• Mycobiology
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• 제46권4호
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• pp.407-415
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• 2018

Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types ($A5B4{\times}A1B4$). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated.

• Journal of information and communication convergence engineering
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• 제7권3호
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• pp.253-257
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• 2009

As the value of B2B integration gets more importance and the business process becomes more complex, BPEL4WS standard has been focused for the solution of the problems which is based on web service technology. Many vendors implement the standard in their systems to integrate B2B and web service based inner-Enterprise Application(EA)s. But there is no way to use the standard to integrate non-web service based EAs, because the standard is designed only for Service Oriented Architecture (SOA) over web services. This paper proposes Hybrid Service Orchestration Framework to participate web services and also non-web service based EAs in the business process as a partner using BPEL4WS standard. The framework introduces Hybrid Service Architecture to represent non-web service based EAs as a service, so BPEL4WS could be used to integrate web services and non-web service based EAs. And the framework also introduces Hybrid Service Toolkit to support software level facilities. The paper also introduces Hybrid Orchestration System that is based on Hybrid Service Orchestration Framework.

• Nuclear Engineering and Technology
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• 제43권2호
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• pp.187-194
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• 2011

Korea Nuclear Fuel is developing the X-GEN fuel which shows high performance and robust reliability for the worldwide supply. However, the simplified code systems such as CESEC-III which were developed in 1970s are still used in the current Non-LOCA safety analysis of OPR1000 and APR1400 plants. Therefore, it is essential to secure an advanced safety analysis methodology to make the best use of the merits of X-GEN fuel. To accomplish this purpose, the $\b{i}$ntegrated $\b{s}$afety $\b{a}$nalysis $\b{m}$ethodology (iSAM), is developed by selecting the best-estimate thermal-hydraulic code RETRAN. iSAM possesses remarkable advantages, such as generality, integrity, and designer-friendly features. That is, iSAM can be applied to both OPR1000 and APR1400 plants and uses only one computer code, RETRAN, in the whole scope of the non-LOCA safety analyses. Also the iSAM adopts the unique and automatic initialization and run tool, $\b{a}$utomatic $\b{s}$teady-$\b{s}$tate $\b{i}$nitialization and $\b{s}$afety analysis too l (ASSIST), to enable unhandy designers to use the new design code RETRAN without difficulty. In this paper, a brief overview of the iSAM is given, and the results of applying the iSAM to typical non-LOCA transients being checked during the reload design are reported. The typical non-LOCA transients selected are the single control element assembly withdrawal (SCEAW) accident, the asymmetric steam generator transients (ASGT), the locked rotor (LR) accident, and bank CEA withdrawal (BCEAW) event. Comparison to current licensing results shows a close resemblance; thus, it reveals that the iSAM can be applied to the non-LOCA safety analysis of OPR1000 and APR1400 plants.

• 한국식품과학회지
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• 제52권6호
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• pp.678-684
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• 2020

The objective of this study was to identify the fermentation characteristics of Bacillus thuringiensis and emetic, non-emetic Bacillus cereus using analytical profile index (API) test. Ten strains of B. thuringiensis and 10 strains of B. cereus including 3 strains of emetic type were used at the same concentrations. The differences of fermentation characteristics between the B. thuringiensis and B. cereus was not obvious, but the differences between the non-emetic and emetic B. cereus were distinctive. Seven among 50 substrates were negative for all non-emetic B. cereus strains and positive for all emetic strains, and three substrates among additional 12 substrates had the same tendency. From these differences, 3 emetic B. cereus strains were not indicated as B. cereus by API test. These results indicate that API test is not a suitable method to identify some strains of emetic B. cereus, and the distinctive differences in substrate utilization can be used to improve selective media.

