• Nuclear Engineering and Technology
• /
• 제46권1호
• /
• pp.81-92
• /
• 2014

This study suggests 5 evaluation criteria (safety and technology, environmental impact, economic feasibility, social factors, and institutional factors) and 24 evaluation indicators for a NFC (nuclear fuel cycle) derived using factor analysis. To do so, a survey using 1 on 1 interview was given to nuclear energy experts and local residents who live near nuclear power plants. In addition, by conducting a factor analysis, homogeneous evaluation indicators were grouped with the same evaluation criteria, and unnecessary evaluation criteria and evaluation indicators were dropped out. As a result of analyzing the weight of evaluation criteria with the sample of nuclear power experts and the general public, both sides recognized safety as the most important evaluation criterion, and the social factors such as public acceptance appeared to be ranked as more important evaluation criteria by the nuclear energy experts than the general public.

• The Korean Journal of Physiology and Pharmacology
• /
• 제26권3호
• /
• pp.165-174
• /
• 2022

As the mechanism underlying glucose metabolism regulation by oxymatrine is unclear, this study investigated the effects of oxymatrine on pyroptosis in INS-1 cells. Flow cytometry was employed to examine cell pyroptosis and reactive oxygen species (ROS) production. Cell pyroptosis was also investigated via transmission electron microscopy and lactate dehydrogenase (LDH) release. Protein levels were detected using western blotting and interleukin (IL)-1β and IL-18 secretion by enzyme-linked immunosorbent assay. The caspase-1 activity and DNA-binding activity of nuclear factor kappa B (NF-κB) and nuclear factor (erythroid-derived 2)-like 2 protein (Nrf2) were also assessed. In the high glucose and high fat-treated INS-1 cells (HG + PA), the caspase-1 activity and LDH content, as well as Nod-like receptor family pyrin domain containing 3, Gsdmd-N, caspase-1, apoptosis-associated speck-like protein containing a CARD, IL-1β, and IL-18 levels were increased. Moreover, P65 protein levels increased in the nucleus but decreased in the cytoplasm. Oxymatrine attenuated these effects and suppressed high glucose and high fat-induced ROS production. The increased levels of nuclear Nrf2 and heme oxygenase-1 (HO-1) in the HG + PA cells were further elevated after oxymatrine treatment, whereas cytoplasmic Nrf2 and Keleh-like ECH-associated protein levels decreased. Additionally, the elevated transcriptional activity of p65 in HG + PA cells was reduced by oxymatrine, whereas that of Nrf2 increased. The results indicate that the inhibition of pyroptosis in INS-1 cells by oxymatrine, a key factor in its glucose metabolism regulation, involves the suppression of the NF-κB pathway and activation of the Nrf2/HO-1 pathway.

• Molecules and Cells
• /
• 제40권10호
• /
• pp.706-713
• /
• 2017

Osteoclasts are bone-resorbing cells that are derived from hematopoietic precursor cells and require macrophage-colony stimulating factor and receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) for their survival, proliferation, differentiation, and activation. The binding of RANKL to its receptor RANK triggers osteoclast precursors to differentiate into osteoclasts. This process depends on RANKL-RANK signaling, which is temporally regulated by various adaptor proteins and kinases. Here we summarize the current understanding of the mechanisms that regulate RANK signaling during osteoclastogenesis. In the early stage, RANK signaling is mediated by recruiting adaptor molecules such as tumor necrosis factor receptorassociated factor 6 (TRAF6), which leads to the activation of mitogen-activated protein kinases (MAPKs), and the transcription factors nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and activator protein-1 (AP-1). Activated NF-${\kappa}B$ induces the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), which is the key osteoclastogenesis regulator. In the intermediate stage of signaling, the co-stimulatory signal induces $Ca^{2+}$ oscillation via activated phospholipase $C{\gamma}2$ ($PLC{\gamma}2$) together with c-Fos/AP-1, wherein $Ca^{2+}$ signaling facilitates the robust production of NFATc1. In the late stage of osteoclastogenesis, NFATc1 translocates into the nucleus where it induces numerous osteoclast-specific target genes that are responsible for cell fusion and function.

