• Journal of Animal Science and Technology
• /
• v.54 no.6
• /
• pp.411-418
• /
• 2012

KIT gene is the major causative gene for coat color variation in diverse animal species. This gene regulates melanocyte migration from the neural crest to target tissues and the mutation of this gene can affect dominant white phenotypes in animals. Because this gene has a major influence for the coat color variation, single nucleotide polymorphisms (SNPs) in 14 Korean cattle (Hanwoo) and 5 Holstein individuals were investigated. The Hanwoo DNA samples included three different colored (5 Black, 5 Yellow and 4 Stripe) animals. Total 126 polymorphisms have been identified and 23 of them are located in the exon region. Also, 5 bp (TTCTC) and 3 bp (TCT) intronic indels in intron 3 and intron 5, respectively, were identified. Out of 23 exonic polymorphisms, 15 SNPs are the missense mutations and the rest of the SNPs are silence mutations. The neighbor-joining phylogenetic tree was constructed for the different colored animals using the obtained KIT gene sequences. Holstein breed showed a clear breed-specific cluster in the phylogenetic tree which is differed from Hanwoo. Also, three colored Hanwoo animals were not discriminated among the breeds. The KIT gene polymorphisms identified in this study will possibly give some solutions for the color variations in cattle with further verifications.

• Korean Journal of Environmental Agriculture
• /
• v.11 no.1
• /
• pp.20-25
• /
• 1992

To understand the fundamental features of reservoir environment and its future aspects, a simple predictive model for water quality was attempted with the aid of data easily obtained, Based on the data from 12 reservoirs in Korea, application of the simple predictive model was successfully made by means of statistical methods and simple physical submodels. Significant information on th effects of retention time on primary production in a reservoir, longitudinal change in water quality affected by certain non-dimensional parameters were also obtained. The chlorophyll-a concentration can be predicted by the equation as ; chlorophyll-a=($395{\time}limiting$ nutrient concentration) - 1.090.

• Proceedings of KOSOMES biannual meeting
• /
• 2006.05a
• /
• pp.47-52
• /
• 2006

The cultural grounds of laver has been surveyed using SPOT-5 satellite images to calculate the facilities of laver cultivation area in the coastal waters of Korea 10m resolution multispectral images of SPOT-5 are adopted for the south area of Daebu Island, Hwaseong city to develop an automatic detection approach of laver nets that consists of the following: band difference technique, canny edge detector and morphological analysis. The satellite-based facilities number was relatively high as compared with the licensed number in 2005, 676,749 chaek and 572,745 chaek(柵, unit of measure for laver farm), respectively. The data could be applied to achieve a good harvest for laver seaweed growers and to control its national production keeping a stable market price for the government body.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.10
• /
• pp.1599-1605
• /
• 2015

The development of rapid and efficient genome sequencing methods has enabled us to study the evolutionary background of bacterial genetic information. Here, we present comparative genomic analysis of 17 Streptomyces species, for which the genome has been completely sequenced, using the pan-genome approach. The analysis revealed that 34,592 ortholog clusters constituted the pan-genome of these Streptomyces species, including 2,018 in the core genome, 11,743 in the dispensable genome, and 20,831 in the unique genome. The core genome was converged to a smaller number of genes than reported previously, with 3,096 gene families. Functional enrichment analysis showed that genes involved in transcription were most abundant in the Streptomyces pan-genome. Finally, we investigated core genes for the sigma factors, mycothiol biosynthesis pathway, and secondary metabolism pathways; our data showed that many genes involved in stress response and morphological differentiation were commonly expressed in Streptomyces species. Elucidation of the core genome offers a basis for understanding the functional evolution of Streptomyces species and provides insights into target selection for the construction of industrial strains.

