• Title/Summary/Keyword: Non-labeling

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Rapid and exact molecular identification of the PSP (paralytic shellfish poisoning) producing dinoflagellate genus Alexandrium

  • Kim, Choong-jae;Kim, Sook-Yang;Kim, Kui-Young;Kang, Young-Sil;Kim, Hak-Gyoon;Kim, Chang-Hoon
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.132-133
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    • 2003
  • The marine dinoflagellate genus Alexandrium comprise PSP producing A. acatenella, A. angustitabuzatum, A. catenella, A. fundyense, A. minutum, A. ostenfezdii, A. tamiyavanichii and A. tamarense. In monitoring toxic Alexandrium, rapid and exact species identification is one of the significant prerequisite work, however we have suffered confusion of species definition in Alexandrium. To surmount this problem, we chose DNA probing, which has long been used as an alternative for conventional identification methods, primarily relying on morphological approaches using microscope in microbial field. Oligonucleotide DNA probes targeting rRNA or rDNA have been commonly used in diverse studies to detect and enumerate cells concerned as a culture-indetendent powerful tool. Despite of the massive literature on the HAB species containing Alexandrium, application of DNA probing for species identification and detection has been limited to a few documents. DNA probes of toxic A. tamarense, A. catenella and A. tamiyavanichii, and non-toxic A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax were designed from LSU rDNA D1-D2, and applied to whole cell-FISH. Each DNA probes reacted only the targeted Alexandrium cells with very high species-specificity within Alexandrium. The probes could detect each targeted cells obtained from the natural sea water samples without cross-reactivity. Labeling intensity varied in the growth stage, this showed that the contents of probe-targeted cellular rRNA decreased with reduced growth rate. Double probe TAMID2S1 achieved approximately two times higher fluorescent intensity than that with single probe TAMID2. This double probe did not cross-react with any kinds of microorganisms in the natural sea waters. Therefore we can say that in whole-cell FISH procedure this double DNA probe successfully labeled targeted A. tamiyavanichii without cross-reaction with congeners and diverse natural bio-communities.

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Binarization of number plate Image with a shadow (그림자가 있는 차량 번호판의 이진화)

  • Seo, Byung-Hoon;Kim, Byeong-Man;Moon, Chang-Bae;Shin, Yoon-Sik
    • Journal of Korea Society of Industrial Information Systems
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    • v.13 no.4
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    • pp.1-13
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    • 2008
  • In this paper, we propose a method to solve a problem in binarizing the rear number plate image captured by a camera on a moving vehicle. An image may be shadowed by the cavernous structure of the rear side of a moving vehicle and it makes us hard to get a high quality of binary image. Therefore, we first detect a shadow edge and then divide an image into the shadow part and non-shadow part by the edge. Finally, the binary image is obtained by binarizing each part and merging them In this paper, we do comparative work on a group of binarization methods including our method, the method suggested by Zheng, the method using block binarization, and the method using labeling. The result shows that our method achieves better performance than others in most cases.

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New screening method for anti-inflammatory agent (Cyclooxygenase 억제제 검색을 통한 항염증제 개발 연구)

  • Lee, Su-Hwan;Jeong, Seong-Won;Lee, U-Yeong
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.20 no.1
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    • pp.25-36
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    • 1994
  • It is weal known that bacterial lipopolysaccharide (LPS) stimulates prostaglandin synthesis in various experimental system via enhancing the expression of cylooxygenase-2 (COX-2). This study was designed to characterize U)5-induced prostaglandin synthesis in mouse peritoneal macrophages LPS-stimulated prostaglandin synthesis in macrophages with short term exposure was not so much prominent, but there was a burst in prostaglandin synthesis 8 hours after the LPS treatment and this u·as accompanied with the increase of cyclooxygenase activity, Dexamethasone markedly inhibited prostaglandin synthesis in this system. Metabolic label ins data supported above observations and thus, it could be concluded that LPS induces the do novo synthesis of COX-2 by which it stimulates the prostaglandin synthesis in mouse peritoneal macrophages, These data suggested that this experimental model system could be used for the screening procedure of COX-2 selective inhibitors. Ketoprofen, a non steroidal anti inflammatory agent, appeared to inhibit COX-1 relatively more selectively than COX-2.