• Journal of Nutrition and Health
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• 제27권9호
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• pp.923-929
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• 1994

The effects of vitamin B6 deficiency on energy utilization during fasting or underfeeding were studied in rats. Fifteen rats were fed a vitamin B6 deficient(-B6) diet and another 15 rats wee fed a control (+B6) diet. These rats were fed for 5 weeks with respective diet, and then subdivided into 3 groups : non-fasted group, fasted group, underfed group. Rats of the fasted group were fasted for 3 days and those of underfed group for 6 days. At the respective time (non-fast, 3 day-fast, 6 day-underfeed at 5 weeks), animals were sacrificed. Feed efficiency ratio of - B6 rats was significantly lower than that of +B6 rats. In - B6 rats, the liver and kidney weights were significantly heavier than those of +B6 rats but spleen and heart weights were not. In non-fasted group, liver protein and triglyceride level of - B6 rats were significantly higher than that of +B6 rats. After - B6 rats were fasted for 3 days, plasma free fatty acid level was significantly lower but liver glycogen level was higher than that of +B6 rats and muscle protein level of +B6 was decreased while that of - B6 was not changed. Vitamin B6 deficiency had little effect on the energy utilization with 6 days underfeeding. These results suggest that vitamin B6 deficiency may impair the utilization of stored fuel during fasting.

• BMB Reports
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• 제30권4호
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• pp.269-273
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• 1997

Heptocvte-specific expression induced by Hepatitis B virus (HBV) enhancer 2-core gene promoter was examined in various hepatocyte and non-hepatocyte cell lines. using non-viral and retroviral vector systems in which chloramphenicol acetyltransferase (CAT) is used as a reporter. The non-viral plasmid containing the HBV enhancer 2-core promoter exhibited 22 and 66% of CAT activities in hepatoma cell lines. HepG2 and Hep3B, respectively when compared with CAT activity expressed by CMV promoter. The CAT activities, however. were found to be marginal in other tested hepatoma cell lines as well as mouse primary hepatocytes and non-hepatocytes. The HBV enhancer 2 located upstream the CMV promoter did not affect the CMV promoter activity nor provided hepatocyte-specific expression. Transfection of retroviral plasmid DNA containing the HBV enhancer 2-core promoter as an internal promoter exhibited high and specific CAT expression in HepG2 and Hep3B cell lines but the activity value was 5 to 10 fold lower than the non-viral plasmid with identical promoter. These results suggest that the usage of HBV enhancer 2-core promoter for liver specific expression is limited to certain vectors and hepatocyte cell lines.

• 한국식품저장유통학회지
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• 제31권2호
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• pp.235-244
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• 2024

A nonthermally sterilized raw apple vinegar was manufactured using an ultra-fine filtration process (0.2 ㎛ membrane filter) and its quality was comparable to commercially available vinegar products. First, using apple concentrate as a raw material, it was possible to produce non-thermal sterilized Using a two-stage fermentation process of alcohol and acetic acid fermentations, a non-thermally sterilized raw apple vinegar with pH 2.94 and an acidity of 6.20% was produced from an apple concentrate. The fermentation process increased the browning index significantly. However, the fundamental quality parameters of the non-thermal sterilized raw apple vinegar (A) with sterilized apple vinegar (B) did not differ significantly. The pH (2.92-2.95) of apple vinegar (A and B) was higher than that (pH 2.65-2.70) of commercial vinegar (C and D), and the total acidity, which is in the range of 6.20-6.21% and 6.53-6.90%, respectively, was higher in samples C and D than in samples A and B. However, four kinds of organic acids were detected in non-thermal sterilized raw apple vinegar (A), and its total organic acid content (6,245.00 mg%) was significantly higher than that of other samples (B, C, D) (p<0.05). In particular, malic acid content, as a main organic acid in apples, was very high in sample (A) (244.83 mg%) and sample (B) (210.21 mg%), compared to commercial products C (125.78 mg%) and D (86.90 mg%). The total polyphenol content and antioxidant activity of fermented apple vinegar (A, B) were more than twice as high as those of commercial products (C, D). Vinegar A had higher total polyphenol content than vinegar B. The above results suggest it is possible to manufacture and commercialize non-thermal sterilized raw apple vinegar with higher organic acid content and antioxidant properties using ultra-fine filtration.

