• Tuberculosis and Respiratory Diseases
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• 제56권4호
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• pp.393-404
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• 2004

연구배경 : 유전자 재결합 반응에 있어서 다른 종류의 RNA의 첨가에도 불구하고 유전자 반응에 영향이 없어야 여타 실험의 정량적 분석에 이용이 가능하다. 이에 저자들은 쥐를 대상으로 filter hybridization방법과 SP-A mRNA을 이용하여 비특이성 RNA 즉, 쥐의 비장 RNA의 첨가가 surfactant protein A (SP-A)의 유전자 재결합반응의 linearity, 상관계수 및 특이성에 미치는 영향을 알아보기 위하여 이 연구를 시행하였다. 방 법 : SP-A transcript mRNA의 정량, 즉 0, 0.1, 0.5, 1 및 2.5 ng에 비특성 RNA 즉 비장 RNA를 각각 0,1, 5 및 $10{\mu}g$을 첨가하여 filter hybridization 방법을 이용하여 SP-A mRNA양과 cpm과의 연관성을 비교정량측정하여 각각의 linearity, 상관계수 및 특이성의 분자생물학적 정도관리에 대한 비교 관찰을 하기 위하여 이 연구를 시행하였다. 결 과 : 1. 쥐의 spleen RNA 0, 1, 5, 10 및 $20{\mu}g$에 대한 cpm과의 표준곡선 및 상관계수는 Y=0.13X-19.35(X=cpm, Y=spleen RNA input)이고, 상관계수는 0.98이었다. 2. SP-A sense 전사체 0, 0.1, 0.5, 1.0, 2.5 및 5 ng에 대한 cpm과의 표준곡선 및 상관계수는 Y=0.00066X-0.046 (X=cpm, Y=SP-A mRNA 전사체)이고, 상관계수는 0.99이었다. 3. 쥐의 비장 RNA $1{\mu}g$을 첨가 후 SP-A sense 전사체 0, 0.1, 0.5, 1.0, 2.5 및 5 ng에 대한 cpm과의 표준곡선 및 상관계수는 Y=0.00056X-0.051(X=cpm, Y=SP-A mRNA 전사체)이고, 상관계수는 0.99이였다. 쥐의 비장 RNA $5{\mu}g$을 첨가 후 표준곡선은 Y=0.00065X-0.088 (X=cpm, Y=SP-AmRNA 전사체)이고, 상관계수는 0.99이였다. 쥐의비장 RNA $10{\mu}g$을 첨가 후 표준곡선은 Y=0.00051X-0.10 (X=cpm, Y=SP-A mRNA 전사체)이고, 상관계수는 0.99이었다. 결 론 : 이상의 결과는 비특이성 RNA인 비장 RNA의 첨가 후 SP-A sense mRNA양과 cpm과의 상관관계는 sense 유전자와 anti-sense 유전자의 유전자 재결합 반응에 있어서 다양한 양의 비특이성 RNA의 첨가나 오염에도 불구하고 linearity, 상관계수 및 그 특이성이 잘 유지됨을 입증해 준 결과라 생각된다.

• 한국응용과학기술학회지
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• 제33권3호
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• pp.540-548
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• 2016

sodium bis-(2-ethylhexyl)sulfosuccinate-water 라멜라 액정의 비뉴톤 유동곡선을 cone-plate 레오메타를 사용하여 여러 농도와 온도 조건에서 얻었다. 이러한 비뉴톤 유동곡선을 비뉴톤 유동식에 적용하여 유동파라메타를 구하였다. 특별히 주목할 점은 액정시료의 전단속도에 대한 전단응력은 증가와 감소에서 틱소트로피와 다일레턴시 현상을 보여 hysteresis loop를 나타내고 있다는 점이다. sodium bis-(2-ethylhexyl)sulfosuccinate-water 라멜라 액정은 작은 전단속도에서는 약한 젤 현상을 보이지만 응력이 한계 응력 이상에서는 비 선형 점탄성 성질을 나타낸다. 전단속도 감소에서 분산계는 전단속도가 증가할 때 측정된 값 보다는 큰 구조변화와 전단응력을 유지하고 있다.