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Preoperative Chemotherapy-Induced Apoptosis in Osteosarcoma (골육종에서 수술 전 항암화학요법에 의한 고사)

  • Kim, Ki-Soo;Choi, Yong-Soo;Yeom, Kyung-Sung;Shon, Kyung-Sun;Lee, Jae-Hyuk
    • The Journal of the Korean bone and joint tumor society
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    • v.5 no.4
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    • pp.221-228
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    • 1999
  • The purpose of this study was to verify the importance of apoptosis in genesis of osteosarcoma and whether apoptosis may play an important role as a predictive factor for the response to chemotherapy. Of the patients who were diagnosed osteosarcoma between January 1995 and June 1999, ten patients were selected. All specimens were obtained before and after preoperative chemotherapy and examined for the occurrence of apoptosis. Apoptosis was investigated by in situ end-labeling technique on paraffin-embedded sections and apoptotic indices were calculated before and after chemotherapy. The ages of ten patients ranged from 15 to 59 with equal sex ratio. All patients completed the planned pre-operative chemotherapy. Apoptosis occurs in osteosarcoma and apoptotic indices are increased after chemotherapy. Mean apoptotic index (AI) before and after chemotherapy were 17.2 (range 6-28.9) and 26.3 (9.6-46.2), respectively. Apoptotic cells were usually present around the necrotic area. The AI was increased as the progression of stage and in responder group more than in non-responder. Apoptosis is induced by pre-operative chemotherapy and the response is variable. Changes in AI levels before and after chemotherapy may possibly predict an individual patient's overall response.

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Survey Analysis of Familiarity and Willingness of the Use of Auxiliary Label in Community Pharmacists (근린약국약사를 대상으로 실시한 보조라벨의 이해도 및 사용의지에 관한 조사)

  • Choi, Byung-Chul;Hong, Myung-Ja;Choi, Han-Gon;Yong, Chul-Soon;Rhee, Jong-Dal;Yoo, Bong-Kyu
    • Korean Journal of Clinical Pharmacy
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    • v.16 no.1
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    • pp.9-13
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    • 2006
  • Patient counseling is emerging as one of the most important roles of community pharmacists because the information on the standard labeling for the prescription drug is not sufficient to ensure the correct use of the drug. However, excessive workload of the community pharmacists in Korea discourages the provision of the effective patient counseling. The use of auxiliary label may be an efficient tool to help patients correctly use the prescription drug in this situation. As a preliminary study to encourage the use of auxiliary label, we have performed a survey analysis of familiarity and willingness of community pharmacists to use the auxiliary label. About three quarters of the participating community pharmacists have heard of the auxiliary label, however, there was not a single pharmacist who uses the label. Furthermore, only one fifth of the participating pharmacists were willing to use the label if they have to purchase. Therefore, it is recommended that governmental and non-profit organizations such as Korean Pharmaceutical Association educate community pharmacists regarding usefulness of the auxiliary label with focus on enhancing patient compliance and constrainment of healthcare expense.

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The Housewives' Purchase Behaviors on Environment-friendly Agricultural Products in Daejeon Area (대전지역 주부의 친환경농산물 구매행동)

  • Kim, In-Jung;Lee, Joon-Ho
    • Korean Journal of Community Nutrition
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    • v.16 no.3
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    • pp.386-397
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    • 2011
  • This study was to investigate the housewives' purchase behaviors on the environment-friendly agricultural products (EFAP) by survey in Daejeon area housewives. 390 questionnaires were used and analysed. Most of the subjects were female (92.6%) distributed evenly in their 40's (55.4%), and graduated from high school (43.6%) or college (36.4%). Subjects' occupation was most housewives (64.1%) and 77.4% of the subjects had monthly family income of 2 million won or more. 76.9% of the subjects had purchased EFAP already. The reason of purchasing EFAP was mainly "good for health" (80.3%), and reason for non-purchasing was "high prices" (28%) or "not so trustworthy" (25.6%). The most purchasing frequency was "once a week" (29%). 46.7% of the subjects spent 20% of their agricultural product cost for EFAP and 38.7% of them spent less than 30,000 won per month for EFAP. On checking of EFAP labeling, the housewives scored 3.59 for the validate date, 3.25 for the place of origin, 2.8 for the quality certification mark by 4-point Likert scale. 65.1% of the subject had intention to increase purchasing of EFAP in future. To promote the consumption of EFAP, the improvement factors were price-cutting (47.9%), trust on producers (18.2%) and quality betterment (17.7%). Accordingly, the consumers prefer EFAP for wellbeing health of families; however, they hesitate to buy due to their high price and the low reliability on producers of EFAP. Thus the producers and the related organization of EFAP should contrive proper countermeasures to increase consumer's satisfaction level on their credibility and price of EFAP.

