• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1663-1666
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• 2012

Background and Objective: Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. Materials and Methods: Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI-TOF mass spectra. Results: Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, includimng HSPB1and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings. Conclusions: Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.3
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• pp.386-392
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• 1996

A numerical method based on genetic algorithms was introduced to characterize the grain-size distribution more effectively. This technique was proved significant particularly for multimodal size distributions, as was verified for samples from Korea Strait continental shelf. Sediment samples collected from the Korea Strait continental shelf revealed that $96\%$ of the grain-size distributions were multimodal. Therefore, the use of grain-size parameters was not the ideal method. As an alternative method, the decomposition of sue curves into elementary normal component curves was used. Means and standard deviations of 593 decomposed normal components were calculated by a numerical method from 268 size curves of Korea Strait sediments. The mean values of decomposed normal components showed peaks at $1\~3\phi\;and\;7\~9\phi$ size classes. The plot of mean and standard deviation values of the coarse fraction normal components on the map showed a characteristic areal distribution. The characteristic distribution was found to derive from underlying Pleistocene sediment on the basis of sea bottom geologic distribution of the area. The method of decomposition into normal components was found to be more effective than the analysis using traditional grain-size parameters in investigation of multimodal size distribution of Korea Strait shelf sediment.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.5 no.2
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• pp.9-16
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• 2010

Objectives : This study is planned to classify different biomechanics of men and women, from the comparison of normal range and hip internal rotation angle in chronic low back pain patients. Methods : We measured the hip internal rotation angle of the 30 men and 30 women patients with low back pain that has been over 3 months in BuCheon Jaseng Hospital of Oriental medicine. We set 35-45 degrees as normal range, and found the patients with exceeding normal range, the patients with normal range, and the patients with under normal range. Results : Men appeared to have less hip internal rotation angle than women. Especially, the men's left hip internal rotation angle was less than normal range. On the other hand, women's right hip internal rotation angle was often larger than normal range which was stastically significant. Conclusions : Contraction of hip external rotation muscle including gluteus maxius muscle and piriformis muscle in men, or relaxation of posterior gluteus medius, gluteus maxius and piriformis muscle with contraction of tensor fasciae latae, a part of hip internal rotation muscle, in women seems to be the basis for biomechanics of chronic low back pain.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.5
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• pp.2393-2399
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• 2013

In this paper, we proposed a method to screening of Alzheimer's disease (AD) from Raman spectra of platelet with synthesis of basis spectra using singular value decomposition (SVD). Raman spectra of platelet from AD transgenic mice are preprocessed with denoising, removal background and normalization method. The column vectors of each data matrix consist of Raman spectrum of AD and normal (NR). The matrix is factorized using SVD algorithm and then the basis spectra of AD and NR are determined by 12 column vectors of each matrix. The classification process is completed by select the class that minimized the root-mean-square error between the validation spectrum and the linear synthesized spectrum of the basis spectra. According to the experiments involving 278 Raman spectra, the proposed method gave about 97.6% classification rate, which is better performance about 6.1% than multi-layer perceptron (MLP) with extracted features using principle components analysis (PCA). The results show that the basis spectra using SVD is well suited for the diagnosis of AD by Raman spectra from platelet.

• The Journal of Korean Academy of Prosthodontics
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• v.26 no.1
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• pp.171-184
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• 1988

The establishment of an optimal and functional condylar position (centric relation) as the therapeutic and diagnostic reference position during occlusal treatment for patients with temporomandibular joint and muscle pain. dysfunction has long been an important subject in dentistry. The objective of this study was to compare the reproducibility md the changes of condylar position in normal group to those in patient group after use of the occlusal biteplane splint. For this study, 11 normal adults who had no symptoms of masticatory dysfunction and 18 patients who had visited at the department of occlusion in dental infirmary of Yonsei University were selected. For each subject three centric relation records were recorded before treatment, after 2 weeks and after symptoms were improved. And the condylar positions in centric relation were measured using articulators and a Vericheck. On the basis of this study, the following results were obtained. 1. In normal group, there was no significant difference of reproducibility in condylar position before and after the use of the occlusal biteplane splint for 2 weeks. 2. In patient group there was significant improvement in the reproducibility of condylar position after treatment (P<0.01). The reproducibility in patient group, however, was less than normal group. (P<0.01) 3. The mean distance of condylar movements was $0.38{\pm}0.22mm$ after 2 weeks in normal group. 4. In patient group, condylar movements were $1.36{\pm}0.70mm$ (P<0.01), significantly different from normal group. (P<0.01) 5. In patient group, the main direction of condylar movements after treatment was toward anterior and superior, preponderance being at superior direction.

