Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of EDTA on canine parthenote development during in vitro culture

개 parthenote in vitro culture시 EDTA 첨가에 의한 발달율 향상

  • Jeong, Haeyun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Zhao, Minghui (Qingdao Agricultural University) ;
  • No, Jin-Gu (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ullah, Imran (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Whi-Cheul (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Wi, Hayeon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hur, Tai-young (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Im, Gi-sun (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jong-Gug (Animal Reproduction Laboratory, College of Agriculture and Life Science, Chonbuk National University) ;
  • Lee, Seunghoon (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration)
  • 정해윤 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • ;
  • 노진구 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • ;
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 위하연 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 옥선아 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 우제석 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 허태영 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 임기순 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 김종국 (전북대학교 농업생명과학대학 가축번식학 연구실) ;
  • 이승훈 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2018.09.03
  • Accepted : 2018.09.21
  • Published : 2018.09.30
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Abstract

Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for animal cloning. Until now, SCNT has been inefficient, especially in dog. It is believed that an embryo developmental block in SCNT embryos is cause of low production efficiency. However, no studies have been performed on canines for embryo developmental block. In this study, we attempted to evaluate the beneficial role of EDTA in canine parthenogenic (PA) embryos development to overcome embryo developmental block. The PA embryos were divided into 0.01 mM EDTA treated and non-treated groups. Embryo developmental efficiency was measured by activating chemically parthenote. After EDTA induction, PA embryos were evaluated for embryonic development, Reactive Oxygen Species (ROS) activity, mitochondrial integrity, ATP production and genomic activation. The EDTA treated PA embryos showed significantly higher survival rate and improved cavity formation compared to non-treated. Furthermore, cytoplasmic ROS level was mitigated and mitochondrial membrane potential was found significantly higher in EDTA treated group followed by higher ATP production. Moreover, major embryonic genomic activation specific markers/factors were also elevated in EDTA treated group. Conclusively, we elucidated that EDTA showed substantially positive effect to overcome embryo developmental block in canine.

