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Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp. KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea

  • Kim, Ji Hyung;Affan, Abu;Jang, Jiyi;Kang, Mee-Hye;Ko, Ah-Ra;Jeon, Seon-Mi;Oh, Chulhong;Heo, Soo-Jin;Lee, Youn-Ho;Ju, Se-Jong;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.238-246
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    • 2015
  • A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 ± 0.2%, 48.8 ± 1.8%, and 22.2 ± 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.

Analysis of Occupational Disease Caused by Oral Health Behavior of Some Dental Hygiene Students (일부 치위생과 학생의 구강건강행위와 구강병 발생요인 간의 관계)

  • Lim, Soon-Hwan;Hwang, Ji-Min
    • The Journal of the Korea Contents Association
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    • v.18 no.3
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    • pp.256-264
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    • 2018
  • The purpose of this study was to investigate the relationship between oral health behaviors and the results of the oral dysentery test for dental hygienists and students at Gyeonggi - do and Chungcheongnam - do. Self - filling questionnaires and oral diseases. The following conclusions were obtained. The most common toothbrushing frequency was three times a day (60.2%), and the most dominant frequency of eating between meals was once or less(49.7%). The most common snack that they had was stickiness-free sweetened food(66.5%), and the type of beverage that they had the most was sweetened beverages(49.7%). The average stimulated saliva flow rate was 9.41ml, and they got a mean of 9.52 in the buffering capacity of saliva. The average glucose clearance time was a mean of 12.02. When they took a streptococcus mutans colony count test, 80.1 percent belonged to the low-risk group(<$10^5$), and 82.6 percent belonged to the low-risk group(<$10^5$) when a lactobacillus test was conducted. There was a positive correlation between the irritant saliva fraction and the non-irritating saliva and saliva buffering ability. In the fluoride application experience, the glucose retention time was 10.66 minutes and the fluoride application experience was 13.33 minutes. (P = .008). The importance of oral health, which is directly linked to general health, should be emphasized in order to improve the quality of life as well as the life expectancy. For oral health, which is directly linked to systemic health, it is necessary to provide opportunities for oral health education that can be easily accessed by the public, and to continuously develop and provide oral health care programs for a lifetime.

Characteristics of Volatile Fatty Acids Release During the Hydrolysis of Rice Straw (볏짚의 가수분해과정중 유기산 생성 특성)

  • Hong, Seung-Gil;Shin, JoungDu;Heo, Jeong-Wook;Park, Woo-Kyun;Shin, Hyun-Seon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.2
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    • pp.36-43
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    • 2012
  • Objective of this study was to investigate the effects of pre-treatment of rice straw by sizes(3cm, milled), temperatures($35^{\circ}C\;and\;55^{\circ}C$), with/without NaOH treatment, and RPM on the characteristics of volatile fatty acids production. The concentration of total volatile fatty acids (TVFAs) was increased with the hydrolyzed time. Concentration of VFAs in milled rice straw was higher than that in 3 cm cut. With the alkali treatment, the concentration of TVFAs were sharply increased, which showed 3 times higher than non-treatment. Concentration of VFAs was high at 150 rpm at $35^{\circ}C$, and at 80rpm and 200 rpm at $55^{\circ}C$. Among them acetic acid was dominant, which showed the similar increase with TVFAs. It was also observed that in the case of fibrous material, the contents of cellulose and hemi-cellulose were reduced a little, but no change in lignin.

Mineralogical Properties of Asian Dust Sampled at Deokjeok Island, Incheon, Korea in February 22, 2015 (2015년 2월 22일 인천광역시 덕적도에서 포집된 황사의 광물학적 특성)

