Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Comparative Proteomics Analysis of Colorectal Cancer

  • Wang, Jun-Jiang (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Liu, Ying (Department of Obstetrics and Gynecology, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Zheng, Yang (Department of Gastroenterology Surgery, the First Affiliated Hospital of Shan-tou University Medical College) ;
  • Lin, Feng (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Cai, Guan-Fu (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Yao, Xue-Qing (Department of Gastrointestinal Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital)
  • Published : 2012.04.30
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Abstract

Background and Objective: Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. Materials and Methods: Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI-TOF mass spectra. Results: Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, includimng HSPB1and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings. Conclusions: Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

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