• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1551-1558
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• 2014

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.730-738
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• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.

• Journal of Environmental Health Sciences
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• v.35 no.6
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• pp.486-490
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• 2009

In January 2009, the water quality of offshore around the Incheon coast was evaluated by bioassay using early development systems of a sea urchin species, Hemicentrotus pulcherrimus. The results of performing biological evaluations on seawater samples from total of thirteen sites, showed that the formation rates of normal pluteus larva varied from 18% to 71%. In site 5 the seawater sample led to an averaage formation rate of normal larva of 18%, the highest abnormal formation rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 71%, the highest formation rate of normal larva. Seawater samples from site 1, 2, 4, 7, 9, 10, 11 and 12, resulted in average formation rates of normal larva from 33% to 56%, which indicates the developmental damage of early embryos is not severe. Seawater samples from site 5, 6, 8 and 13, resulted in average formation rates of normal larva from 18% to 21% which there was strong damage to the development of early embryos.

• Korean Journal of Environmental Biology
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• v.22 no.3
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• pp.376-380
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• 2004

In August 2003, the water quality of offshore waters along the Incheon coast of Korea was evaluated by biological evaluation using gametes, embryos and early development systems of a starfish species (Asterina pectinifera). As the result of performing biological evaluations on seawater samples from a total thirteen sites, the formation rate of normal larva was 16-68%. At seawater sample from site 5 and 13, formation rate of normal larva averaged 16%, the most abnormal rate hindering the early embryo development of the experimental animal, while that of site 3 averaged 68%, the highest formation rate of normal larva. At seawater sample from site 2, 4, 7, 9, 10, 11, 12, formation rate of normal larva averaged 33-54%, those which damage the development of early embryos slightly. At seawater sample from site 1, 5, 6, 8, 13, formation rate of normal larva averaged 16-28%, those which damage the development of early embryos strongly.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.1
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• pp.217-224
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• 2021

This research seeks to determine what intellectual capital represented by indicators of conceptual skills, human skills, and technical skills plays a role in the development of financial technology. The consideration of fintech is more practical and economical. The concept of fintech is related to the rapid development of global technology by creating various new technologies, especially computer technology. This research uses secondary data; the population of this study is the top management companies in Indonesia during the new normal era. The sample in this research used a purposive sampling method, and the quantitative method. The results of this research indicate that the intellectual capital variable represented by conceptual skills has a significant positive role in the development of financial technology in the new normal era. This research posits that intellectual capital also has a role in the development of financial technology in the new normal. This is because the new normal period represents currently a new challenge in responding to the economic crisis that is resulting from Covid-19 pandemic around the world. Therefore, new concepts, new humanity, and new techniques are needed to develop financial technology, so that they can exist and encourage economic growth in this Covid-19 pandemic era.

• Journal of Korean Physical Therapy Science
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• v.3 no.1
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• pp.835-841
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• 1996

The purpose of this study is to investigate the relationship between the development of laterality and bilateral motor abilities measured with respect to various rhythm movements in upper extremity of mentally retared children. The development of laterality was measured by a survey of dominace-hand. The bilateral motor abilities were assessed by means of a tentative rhythm beat test of hands. One hundred sixty eighty subjects with special education needs shose age ranged from 7 to 18 years old were used for an experimental group. Fity nine normal children whose age ranged from 3 to 6 years old were also chosen as a control group. The major findings were a follows: 1. The development of lateral dominace-hand in mentally retared children differed from that of normal subjects with as increase in chronological age; the degree of the attainment of lateral dominance - hand was 75 percest of the mean level of the normal children. Though normal children establish a dominance - hand by ths age of years old, meatally retarded children are likely to show 57 percent of normal development at the age of 7 through 9 years old and 65 percent of normal development at the age of 16 through 18 years old. 2. A greater delay in mentally retarded was found through a rhythm test which was development to assess bilateral motor abilities in upper extremity. A closser relationship betwin chronological age and the development of bilateral motor abilities in mentally retared children was found. 3. In view of these findings, it was indicated that bilateral motor abilities of the retarded and non-retarded children were significantly correlated to the acqusition of dominance-hand.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1865-1875
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• 2018

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, ${\mu}$ of mycelium reached to $0.15h^{-1}$ after 45 h of culture in a thermostatic water bath incubator at $30^{\circ}C$. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

• Speech Sciences
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• v.9 no.3
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• pp.121-131
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• 2002

The purpose of the present study was to investigate the characteristics of receptive and expressive language development of cleft palate infants aged under 3. Twenty-six cleft palate infants and 52 normal infants were grouped into 3 chronological age groups with 1 year intervals: less than 1, 1, and 2 years old. The cleft palate infants were divided into 2 groups: cleft palate only, and cleft lip & palate. Each mother of the infants was asked to complete the questionnaire, Sequenced Language Scale for Infants (SELSI). Receptive and Expressive language scores of SELSI were computed. The scores of receptive and expressive language were respectively analyzed into 4 categories of language: phonology/prosody, semantics, syntax, and pragmatics. The results, concerning the differences of language development between the cleft palate and the normal infants, were as follows: (1) expressive language scores were significantly different at age 2 between the cleft palate and the normal infants; (2) cleft plate groups aged less than 1 and 1 showed lower scores of phonology/prosody of expressive language than the normal groups; (3) cleft palate group aged 2 showed lower scores than the normal group in semantics, syntax, and pragmatics. The results, concerning the characteristics of language development between the cleft palate only and the cleft lip & palate infants, were as follows: (1) the scores of expressive and receptive language were not statistically different between the 2 groups; (2) both groups did not show any difference in .the scores of phonology/ prosody, semantics, syntax, and pragmatics of receptive and expressive language.

• Educational Technology International
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• v.6 no.2
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• pp.1-9
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• 2005

Starting with analyzing the development of cyber-education in China, this article discusses experiences and lessons acquired through making reflections on the following aspects: technology vs. pedagogy, the degree education vs. non-degree education, resource exclusivism vs. resource inclusivism, mono-service system vs. multi-service system, and educational quality vs. economic profit. Finally, the paper puts forward some suggestions on the future development of cyber-education in China.

• The Journal of Korean Physical Therapy
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• v.9 no.1
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• pp.195-204
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• 1997

The Purpose of this study was to compare the different of normal development and walking characteristics of children with spastic cerebral palsy, and to guide theraputic approach for improve on walking. Key contributions from normal development of standing and walking, abnormol walking pattern of children with spastic cerebral palsy are studies. Spastic cerebral palsy includes an increased factors of problem in standing, walking : These are deformity of lowerextremities, arm used for balancing, asymmetic walking, abnormal walking patterns, build .up Treatment goal is to normal walking patterns. and to prevent secondary deformity. In this study, normal walking is very important factors in daily activities, then to recovery function of children with cerebral palsy, its need more adapted several method.