  • Park, Mi Yeon;Jeong, Gi Young
    • Journal of the Mineralogical Society of Korea
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    • v.29 no.2
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    • pp.79-87
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    • 2016
  • Asian dust (Hwangsa) interacts with light, atmospheric gas, aerosol, and marine ecosystem, affecting Earth climate. Mineralogical properties are essential to understand the interaction between the dust and environments. In this study, we examined the mineralogical properties of Asian dust collected at Deokjeok Island, Incheon, Korea in February 22, 2015. X-ray diffraction (XRD) analyses showed that phyllosilicate minerals (62 wt%) dominate the Asian dust. Illite-smectite series clay minerals (55%) were common with minor chlorite (5%) and kaolinite (2%). Non-phyllosilicate minerals were quartz (18%), plagioclase (10%), K-feldspar (4%), calcite (4%), and gypsum (1%). Similar results were obtained by mineral quantification using scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS). Transmission electron microscopy combined with EDS confirmed illite-smectite series clay minerals as the dominant phyllosilicate type. Morphological analyses using SEM showed clay agglomerates, clay-coated quartz, feldspars, and micas. Gypsum grains were common on the particle surface, while calcite nanofibers, previously reported as common on the surface, were rare, indicating the reaction of calcite and acidic atmospheric pollutants to form gypsum. The analytical result of 2015 Asian dust would contribute to the establishment of mineralogical base for the modeling of the interaction between Asian dust and environments.

A Content Analysis of the test of the National Examination for Registration Nurses in Korea over 3 years (간호사 국가고시문제의 내용분석)

  • 서문자;윤순녕;유지수;송지호;최경숙
    • Journal of Korean Academy of Nursing
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    • v.26 no.1
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    • pp.73-93
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    • 1996
  • This study aimed to analyse the test contents of the national examination for the registered nurses (NERN) over 3 years from 1991 to 1993 in Korea. In recent years in Korea, the MCQ(multiple choice question) has been showing to be a highly recognized method for assessing the qualification of registered nurses. Unfortunately, nursing faculties have found NERN had some bad MCQs through having evaluation workshop for Some MCQs often provide so many unwriting clues which become a bias of the results, and some items fell into the category of the lower level of educational taxonomy such as isolated recall a fact or data. Frequently the stems of the questions are ambigous, unclear, disputable, esoterical or trivial. Considering those fallacies of the national examination, it is very critical to review the test items to see whether it is of high quality, is more fair, reliable and objective in depth. Therefore, this study was done to provide data for the improvement of the test contents as well as the teachers's assessment skill. For this study, the ad hoc committee was composed of 16 members, including 5 education board members of Korean Academic Nurses Association and 11 nursing faculty members. This committee had one day panel discussion and filled the checklist for this study. The process of analysing data was held over 10 times during 1992-1994. The analysis focussed on educational taxonomy such as cognitive domain(knowledge), psychmotor domain (skill), affective domain(attitude) and the level of learning such as recall, understanding, problems solving, and learning area of theory and practice, and the learning content categorised by nursing process and disease process. The test analysed using difficulty index and the structure of the test items was analysed. The conclusions and suggestion as follows : 1. In learning area, the average ratio of the theory and practice was 1 : 1.1 which was less than 1 : 2 suggested by Korean National Health Institute, and the ratio was different by the 8 leaning subjects of nursing. 2. In category of the educational taxonomy, the knowledge domain was emphasized mostly(79. 7%), the skill domain was 14.9%, and the attitude domain was 5.4% only. 3. In the level of learning, generally, the test items of the level of recall(45.5%) and the understanding(46.3%) were covered almost and the problem solving was 8.1%. 4. In the learning contents, generally, the test items related to nursing process was 67.2% and that of disease process was 32.8%. However, this proportion was different by the 8 leaning subjects. Even though the nursing diagnosis has been emphasized in nursing curricula recently, the test items of this was identified very few. 5. In the structure of the test item, some were not clear, incorrect grammar, unclear description and some have clues to answer. 6. In the item analysis, the non-acceptable level of the difficulty index (means too easy) was 65.7%, and the acceptable level was 33.9%. Considering the results we would like to suggest the followings, 1. Since the test items of knowledge domain was dominant, the test items of the practice domain and attitude domain should be emphasized more. 2. The regular review and analysis of NERN should be arranged in order to improve the quality of the test items which will give influence to the nursing education positively.

