• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.109-109
• /
• 2003

Even though success in birth of live offspring from nuclear transfer(NT) using somatic cells in many species, detailed information on processes or mechanisms of development are not well known. Cytoplasm of bovine oocyte has been known to support the development of nuclear transferred embryos using nuclear donor cells from different species. Therefore, interspecies NT might be used to find answers of some questions in basic aspect of nuclear transfer In this study, we examined the developmental potential of reconstructed embryos when bovine oocyte as a cytoplasm recipient and mouse embryonic fibroblast as a nuclear donor were used. The nuclear transfer units were aliocated in Group 1 (murine block media and normal media) and Group 2. (bovine block media and normal media). NT units were not blocked at 2-cell stage regardless of types of medium. On mouse media, poor development of interspecies NT units was observed compared to bovine media. However, as NT units cultured in bovine normal medium, embryos developed over 8-cell stage. Further studies performed to increase the developmental rate in condition of antioxidant treatment. Despite low development, bovine-murine interspecies nuclear transferred embryos could develop to blastocysts and they showed that blastocyts rate of antioxidant group was superior to those of non-antioxidant group. Next, we investigated gene expression pattern which is carried out for zygotic activation. The Xist gene is expressed in female mouse embryo after zygotic activation of 4-cell stage. But interspecies nuclear transferred embryos do not express Xist gene at 4-cell stage. As a result, it is suggested that the bovine cytoplasm controls the early preimplantation development in interspecies NT However, the development of later stages might require genomic control from transferred donor nucleus. Therefore, even though the involvement of several other factors such as mitochondrial incompatibility, effective development of embryos produced by interspecies NT requires proper genomic activation of donor nucleus after overcoming the cytoplasmic control of recipient oocytes.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.7
• /
• pp.937-942
• /
• 2009

The aim of this study was to analyze the effects on growth and muscle development during the growing period of the sex-linked dwarf gene in the background of a Taiwan Country chicken strain, L2, selected for egg production. Eight crossbred males, heterozygous for the DW*DW mutation, were each backcrossed to six females of the L2 strain to produce two genotypes of BC females, either normal (DW*N+/-) or dwarf (DW*DW/-). The experiment included 251 normal and 207 dwarf pullets. The effect of the dwarf gene on body weight and shank length was highly significant from 2 weeks of age. The reduction of body weight by the dwarf gene reached 34.8% and 37.4% as compared to normal sibs at 16 and 20 weeks of age, respectively. Parameters of the growth curve were estimated: the age at inflection (TI) was higher in normal pullets (66.9 days) than in dwarf pullets (61.2 days). A significant effect of the dwarf gene on single muscle fiber cross-section area was found from 12 weeks of age onwards, whereas the dwarf gene had no effect on the total number of muscle fibers. Comparing the effect of the dwarf gene on shank length at different ages revealed an earlier effect on skeleton growth, observed from 2 weeks of age, than on muscle development, which was affected from 8 to 12 weeks of age.

• Proceedings of the Korea Concrete Institute Conference
• /
• 1996.04a
• /
• pp.192-197
• /
• 1996

An experimental study was performed to examine the feasibility of using silicic wastes as construction materials for civil structures, and investigate its utility as a replacement for the favored nature resource to prevent the economic loss. In order to achieve this objective, mechnical properties of concrete containing silicic wastes is tested by investigating the strength development through parameters of water-binder ratios replacement 10 percent ratio with respect to curting conditions. The effect of stringth development is investigated for curing conditions when silicic wastes of 10 percent of cement-binder ratios is containde. Comparision on compressive strength of normal concrete and concrete containing silicic wastes at 28 day is conducted. The concrete with silicic wastes have larger compressive strength than of normal concrete by about 20 percent, when cured at 80 degree. The wastes concrete using silica sand shows increased strength, fracture toughness, elastic modulus and strain than the normal concrete, although the silicic wastes concrete could be able to satisfy the generally required strength for conventional concrete structures.

• Korean Journal of Animal Reproduction
• /
• v.9 no.1
• /
• pp.36-39
• /
• 1985

This experiment was carried out to investigate the morphology of bovine embryos thawed after deep freezing at -196$^{\circ}C$ and the development of frozen-thawed embryos after in vitro culture in Ham's F-10 medium with 10% NBCS. The results obtained were summarized as follows: 1. The propotion of embryos which a, pp.ared mophologically normal was averaged 77.5% (79/102). 2. The morphologically normal rate of frozen-thawed blastocyst (78.6%) was higher than that of morula (76.7%), but there was no significant difference. 3. Normal development was observed in 20 of 68 embryos cultured for 24-72hr in medium and overall survival rate was 29.4%. 4. Survival rate fo blastocyst (33.3%) was higher than that of morula (25.7%).

