• 대한간호학회지
• /
• 제22권4호
• /
• pp.569-588
• /
• 1992

This study examined the relationship between the experience of stressful life events and somatic symptoms of urban women. Data were collected by interviewing 200 women from June to July 1, 1991. Vsable data work obtained from 162 of the women. Modified version of a stressful life events measurement tool developed p.5. Lee (1984) and the Somatic Discomfort Inventory by Wittenborn were used to measure the variables. Data were processed by an 5.p.5.5. program and analyzed. statistically for percentage, T-test, ANOYA and Pearson Correlation coefficient. Result of the Study are as follows : 1) The group total mean score of stressful life events was 92.66 $\pm$ 10.41. The higher scores in the $\boxDr$Test and school$\boxUl$ of the Extrapersonal factor, in the $\boxDr$Health problems$\boxUl$ of the Intrapersonal factor and $\boxDr$Conflict and differences within the family$\boxUl$ of the Interpersonal factor, factors. which a suggested by Neuman's model. 2) The group total mean score for somatic symptoms was 100.41$\pm$9.74. The higher scores were for the factors of $\boxDr$Fatigue (1.94)$\boxUl$, $\boxDr$Menopause (1.74)$\boxUl$, $\boxDr$Muscular system(1.67)$\boxUl$ and $\boxDr$Sleeping (1.67)$\boxUl$ 3) The mean scores of stressful life events were higher in the 40~60 age group, for middle school graduates(P＜.05), career women and those in nuclear families (P＞.05). 4) The mean scores of somatic symptoms were higher in the 45~60 age group, for middle school graduates, non career women and women with 5 or more children(P＜.05). 5) There was a positive correlation between the scores of stressful life events and somatic symptoms (r＝.585 P＜.05). The higher the level of stressful life events the higher the score of somatic symptoms, the results were consistent with the Extrapersonal, Intrapersonal and Interpersonal stress factors of Neuman's Health Care Systems. This research assessed the stressful life events of women, who play the most important role in the family for illness prevention and health promotion and suggested the importance of programs in the Primary Health Services to build basic coping resources.

• Journal of Periodontal and Implant Science
• /
• 제51권6호
• /
• pp.374-385
• /
• 2021

Purpose: The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy. Methods: Ligature-induced periodontitis was performed in 96 rats. The ligature was tied in the cervical area of the mandibular left first molar. The animals were randomly divided into 4 groups: 1) NT, no treatment; 2) SRP, scaling and root planning; 3) SRP/PLA, SRP followed by filling the periodontal pocket with placebo gel (PLA); and 4) SRP/ALN, SRP followed by filling the periodontal pockets with 1% ALN gel. Histomorphometric (percentage of bone in the furcation region [PBF]) and immunohistochemical (receptor activator of nuclear factor-κB ligand, osteoprotegerin, and tartrate-resistant acid phosphatase) analyses were performed. Data were statistically analyzed, with the threshold of statistical significance set at P≤0.05. Results: The SRP, SRP/PLA, and SRP/ALN groups presented a higher PBF than the NT group (P≤0.01) at 7, 15, and 30 days. The SRP/ALN group presented a higher PBF than the SRP/PLA group in all experimental periods, as well as a higher PBF than the SRP group at 15 and 30 days. No differences were observed in the immunohistochemical analyses (P>0.05 for all). Conclusions: Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis.

• 대한지역사회영양학회지
• /
• 제4권3호
• /
• pp.356-365
• /
• 1999

This study was carried out to evaluate high-school students' nutrient intakes according to the status of depressive trends, and to analyse the in physical and mental health status. The volunteers were 560 high school students selected from 8 provinces, but 511 students were analyzed. A was used for evaluation, The questions were composed in consideration of socio-demographic characteristics, physical health condition by CMI, mental condition assessed by a modified Zung's self-rating depression scale (SDS), and the evaluation of food consumption by a 24 hours recall method. The family situations of the subjects were mostly nuclear family(73%), agricultural work(53%), and having religion(53%). Only 3% of the students suffered depression where as 19% showed depression trendencies. The individual factors that affected their mental condition were family farming and consciousness of academic accomplishment. The health status counted with CMI was 6.2/20 scores. The non-depressed trend group of students had a lower score of CMI than others. The overall nutrient intakes of students were low compared with the Korean RDA for adolescents. The Mean Adequacy Ratio(MAR) of nutrient intakes was only $0.45{\pm}0.23$. Iron, vitamin A, and riboflavin intakes were significantly low and correlated with depressive trend score. The result from the regression analysis showed that CMI, study accomplishment, and vitamin A were the most significant factors influencing mental condition. We concluded that Korean rural high-school students were healthy in mental condition and showed lower rates of depression academic. For there more we could say that the intakes of iron and vitamin A also influenced mental health. Findings confirmed the need of becoming hopeful job of farming and better nutritional status. These situations would be necessary for students to ameliorate the quality of rural living.

• 한국미생물·생명공학회지
• /
• 제19권1호
• /
• pp.37-44
• /
• 1991

진핵세포 유전자의 기초대사발현의 조절계를 밝히기 위한 일환으로, 효모의 histidine생합성계 효소의 구조유전자 HIS5를 이용하였다. HIS5 유전자는 충분한 아미노산 조건하에서는 발현이 억제되어 비교적 높은 기초발현만을 하나, 어떤 아미노산이 결핍되면 탈억제되어 높은 발현량을 보이며 탈억제는 cis의 작용인자인 promoter상의 5'-TGACTC-3' 및 trans 작용인자 GCN4와 GCD17 GCN2등이 관여한다. trans 작용인자들에 의한 HIS5 유전자의 발현량의 변화를 간단하게 측정하기 위하여, HIS5 promoter와 repressible acid phoshates(APase)의 구조유전자중 promoter를 제거한 DNA단편을 연결시켜 HIS5-PHO5 융합유전자를 이용하였다. gcn2 및 gcn4 변이주의 APase 활성은 야생주와 비교하여 3내지 4배 낮았으며, gcn2변이주와 gcn2 gcn4 이중변이주의 APase 활성은 유사하였다.