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Gene Polymorphism of XRCC1 Arg399Gln and Cervical Carcinoma Susceptibility in Asians: A Meta-analysis Based on 1,759 Cases and 2,497 Controls

  • Liu, Yi-Ting;Shi, Jing-Pu;Fu, Ling-Yu;Zhou, Bo;Wang, Hai-Long;Wu, Xiao-Mei
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.1
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    • pp.189-193
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    • 2013
  • Many epidemiological studies in Asian populations have investigated associations between the Arg399Gln gene polymorphism of X-ray repair cross complementing gene 1 (XRCC1) and risk of cervical carcinoma, but no conclusions have been available because of controversial results. Therefore a meta-analysis was conducted for clarification. Relevant studies were identified by searching the Pubmed, Embase, the Web of Science, Cochrane Collaboration's database, Chinese National Knowledge Infrastructure (CNKI), Wanfang database and China Biological Medicinse (CBM) until September, 2012. A total of eight studies were included in the present meta-analysis, which described 1,759 cervical carcinoma cases and 2,497 controls. Odds ratios (ORs) and corresponding 95% confidence intervals (95%CIs) as effect size were calculated by fixed-effect or random-effect models. The overall results indicated that the XRCC1-399G/A polymorphism was marginally associated with cervical carcinoma in Asians: OR (95%CI): 1.16 (1.07, 1.26) in the G/A vs G/G inheritance model, 1.24 (0.87, 1.76)in A/A vs G/G inheritance model, 1.13 (1.01, 1.27) in the dominant inheritance model and 1.18 (0.94, 1.47) in the recessive inheritance model. Subgroup analyses on sample size showed no significant correlation in the small-sample size group but the large-sample size group was consistent with the outcomes of overall meta-analysis. In the subgroup analysis by regions, we only found significant association under the G/A vs G/G inheritance model in the Chinese population. For the non-Chinese populations, no correlation was detected in any genetic inheritance model. In the Asian populations, XRCC1-399G/A gene polymorphism was implied to be associated with cervical carcinoma.

NEVOID BASAL CELL CARCINOMA SYNDROME : A REPORT OF CASE (기저세포모반 증후군 환아의 증례보고)

  • Heo, Su-Kyung;Choi, Nam-Ki;Kim, Seon-Mi;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.4
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    • pp.725-730
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    • 2008
  • Nevoid basal cell carcinoma syndrome is an ecto-mesodermal polydysplasia with numerous manifestations that affect multiple organs. The syndrome is an autosomal dominant inherited, with a high penetration and visible expression. The syndrome is characterized by a series of associated anomalies such as cutaneous, dentofacial, skeletal, ophthalmologic, neurological, and genital anomalies. Generally, the jaw cysts are multiple odontogenic keratocysts, affecting any area of maxilla and mandible. Multiple odontogenic keratocysts of this syndrome are more recurrent than the keratocysts of non-syndrome, thus they are treated aggressively for complete removal. We report a case of multiple jaw cysts associated with nevoid basal cell carcinoma syndrome. In clinical and radiological examinations, frontal bossing, hypertelorism, mild mental retardation and two odontogenic keratocysts in both the maxilla and mandible were observed. Two cysts were treated by marsupialization. For the management of eruption of unerupted teeth, periodic recall check and orthodontic treatment are required.

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From 'Medicalization' to 'Biomedicalization': the Case of Mental Disorder ('의료화'에서 '생의료화'로: 정신장애의 사례)

  • Kim, Hwan-Suk
    • Journal of Science and Technology Studies
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    • v.14 no.1
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    • pp.3-33
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    • 2014
  • Over the last forty years, the dominant perspective of social science on medicine has been the medicalization theory. It indicates the social process of expanding power of medical professionals by (re)defining the problems which were treated as non-medical phenomena(e.g. homosexuality, alcoholism, obesity, etc.) into "diseases" and thus the spheres of medical intervention. Meanwhile, rapid technoscientific changes in the medical field owing to the diffusion of biological sciences and information technologies since the mid-1980s and the accompanying emergence of new social arrangements such as bioeconomy and biological citizenship have led to the rise of a new social scientific perspective called the biomedicalization theory. This paper attempts to compare the two theories and assess their merits and demerits as a basic work to deepen the understandings of sociology and STS on contemporary medicine. And it also attempts to analyze their relative relevance through the case of mental disorder. The analysis on the case of mental disorder clearly shows that the medicalization in that area seems to have continuously proceeded since the early 19th centiry to the present. Furthermore, it also seems true that the five central processes of biomedicalization(except for risk surveillance technologies of mental disorder) have been observed and realized since the late 20th century. These results indicate that although medicalization has consistently proceeded, it has not been limited to the quantitative expansion of the medical field but been extended to the qualitative transformation asserted by the biomedicalization theory. Therefore, while the concept of medicalization is valid and significant even today, we can recognize that the concept of biomedicalization allow us to capture the new phenomena which cannot be properly and sufficiently captured by that of medicalization.