• The Bulletin of The Korean Astronomical Society
• /
• v.39 no.2
• /
• pp.95-95
• /
• 2014

The mass measurement of neutron stars or black holes is of fundamental importance in our understanding of the evolution of massive stars and core-collapse supernova explosions as well as some exotic physics of the extreme conditions. Despite the importance, however, it's very difficult to measure mass of these objects directly. One way to do this, if they are in binary systems, to measure their binary motions (i.e., Doppler shifts) which can give us direct information on their mass. Recently many new highly-obscured massive X-ray binaries have been discovered by new hard X-ray satellites such as INTEGRAL and NuSTAR. The new highly-obscured massive X-ray binaries are faint in the optical, but bright in the infrared with many emission lines. Based on the near-infrared spectroscopy, one can first understand the nature of stellar companions to the compact objects, determining its spectral types and luminosity classes as well as mass losses and conditions of (potential) circumstellar material. Next, spectroscopic monitoring of these objects can be used to estimate the mass of compact objects via measuring the Doppler shifts of the lines. For the former, broad-band spectroscopy is essential; for the latter, high-resolution spectroscopy is critical. Therefore, IGRINS appears to be an ideal instrument to study them. An IGRINS survey of these new highly-obscured massive X-ray binaries can give us a rare opportunity to carry out population analyses for understanding the evolution of massive binary systems and formation of compact objects and their mass ranges. In this talk, we will present a sample near-infrared high resolution spectra of HMXB, IGR J19140+0951 and discuss about its spectral feature. These spectra are obtained on 13th July, 2014 from IGRINS commissioning run at McDonald 2.7m telescope. And at final, we will introduce the upgrade plan of IGRINS Operation Software (IGOS), to gather the input from IGRINS observer.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.28 no.5
• /
• pp.1164-1171
• /
• 1999

In this study, we investigated the effects of obesity index on the risk factors of chronic diseases in obese children. The subjects were male and female children aged 9 to 12. The average obesity index was 147.7$\pm$13.8% in boys and 147.6$\pm$16.5% in girls. The average percentages of body fat was significantly higher in boys(33.9$\pm$5.7%) than in girls(30.7$\pm$4.4%). It was found that 26% of subjects had abnormal serum GOT and GPT values. Percentages of subjects at risk of cardiovascular disease(CVD) based on corresponding criteria of TG, T chol, HDL chol, and LDL chol were 28.6%, 28.6%, 45.5% and 42.7%, respectively. The risk of CVD was increased with the degree of obesity, which was not significant. The AI(atherogenic index) was higher in boys(4.6$\pm$2.3) than in girls(3.6$\pm$1.2). The serum T chol and LDL chol levels of subjects whose mother are in thirties were significantly higher than those of subjects whose mother are in fourties. The T chol levels of subjects who had mother with job were significantly higher compaired to those of subjects who had housewife mother. The results indicated the urgent need of nu tritional management for the obese children to prevent further devel opment of childhood obesity and hyperlipidemia. Based upon this study, devising method and media for the mother nutritional education as well as for the children nutritional education is required for better growth and health promotion of primary school children.

• BMB Reports
• /
• v.47 no.5
• /
• pp.262-267
• /
• 2014

Bcl-2 interacting cell death suppressor (Bis) has been shown to have anti-apoptotic and anti-stress functions. Recently, increased Bis expression was reported to correlate with glioma aggressiveness. Here, we investigated the effect of Bis knockdown on the acquisition of the invasive phenotype of A172 glioma cells, induced by 12-O-Tetradecanoylphorbol-3-acetate (TPA), using a Transwell assay. Bis knockdown resulted in a significant decrease in the migration and invasion of A172 cells. Furthermore, Bis knockdown notably decreased TPA-induced matrix metalloproteinase-9 (MMP-9) activity and mRNA expression, as measured by zymography and quantitative real time PCR, respectively. A luciferase reporter assay indicated that Bis suppression significantly down-regulated NF-${\kappa}B$-driven transcription. Finally, we demonstrated that the rapid phosphorylation and subsequent degradation of $I{\kappa}B-{\alpha}$ induced by TPA was remarkably delayed by Bis knockdown. These results suggest that Bis regulates the invasive ability of glioma cells elicited by TPA, by modulating NF-${\kappa}B$ activation, and subsequent induction of MMP-9 mRNA.

