To compare response of feeding concentrate mixtere and wheat straw (Group I) with either urea molasses liquid diet (UMLD) as sole ration (group II) or UMLD (similar to protein equivalent of concentrate mixture) plus wheat straw (group III) on intake and utilisation of nutrients and overall performance, twelve crossbred adult male cattle (Holstein Friesian ${\times}$ Hariana) aged about 2.5 years and weighing 342 kg were randomly allotted into three equal groups following completely randomised design and fed respective diets for a period of 60 days. Thereafter, a metabolism trial of seven days duration was conducted to assess nutrient utilisation and nitrogen balance. Animals fed UMLD as sole ration consumed significantly (p < 0.01) less amount of most of the nutrients as compared to other two groups, except crude protein, intake of which was higher in this group but it was comparable between II and III and III and I. On the other hand, digestibilities of nutrients were higher (p < 0.01) in group II, though it was comparable between other two groups, except ADF, the digestibility of which was lower in group II. Inspite of positive nitrogen balance in all the three groups, being significantly (p < 0.01) higher in group I, animals of group II and III lost their body weight especially to the utmost extent in group II, although the amount of energy (TDN) intake were similar statistically. Results indicate that during a scarcity period and economic compulsions, feeding of UMLD can be practised to replace only concentrate mixture from the maintenance ration of adult crossbred cattle for shorter duration as roughage part seems to be essential for the normal functioning of the rumen microbes and overall performance of the animals.
Xia, Chuanqi;Rahman, Muhammad Aziz Ur;Yang, He;Shao, Taoqi;Qiu, Qinghua;Su, Huawei;Cao, Binghai
Asian-Australasian Journal of Animal Sciences
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제31권10호
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pp.1643-1653
/
2018
Objective: This study investigated the effect of dietary crude protein (CP) supplementation on nutrient intake, nitrogen (N) utilisation, blood metabolites, ruminal fermentation and growth performance of young Holstein bulls. Methods: Twenty-one young bulls weighing $277{\pm}11.2kg$ were equally divided into three groups and were offered diets formulated with low CP (LCP; 10.21% CP and 4.22% rumen degradable protein [RDP]), medium CP (MCP; 12.35% CP and 5.17% RDP) and high CP (HCP; 14.24% CP and 6.03% RDP). Yellow corn silage was used as a unique forage source and was mixed with concentrate. This mixed feed was given ad libitum to the young bulls included in the study. Results: Results showed that CP intake, blood urea nitrogen, N intake, total N excretion and N balance increased linearly with an increase in dietary CP level (p<0.05). However, no significant difference was observed in nutrient digestibility among the bulls receiving the different diets. Ruminal pH (p<0.05) and ammonia nitrogen ($NH_3-N$) concentration (p<0.01) were significantly higher in the bulls receiving the MCP and HCP diets than in those receiving the LCP diet. The bulls receiving the HCP diet showed significantly higher ruminal bacterial protein level, propionate, acetate and total volatile fatty acid (TVFA) concentrations than bulls receiving the LCP diet (p<0.05). Moreover, dietary CP level exerted a significant positive effect on the final body weight, average daily gain and gain-to-feed ratio of the bulls (p<0.05). Conclusion: High dietary CP level is optimal for achieving maximum growth and high profitability without exerting a negative effect on the physiology of growing Holstein bulls.