• Tuberculosis and Respiratory Diseases
• /
• 제42권2호
• /
• pp.149-155
• /
• 1995

연구목적: Cytokeratin 19는 기관지의 상피세포와 같은 단순 또는 가중층상피세포에 국한된 40KD의 산성 분자로 면역조직학적 검사를 통해 cytokeratin 19가 폐암 조직에서 많이 발현되는 것으로 알려져 있다. Cytokeratin 19에 특징적인 단일 클론 항체 BM 19-21과 KS 19-1을 이용한 면역방사계수법, CYFRA 21-1을 이용하여 cytokeratin 19분절이 폐암 특히 편평상피세포암의 진단에 유용한 표지자가 될 수 있다는 보고가 있어 폐암 표지자로서 CYFRA 21-1의 유용성을 조사해 보기 위하여 본 연구를 하였다. 방법: 저자 등은 영남대학교 의과대학 부속병원 내과에 1993년 4월부터 1994년 8월까지 입원한 원발성 폐암 환자 39명(편평상피 세포암 19명, 선암 11명, 소세포암 9명)을 폐암군으로, 비악성 호흡기질환자 15명(폐결핵 8명, 만성 폐색성 폐질환 3명, 폐렴 2명, 만성 폐색성 폐질환과 폐결핵이 동반된 환자 2명)을 대조군으로 하여 새로운 폐암 표지자의 가능성이 있는 CYFRA 21-1의 유용성을 조사하였다. CYFRA 21-1의 측정은 면역방사계수측정 kit인 ELSA-CYFRA 21-1을 사용하였다. 결과: 폐암의 조직학적 분류에 따른 CYFRA 21-1의 혈중 측정치는 편평상피세포암이 $20.2{\pm}4.7ng/ml$, 선암이 $7.2{\pm}1.6ng/ml$, 비소세포암이 $15.5{\pm}4.7ng/ml$로 모두 대조군의 $1.7{\pm}0.5ng/ml$보다 유의하게 증가되어 있었다(p<0.01). 또한 비소세포암중 편평상피세포암에서 선암보다 유의하게 증가되어 있었다(p<0.05). 그러나 소세포암에서는 $2.9{\pm}0.9ng/ml$로 대조군과 유의한 차이가 없었다. CYFRA 21-1의 정상 범위를 3.3ng/ml 이내로 하였을때 소세포암에서는 민감도 11.1%, 특이도 65.2% 였으나, 비소세포암에서는 민감도 70.0%, 특이도 62.5%였고 이 중 편평상피 세포암인 경우 민감도 73.7%, 특이도 75%였으며 선암인 경우 63.6%, 78.9%로 산출되었다. 결론: CYFRA 21-1은 비소세포암의 종양 표지자로 유용성이 있을 것으로 생각되며, 특히 편평상피 세포암의 진단에 도움이 될 것으로 생각되었다.

• 한국수정란이식학회지
• /
• 제33권3호
• /
• pp.139-147
• /
• 2018

Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for animal cloning. Until now, SCNT has been inefficient, especially in dog. It is believed that an embryo developmental block in SCNT embryos is cause of low production efficiency. However, no studies have been performed on canines for embryo developmental block. In this study, we attempted to evaluate the beneficial role of EDTA in canine parthenogenic (PA) embryos development to overcome embryo developmental block. The PA embryos were divided into 0.01 mM EDTA treated and non-treated groups. Embryo developmental efficiency was measured by activating chemically parthenote. After EDTA induction, PA embryos were evaluated for embryonic development, Reactive Oxygen Species (ROS) activity, mitochondrial integrity, ATP production and genomic activation. The EDTA treated PA embryos showed significantly higher survival rate and improved cavity formation compared to non-treated. Furthermore, cytoplasmic ROS level was mitigated and mitochondrial membrane potential was found significantly higher in EDTA treated group followed by higher ATP production. Moreover, major embryonic genomic activation specific markers/factors were also elevated in EDTA treated group. Conclusively, we elucidated that EDTA showed substantially positive effect to overcome embryo developmental block in canine.

• 콘크리트학회논문집
• /
• 제25권5호
• /
• pp.497-508
• /
• 2013

이 논문에서는 화재 발생에 따른 구조물의 성능 변화를 평가하기 위한 철근콘크리트 부재의 수치해석모델이 제안되었다. 화재 발생 시 유발되는 전도, 대류 및 복사열의 효과를 고려한 비정상 열전달 해석을 수행하였으며 이를 통해 단면 내 온도분포를 결정하였다. 또한, 적층섬유단면을 적용하여 온도증가로 인해 단면 내 위치에 따라 달라지는 재료의 비선형성을 고려하였다. 이 때, 온도변화에 따라 유발되는 열팽창 변형률, 비정상상태 변형률, 크리프 변형률 등의 비역학적 변형특성을 단면 내 각 섬유에 대해 고려함으로써 화재 발생 시의 극심한 온도증가를 고려한 비선형 해석을 수행하였다. 제안된 해석모델의 타당성을 입증하기 위하여 철근콘크리트부재의 표준화재실험으로부터 얻어진 실험결과와 해석결과를 비교하였으며, 특히, 화재 시간에 따른 저항능력의 변화를 살펴봄으로써, 철근콘크리트 부재의 거동특성을 평가하고 이를 설계규준에서 제시하는 단면 및 저항능력과 비교하였다.