• Biomolecules & Therapeutics
• /
• 제24권3호
• /
• pp.268-282
• /
• 2016

In the present study, we investigated the anti-inflammatory properties of Eucommia ulmoides Oliv. Bark. (EUE) in lipopolysaccharide (LPS)-stimulated microglial BV-2 cells and found that EUE inhibited LPS-mediated up-regulation of pro-inflammatory response factors. In addition, EUE inhibited the elevated production of pro-inflammatory cytokines, mediators, and reactive oxygen species (ROS) in LPS-stimulated BV-2 microglial cells. Subsequent mechanistic studies revealed that EUE suppressed LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs), phosphoinositide-3-kinase (PI3K)/Akt, glycogen synthase $kinase-3{\beta}$ ($GSK-3{\beta}$), and their downstream transcription factor, nuclear factor-kappa B ($NF-{\kappa}B$). EUE also blocked the nuclear translocation of $NF-{\kappa}B$ and inhibited its binding to DNA. We next demonstrated that EUE induced the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated heme oxygenase-1 (HO-1) expression. We determined that the significant up-regulation of HO-1 expression by EUE was a consequence of Nrf2 nuclear translocation; furthermore, EUE increased the DNA binding of Nrf2. In contrast, zinc protoporphyrin (ZnPP), a specific HO-1 inhibitor, blocked the ability of EUE to inhibit NO and $PGE_2$ production, indicating the vital role of HO-1. Overall, our results indicate that EUE inhibits pro-inflammatory responses by modulating MAPKs, PI3K/Akt, and $GSK-3{\beta}$, consequently suppressing $NF-{\kappa}B$ activation and inducing Nrf2-dependent HO-1 activation.

• Journal of international Conference on Electrical Machines and Systems
• /
• 제2권4호
• /
• pp.460-465
• /
• 2013

Electrical equipments of nuclear power plant are divided into class 1E and non-class 1E. Electrical equipment and systems that are essential to emergency reactor shutdown, containment isolation, reactor core cooling, and containment and reactor heat removal, are classified as class 1E. batteries of nuclear power plant are divided into four channels, which are physically and electrically separate and independent. The battery bank of class 1E DC power system of the nuclear power plant use lead-acid batteries in present. The lead acid battery, which has a high energy density, is the most popular form of energy storage. Kt factor of lead-acid battery is used to determine battery size and it is one of calculatiing coefficient for capacity. this paper analyzes Kt factor of lead-acid battery for the DC power system of nuclear power plant. In addition, correlation between Kt parameter and peukert's exponent of lead-acid battery for nuclear plant are discussed. The analytical results contribute to optimize of determining size Lead-acid battery bank.

• Nuclear Engineering and Technology
• /
• 제28권2호
• /
• pp.185-196
• /
• 1996

이 연구의 목적은 원자력발전소 페라이트 배관에 존재하는 원주방향 표면균열을 평가하는데 사용되는 ASME Code Z-Factor를 수정하는데 있다. ASME Code Z-Factor는 소성하중을 탄소성하중으로 보정하는 하중 보정 계수로서, 현재 사용되는 ASME Code Z-Factor는 최대하중을 과소평가하는 문제점이 있다. 이 연구에서는 먼저 기존의 SC. TNP방법이 수정되었으며, 그 이유는 기존의 SC. TNP 방법으로 예측된 최대허용하중이 실험에서 측정된 방법보다 약간 큰 결과를 주는 문제가 있기 때문이다. 이 수정된 SC. TNP 방법을 사용하여 페라이트 배관에 대한 새로운 Z-Factor를 개발하였다. 수정된 SC. TNP 방법의 타당성 과 새로 개발된 Z-Factor의 타당성을 원주방향 표면균열을 갖는 배관에 대한 실험 결과를 통해 조사하였다. 평 가결과는 수정된 SC. TNP 방법은 페라이트 및 오스테나이트 배관의 원주 방향 표면균열의 거동을 잘 예측할 수 있음을 보여 주며, 또한 수정된 SC. TNP 방법으로 구한 새로운 Z-Factor는 페라이트 배관에 존재하는 원주방향 표면균열의 거동을 잘 예측할 수 있음을 보여준다.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1996년도 추계학술발표회논문집(1)
• /
• pp.242-247
• /
• 1996