The effect of urea treatment on chemical composition of neem seed kernel cake (NSKC) was investigated by soaking the cake in 2.1% urea solution (1.2 l $kg^{-1}$ NSKC) for five days. The effect on utilisation of nutrients by replacing groundnut cake (GNC) (30%) with urea-treated neem seed kernel cake (UTNSKC) (33%) in a concentrate mixture fed to meet 70% of the protein requirements of lambs (8 males and 8 females), was monitored in a digestibility study. Following urea treatment of NSKC only 9.5% of urea was hydrolysed and the crude protein content of the cake was increased by 6.65%. The tannin content in depulped neem seeds was 37% catechin equivalent. Whereas feeding UTNSKC had no effect on intake of dry matter (72.5 vs 66.3 g/kg $BW^{0.75}day^{-1}$) and digestibility of crude fibre (41.3 vs 43.4%), the cake depressed (p<0.01) the percent digestibility of dry matter (63.7 vs 70.2), crude protein (63.2 vs 70.2), nitrogen free extract (73.8 vs 80.5) and gross energy (64.3 vs 69.1). Digestibility of ether extract (75.8 vs 70.9%) was higher (p<0.05) in animals offered UTNSKC. The nutritive value of the composite ration consumed by lambs offered UTNSKC was lower (p<0.01) in terms of total digestible nutrients (64.7 vs 70.2%) and digestible energy (2.8 vs 3.0 Kcal/g DM). Intake of digestible energy (199.8 vs 194.1 Kcal/kg $BW^{0.75}day^{-1}$) and retention of nitrogen (7.53 vs 8.23 g $day^{-1}$) and calcium (2.12 vs 1.84 g $day^{-1}$) were comparable on the 2 rations. Animals fed UTNSKC retained less (p<0.01) phosphorus (0.37 vs 1.05 g $day^{-1}$). The results indicate that urea treatment increased the protein level of NSKC whereas feeding the treated cake as a replacement of GNC, lowered the digestibility of nutrients and retention of phosphorus.
Three male Gayal (Bos frontalis) and three male Yunnan Yellow cattle (Bos taurus) were fed pelleted lucerne and measurements made of digestibility, nitrogen utilisation, rumen fermentation and microbial population and key plasma metabolites. Total actual dry matter intake was similar but when expressed in terms of live weight or metabolic live weight feed intakes were significantly higher (p<0.05) for Gayal than cattle. Apparent digestibilities of dry matter, organic matter, fibre and dietary nitrogen were similar for both Gayal and cattle. Rumen ammonia nitrogen and total volatile fatty acids were significantly higher (p<0.05) for Gayal than cattle and total numbers of viable rumen bacteria, cellulolytic and amylolytic bacteria, but not proteolytic bacteria nor protozoa, were significantly greater (p<0.05) for Gayal than cattle. Although Gayal have a different rumen ecology to cattle, similar digestive parameters were exhibited. Further research is required to establish relationship between rumen ecology and digestive parameters.
Twenty crossbred calves of $88{\pm}5.5kg$ initial live weight and 3-4 month of age were divided into two groups and fed wheat straw and concentrate to support a 500 g daily gain in body weight. Calves in the experimental group (YC) were given a daily dose of 10 ml yeast cell suspension (YC) containing live cells $(5{\times}10^9 cells/ml)$ of Saccharomyces cerevisiae ITCCF 2094. After a growth study of 122 days metabolism trials were conducted. The calves in the YC group recorded a daily weigt gain of $492{\pm}27.8g$ as compared to $476{\pm}20.1g$ in control group. There were no significant differences in feed intake, nutrient digestibility, feed/gain ratio and nitrogen retention between the YC supplemented and control groups.
Selle, P.H.;Ravindran, V.;Pittolo, P.H.;Bryden, W.L.
Asian-Australasian Journal of Animal Sciences
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제16권8호
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pp.1158-1164
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2003
In two feeding experiments male and mixed-sex broiler chicks were offered diets based on sorghum and a wheatsorghum blend with two tiers of nutrient specifications, without and with microbial phytase (600 and 800 FTU/kg), from 7-25 and 1-42 days post-hatch, respectively. The nutrient specifications for protein, amino acids, energy density and phosphorus (P) of standard diets were reduced to formulate the modified diets on a least-cost basis. Calculated differences in nutrient specifications between standard and modified diets ranged from 14.3 to 17.1 g/kg crude protein, 0.24 to 0.40 MJ/kg apparent metabolisable energy (AME) and 1.06 to 1.20 g/kg available P. In both experiments, reduced nutrient specifications had a negative impact on growth rates and feed efficiency and phytase supplementation had a positive influence on growth performance and protein efficiency ratios (PER). Phytase addition to the less expensive, modified diets either partially or entirely compensated for reduced growth performance and, consequently, feed costs per kg of live weight gain were reduced. In Experiment 1, phytase increased (p<0.001) nitrogen-corrected AME (AMEn) from 15.39 to 15.89 MJ/kg dry matter. For nitrogen (N) retention there was an interaction (p<0.05) between diet type and phytase as the effects of phytase on N retention were more pronounced in the modified diets, with an increase from 0.512 to 0.561. These results demonstrate the positive effects of phytase on protein and energy utilisation, in addition to its established liberation of phytate-bound P and illustrate the feasibility of assigning nutrient replacement values to the feed enzyme for consideration in least-cost ration formulations. Further work is, however, required to define the most appropriate reductions in nutrient specifications in association with phytase supplementation.