• Annals of Occupational and Environmental Medicine
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• 제35권
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• pp.14.1-14.10
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• 2023

Background: Shift work is known to cause changes in the circadian rhythm of the human body and adversely affect not only physical health but also mental health. Some studies have demonstrated the correlation between shift work and thyroid stimulating hormone (TSH), a hormone that changes according to the diurnal rhythm, but few studies have reported the different TSH levels according to the shift work type. This study aimed to investigate changes in TSH according to the shift work type. Methods: This study included 1,318 female workers who had a medical checkup at a university hospital in Changwon from 2015 to 2019. Shift work types were classified as non-shift work, regular 2 shifts, and irregular three shifts, and a TSH ≥ 4.2 mIU/L was defined as abnormal. A general linear model (GLM) was used to compare the TSH levels and the risk of subclinical hypothyroidism in each year, and a binary logistic analysis was performed using a generalized estimation equation (GEE) to compare the risk of subclinical hypothyroidism over the 5-year period. Results: Of the 1,318 participants included in this study, 363, 711, and 244 were non-shift, two-shift, and irregular three-shift workers, respectively. In the GEE analysis, after adjusting for age, body mass index, smoking, and alcohol consumption, the odds ratios (ORs) were 1.81 (95% confidence interval [CI]: 1.15-2.86; p = 0.011) in 2 shifts and 2.02 (95% CI: 1.23-3.32; p = 0.006) in irregular three shifts, compared to non-shift. Conclusions: Our results showed that shift work had a higher risk of subclinical hypothyroidism than non-shift work and that there was a significant difference in the risk of subclinical hypothyroidism according to the shift work type. These findings suggest that the shift work type can be considered in future thyroid function tests and evaluations.

• 대한치과교정학회지
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• 제11권2호
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• pp.109-123
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• 1981

The author studied and analyzed statistically 112 adults female ranging in age from 18 to 20 years with normal occlusion, 56 adults female ranging in age from 18 to 24 years with deep overbite and 53 adults female ranging in age from 18 to 28 years with open bite by vertical cephalometric analysis. The results were as follows; 1. In comparing normal occlusion with deep overbite and open bite, skeletal linear measurements were more significant than dentoalveolar linear measurements. SN-MP angle, SN-OP angle, PP-OP angle and Xi angle (ANS-Xi-Pog) were significant in anglular measurements. 2. Upper posterior facial height (SE-PNS), upper anterior alveolar height, lower posterior alveolar height, lower posterior alveolar height/lower anterior alveolar height and SN-PP angle were non significant between deep overbite and open bite. 3 The most significant items between deep overbite and open bite were lower anterior facial height (ANS-ME) and SN-MP angle. 4. Correlation coefficients of angular measurements were higher in deep overbite, while that of linear measurement total anterior facial height (N-ME) was higher in open bite. 5. In the multiple regression equation, significant variables were total anterior facial height (N-ME), lower anterior alveolar height, upper anterior alveolar height, upper posterior alveolar height, Xi angle (ANS-Xi-Pog) and ramus height (AR-Go) in deep overbite, and total anterior facial height (N-Me), lower anterior alveolar height, ramus height (AR-Go), lower posterior alveolar height, PP-MP angle and upper posterior facial height (SE-PNS) in open bite.

• Structural Engineering and Mechanics
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• 제86권1호
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• pp.1-16
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• 2023

The free vibration of temperature-dependent functionally graded plates (FGPs) resting on a viscoelastic foundation is investigated in this paper using a newly developed simple first-order shear deformation theory (FSDT). Unlike other first order shear deformation (FSDT) theories, the proposed model contains only four variables' unknowns in which the transverse shear stress and strain follow a parabolic distribution along the plates' thickness, and they vanish at the top and bottom surfaces of the plate by considering a new shape function. For this reason, the present theory requires no shear correction factor. Linear steady-state thermal loads and power-law material properties are supposed to be graded across the plate's thickness. Uniform, linear, non-linear, and sinusoidal thermal rises are applied at the two surfaces for simply supported FGP. Hamilton's principle and Navier's approach are utilized to develop motion equations and analytical solutions. The developed theory shows progress in predicting the frequencies of temperature-dependent FGP. Numerical research is conducted to explain the effect of the power law index, temperature fields, and damping coefficient on the dynamic behavior of temperature-dependent FGPs. It can be concluded that the equation and transformation of the proposed model are as simple as the FSDT.