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 1998.03a
• /
• pp.67-70
• /
• 1998

There were many cases to apply artificial intelligence to medicine. In this paper, we present the prediction model of the development of the NIDDM(noninsulin-dependent diabetes mellitus). It is not difficult that doctor diagnose patient as DM(diabetes mellitus). However NIDDM is usually developmented later on 40 years old and symptom appeares gradually. So screening test or prediction model is needed absolutely. Our model predicts development of NIDDM with still normal data 2 year ago. Prediction models developed are both MLP(multilayer perceptron) with backpropagation training and RBFN(radial basis function network). Performance of both models were evaluated with likelihood ratio. MLP was about two and RBFN was about three. We expect that models developed can prevent development of DM and utilize normal data.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.7 no.7
• /
• pp.229-241
• /
• 1999

The paper describes the development of the ABS(Anti-Lock Bracke Sytem) real-time simulator which is composed of the real hydraulic modulator, the brake system, and the control software. This useful too supports the development enviornment of the ABS in great flexible mamer. It offers an efficient and cost-effective method of ABS development which includes the various realistic road conditons, the vehicle characteristics , and the brake characteristics. The performance of the ABS is compared with the normal braking results. Thepresented experimental results are braking on the high friction road, thetransient friction road(high to low , low to high), the split friction road, and the high friction road with steer angle. The paper shows the effectiveness and the safety of the ABS compared with the normal brake system , and the powerful and conventient tool in developing the ABS.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2004.11a
• /
• pp.765-768
• /
• 2004

This study reports the site application of the early strength development technology for form stripping earlier than normal state in the apartment construction. It is a method of framework rationalization and it needs the technology of early strength development concrete and 6-day cycle process. We applied the method to the apartment construction to reduce the floor construction period and to maximize the economical effect by reduction of the period of total construction. As a result, we confirmed the superior property of the early strength development of the concrete and reliable success of 6-day cycle process.

• The korean journal of orthodontics
• /
• v.4 no.1
• /
• pp.49-55
• /
• 1974

The author studied the open-bite of 16 males and 25 females aged 20-23 using profile cephalometric roentgenogram and compared with normal occlusion to determine the morphological pattern of open-bite. The following results are obtained. 1) Open-bite patients showed remarkable vertical development of jaws than normal occlusion. 2) Females indicated conspicuous characteristics than males in open-bite. 3) Anterior teeth disclosed low positions in open-bite patients. 4) Mandible revealed obvious morphological features than maxilla in open-bite patients.

• Proceedings of the Korea Environmental Mutagen Society Conference
• /
• 2002.05a
• /
• pp.14-23
• /
• 2002

Development of oligodendrocytes and the generation of myelin internodes within the spinal cord depends on regional signals derived from the notochord and axonally derived signals. Neuregulin (NRG)-1, localized in the floor plate as well as in motor and sensory neurons, is necessary for normal oligodendrocyte development. Oligodendrocytes respond to NRGs by activating members of the erbB receptor tyrosine kinase family. Here, we show that erbB2 is not necessary for the early stages of oligodendrocyte precursor development, but is essential for proligodendroblasts to differentiate into galactosylcerebroside-positive (GalC+) oligodendrocytes. In the presence of erbB2, oligodendrocyte development is normal. In the absence of erbB2 (erbB2-/-), however, oligodendrocyte development is halted at the proligodendroblast stage with a >10-fold reduction in the number of GalC+ oligodendrocytes. ErbB2 appears to function in the transition of proligodendroblast to oligodendrocyte by transducing a terminal differentiation signal, since there is no evidence of increased oligodendrocyte death in the absence of erbB2. Furthermore, known survival signals for oligodendrocytes increase oligodendrocyte numbers in the presence of erbB2, but fail to do so in the absence of erbB2. Of the erbB2-/- oligodendrocytes that do differentiate, all fail to ensheath neurites. These data suggest that erbB2 is required for the terminal differentiation of oligodendrocytes and for development of myelin.

• Communications for Statistical Applications and Methods
• /
• v.7 no.1
• /
• pp.285-290
• /
• 2000

We noted a property of a stationary distribution on the matrix C, which is the covariance matrix of order statistics of standard normal distribution That is the sup norm of th powers of C is ee' divided by its dimension. The matrix C can be taken as a transition probability matrix in an acyclic Markov chain.