• 대한치과교정학회지
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• 제17권2호
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• pp.289-297
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• 1987

For the comparisons of dental archs and crowdings between musical wind instrument players groups and non-wind instrument players groups, 64 male wind instrument players and 31 non-wind instrument players group were selected. The results of the measurements were as follow. 1 Upper intercanine widths, upper basal arch widths, lower basal arch widths were statistically significant between musical wind instrument players groups (Class A, Class B) and non-wind musical instrument players group. 2 The mean value of upper intercanine width m Class A ($373{\pm}2.3mm$) was greater than than in Class B ($362{\pm}17mm$) and non-wind musical instrument players group($360{\pm}16mm$) 3 The mean value of lower basal arch width m Class B ($422{\pm}25mm$) was greater than that in non wind instrument players group ($395{\pm}28mm$). 4 The differences in the mean values of crowdings between Class A and Class Band non-wind instrument players group were not statistically significant.

• 한국농공학회논문집
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• 제60권6호
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• pp.73-81
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• 2018

In this study, subsurface discharge performance has been studied through theoretical seepage analysis on four types of culverts that can be installed under the condition of non-excavation, such as (a)perforated pipe(${\Phi}50mm$), (b)perforated pipe+horizontal mat (B50cm) (c)perforated pipe+horizontal mat+vertical gravel(B<10cm), (d)perforated pipe+vertical gravel(B<10cm), and existing typical type (e)perforated pipe with gravel (B40, h=40cm) which can be installed by excavation. The analysis results were as follows. i) Subsurface discharge performance per unit (m) was (a)type 56%, (b) 91%, (c) 96%, (d) 76%, respectively, lower than the value of (e)culvert. ii) However, considering that non-excavation culvert can be installed at a spacing of 5m with the installation cost of the existing excavation culvert at the interval of 10m, it was analyzed that unit subsurface discharge(q) of (a)20.2mm/day(110%), (b)32.8(178%), (c)34.6(188%) (d)27.5(149%) in the four types of non-excavation culvert installed at intervals of 5m under the condition of $ k=10^{-4}cm/s$ was much larger than the amount of (e)type 18.5(100%), existing excavation culvert installed at 10m interval. iii) Through the test construction, peak subsurface drainage discharge($q_p$) was 38.4mm/day, which is larger than the value of design criteria and confirmed that it satisfies the analysis results as well. iv) In particular, it was evaluated that (b)perforated pipe+horizontal mat(B50cm) are low cost, high efficiency subsurface drainage culvert type with sufficient drainage performance(178%).

• 보건행정학회지
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• 제9권1호
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• pp.110-138
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• 1999

This study intended to understand the factors influencing the utilization of charged retirement home services for the elderly by stage of discision making. At the same time the purpose of the study is also to present the results of this study as data for the expectation of the demand. The subject of this study was non-utilizer (635 persons) and utilizer (62 persons) of charged retirement home services over the age of sixty. Non-utilizer was selected by random sampling in urban and rural area, and utilizer was investigated by census survey in the three charged retirement home. The research method was interview survey by questionaire. The questionaire was composed of 59 items, 25 variables, 5 components. The research model was to add Andersen's Prediction Model. The components were predisposing component, enabling component, need component, actualizing component, psychologic component. The results of this study were as follows. 1)In the stage of recognizing the residence problem, the recognizer were 76.7% (487 persons) of all the non-utilizer. The factors of influencing were property (B=-4.1E-05), solidarity with children (B=-.1070), house satisfaction (B=-.2517), need of charged retirement home (B=.2614). 2)In the stage of selecting an altenative as utilizing of charged retirement home services, the selecter were 41.1% (261 persons) of all the non-utilizer. The factors of influencing were perception of charged retirement home (B=.2790), need of charged retirement home (B=.2971). 3)In the stage of decion-making, the decider were 29.6% (188 persons) of all the non-utilizer. The factor of influencing was need of charged retirement home (B=.3570). 4)In the stage of the actualization, the factors of influencing were charge ability of utilization (B=.1025), significant others (B=.1868). The upper results were implemented by using the statistical methods of frequency, t-test, $$\chi$^{2}$-test, multiple logistic regression ( and P 〈 .05 ).