• 대한토목학회논문집
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• 제43권4호
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• pp.469-476
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• 2023

반복하중이나 동적하중에 대한 지반의 소성모델은 지반구조물의 비선형 수치해석에 매우 중요하다. 단일 항복면 모델은 반복하중에 대해 선형적 거동을 보이는 반면, 개발된 탄성영역이 없는 J₂-경계면 소성모델은 동일한 물성치로 효과적으로 지반의 비선형성을 모사할 수 있다. 경계면 내부 항복면의 반경을 0으로 수렴시켜 탄성영역이 사라지도록 수식화하고, 소성경화 계수과 팽창률을 이용하여 소성변형 증분을 정의하였다. 개발된 모델의 응력-변형률 증분식을 제시하고, 쌍곡선 모델에 대한 소성경화 계수를 유도하였다. 삼축압축조건과 얕은기초의 반복하중에 대한 비교해석은 개발된 모델의 안정적인 수렴성, 이론식과의 일치성, 그리고 이력경로을 보여 주었다. 또한, 수정된 쌍곡선함수에 대한 소성경화 계수를 제시하여, 1차원 등가선형모델에 부합하는 모델변수 산정법을 제안하여 지반의 다차원 거동을 모델링할 수 있도록 하였다.

• 한국해안·해양공학회논문집
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• 제33권4호
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• pp.148-159
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• 2021

해수의 유동 및 염분 변화에 대한 재현성이 검증된 3차원 수치모델을 활용하여 한강 유량에 따른 경기만 염하수로에서의 담수 영향범위를 모의하였다. 염하수로 상류의 입구를 기준으로, 표층에서 28 psu의 염분이 나타나는 지점까지의 거리를 담수가 확산되는 거리로 정의하였으며, 한강으로부터 유출되는 담수 유량을 총 10가지(200~10,000 m³/s)로 구분하여 실험을 구성하였다. 수치모델 결과를 바탕으로, 비선형 회귀분석을 수행하여 유량과 담수 확산 거리에 대한 관계식을 산정하였다. 경기만 염하수로에서의 담수 영향범위는 한강 유량이 증가함에 따라 최소 강화도 남부 해역부터 최대 영흥도 북부 해역까지 확장된다. 유량과 담수 확산 거리는 비례하는 관계로 산정되었으며, 유량이 증가함에 따라 담수 확산 거리의 증가율은 점차적으로 감소하였다. 본 연구에서 산정한 관계식을 바탕으로 특정시기의 월 평균 한강 유량을 이용하여 염하수로에서의 담수 확산 거리를 추정할 수 있다. 이를 통해, 급격한 담수유출에 의한 수질 및 생태학적인 피해와 관련된 문제에 대응 및 예측할 수 있을 것으로 기대한다.

• Structural Engineering and Mechanics
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• 제90권6호
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• pp.551-568
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• 2024

The present study deals with static and dynamic behaviors including forced vibrations of an elastic rectangular nano plate on the two-parameter foundation. Firstly, the rectangular plate is assumed to be subjected to uniformly distributed and eccentrically applied concentrated loads. The governing equations of the problem are derived by considering the dynamic response of the plate, employing a series of the Chebyshev polynomials for the displacement function and applying the Galerkin method. Then, effects of the non-essential boundary conditions of the plate, i.e., the boundary conditions related to the shearing forces, the bending moments and the corner forces, are included in the governing equation of motion to compensate for the non-satisfied boundary conditions and increase the accuracy of the Galerkin method. The approximate numerical solution is accomplished using an iterative process due to the non-linearity of the unilateral property of the two-parameter foundation. The plate under static concentrated load is investigated in detail numerically by considering a wide range of parameters of the plate and the foundation stiffnesses. Numerical treatment of the problem in the time domain is carried out by assuming a stepwise variation of the concentrated load and the linear acceleration procedure is employed in the solution of the system of governing differential equations derived from the equation of motion. Time variations of the contact region and those of the displacements of the plate are presented in the figures for various numbers of the two-parameter of the foundation, as well as the classical and nano parameters of the plate particularly focusing on the non-linearity of the problem due to the plate lift-off from the unilateral foundation. The effects of classical and nonlocal parameters and loading are investigated in detail. Definition of the separation between the plate and the two-parameter foundation is presented and applied to the given problem. The effect of the lift-off on the static and dynamic behavior of the rectangular plate is studied in detail by considering various loading conditions. The numerical study shows that the effect of nonlocal parameters on the behavior of the plate becomes significant, when nonlinearity becomes more profound, due to the lift-off of the plate. It is seen that the size effects are significant in static and dynamic analysis of nano-scaled rectangular plates and need to be included in the mechanical analyses. Furthermore, the corner displacement of the plate is affected more significantly from the lift-off, whereas it is less marked in the time variation of the middle displacement of the plate. Several numerical examples are presented to examine the sensibility of various parameters associated with nonlocal parameters of the plate and foundation. Both stiffening and softening nonlocal parameters behavior of the plate are identified in the numerical solutions which show that increasing the foundation stiffness decreases the extent of the contact region, whereas the stiffness of the shear layer increases the contact region and reduces the foundation settlement considerably.

