• Maxillofacial Plastic and Reconstructive Surgery
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• 제36권3호
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• pp.85-93
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• 2014

Purpose: Nodal metastasis is the main prognostic factor in the patients with oral squamous cell carcinoma (OSCC). We investigated the association between tumor-associated lymphatics and OSCC characteristics. Methods: Thirty-four specimens were used for the immunohistochemical staining with the antibody for vascular endothelial growth factor (VEGF)-C, VEGF-D, VEGF receptor (VEGFR)-3, phosphorylated VEGFR-3, D2-40, and matrix metallproteinases (MMPs). We observed the distribution of the lymphangiogenic factors and quantified the degree of expression. We determined lymphatic vessel density (LVD) and lymphatic vessel dilatation with D2-40 immunostaining. We assessed the association of LVD or lymphatic vessel dilatation with tumor progression or tumor differentiation. Results: OSCC cells expressed lymphangiogenic ligands. Lymphangiogenic receptor, VEGFR-3, was expressed and activated in some tumor cells as well as in tumor-associated endothelial cells. LVD was not associated with tumor size or nodal status, but lymphatic vessel dilatation was higher in tumors with nodal metastasis, and also higher in poorly differentiated tumors. In stromal area of OSCC, MMP-1 and MMP-10 were up-regulated and the basement membrane of tumor-associated endothelial cells was destroyed by these collagenases. Conclusion: In the primary tumors with nodal metastasis, especially in poorly differentiated OSCC, tumor cells invaded the dilated lymphatic vessels via ruptured sites. MMP-1 and MMP-10 are important in the lysis of the glycocalyx inside the tumor-associated lymphatic endothelial cells.

• Molecules and Cells
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• 제37권5호
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• pp.389-398
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• 2014

Siah acts as an E3 ubiquitin ligase that binds proteins destined for degradation. Extensive homology between siah and Drosophila Siah homologue (sina) suggests their important physiological roles during embryonic development. However, detailed functional studies of Siah in vertebrate development have not been carried out. Here we report that Siah2 specifically augments nodal related gene expression in marginal blastomeres at late blastula through early gastrula stages of zebrafish embryos. Siah2 dependent Nodal signaling augmentation is confirmed by cell-based reporter gene assays using 293T cells and 3TP-luciferase reporter plasmid. We also established a molecular hierarchy of Siah as a upstream regulator of FoxH1/Fast1 transcriptional factor in Nodal signaling. Elevated expression of nodal related genes by overexpression of Siah2 was enough to override the inhibitory effects of atv and lft2 on the Nodal signaling. In particular, E3 ubiquitin ligase activity of Siah2 is critical to limit the duration and/or magnitude of Nodal signaling. Additionally, since the embryos injected with Siah morpholinos mimicked the atv overexpression phenotype at least in part, our data support a model in which Siah is involved in mesendoderm patterning via modulating Nodal signaling.

• 대한지리학회지
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• 제48권4호
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• pp.545-556
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• 2013

본 연구에서는 서울시 424개 단위지구의 쇼핑 위락통행의 결절지역을 설정하고 상층으로 연계되는 지역체계 특성을 도출하였다. 이를 위해 결절지역의 수직구조를 나타내는 고층인자분석을 이용하여 쇼핑 위락 통행을 분석한 결과, 쇼핑통행보다는 위락통행이 더 큰 비중을 차지하며 1차 계층의 각 결절지역은 주요 발착지가 중앙에 위치하고 인접한 주변 단위지구에 발지 또는 착지가 연계되는 패턴을 보인다. 인자의 고유치가 클수록 공간적 범위가 넓게 나타나며 외곽에 위치한다. 고층으로 갈수록 결절지역을 형성하는 공간적 범위가 넓게 나타나며, 3차 계층 이상에서 양극인자에 따른 비지적 연계와 강남 강북 간 연계가 확연해진다. 또한 일반적인 쇼핑 위락 중심지로 여겨지는 종로 중구, 강남구, 영등포구를 중심으로 하는 결절지역의 연계가 뚜렷하게 나타나지 않는다. 특히 종로 금천 결절지역은 2차 계층 이후 타 결절지역과의 독립성을 유지하면서 상층으로 연계된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4211-4214
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• 2015