Flow oscillations in boiling channels induces a drastic reduction of the (critical heat flux) CHF or premature burnout. However, most of CHF works and correlations have been focused on stable flow conditions without considering flow oscillation. Therefore to improve the understanding on flow oscillation CHF, in this paper a new CHF correction factor to predict the CHF values under flow oscillation conditions has been developed from 126 experimental data. Also to investigate the dominant factor on flow oscillation CHF parametric trends are analyzed by using the developed correction factor. The overall mean accuracy ratio of the developed correction factor is 1.033 with a standard deviation of 0.195. The RMS errors 0.198. Its assessment shows that the predictions agree well with the experimental data within 25% error bounds.

• Reproductive and Developmental Biology
• /
• 제29권2호
• /
• pp.101-108
• /
• 2005

Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is a well-known inducer of apoptotic cell death in many tumor cells. 1RAIL is expressed in human placenta, and cytotrophoblast cells express 1RAIL receptors. However, the role of TRAIL in human placentas and cytotrophoblast cells is not. well understood. In this study a trophoblast cell line, JEG-3, was used as a model system to examine the effect of TRAIL. on key intracellular signaling pathways involved in the control of trophoblastic cell apoptosis and survival JEG-3 cells expressed receptors for 1RAIL, death receptor (DR) 4, DR5, decoy receptor (OcR) 1 and DeR2. Recombinant human TRAIL (rhTRAIL) did not have a cytotoxic effect determined by MIT assay and did not induce apoptotic cell death determined by poly-(ADP-ribose) polymerase cleavage assay. rhTRAIL induced a rapid and transient nuclear translocation of nuclear $factor-{\kappa}B(NF-{\kappa}B)$ determined by immunoblotting using nuclear protein extracts. rhTRAIL rapidly activated extracellular signal-regulated protein kinase (ERK) 1/2 as determined by immnoblotting for phospho-ERK1/2. However, c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (p38MAPK) and Akt (protein kinase B) were not activated by rhTRAIL. The ability of 1RAIL to induce $NF-{\kappa}B$ and ERK1/2 suggests that interaction between TRAIL and its receptors may play an important role in trophoblast cell function during pregnancy.

• 대한의생명과학회지
• /
• 제20권2호
• /
• pp.56-61
• /
• 2014

This study attempted to confirm the antioxidative potential of luteolin against tert-butyl hydroperoxide (t-BHP) induced oxidative damage and to investigate its molecular mechanism related to glutathione (GSH)-dependent enzymes in HepG2 cells. Treatment with luteolin resulted in attenuation of t-BHP induced generation of reactive oxygen species (ROS) and oxidative stress-mediated cell death. In addition, accelerated expression of GSH-dependent antioxidative enzymes, glutathione peroxidase (GPx) and glutathione reductase (GR), and heme oxygenase (HO)-1, as well as strengthened GSH content was induced by treatment with luteolin, which was in accordance with increased nuclear translocation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), a transcription factor for phase 2 enzymes, in a dose-dependent manner. These results suggest that the cytoprotective potential of luteolin against oxidative damage can be attributed to fortified GSH-mediated antioxidative pathway and HO-1 expression through regulation of Nrf2 in HepG2 cells.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1999년도 추계학술발표회요약집
• /
• pp.378.1-378
• /
• 1999