• 한국수자원학회논문집
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• 제41권9호
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• pp.895-905
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• 2008

유역의 수문현상을 해석하기 위해서는 다양한 지형자료와 수문 시계열자료가 필요하다. 최근 들어 DEM(Digital Elevation Model)과 수자원 주제도와 같은 지형자료 뿐만 아니라 수치예보자료 및 강우레이더의 관측자료와 같은 수문 시계열자료 또한 격자 형태로 제공되고 있으며, 이를 활용한 수문분석에 대한 다양한 연구가 이루어지고 있다. 본 연구에서는 이러한 격자형 자료를 이용하여 효과적으로 단기간의 강우-유출 현상을 모의하기 위한 물리적 기반의 분포형 강우-유출 모형인 GRM(Grid based Rainfall-runoff Model)을 개발하였다. 지표면 유출과 하도 유출의 모의는 운동파 방정식을 이용하고 있으며, 침투량 산정을 위해서 Green-Ampt 모형을 이용하고 있다. 지배방정식은 유한 체적법을 이용하여 이산화 하였으며, TDMA(TriDiagonal Matrix Algorithm) 방법을 이용하여 연립방정식을 풀고, 비선형 항에 대해서는 Newton-Raphson 방법으로 반복 계산함으로써 수렴해를 도출하였다. 개발된 모형은 단순화된 가상의 유역에 대해서 적용한 결과를 $Vflo^{TM}$ 모형의 모의결과와 비교함으로써 타당성을 검토하였다. 또한 위천 유역의 적용을 통해 모형의 검증 및 실제 유역에 대한 적용성을 검토하였으며, 모의결과는 관측유량의 재현성이 높은 것으로 나타났다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2017년도 춘계공동학술대회
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• pp.156-156
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• 2017

Most crop damages have been occurred by vermin(e.g., wild birds and herbivores) during the period between seeding and the cotyledon level. In this study, to minimize the damage by vermin and acquire the benefits such as protection against weeds and maintenance of water content in soil, immediately vinyl mulching after seeding was devised. Vinyl mulching has been generally covered with black color vinyl, that crop seeding locations cannot be detected by visible light range. Before punching vinyl, non-contact and non-destructive methods that can continuously determine the locations are necessary. In this study, a crop position detection method was studied that uses infrared thermal image sensor to determine the cotyledon position under vinyl mulch. The moving system for acquiring image arrays has been developed for continuously detecting crop locations under plastic mulching on the field. A sliding mechanical device was developed to move the sensor, which were arranged in the form of a linear array, perpendicular to the array using a micro-controller integrated with a stepping motor. The experiments were conducted while moving 4.00 cm/s speed of the IR sensor by the rotational speed of the stepping motor based on a digital pulse width modulation signal from the micro-controller. The acquired images were calibrated with the spatial image correlation. The collected data were processed using moving averaging on interpolation to determine the frame where the variance was the smallest in resolution units of 1.02 cm. For this study, the spline method was relatively faster than the other polynomial interpolation methods, because it has a lower maximum order of formulation when using a system such as the tridiagonal linear equation system which provided the capability of real-time processing. The temperature distribution corresponding to the distance between the crops was 10 cm, and the more clearly the leaf pattern of the crop was visually confirmed. The frequency difference was decreased, as the number of overlapped pixels was increased. Also the wave pattern of points where the crops were recognized were reduced.