• BMB Reports
• /
• v.46 no.11
• /
• pp.533-538
• /
• 2013

The expression of matrix metalloproteinases (MMPs) produced by cancer cells has been associated with the high potential of metastasis in several human carcinomas, including breast cancer. Several pieces of evidence demonstrate that protein tyrosine phosphatases (PTP) have functions that promote cell migration and metastasis in breast cancer. We analyzed whether PTP inhibitor might control breast cancer invasion through MMP expression. Herein, we investigate the effect of 4-hydroxy- 3,3-dimethyl-2H benzo[g]indole-2,5(3H)-dione (BVT948), a novel PTP inhibitor, on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. The expression of MMP-9 and cell invasion increased after TPA treatment, whereas TPA-induced MMP-9 expression and cell invasion were decreased by BVT948 pretreatment. Also, BVT948 suppressed NF-${\kappa}B$ activation in TPA-treated MCF-7 cells. However, BVT948 didn't block TPA-induced AP-1 activation in MCF-7 cells. Our results suggest that the PTP inhibitor blocks breast cancer invasion via suppression of the expression of MMP-9.

• Fashion & Textile Research Journal
• /
• v.6 no.4
• /
• pp.503-510
• /
• 2004

The study is one of fundamental researches for the development of future smart clothing and textile products with blocking properties from electromagnetic waves by analyzing human physical symptoms in using electromagnetic products in such an environments. Among various textiles in the experiment, nano silver has shown the best blocking performance from electromagnetic waves, which decreases depending on the distance. The power spectrum distribution and the incidence of electroencephalogram between blocking materials and aural stimuli has shown that, ${\beta}$, wave appeared to be active in all channels except for $T_4$, whereas all waves appeared with processed materials and especially with nano silver silk(NSS), ${\alpha}$, ${\beta}$, ${\theta}$, ${\gamma}$ waves appeared active in all regions. As for the brain mapping of ${\alpha}$ wave according to time, there found a strong activity in $P_3$, $P_4$ of the parietal lobe, with all materials on all time regions. With silk nylon metal(SNM) and NSS, it appeared strong in $F_3$, $F_4$ as well. As for ${\beta}$, wave, the activity appeared strong in frontal lobe before 7min. 30sec, where it tends to diminish abruptly in 7min. 30sec. to 13min. 30sec. region. After 13min., it regained gradually. With NSS, it appeared strong in all areas except for the farthest $T_4$. The appearance of ${\nu}$ wave can be deduced as it can affect human body with its toxic property while the silver particles become nano-sized. Therefore, the study conducted with human participants requires a proper particle size of it which would not penetrate cellular tissues and a proper binder and binding treatment for it, to prevent the physical fatigues and the potential diseases. However, it is highly required for back-up researches to verify various aspects in applying nano silver to textile products.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.3 no.2
• /
• pp.199-205
• /
• 2000

Autoimmune enteropathy is a rare chronic diarrheal disease of infancy. Clinicopathologically, this entity is characterized by chronic secretory diarrhea, villous atrophy with crypt hypoplasia of a small intestine and/or associated autoimmune disorders, and absence of severe immunodeficiency. For the confirmation of diagnosis, antienterocyte autoantibody should be delineated. The treatment of choice of this disorder is immunosuppression. We has been experienced a case of autoimmune enteropathy without autoimmune disorders in a 10-month-old male infant. He developed protracted diarrhea from 5 months of his age and has been appeared to be failure to thrive. Antienterocyte autoantibody was demonstrated by immunohistochemistry and western blotting. He was successfully treated with corticosteroid and FK506. This is the first case report of autoimmune enteropathy without autoimmune disorders in Korea.