• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 추계학술대회 논문집 전기물성,응용부문
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• pp.274-276
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• 2001

This research aims to develop the multiple channel electrochemical DNA chip using microfabrication technology. At first, we fabricated a high integration type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized and reacted. Cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. Therefore, it is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• 한국음향학회지
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• 제32권1호
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• pp.79-85
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• 2013

CASA 시스템의 특징 추출은 시간의 연속성과 채널 간 유사성을 이용하여 청각 요소의 상관지도를 구성하여 사용한다. 채널 간 유사성을 교차 상관 계수를 이용하여 특징 추출 할 경우 상관성을 정량적으로 나타내기 위해 계산량이 많은 단점이 있다. 따라서 본 논문에서는 특징 추출 시 계산 량을 줄이기 위한 방법으로 비모수적 상관 계수를 이용한 특징 추출 방법을 제안하고 이를 CASA 시스템을 통하여 목적 음성을 분리하는 실험을 수행하였다. 목적 음성의 분리 성능을 평가하기 위하여 신호 대 잡음비를 측정한 결과, 제안 방식이 기존 방식에 비해 평균 0.14 dB의 미세한 성능 개선을 보였다.

• Genomics & Informatics
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• 제12권3호
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• pp.80-86
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• 2014

Foldback intercoil (FBI) DNA is formed by the folding back at one point of a non-helical parallel track of double-stranded DNA at as sharp as $180^{\circ}$ and the intertwining of two double helixes within each other's major groove to form an intercoil with a diameter of 2.2 nm. FBI DNA has been suggested to mediate intra-molecular homologous recombination of a deletion and inversion. Inter-molecular homologous recombination, known as site-specific insertion, on the other hand, is mediated by the direct perpendicular approach of the FBI DNA tip, as the attP site, onto the target DNA, as the attB site. Transposition of DNA transposons involves the pairing of terminal inverted repeats and 5-7-bp tandem target duplication. FBI DNA configuration effectively explains simple as well as replicative transposition, along with the involvement of an enhancer element. The majority of diverse retrotransposable elements that employ a target site duplication mechanism is also suggested to follow the FBI DNA-mediated perpendicular insertion of the paired intercoil ends by non-homologous end-joining, together with gap filling. A genome-wide perspective of transposable elements in light of FBI DNA is discussed.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.234-247
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• 2021

We used a heterozygous gene deletion library of fission yeasts comprising all essential and non-essential genes for a microarray screening of target genes of the antifungal terbinafine, which inhibits ergosterol synthesis via the Erg1 enzyme. We identified 14 heterozygous strains corresponding to 10 non-essential [7 ribosomal-protein (RP) coding genes, spt7, spt20, and elp2] and 4 essential genes (tif302, rpl2501, rpl31, and erg1). Expectedly, their erg1 mRNA and protein levels had decreased compared to the control strain SP286. When we studied the action mechanism of the non-essential target genes using cognate haploid deletion strains, knockout of SAGA-subunit genes caused a down-regulation in erg1 transcription compared to the control strain ED668. However, knockout of RP genes conferred no susceptibility to ergosterol-targeting antifungals. Surprisingly, the RP genes participated in the erg1 transcription as components of repressor complexes as observed in a comparison analysis of the experimental ratio of erg1 mRNA. To understand the action mechanism of the interaction between the drug and the novel essential target genes, we performed isobologram assays with terbinafine and econazole (or cycloheximide). Terbinafine susceptibility of the tif302 heterozygous strain was attributed to both decreased erg1 mRNA levels and inhibition of translation. Moreover, Tif302 was required for efficacy of both terbinafine and cycloheximide. Based on a molecular modeling analysis, terbinafine could directly bind to Tif302 in yeasts, suggesting Tif302 as a potential off-target of terbinafine. In conclusion, this genome-wide screening system can be harnessed for the identification and characterization of target genes under any condition of interest.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제55권9호
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• pp.443-448
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• 2006

Non-contacting capacitive sensor based on Thompson-Lampard theorem have been fabricated and characterized for measuring of 때 order displacements. To overcome disadvantages of the existed capacitive sensors of parallel plate type with 2-electrodes and 3-electrodes, the developed new sensor was designed to have 4-electrodes with a constant gap of 0.2mm between the electrodes. Two of the electrodes were used as a high potential electrode and a low one, the other two electrodes were used as guard electrodes. These electrodes were made from copper using RF sputtering system on a sapphire plate with diameter 17 mm and thickness 0.7 mm. This sensor can be used for measuring the distance not only between the sensor and metallic target connected to ground potential but also non-metallic target without ground connection.

• 음성과학
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• 제9권4호
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• pp.15-25
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• 2002

It's not easy to produce very high tones during singing for not only untrained ordinary people but also even trained singers. To get high singing tones from the low tones, some trained singers used to use a distinguished singing technique, Passaggio (vocal register transition). The purpose of this study is to compare several voice parameters variation between when to sing with using the passaggio technique and to sing without using it. We selected 18 male singers (tenor 8, baritone 10), who had more than 7 years of experience and were well trained in passaggio technique. Simultaneous measurements of fundamental frequency (F0), mean flow rate (MFR), intensity (I), and subglottal pressure (Psub) were performed using the phonatory function analyzer (Nagashima). For the tenor, target tones /a/ were presented: 1) easy phonation: $B_{2}$, 2) high tone without passaggio: F$#_{3}$ 3) high tone with passaggio: F$#_{3}$. For the baritone, target tones /a/ were presented: 1) easy phonation: G$#_{3}$, 2) high tone without passaggio: D$#_{3}$, 3) high tone with passaggio: D$#_{3}$. F0 of the target tones between non-passaggio group and passaggio group was almost the same in both tenor and baritone groups. Intensity of the non-passaggio and passaggio vocalization was much louder than that of easy phonation and pasaggio was louder than non-passaggio vocalization (especially statistically significant in baritone singers). MFR of the passaggio vocalization was greater than non-passaggio vocalization in both tenor and baritone group, but statistically significant only in baritone. Psub of the passaggio vocalization was greater than that of the non-passaggio vocalization in both tenor and baritone group, but statistically not significant in tenor.

