Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Factors Affecting the Production of In Vitro Plants from the Nodal Pieces of Chinese Yam (Dioscorea Opposita Thunb)

  • Shin, Jong-Hee (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Kim, Sang-Kuk (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Kwon, Jung-Bae (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Lee, Bong-Ho (Institute of Bioresources, Gyeongbuk Provincial Agricultural Technology Administration) ;
  • Sohn, Jae-Keun (Dept. of Agronomy, Division of Bioscience, Kyungpook National University)
  • Published : 2004.06.01
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Abstract

This study was carried out to establish The regeneration of healthy seedlings from the nodal segment culture of Chinese yam (Dioscorea opposita cv. Danma), cultivated in Korea. Different explants such as leaves, petioles, roots and nodal pieces, excised from the in vitro grown seedlings of Chinese yam, were cultured on MS medium supplemented with various combinations of growth regulators. All the growth regulators used induced plantlet regeneration from the nodal segments at a high frequency, while there was no induction of shoot or callus from leaf, petiole or root tissues. The medium supplemented with 0.01mg/L NAA, 0.5mg/L BA, 0.5-1.0mg/L kinetin and without plant growth regulator was effective for shoot development of buds from the nodal segment culture. The concentration of BA and NAA was an important factor in the bud induction of buds from the nodal segments of Chinese yam. Nodal segments cultured on the medium containing 1.0mg/L NAA and 0.5-1.0mg/L BA gave the best response to bud formation. The addition of GA$_3$ to the culture medium suppressed shoot induction and growth, while it increased microtuber formation. The shoot growth and microtuber formation were also affected by medium strength and solidity. The MS basal medium containing 1 g/L gelrite was suitable for microtuber formation from the nodal segment of Chinese yam.

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References

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