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Noise Analysis and Measurement for a CW Bio-Radar System for Non-Contact Measurement of Heart and Respiration Rate (호흡 및 심박수 측정을 위한 비접촉 방식의 CW 바이오 레이더 시스템의 잡음 분석 및 측정)

  • Jang, Byung-Jun;Yook, Jong-Gwan;Na, Won;Lee, Moon-Que
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.9
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    • pp.1010-1019
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    • 2008
  • In this paper, we present a noise analysis and measurement results of a bio-radar system that can detect human heartbeat and respiration signals. The noise analysis including various phase noise effects is very important in designing the bio-radar system, since the frequency difference between the received signal and local oscillator is very small and the received power is very low. All of the noise components in a bio-radar system are considered from the point of view of SNR. From this analysis, it can be concluded that the phase noise due to antenna leakage is a dominant factor and is a function of range correlation. Therefore, the phase noise component with range correlation effect, which is the most important noise contribution, is measured using the measurement setup and compared with the calculated results. From the measurement results, our measurement setup can measure a closed-in phase noise of a free-running oscillator. Based on these results, it is possible to design a 2.4 GHz bio-radar system quantitatively which has a detection range of 50 cm and low power of 1 mW without additional PLL circuits.

The Effects of the Biodiversity Increase after Creation of the Artificial Wetland -The Case of Ecological Pond at Seoul Technical High School- (인공습지 조성후 생물다양성 증진 효과에 관한 연구 -서울공고 생태연못을 중심으로-)

  • 김귀곤;조동길
    • Journal of the Korean Institute of Landscape Architecture
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    • v.27 no.3
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    • pp.1-17
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    • 1999
  • The purpose of this study is to evaluate the creation techniques of artificial wetland, one of biotopes developed to promote biodiversity in urban areas, and to look for improvement steps. Specifically, artificial wetland creation techniques were categorized into living environment and living creature classification. Being living conditions for creations, habitat environment was reviewed with a focus on water and soil environments. Living creatures were classified into plants, insects, fish, and birds. The evaluation of creation techniques was done in post-construction evaluation while considering the creation of habitats for living creatures. Intervention by users, changes in living environment and living species, and relevance of creation techniques were reviewed. Key results of this study are as follows. (1) Water environment for the living environment of creatures provides a suitable environment conditions for the living of creatures through a process easing the use of piped water. Various water depths and embankment appear to have a positive impact on the living of aquatic life. In particular, embankment covered in soil naturally played an important role as a place for the activities of aquatic insects and young fish as well as the growth of aquatic plants. (2) Various aquatic and ground plants to promote insect-diversity, shallow water, and old-tree logs had contributed greatly in increasing the types and number of insects. Aquatic insects. Aquatic insects were seen much particularly in areas where aquatic plants are rich but water is shallow than any other areas. (3) A space piled with stone to provide habitats for fish was not much used. However, it was observed that fish used embankment built with natural stones and embankment using logs in areas where water is deep. In addition, it was confirmed that 1,500 fish that had been released propagated using various depths and places for birth. (4) It was analyzed that techniques (creation of island, log setting, and creation of man-made bird nests) to provide habitats and to attract birds are not serving their roles. In such a case, it is believed that species had not increased due to the smallness as well as isolated features of the area. Based on theoretical review, they are judged to be areas that are likely to be used when a greater variety of birds is introduced. It is judged that attracting and keeping more birds at the site, such spaces need to be linked systematically in the future in terms of building eco-network while ensuring an adequate living areas. (5) In the study areas, users intervened greatly. As a result, a blockage was created preventing the normal growth of plants and non-indigenous plants were introduced. In order to limit the intervention by users, setting enough buffer zones, and environment education programs were urgently required. D/H=1>Hyangkyo> houses on the river>temples>lecture halls. D/H ratio of the backside areas is as follows. D/H=1>Hyangkyo>houses on the river>lecture halls. 4. Inner garden were planted deciduous than evergreen trees with Lagerstroemia indica. Enclosed dominant trees were planted by Pinus densiflora, Querces seuata. construct GEM strain, and examined for the expression and functional stability in microcosms.

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