• Parasites, Hosts and Diseases
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• v.51 no.3
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• pp.327-334
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• 2013

Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an ${\alpha}$-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks.

• 한국해양학회지
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• v.28 no.2
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• pp.114-120
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• 1993

Numerical method of nonlinear regression was introduced to characterize grain-size distribution more effectively than using the traditional textural parameters. This technique proved critical particularly to multimodal size distributions, as exemplified by samples from Cheju strait continental shelf. Grain-size analysis of samples collected from the Cheju Strait continental shelf reveals that 86% of the grain-size distributions are multimodal. As multimodal grain-size distribution deviates from the statistical (log) normal distribution, the grain-size parameters traditionally used in sediment studies do not describe the distribution efficiently. Therefore, the use of grain-size curves into elementary normal component curves was used. Means and standard deviations of 387 decomposed normal components were decided by a decomposition method (nonlinear least square regression) from 167 size curves of the Cheju Strait sediments. The mean values of decomposed normal components show peaks at 1-3 phi and 8-9 phi size classes. The plot of mean values of the coarse fraction normal components on the map shows a characteristic and complex areal distribution. On the basis of the areal distribution of the mean values of the components and that of isopach of total Plenipotence sediment, the areal distribution of layers composing a transgressive sand of Late Plenipotence age were revealed.

• Toxicological Research
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• v.19 no.3
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• pp.247-257
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• 2003

Medicine is undergoing a revolution in the understanding of the mechanisms through which disease processes develop. The advent of genetics and molecular biology to oncology not only is providing surrogate predictors of therapy response and survival which are forming the basis for selection among established treatment options, but is providing targets for new directions in therapy as well. Molecular modification of somatic cells for the purposes of protecting the normal cells from the toxicity of cancer chemotherapy, for the sensitization of the tumor cells to therapy and use of conditionally replicating viral vector have been new directions of cancer treatment which have reached the clinical arena. Advances in molecular pharmacology and vector design summarized in this paper may provide solutions to some of the existing problems in the technology of gene transfer therapy. Continued basic research into the biological basis of human disease, systemic studies of the application of these discoveries to therapy and the improvement of vector for gene delivery all combined may result in advances in this important field of therapy over the next few years.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.3 s.168
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• pp.107-114
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• 2005

A new approach which combines implicit surface scheme and point projection method is presented in order to fill the arbitrarily shaped holes in the polygon model. In the method a trimmed surface which has an outer boundary curve is generated by using the implicit surface scheme and normal projection of point onto the base surface. The base surface is constructed by creating smooth implicit surface from the incomplete polygon model through which the surface should pass. In this paper an implicit surface is defined by a radial basis function, a continuous scalar-valued function over the domain $R^3$. The base surface is the set of all points at which this scalar function takes on the value zero and is created by placing zero-valued constraints at the vertices of the polygon model. In order to show the validity of the present study, various hole fillings are carried out for the complex polygon model of arbitrary topology.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.04a
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• pp.335-342
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• 1999

It is well known that l-girders are weak in torsion and it might be more economical to use a box girder, which has great torsional rigidity. The use of box beams does, however, present a potential problem in that cross-sectional distortions can induce large warping normal stresses and transverse bending stress. Accordingly a sufficient number of diaphragms are provided to make the distortional effects minimal. In engineering practice, diaphragms are spaced in 5m intervals without reasonable basis. It is considered to be noneconomical design to the almost design engineers, and it may produce the unsafe structural systems in special cases such as curved bridges with large initial curvature. These problems have not been solved for the lack of adequate tools of structural analysis. In this study, on the basis of the parametric studies, the design formulas for the distortional warping stress and the reasonable diaphragm spacing of box girder were presented.