The Experimental Study of Effects of Irradiation on Osseointegration (방사선조사가 임프란트의 골유착에 미치는 영향에 관한 실험적 연구)

  • Park Kwan-Soo;Lee Sang-Rae;Hwang Eui-Hwan
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.29 no.1
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    • pp.119-147
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    • 1999
  • The purpose of this study was to investigate the effects of the Co-60 γ irradiation on the osseointegration. 2.0 mm titanium alloy screw implants(Sankin Industry Co. Ltd., Japan) were placed in the tibial metaphysis of the rabbits, bilaterally. The mean length of the implants was 6.0 mm. The right tibia was irradiated with a single dose of 15Gy from 60Co teletherapic machine at 5th postoperative day. The experimental group was irradiated tibia. The control group was non­irradiated tibia. To observe the phase of bone formation, the bone labeling by intramuscular injection of 20mg/Kg of Tetracycline, Calcein, Alizarin red S, was performed. The rabbits were sacrificed on the 1st, 2nd, 4th, 6th, 8th week and the tibia including implants were taken, and then the specimens were examined by the microradiography, light microscopy, and fluorescent microscopy. The obtained results were as follows: 1. There were connective tissue between bone and titanium at 1st week, in both group. Especially, the many empty lacunae without nucleus and obscure cytoplasm in experimental group, were observed. 2. The osteons were observed at 4th week in control group, and at 6th week in experimental group. The bone formation in experimental group was retarded as compared to the control group. 3. In fluorescent microscopy, bone labelling band was observed as linear, arc or concentric shape. Occasionary interrupted labelling band was observed, which is demonstrated bone remodeling. 4. In microradiographic examination, the radiolucent image was found between bone and implant with widening of bone marrow spaces as compared to the control group.

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Road Extraction by the Orientation Perception of the Isolated Connected-Components (고립 연결-성분의 방향성 인지에 의한 도로 영역 추출)

  • Lee, Woo-Beom
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.1
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    • pp.75-81
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    • 2012
  • Road identification is the important task for extracting a road region from the high-resolution satellite images, when the road candidates is extracted by the pre-processing tasks using a binarization, noise removal, and color processing. Therefore, we propose a noble approach for identifying a road using the orientation-selective spatial filters, which is motivated by a computational model of neuron cells found in the primary visual cortex. In our approach, after the neuron cell typed spatial filters is applied to the isolated connected-labeling road candidate regions, proposed method identifies the region of perceiving the strong orientation feature with the real road region. To evaluate the effectiveness of the proposed method, the accuracy&error ratio in the confusion matrix was measured from road candidates including road and non-road class. As a result, the proposed method shows the more than 92% accuracy.

Super-Pixels Generation based on Fuzzy Similarity (퍼지 유사성 기반 슈퍼-픽셀 생성)

  • Kim, Yong-Gil;Moon, Kyung-Il
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.2
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    • pp.147-157
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    • 2017
  • In recent years, Super-pixels have become very popular for use in computer vision applications. Super-pixel algorithm transforms pixels into perceptually feasible regions to reduce stiff features of grid pixel. In particular, super-pixels are useful to depth estimation, skeleton works, body labeling, and feature localization, etc. But, it is not easy to generate a good super-pixel partition for doing these tasks. Especially, super-pixels do not satisfy more meaningful features in view of the gestalt aspects such as non-sum, continuation, closure, perceptual constancy. In this paper, we suggest an advanced algorithm which combines simple linear iterative clustering with fuzzy clustering concepts. Simple linear iterative clustering technique has high adherence to image boundaries, speed, memory efficient than conventional methods. But, it does not suggest good compact and regular property to the super-pixel shapes in context of gestalt aspects. Fuzzy similarity measures provide a reasonable graph in view of bounded size and few neighbors. Thus, more compact and regular pixels are obtained, and can extract locally relevant features. Simulation shows that fuzzy similarity based super-pixel building represents natural features as the manner in which humans decompose images.

A Study of Core-Stateless Mechanism for Fair Bandwidth Allocation (대역 공평성 보장을 위한 Core-Stateless 기법 연구)

  • Kim, Hwa-Suk;Kim, Sang-Ha;Kim, Young-Bu
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.4C
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    • pp.343-355
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    • 2003
  • Fair bandwidth allocations at routers protect adaptive flows from non-adaptive ones and may simplify end-to end congestion control. However, traditional fair bandwidth allocation mechanisms, like Weighted Fair Queueing and Flow Random Early Drop, maintain state, manage buffera and perform packet scheduling on a per-flow basis. These mechanisms are more complex and less scalable than simple FIFO queueing when they are used in the interi or of a high-speed network. Recently, to overcome the implementation complexity problem and address the scalability and robustness, several fair bandwidth allocation mechanisms without per-flow state in the interior routers are proposed. Core-Stateless Fair Queueing and Rainbow Fair Queuing are approximates fair queueing in the core-stateless networks. In this paper, we proposed simple Layered Fair Queueing (SLFQ), another core-stateless mechanism to approximate fair bandwidth allocation without per-flow state. SLFQ use simple layered scheme for packet labeling and has simpler packet dropping algorithm than other core-stateless fair bandwidth allocation mechanisms. We presente simulations and evaluated the performance of SLFQ in comparison to other schemes. We also discussed other are as to which SLFQ is applicable.