Background: Prognostic value of prophylactic level VII nodal dissection in papillary thyroid carcinoma has been highlighted. Materials and Methods: A total of 27 patients with papillary thyroid carcinoma with N0 neck underwent total thyroidectomy with level VI and VII nodal dissection through same collar neck incision. Multicentricity, bilaterality, extrathyroidal extension, level VI and VII lymph nodes were studied as separate and independent prognostic factors for DFS at 24 months. Results: 21 females and 6 males with a mean age of 34.6 years old, tumor size was 5-24 mm. (mean 12.4 mm.), multicentricity in 11 patients 2-4 foci (mean 2.7), bilaterality in 8 patients and extrathyroidal extension in 8 patients. Dissected level VI LNs 2-8 (mean 5 LNs) and level VII LNs 1-4 (mean 1.9). Metastatic level VI LNs 0-3 (mean 1) and level VII LNs 0-2 (mean 0.5). Follow-up from 6-51 months (mean 25.6) with 7 patients showed recurrence (3 local and 4 distant). Cumulative DFS at 24 months was 87.8% and was significantly affected in relation to bilaterality (p-value <0.001), extrathyroidal extension (p-value <0.001), level VI positive ((p-value <0.001) and level VII positive ((p-value <0.001) LNs. No recurrences were detected during the follow-up period in the absence of level VI and level VII nodal involvement. Conclusions: Level VII prophylactic nodal dissection is an important and integral prognostic factor in papillary thyroid carcinoma. A larger multicenter study is crucial to reach a satisfactory conclusion about the necessity and safety of this approach.

• Nuclear Engineering and Technology
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• 제16권4호
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• pp.202-216
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• 1984

Nodal method가 소격격자 해석방법의 하나로 정립됨으로써, 계산격자가 비교적 크더라도 각 격자의 평균출력분포를 정확히 계산할 수 있게 하는 균질화변수틀 찾는 방법이 중요하게 되었다. 본 연구에서는 simplified equivalence theory와 approximate node equivalence theory의 두가지 근사방법을 가압경수형 원자로 문제에 적응하여 시험하여 보았다. 균질화계산과 노심분석계산 방법으로서 analytic nodal method에 기초를 둔 ANM 코드를 개발하였다. 여러 균질화 방법외 정확성을 KTDD 코드에 의한 reference solution과 비교하여 본 결과, 균질화 계산은 핵연료영역에서는 영역별 핵연료집합체 계산으로, baffle과 reflector의 공존 격자영역은 이들을 포함하는 color set 계산으로 수행할 수 있음을 알았다. Approximate node equivalence theory에 입각해서 approximate homogenized cross-section들과 approximate discontinuity factor들의 균질화 변수를 사용하면 출력분포와 임계도가 각각 0.8%, 0,1% 오차 범위내에서 예측되었다.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.97-102
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• 2004

This study was carried out to establish The regeneration of healthy seedlings from the nodal segment culture of Chinese yam (Dioscorea opposita cv. Danma), cultivated in Korea. Different explants such as leaves, petioles, roots and nodal pieces, excised from the in vitro grown seedlings of Chinese yam, were cultured on MS medium supplemented with various combinations of growth regulators. All the growth regulators used induced plantlet regeneration from the nodal segments at a high frequency, while there was no induction of shoot or callus from leaf, petiole or root tissues. The medium supplemented with 0.01mg/L NAA, 0.5mg/L BA, 0.5-1.0mg/L kinetin and without plant growth regulator was effective for shoot development of buds from the nodal segment culture. The concentration of BA and NAA was an important factor in the bud induction of buds from the nodal segments of Chinese yam. Nodal segments cultured on the medium containing 1.0mg/L NAA and 0.5-1.0mg/L BA gave the best response to bud formation. The addition of GA$_3$ to the culture medium suppressed shoot induction and growth, while it increased microtuber formation. The shoot growth and microtuber formation were also affected by medium strength and solidity. The MS basal medium containing 1 g/L gelrite was suitable for microtuber formation from the nodal segment of Chinese yam.

