• Wind and Structures
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• 제17권3호
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• pp.317-343
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• 2013

When evaluating flutter instability, it is often assumed that incident wind is normal to the longitudinal axis of a bridge and the flutter critical wind speed estimated from this direction is most unfavorable. However, the results obtained in this study via oblique sectional model tests of four typical types of bridge decks show that the lowest flutter critical wind speeds often occur in the yaw wind cases. The four types of bridge decks tested include a flat single-box deck, a flat ${\Pi}$-shaped thin-wall deck, a flat twin side-girder deck, and a truss-stiffened deck with and without a narrow central gap. The yaw wind effect could reduce the critical wind speed by about 6%, 2%, 8%, 7%, respectively, for the above four types of decks within a wind inclination angle range between $-3^{\circ}$ and $3^{\circ}$, and the yaw wind angles corresponding to the minimal critical wind speeds are between $4^{\circ}$ and $15^{\circ}$. It was also found that the flutter critical wind speed varies in an undulate manner with the increase of yaw angle, and the variation pattern is largely dependent on both deck shape and wind inclination angle. Therefore, the cosine rule based on the mean wind decomposition is generally inapplicable to the estimation of flutter critical wind speed of long-span bridges under skew winds. The unfavorable effect of yaw wind on the flutter instability of long-span bridges should be taken into consideration seriously in the future practice, especially for supper-long span bridges in strong wind regions.

• Genomics & Informatics
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• 제19권2호
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• pp.17.1-17.13
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• 2021

Breast cancer is one of the leading causes of cancer in women all over the world and accounts for ~25% of newly observed cancers in women. Epigenetic modifications influence differential expression of genes through non-coding RNA and play a crucial role in cancer regulation. In the present study, epigenetic regulation of gene expression by in-silico analysis of histone modifications using chromatin immunoprecipitation sequencing (ChIP-Seq) has been carried out. Histone modification data of H3K4me3 from one normal-like and four breast cancer cell lines were used to predict miRNA expression at the promoter level. Predicted miRNA promoters (based on ChIP-Seq) were used as a probe to identify gene targets. Five triple-negative breast cancer (TNBC)-specific miRNAs (miR153-1, miR4767, miR4487, miR6720, and miR-LET7I) were identified and corresponding 13 gene targets were predicted. Eight miRNA promoter peaks were predicted to be differentially expressed in at least three breast cancer cell lines (miR4512, miR6791, miR330, miR3180-3, miR6080, miR5787, miR6733, and miR3613). A total of 44 gene targets were identified based on the 3'-untranslated regions of downregulated mRNA genes that contain putative binding targets to these eight miRNAs. These include 17 and 15 genes in luminal-A type and TNBC respectively, that have been reported to be associated with breast cancer regulation. Of the remaining 12 genes, seven (A4GALT, C2ORF74, HRCT1, ZC4H2, ZNF512, ZNF655, and ZNF608) show similar relative expression profiles in large patient samples and other breast cancer cell lines thereby giving insight into predicted role of H3K4me3 mediated gene regulation via the miRNA-mRNA axis.

• The Korean journal of internal medicine
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• 제33권6호
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• pp.1103-1110
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• 2018

Background/Aims: Several epidemiological studies have validated the association of interleukin gene polymorphisms with acute pancreatitis (AP) in different populations. However, there have been few studies in Asian ethnic groups. We aimed to investigate the relationships between inflammatory cytokine polymorphisms and AP as pilot research in a Korean ethnic group. Methods: Patients who had been diagnosed with AP were prospectively enrolled. DNA was extracted from whole blood, and DNA sequencing was subsequently performed. Single-nucleotide polymorphisms (SNPs) of the interleukin $1{\beta}$ (IL1B), interleukin 1 receptor antagonist (IL1RN), and tumor necrosis factor ${\alpha}$ (TNFA) genes of patients with AP were compared to those of normal controls. Results: Between January 2011 and January 2013, a total of 65 subjects were enrolled (40 patients with AP vs. 25 healthy controls). One intronic SNP (IL1RN -1129T>C, rs4251961) was significantly associated with the risk of AP (odds ratio, 0.304; 95% confidence interval, 0.095 to 0.967; p = 0.043). However, in our study, AP was not found to be associated with polymorphisms in the promoter regions of inflammatory cytokine genes, including IL1B (-118C>T, c47+242C>T, +3954C/T, and -598T>C) and TNFA (-1211T>C, -1043C>A, -1037C>T, -488G>A, and -418G>A). Conclusions: IL1RN -1129T>C (rs4251961) genotypes might be associated with a significant increase of AP risk in a Korean ethnic group.