• Parasites, Hosts and Diseases
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• 제46권4호
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• pp.217-221
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• 2008

Free-living Naegleria fowleri leads to a fatal infection known as primary amebic meningoencephalitis in humans. Previously, the target cell death could be induced by phagocytic activity of N. fowleri as a contact-dependent mechanism. However, in this study we investigated the target cell death under a non-contact system using a tissue-culture insert. The human microglial cells, U87MG cells, co-cultured with N. fowleri trophozoites for 30 min in a non-contact system showed morphological changes such as the cell membrane destruction and a reduction in the number. By fluorescence-activated cell sorter (FACS) analysis, U87MG cells co-cultured with N. fowleri trophozoites in a non-contact system showed a significant increase of apoptotic cells (16%) in comparison with that of the control or N. fowleri lysate. When U87MG cells were co-cultured with N. fowleri trophozoites in a non-contact system for 30 min, 2 hr, and 4 hr, the cytotoxicity of amebae against target cells was 40.5, 44.2, and 45.6%, respectively. By contrast, the cytotoxicity of non-pathogenic N. gruberi trophozoites was 10.2, 12.4, and 13.2%, respectively. These results suggest that the molecules released from N. fowleri in a contact-independent manner as well as phagocytosis in a contact-dependent manner may induce the host cell death.

• 한국안광학회지
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• 제17권4호
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• pp.435-440
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• 2012

목적: 본 연구는 $2{\times}$ 배율을 가지는 도트사이트 장치의 개발에 관한 것이다. 방법: 무배율 도트사이트로 먼 거리를 주시할 때 목표물을 확대해서 볼 수 있도록 하기 위해 기존 무배율 도트사이트에 착탈식으로 장착할 수 있는 새로운 광학계를 개발한다. 이는 기존 무배율($1{\times}$) 도트사이트를 $2{\times}$ 배율 도트사이트로의 전환을 필요에 따라 쉽게 할 수 있도록 하기위한 것이다. 결과: 배율이 없는 도트사이트에 착탈할 수 있는, doublet 렌즈가 쌍으로 구성되는, $2{\times}$ 배율 어포칼 광학계를 설계 제작하였다. 이를 사용하였을 때 우리는 외부 목표점을 2배 확대하여 조준할 수 있어서 조준의 정확성을 더욱 높일 수 있었다. 결론: 배율이 없는 일반 도트사이트에 착탈할 수 있는 $2{\times}$ 배율 어포칼 광학계를 설계 제작할 수 있었다. 이것에 의해 외부 목표점을 2배 확대하여 조준할 수 있도록 함으로서 조준의 정확성을 더욱 높일 수 있게 되었다.

• 한국정보통신학회논문지
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• 제16권10호
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• pp.2137-2142
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• 2012

수중환경 하에서 표적을 탐지하고 식별하는 문제는 군사적인 목적은 물론 비군사적 목적으로도 많은 연구가 수행되어 왔다. 수중환경에서의 수중음향 신호가 시간 공간적으로 특성이 변화하며 천해 다중경로 환경을 반영하는 복잡한 특성을 보이는 점으로 인해 능동 표적인식 기술은 매우 어려운 기술로 여겨져 왔다. 또한 실제 데이터 수집의 어려움이 따르게 된다. 본 논문에서는 수중환경 하에서 능동 표적신호를 합성, 특징추출 및 표적식별을 수행할 수 있는 시뮬레이터를 구현하였다. 표적신호의 합성에는 하이라이트 모델과 3차원 모델을 사용하였으며, 표적신호의 식별을 위해서는 다중각도에 기반한 은닉 마코프모델을 사용하였다.

• 한국신뢰성학회:학술대회논문집
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• 한국신뢰성학회 2001년도 정기학술대회
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• pp.311-319
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• 2001

All indices that are now in use assume normally distributed data, and any use of the indices on non-normal data results in inaccurate capability measurements. Therefore, $C_{s}$ is proposed which extends the most useful index to date, the Pearn-Kotz-Johnson $C_{pmk}$, by not only taking into account that the process mean may not lie midway between the specification limits and incorporating a penalty when the mean deviates from its target, but also incorporating a penalty for skewness. Therefore we propose, a new process capability index $C_{psk}$( WV) applying the weighted variance control charting method for non-normally distributed. The main idea of the weighted variance method(WVM) is to divide a skewed or asymmetric distribution into two normal distribution from its mean to create two new distributions which have the same mean but different standard distributions. In this paper we propose an example, a distribution generated from the Johnson family of distributions, to demonstrate how the weighted variance-based process capability indices perform in comparison with another two non-normal methods, namely the Clements and the Wright methods. This example shows that the weighted valiance-based indices are more consistent than the other two methods In terms of sensitivity to departure to the process mean/median from the target value for non-normal process.s.s.s.