Objective: This experiment investigated the effect of isonitrogenous replacement of groundnut cake (GNC) by rice gluten meal (RGM) and maize gluten meal (MGM) at 75% level on nutrient intake, apparent digestibility, growth performance and related blood constituents in growing Sahiwal cattle. Methods: Eighteen Sahiwal calves were divided into three groups, based on average body weight (87.24 kg) and age (6 to 12 mo), and treatments were assigned to the different groups randomly. The first group (GP-I) was kept as control and received GNC-based concentrate mixture. In second (GP-II) and third (GP-III) groups, 750 g/kg nitrogen (N) of GNC was substituted by RGM and MGM respectively, with similar forage:concentrate ratio (56:44). Results: The 90 days of experimental feeding revealed that intake of dry matter, crude protein and digestibility coefficients for all nutrients did not differ among groups. Furthermore, although N balance was greater ($p{\leq}0.05$) for GP-III than GP-I and GP-II, average daily gain was similar between GP-I and GP-II but greater ($p{\leq}0.05$) for GP-III. In addition, feed efficiency and related haematological variables did not differ due to treatments. Conclusion: Nutritional worth of GNC and RGM was highly comparable in terms of intake, digestibility and growth in growing calves. However, MGM was found to be more efficacious in improving growth rate than RGM at 75% replacement level of GNC protein.
Anaerobic digestion(AD) has successfully been used for many applications that have conclusively demonstrated its ability to recycle biogenic wastes. AD has been successfully applied in industrial waste water treatment, stabilsation of sewage sludge, landfill management and recycling of biowaste and agricultural wastes as manure, energy crops. During AD, i.e. organic materials are decomposed by anaerobic forming bacteria and fina1ly converted to excellent fertilizer and biogas which is primarily composed of methane(CH4) and carbon dioxide(CO2) with smaller amounts of hydrogen sulfide(H2S) and ammonia(NH3), trace gases such as hydrogen(H2), nitrogen(N2), carbon monoxide(CO), oxygen(O2) and contain dust particles and siloxanes. The production and utilisation of biogas has several environmental advantages such as i)a renewable energy source, ii)reduction the release of methane to the atomsphere, iii)use as a substitute for fossil fuels. In utilisation of biogas, most of biogas produced from small scale plant e.g. farm-scale AD plant are used to provide as energy source for cooking and lighting, in most of the industrialised countries for energy recovery, environmental and safety reasons are used in combined heat and power(CHP) engines or as a supplement to natural. In particular, biogas to use as vehicle fuel or for grid injection there different biogas treatment steps are necessary, it is important to have a high energy content in biogas with biogas purification and upgrading. The energy content of biogas is in direct proportion to the methane content and by removing trace gases and carbon dioxide in the purification and upgrading process the energy content of biogas in increased. The process of purification and upgrading biogas generates new possibilities for its use since it can then replace natural gas, which is used extensively in many countries, However, those technologies add to the costs of biogas production. It is important to have an optimized purification and upgrading process in terms of low energy consumption and high efficiency giving high methane content in the upgraded gas. A number of technologies for purification and upgrading of biogas have been developed to use as a vehicle fuel or grid injection during the passed twenty years, and several technologies exist today and they are continually being improved. The biomethane which is produced from the purification and the upgrading process of biogas has gained increased attention due to rising oil and natural gas prices and increasing targets for renewable fuel quotes in many countries. New plants are continually being built and the number of biomethane plants was around 100 in 2009.
Selle, P.H.;Ravindran, V.;Ravindran, G.;Pittolo, P.H.;Bryden, W.L.
