• Animal Bioscience
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• 제35권10호
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• pp.1556-1565
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• 2022

Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters. Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism. Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a^* and b^* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency. Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs.

• Journal of Nutrition and Health
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• 제18권3호
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• pp.194-200
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• 1985

The purpose of this study was to investigate sodium intake and protein metabolism for pregnant women in Korea. The relationship between salt consumption and hypertension, electrolyte changes in pregnancy, and the tendency of weight gain of pregnant women were also examined. The dietary survey and twelve or twenty-four hour's urine collection was performed in this study. The subjects were pregnant women who came to gynecology clinic for prenatal care from July to August in 1984. The results were as follows: 1) Intakes of calorie and iron of Korean pregnant women were 2,036 Kcal and 16mg per day per person. The calorie composition consists of 65% carbohydrate, 16% protein, and 19% lipids. The average level of all nutrients was about 87% of Korean Recommended Dietary Allowances. 2) The sodium intakes in pregnant women estimated by dietary survey was 318.6 mEq Na (7,966 mg Na), which is equivalent to 20.2 g NaCl. 3) Daily sodium excretion was 112.5 mEq per liter, that is equal to 147.4 mEq. Potassium excretion was 24.95 mEq per liter and creatinine excretion was 594.0 mg per day. 4) There were statistically significant correlations between sodium intake and the excretions of sodium, potassium and creatinine. The blood pressure was positively correlated with sodium intakes. As sodium and potassium ratio was increased, systolic blood pressure and diastolic blood pressure were also significantly increased 5) Urinary nitrogen and creatinine ratio known to be reliable index for nutritional status of protein was 5.5 : 1. These values were comparable to the values in American pregnant women. 6) The weight gain during the pregnancy was examined. There was little weight gain during the first trimester, because of nausea, vomit and decrease of appetite. The weight gain during the second trimester was 4.9Kg, and 11.1Kg for third trimester.

• 한국식품영양과학회지
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• 제2권1호
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• pp.23-30
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• 1973

Wistar strain male rats were divided into four different diet groups and the control group was fed on 7-percent casein diet (C), the second with a mixture of 80% of rice and 20% of buckwheat (RW), the third with rice only (R), and the fourth with a mixture of rice and buckwheat of equal nitrogen level (RWS). Each diet group consisted of 6 rats was fed for three weeks by the ad libitum feeding method. The results of the experiment, i.e., the growth gain and change in the level of free amino acids in the plasma and liver determined by the micro-bioassay method were as follows. 1. The group C showed the largest growth gain and the rest did in the order of RW group, R group. and RWS group. 2. Rats fed on diet of high protein score showed high level of free amino acids tryptophan, lysine, and threonine in the plasma and liver except for the case of lysine in the liver. 3. Protein score would be estimated by the level of free amino acids in the liver and plasma.

• The Korean Journal of Ecology
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• 제27권3호
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• pp.155-159
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• 2004

In order to gain a better understanding of how forests participate in the cycling of carbon, effects of nitrogen addition on soil respiration were investigated on the oak forest in Kongju, Korea. Study site was divided into control, treatment f and treatment 2 plots, with 5 replication in each plot. In each replicate of treatment 1 and treatment 2 were fertilized with ammonium nitrate (NH$_4$NO$_3$), 30 g/$m^2$ and 60 g/$m^2$, respectively. Soil respiration, soil temperature, ammonium-N and nitrate-N were measured during the experimental period. Ammonium-N and nitrate-N in Ta were higher than those in control and T$_1$. Ammonium-N and nitrate-N in top-soil and sub-soil decreased sharply in August after bi9 rainfall in July in T$_1$ and T$_2$, however, those in control plot increased. Soil respiration in T$_2$ Plot showed consistently higher than those in control and T$_1$ until the end of July. However, soil respiration was similar among the control, T$_1$ and T$_2$ in mid-August and September The amount of Co$_2$ released from soil respiration in control, T$_1$ and T$_2$ in mid-August was 8.0$\pm$0.4, 9.3$\pm$0.6 and 10.2$\pm$0.5 $\mu$mol$^{-1}$ ㆍm$^{-2}$ ㆍs$^{-1}$ , respectively. However, those in control, T$_1$ and T$_2$in mid-August was 13.0$\pm$0.4, 13.5$\pm$0.5, 13.3$\pm$0.6 $\mu$mol$^{-1}$ ㆍm$^{-2}$ ㆍ$^{-1}$ , respectively. The results suggest that nitrogen addition in this oak forest has a positive effect on soil respiration.

• Journal of Nutrition and Health
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• 제2권1호
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• pp.35-46
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• 1969

임신 전(前) Restricted diet을 먹은 것과 Unrestricted diet을 먹은 group간에 2세의 체중증가에 미치는 영향에는 차이가 없었으며 임신 전(前) 체중변화에 있어서 Restricted diet에 숫컷이 더 예민하였다. 암컷에서는 Recovery diet을 주는 기간에는 Unrestricted group을 거의 쫓아 갔으나 숫컷에서는 그차이가 심하였다. 새끼들의 체중에 있어서 임신동안의 초기와 후기의 식이제한 group은 관계없이 임신전(全)기간 식이제한 group과 Unrestricted group간에 체중의 차이를 나타내었다. 어미의 Nitrogen 배설량에서 보면 섭취량에 대한 배설량의 백분율은 사료섭취량의 증감(增減)에 관계없이 일정하며 배설량은 사료섭취량이 많으면 많이 나가고 적으면 그것에 비례해서 적게 배설되었다. 새끼의 Nitrogen 배설량은 8 group사이에 큰 차이를 보여주지 않고 있다. Birth weight는 임신전(前) Restricted diet이나 Unrestricted diet 영향을 받지 않았으며 임신동안의 Unrestricted group의 새끼가 임신동안 전(全)기간 식이제한을 하였거나 부분적으로 식이제한을 한 group의 새끼보다 Birth weight가 컸다. litter size는 사료섭취량에 영향을 받지 않았다.