• Nuclear Engineering and Technology
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• 제51권5호
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• pp.1195-1208
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• 2019

This paper introduces a new two-step procedure for PWR depletion analyses. This procedure adopts the albedo-corrected parameterized equivalence constants (APEC) method to correct the lattice-based raw cross sections (XSs) and discontinuity factors (DFs) by accounting for neutron leakage. The intrinsic limitations of the conventional two-step methods are discussed by analyzing a 2-dimensional SMR with the commercial DeCART2D/MASTER code system. For a full-scope development of the APEC correction, the MASTER nodal code was modified so that the group constants can be corrected in the middle of a microscopic core depletion. The basic APEC methodology is described and color-set problems are defined to determine the APEC functions for burnup-dependent XS and DF corrections. Then the new two-step method was applied to depletion analyses of the SMR without thermal feedback, and its validity was evaluated in terms of being able to predict accurately the reactor eigenvalue and nodal power profile. In addition, four variants of the original SMR core were also analyzed for a further evaluation of the APEC-assisted depletion. In this work, several combinations of the burnup-dependent and -independent XS and DF corrections were also considered. The results show that the APEC method could enhance the nodal equivalence significantly with inexpensive additional costs.

• Nuclear Engineering and Technology
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• 제54권8호
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• pp.3059-3072
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• 2022

A Jacobian-Free Newton Krylov Two-Nodal Coarse Mesh Finite Difference algorithm based on Nodal Expansion Method (NEM_TNCMFD_JFNK) is successfully developed and proposed to solve the three-dimensional (3D) and multi-group reactor physics models. In the NEM_TNCMFD_JFNK method, the efficient JFNK method with the Modified Incomplete LU (MILU) preconditioner is integrated and applied into the discrete systems of the NEM-based two-node CMFD method by constructing the residual functions of only the nodal average fluxes and the eigenvalue. All the nonlinear corrective nodal coupling coefficients are updated on the basis of two-nodal NEM formulation including the discontinuity factor in every few newton steps. All the expansion coefficients and interface currents of the two-node NEM need not be chosen as the solution variables to evaluate the residual functions of the NEM_TNCMFD_JFNK method, therefore, the NEM_TNCMFD_JFNK method can greatly reduce the number of solution variables and the computational cost compared with the JFNK based on the conventional NEM. Finally the NEM_TNCMFD_JFNK code is developed and then analyzed by simulating the representative PWR MOX/UO₂ core benchmark, the popular NEACRP 3D core benchmark and the complicated full-core pin-by-pin homogenous core model. Numerical solutions show that the proposed NEM_TNCMFD_JFNK method with the MILU preconditioner has the good numerical accuracy and can obtain higher computational efficiency than the NEM-based two-node CMFD algorithm with the power method in the outer iteration and the Krylov method using the MILU preconditioner in the inner iteration, which indicates the NEM_TNCMFD_JFNK method can serve as a potential and efficient numerical tool for reactor neutron diffusion analysis module in the JFNK-based multiphysics coupling application.

• 한국공간구조학회논문집
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• 제13권1호
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• pp.87-96
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• 2013

This study investigated characteristics of buckling load and effective buckling length by member rigidity of dome-typed space frame which was sensitive to initial conditions. A critical point and a buckling load were computed by analyzing the eigenvalues and determinants of the tangential stiffness matrix. The hexagonal pyramid model and star dome were selected for the case study in order to examine the nodal buckling and member buckling in accordance with member rigidity. From the numerical results, an effective buckling length factor of adopted models was bigger than that of Euler buckling for the case of fixed boundary. These numerical models indicated that the influence of nodal buckling was greater than that of member buckling as member rigidity was higher. Besides, there was a tendency that the bifurcation appeared on the equilibrium path before limit point in the member buckling model.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1996년도 춘계학술발표회논문집(1)
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• pp.131-136
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• 1996

An analytic nodal expansion method has been derived for the multigroup neutron diffusion equation in 2-D cylindrical(R-Z) coordinate. In this method we used the second order Legendre polynomials for source, and transverse leakage, and then the diffusion eqaution was solved analytically. This formalism has been applied to 2-D LWR model. $textsc{k}$$_{eff}$, power distribution, and computing time have been compared with those of ADEP code(finite difference method). The benchmark showed that the analytic nodal expansion method in R-Z coordinate has good accuracy and quite faster than the finite difference method. This is another merit of using R-Z coordinate in that the transverse integration over surfaces is better than the linear integration over length. This makes the discontinuity factor useless.s.