Keywords

References

  1. Bjerregaard B, Pedersen HG, Jakobsen AS, Rickords LF, Lai L, Cheong HT, Samuel M, Prather RS, Strejcek F, Rasmussen ZR et al. 2007 Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer. Molecular Reproduction and Development 74:35-41. https://doi.org/10.1002/mrd.20594
  2. Bolamba D, Russ KD, Olson MA, Sandler JL and Durrant BS. 2002. In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: Serum is not essential. Theriogenology 58:1689-1703. https://doi.org/10.1016/S0093-691X(02)01080-4
  3. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon F and Robl JM. 1998. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256-1258. https://doi.org/10.1126/science.280.5367.1256
  4. Cui XS, Jin YX, Shen XH, Lee JY, Lee HS, Yin XJ, Kong IK and Kim NH. 2006. Epidermal growth factor enhances meiotic resumption of canine oocytes in the presence of BSA. Theriogenology 66:267-274. https://doi.org/10.1016/j.theriogenology.2005.11.011
  5. Gandolfi F and Moor RM. 1987. Stimulation of early embryonic development in the sheep by co‐culture with oviduct epithelial cells. J. Reprod. Fertil. 81:23-28. https://doi.org/10.1530/jrf.0.0810023
  6. Gardner DK and Lane M. 1996. Alleviation of the "2-cell block'' and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters. Hum. Reprod. 11:2703-2712. https://doi.org/10.1093/oxfordjournals.humrep.a019195
  7. Gardner DK, Lane MW and Lane M. 2000. EDTA stimulates cleavage atage bovine embryo development in culture but inhibits blastocyst development and differentiation. Mol. Reprod. Dev. 53:256-261.
  8. Goddard MJ and Pratt HP. 1983. Control of events during early cleavage of the mouse embryo: an analysis of the '2-cell block'. J. Embryol. Exp. Morphol. 73:111-133.
  9. Hashimoto S, Minami N, Yamada M and Imai H. 2000. Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents. Mol. Reprod. Dev. 56:520-526. https://doi.org/10.1002/1098-2795(200008)56:4<520::AID-MRD10>3.0.CO;2-0
  10. Hatoya S, Sugiyama Y, Torii R, Wijewardana V, Kumagai D, Sugiura K, Kida K, Kawate N, Tamada H, Sawada T and Inaba T. 2006. Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes. Theriogenology 66:1083-1090. https://doi.org/10.1016/j.theriogenology.2005.12.015
  11. Kane MT and Foote RH. 1970. Culture of 2- and 4-cell rabbit embryos to the expanding blastocyst stage in synthetic media. Proc. Soc. Exp. Biol. Med. 133:921-925. https://doi.org/10.3181/00379727-133-34595
  12. Kim MJ, Oh HJ, Kim GA, Suh, HN, Jo YK, Choi YB, Kim DH, Han HJ and Lee BC. 2015. Altering histone acetylation status in donor cells with suberoylanilide hydroxamic acid does not affect dog cloning efficiency. Theriogenology 84:1256-1261 https://doi.org/10.1016/j.theriogenology.2015.07.006
  13. Kim MK, Fibrianto YH, Oh HJ., Jang G, Kim HJ, Lee KS, Kang SK, Lee BC and Hwang WS. 2004. Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected fromdogs with different stages of the estrus cycle. J. Vet. Sci. 5:253-258.
  14. Lee BC, Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Hossein MS, Kim JJ, Kang SK, Schatten G and Hwang WS. 2005. Dogs cloned from adult somatic cells. Nature 436:641. https://doi.org/10.1038/436641a
  15. Lee SK, Zhao MH, Kwon JW, Li YH, Lin ZL, Jin YX, et al. 2014. The association of mitochondrial potential and copy number with pig oocyte maturation and developmental potential. The Journal of reproduction and development 60:128-135. https://doi.org/10.1262/jrd.2013-098
  16. Lee SK, Zhao MH, Zheng Z, Kwon JW, Liang S, Kim SH, et al. 2015. Polymerase subunit gamma 2 affects porcine oocyte maturation and subsequent embryonic development. Theriogenology. 83(1):121-130. https://doi.org/10.1016/j.theriogenology.2014.08.019
  17. Lee SR, Kim BS, Kim JW, Kim MO, Kim SH, Yoo DH, Shin MJ, Park YS, Lee S, Park YB, Ha JH and Ryoo ZY. 2007. In vitro maturation, in vitro fertilization and embryonic development of canine oocytes. Zygote 15:347-353. https://doi.org/10.1017/S0967199407004352
  18. Liu Y, Li J, Lovendahl P, Schmidt M, Larsen K and Callesen H. 2015. In vitro manipulation techniques of porcine embryos: a meta-analysis related to transfers, pregnancies and piglets. Reprod. Fertil. Dev. 27:429-439. https://doi.org/10.1071/RD13329
  19. Maddox-Hyttel P, Svarcova O and Laurincik J. 2007. Ribosomal RNA and nucleolar proteins from the oocyte are to some degree used for embryonic nucleolar formation in cattle and pig. Theriogenology 68:(Suppl 1)S63-S70. https://doi.org/10.1016/j.theriogenology.2007.03.015
  20. Malekshah AK, Moghaddam AE and Daraka SM. 2006. Comparison of conditioned medium and direct co-culture of human granulosa cells on mouse embryo development. Indian. J. Exp. Biol. 44:189-192.
  21. Pirestani A, Hossieni SM, Moulavi F, Hajian M, Fourozanfar M and Esfahani MHN. 2011. In Vitro Bovine Embryo Development with Glucose and EDTA in Different Modifications of SOF Medium. IPCBEE 24:283-287.
  22. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A and Campbell KH. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90. https://doi.org/10.1038/35024082
  23. Rodrigues BA, dos Santos LC and Rodrigues JL. 2004. Embryonic development of in vitro matured and in vitro fertilized dog oocytes. Mol. Reprod. Dev, 67:215-223. https://doi.org/10.1002/mrd.10394
  24. Rodrigues BA, dos Santos LC and Rodrigues JL. 2006. The effect of hyaluronan concentrations in hST-supplemented TCM199 on in vitro nuclear maturation of bitch cumulus-oocyte complexes. Theriogenology 66:1673-1676. https://doi.org/10.1016/j.theriogenology.2006.01.015
  25. Rodrigues BA, dos Santos LC and Rodrigues JL. 2007. Effect of maturation medium on in vitro cleavage of canine oocytes fertilized with fresh and cooled homologous semen. Zygote 15:43-53. https://doi.org/10.1017/S0967199406003960
  26. Saikhun J, Sriussadaporn S, Thongtip N, Pinyopummin A and Kitiyanant Y. 2008. Nuclear maturation and development of IVM/IVF canine embryos in synthetic oviductal fluid or in co-culture with buffalo rat liver cells. Theriogenology 69:1104-10. https://doi.org/10.1016/j.theriogenology.2008.01.024
  27. Saint-Dizier M, Reynaud K and Chastant-Maillard, S. 2004. Chromatin, microtubules and kinases activities during meiotic resumption in bitch oocytes. Mol. Reprod. Dev. 68:205-212. https://doi.org/10.1002/mrd.20062
  28. Schultz RM. 1993. Regulation of zygotic gene activation in the mouse. Bioessays 15:531-538. https://doi.org/10.1002/bies.950150806
  29. Svarcova O, Laurincik J, Avery B, Mlyncek M, Niemann H and Maddox-Hyttel P. 2007. Nucleolar development and allocation of key nucleolar proteins require de novo transcription in bovine embryos. Molecular Reproduction and Development 74:1428-1435. https://doi.org/10.1002/mrd.20727
  30. Telford NA, Watson AJ and Schultz GA. 1990. Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol. Reprod. Dev. 26:90-100. https://doi.org/10.1002/mrd.1080260113
  31. Van Blerkom J and Davis P. 2007. Mitochondrial signaling and fertilization. Molecular Human Reproduction 13(11):759-770. https://doi.org/10.1093/molehr/gam068
  32. Wakayama T, Perry AC, Zuccotti M, Johnson KR and Yanagimachi R. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374. https://doi.org/10.1038/28615
  33. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KHS. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813. https://doi.org/10.1038/385810a0
  34. Wright RW Jr and Bondioli KR. 1981. Aspects of in vitro fertilization and embryos culture in domestic animals. J. Anim. Sci. 53:702. https://doi.org/10.2527/jas1981.533702x
  35. Zhao MH, Liang S, Kim SH, Cui XS and Kim NH. 2015. Fe(III) Is Essential for Porcine Embryonic Development via Mitochondrial Function Maintenance. PLoS ONE 10:e0130791. doi:10.1371/journal.pone.0130791.