• Molecules and Cells
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• 제42권2호
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• pp.123-134
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• 2019

Lysophosphatidic acid (LPA) is an endogenous lysophospholipid with signaling properties outside of the cell and it signals through specific G protein-coupled receptors, known as $LPA_{1-6}$. For one of its receptors, $LPA_1$ (gene name Lpar1), details on the cis-acting elements for transcriptional control have not been defined. Using 5'RACE analysis, we report the identification of an alternative transcription start site of mouse Lpar1 and characterize approximately 3,500 bp of non-coding flanking sequence 5' of mouse Lpar1 gene for promoter activity. Transient transfection of cells derived from mouse neocortical neuroblasts with constructs from the 5' regions of mouse Lpar1 gene revealed the region between -248 to +225 serving as the basal promoter for Lpar1. This region also lacks a TATA box. For the region between -761 to -248, a negative regulatory element affected the basal expression of Lpar1. This region has three E-box sequences and mutagenesis of these E-boxes, followed by transient expression, demonstrated that two of the E-boxes act as negative modulators of Lpar1. One of these E-box sequences bound the HeLa E-box binding protein (HEB), and modulation of HEB levels in the transfected cells regulated the transcription of the reporter gene. Based on our data, we propose that HEB may be required for a proper regulation of Lpar1 expression in the embryonic neocortical neuroblast cells and to affect its function in both normal brain development and disease settings.

• 스마트미디어저널
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• 제7권4호
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• pp.44-51
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• 2018

본 연구는 이동 및 휴대가 용이함과 더불어 지표로 부터 0~1km 높이의 특정한 위치에서의 환경정보를 수집하기 위하여 드론을 이용하였으며, 드론에 장착할 수 있는 대기환경 정보 수집기를 만들어 대기 중의 환경정보를 모니터링 하는 방안을 제안한다. 기상적인 요소로 온도와 습도를 측정하고, 환경적인 요소로 미세먼지와 $CO_2$를 관측하여 정상적인 값을 받을 수 있도록 하였다. 측정을 통하여 특정 기업, 공단, 지역의 대기가스 배출현황을 모니터링 함으로써 오염요인을 줄이는 정책수립에 도움을 줄 수 있도록 하였다. 그 동안 배기가스 검출은 굴뚝에 설치된 구멍에 검시자가 직접 접근하여 측정하다보니 안전상 많은 위험에 노출되었으나 제안하는 드론을 이용하게 되면 안정성 확보와 더불어 광범위한 정보수집이 가능하여 보다 정확한 정보수집과 다양한 산업적 활용이 가능함을 확인하였다.

• 디지털융복합연구
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• 제17권3호
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• pp.221-226
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• 2019

뇌파 파형중 베타파를 이용한 인간의 인지상태를 판별한다. 베타파는 인간의 인지상태중 스트레스 영역에 해당하는 특성이 있고, 이 영역에서 스트레스의 오버대역폭을 추출하기 위해서 저대역폭과 고대역폭 사이의 베타파간 상관관계를 분석해야 한다. 그러므로 본 논문에서는 효과적으로 베타파 상관관계를 분석하고 추출하기 위해 비지도학습 머신러닝을 이용한 Kmean 클러스터링 분석모델을 제시한다. 제시된 모델은 베타파 영역을 유사한 영역의 클러스터 군으로 분류하고 해당 클러스터링 범주에서 이상파형을 판별한다. 이상파형 판별을 위해 클러스터군의 밀집도와 정상범주 이탈영역을 기준으로 스트레스 위험군을 판별하고 판별된 스트레스 위험군에 대한 대처방안을 제공할 수 있다. 제시된 모델을 활용하면 뇌파파형을 통한 인지상태의 스트레스 지수분별이 가능하고, 개인의 인지상태에 대한 관리 및 응용이 가능하다. 또한 스트레스와 오피스증후군을 갖는 사람들에게 뇌파관리를 통해 개인의 삶에 대한 질적 향상에 도움을 준다.