Asian-Australasian Journal of Animal Sciences
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제16권3호
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pp.394-402
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2003
Individual and combined supplementation of phosphorus-adequate, wheat-based broiler diets with exogenous phytase and xylanase was evaluated in three experiments. The effects of the enzyme combination in lysine-eficient diets containing wheat and sorghum were more pronounced than those of the individual feed enzymes. The inclusion of phytase plus xylanase improved (p<0.05) weight gains (7.3%) and feed efficiency (7.0%) of broilers (7-28 days post-hatch) and apparent metabolisable energy (AME) by 0.76 MJ/kg DM. Phytase plus xylanase increased (p<0.05) the overall, apparent ileal digestibility of amino acids by 4.5% (0.781 to 0.816); this was greater than the responses to either phytase (3.6%; 0.781 to 0.809) or xylanase (0.7%; 0.781 to 0.784). Absolute increases in amino acid digestibility with the combination exceeded the sum of the individual increases generated by phytase and xylanase for alanine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, phenylalanine, threonine, tyrosine and valine. These synergistic responses may have resulted from phytase and xylanase having complementary modes of action for enhancing amino acid digestibilities and/or facilitating substrate access. The two remaining experiments were almost identical except wheat used in Experiment 2 had a higher phytate concentration and a lower estimated AME content than wheat used in Experiment 3. Individually, phytase and xylanase were generally more effective in Experiment 2, which probably reflects the higher dietary substrate levels present. Phytase plus xylanase increased (p<0.05) gains (15.4%) and feed efficiency (7.0%) of broiler chicks from 4-24 days post-hatch in Experiment 2; whereas, in Experiment 3, the combination increased (p<0.05) growth to a lesser extent (5.6%) and had no effect on feed efficiency. This difference in performance responses appeared to be 'rotein driven'as the combination increased (p<0.05) nitrogen retention in Experiment 2 but not in Experiment 3; whereas phytase plus xylanase significantly increased AME in both experiments. In Experiments 2 and 3 the combined inclusion levels of phytase and xylanase were lower that the individual additions, which demonstrates the benefits of simultaneously including phytase and xylanase in wheat-based poultry diets.
Wheat straw was treated on laboratory scale with 4% urea at a moisture level of 50% along with different amount of HC1 to fix various levels of ammonia (30, 40, 50 and 60%) and stored for 4 weeks. Result, revealed a significant (p<0.01) increase in CP content of the samples where HC1 was added. The CP content of the straw was only 7.8%, which increased to 14.1, 16.0, 15.0 and 15.2% with the addition of acid. Similarly the concentrations of NDF, ADF and hemicellulose was significantly different due to HC1 addition. The level of HC1 recommended was to trap 30% ammonia as there was not significant difference in CP content of straw due to addition of 4 levels of acids. Results of in vivo experiment conducted on nine buffaloes divided randomly into three groups of three animals in each revealed no significant difference in the intake of DM, OM, NDF, ADF, cellulose and hemicellulose in group I (ammoniated straw), group II (HC1 treated ammoniated straw) and group III (HC1 treated ammoniated straw + 1 kg barley grain), but the intake of CP was significantly (p<0.01) more in group III as compared to other 2 groups. The digestibility of DM, OM and CP was significantly (p<0.01) more in groups where HCI treated straw was fed as compared to only ammoniated straw fed group, whereas there was no significant difference in the digestibility of NDF, ADF and cellulose in 3 groups. Intake was significantly higher of nitrogen (p<0.05), calcium (p<0.01) and phosphorus (p<0.01) in group III as compared to other two groups. Animals in all the 3 groups showed positive nitrogen, calcium and phosphorus balance, though the balances of all the 3 nutrients were significantly higher in group III as compared to other 2 groups. Rumen fermentation study conducted in 3 rumen fistulated buffaloes in $3{\times}3$ latin square design offering the same 3 diets as in group I to III revealed that rumen pH was alike statistically in 3 groups and at various time intervals. The mean ammonia-N concentration was significantly (p<0.01) more in group II and III as compared to group I. The mean TVFA concentration (mM/100 ml SRL) were 6.46, 7.84 and 8.47 in 3 groups respectively and different statistically (p<0.01). Results revealed no significant difference in the activities of carboxy methyl cellulase, urease or protease at both the time of sampling (0 h and 4 h) in all the 3 groups of animals.
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