• Genes and Genomics
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• 제40권12호
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• pp.1279-1285
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• 2018

Interdigitating dendritic cell sarcoma (IDCS) is an aggressive neoplasm and is an extremely rare disease, with a challenging diagnosis. Etiology of IDCS is also unknown and most studies with only case reports. In our case, immunohistochemistry showed that the tumor cells were positive for S100, CD45, and CD68, but negative for CD1a and CD21. This study aimed to investigate the causative factors of IDCS by sequencing the protein-coding regions of IDCS. We performed whole-exome sequencing with genomic DNA from blood and sarcoma tissue of the IDCS patient using the Illumina Hiseq 2500 platform. After that, we conducted Sanger sequencing for validation of sarcoma-specific variants and gene ontology analysis using DAVID bioinformatics resources. Through comparing sequencing data of sarcoma with normal blood, we obtained 15 nonsynonymous single nucleotide polymorphisms (SNPs) as sarcoma-specific variants. Although the 15 SNPs were not validated by Sanger sequencing due to tumor heterogeneity and low sensitivity of Sanger sequencing, we examined the function of the genes in which each SNP is located. Based on previous studies and gene ontology database, we found that POLQ encoding DNA polymerase theta enzyme and FNIP1 encoding tumor suppressor folliculin-interacting protein might have contributed to the IDCS. Our study provides potential causative genetic factors of IDCS and plays a role in advancing the understanding of IDCS pathogenesis.

• Structural Engineering and Mechanics
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• 제69권5호
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• pp.537-545
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• 2019

Stress analysis of bottom-hole rock has to be considered with much care to further understand rock fragmentation mechanism and high penetration rate. This original study establishes a fully coupled simulation model and explores the effects of overburden pressure, horizontal in-situ stresses, drilling mud pressure, pore pressure and temperature on the stress distribution in bottom-hole rock. The research finds that in air drilling, as the well depth increases, the more easily the bottom-hole rock is to be broken. Moreover, the mud pressure has a great effect on the bottom-hole rock. The bigger the mud pressure is, the more difficult to break the bottom-hole rock is. Furthermore, the maximum principal stress of the bottom-hole increases as the mud pressure, well depth and temperature difference increase. The bottom-hole rock can be divided into three main regions according to the stress state, namely a) three directions tensile area, b) two directions compression areas and c) three directions compression area, which are classified as a) easy, b) normal and c) hard, respectively, for the corresponding fragmentation degree of difficulty. The main contribution of this paper is that it presents for the first time a thorough study of the effect of related factors, including stress distribution and temperature, on the bottom-hole rock fracture rather than the well wall, using a thermo-poroelastoplasticity model.

• Computers and Concrete
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• 제27권3호
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• pp.199-210
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• 2021

The aim of this paper was to examine the continuous and discontinuous contact problems between the functionally graded (FG) layer pressed with a uniformly distributed load and homogeneous half plane using an analytical method and FEM. The FG layer is made of non-homogeneous material with an isotropic stress-strain law with exponentially varying properties. It is assumed that the contact at the FG layer-half plane interface is frictionless, and only the normal tractions can be transmitted along the contacted regions. The body force of the FG layer is considered in the study. The FG layer was positioned on the homogeneous half plane without any bonds. Thus, if the external load was smaller than a certain critical value, the contact between the FG layer and half plane would be continuous. However, when the external load exceeded the critical value, there was a separation between the FG layer and half plane on the finite region, as discontinuous contact. Therefore, there have been some steps taken in this study. Firstly, an analytical solution for continuous and discontinuous contact cases of the problem has been realized using the theory of elasticity and Fourier integral transform techniques. Then, the problem modeled and two-dimensional analysis was carried out by using ANSYS package program based on FEM. Numerical results for initial separation distance and contact stress distributions between the FG layer and homogeneous half plane for continuous contact case; the start and end points of separation and contact stress distributions between the FG layer and homogeneous half plane for discontinuous contact case were provided for various dimensionless quantities including material inhomogeneity, distributed load width, the shear module ratio and load factor for both methods. The results obtained using FEM were compared with the results found using analytical formulation. It was found that the results obtained from analytical formulation were in perfect agreement with the FEM study.

• 한국정보통신학회논문지
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• 제25권4호
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• pp.515-522
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• 2021

본 논문에서는 관형 철탑의 용접부 결함을 상시적으로 감시하기 위하여 초음파 탐상 신호에 대한 기계학습 알고리즘의 적용 방법을 제시하고 분석하였다. 기계학습 방법으로는 유전자 알고리즘에 의한 특징 선택과 서포트 벡터머신을 이용한 탐상 신호 분류 방법을 사용하였다. 특징 선택에서는 30개의 후보 특징들 가운데 피크, 히스토그램 하한 경계, 정규 음로그우도가 선택되었으며, 이들은 결함의 깊이에 따른 신호의 차이를 명확하게 나타내었다. 또한, 선택된 특징들을 서포트 벡터 머신에 적용한 결과 정상 부위와 결함 부위를 완벽하게 분류할 수 있는 것으로 나타났다. 따라서 본 연구의 결과는 향후 초음파 신호 기반 결함 성장 조기 감지시스템의 개발과 이를 통한 에너지 송전 관련 산업에 유용하게 사용될 수 있을 것으로 기대된다.