• 대한가정학회지
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• 제20권2호
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• pp.91-102
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• 1982

This study was designed to find out the nutritional defect of general Korean diet and the effective way of nutritional supplementation. Seventy weanling Sprague-Dawley male rats weighing 51.8$\pm$0.9g were blocked into ten groups and fed ten different diets ad libitum for eight weeks: Standard groups(st gp) was given 72% sugar-20% casein diet: Cereal-vegetable group(c-v gp) was fed cereal-vegetable diet(c-v diet) composed of rice, barley, soybean, spinach and cabbage: the other eight groups were fed c-v diets supplemented with casein, vitamin $B_{2}$, calcium, vitamin A, vitamin B2 and A, vitamin A and calcium, vitamin $B_{2}$ and calcium, vitamin A and $B_{2}$and calcium, respectively, on the basis of each nutrients content of standard diet. The results were as follows: 1. Food intakes and body weight gains in all the experimental groups were significantly lower than st gp. Among experimental groups, casein gp and vit B2 gp tended to gain more body weights than c-v gp. 2. Through all the experimental period, F.E.R., pp.E.R., and NDPcal% did nod show significant differences among all the experimental groups and st gp. 3. The weights of liver, kidney, and gastrocnemius were significantly lower in all the experimental groups as compared with st gp. But brain and sex organ weights did not show differences among all the groups. 4. Nitrogen contents of total carcass, liver, and gastrocnemius in all the experimental groups tended to be increased as compared with st gp, and among experimental groups, they tended to be increased by casein supplementation and decreased by ca supplementation. 5. Apparent nitrogen digestibility, urinary and fecal nitrogen excretion, the amount of nitrogen retained, and N.P.U. did not show any significant differences among all the groups. 6. Serum total protein concentrations did not show any significant differences among all the groups.

• Journal of Nutrition and Health
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• 제15권4호
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• pp.301-312
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• 1982

섭취한 식품이나 에너지를 체내에 보유하는데에 효율적인 것으로 알려진 meal feeding급식형태를 이용하여 단백질의 종류와 수준을 달리했을 때 쥐의 성장, 단백질 및 에너지 대사, 체조성에 미치는 영향을 파악코저 하였다. Casein과 ISP를 10, 20 및 30% 수준으로 달리하고 에너지수준을 3600 kcal ME/kg으로 동일하게구성된 실험사료를 82-100 g되는 숫쥐에게 ad libitum feeding및 1 일 2회 2시간씩 meal feeding으로 4주간의 성장시험과 대사시험을 실시하였던 바 결과는 다음과 같다. 1) Meal fed 시켰을때 사료섭취량은 ad libitum보다 $66{\sim}86%$로 저하되었으며, 증체량 역시 저하되었다. 그러나 casein 20%와 30% 수준에서는 ad libitum 사료섭취량의 85%에 불과했으나 동일한 증체량을 보였다. 2) 사료효율 및 에너지효율은 급식형태에 의한 통계적 유의차를 인정할 수 없었으며, 특히 casein 20%와 30% 수준에서는 사료및 에너지효율이 ad libitum 보다 me fed시켰을 때에 더 높았다. 3) 총에너지 섭취량, 가소화에너지 및 대사에너지는 ad libitum보다 meal fed시켰을 때에 약간 감소되는 경향이었고 에너지의 소화율이나 이용율은 동일했다. 4) 질소섭취량은 ad libitum보다 meal fed시켰을 때에 통계적으로 유의성 (P<0.05) 있게 적었음에도 불구하고, 질소균형과 질소보유율의 차이에는 유의성을 보이지 않았다. 질소균형 및 보유율은 단백질 10% 수준에서는 ad libitum에서 더 높은 경향이었으나 20%와 30% 수준에서는 오히려 meal fed 시켰을때에 더 높은 경향이었다. 5 ) 7주간의 시험완료 후 도체분석에 의한 체조성은 급식형태에 의한 유의차가 인정되지 않았다. 체지방량과 체수분량과는 음의 상관관계 (r = -0 .77)를 보였다. 이상의 결과에 의하면, casein의 경우, 20 % 와 30 % 수준에서 meal feeding에 의한 효과를 확인하였으며 이는 증체의 효과, 사료및 에너지의 이용을 증진의 효과, 질소보유율 증가의 효과, 체지방 축적의 경향을 보였다. 이 같은 경향은 casein군이 ISP군보다 더 효과적이었다.

• Journal of Animal Science and Technology
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• 제51권2호
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• pp.155-162
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• 2009

본 연구는 진도성견의 단백질 급여수준이 질소균형에 미치는 영향을 조사하여 단백질 급여 표준을 설정하기 위한 기초 자료를 얻기 위하여 실시하였다. 진도견 성견초기(생후 36~38주령)와 성견후기(생후 45~49주령)의 암컷 12두를 대상으로 단백질 수준을 17, 19 및 21%로 설정한 실험사료를 급여하여 체중, 사료섭취량 및 분뇨 배설량을 측정하였다. 성견초기의 일당 증체량은 조단백질 함량 17, 19 및 21%구에서 각각 -38.33, -16.25 및 -12.71g으로 나타났으며 17% 조단백질 사료급여구가 19 및 21% 급여구에 비하여 유의하게 낮았다(p<0.05). 축적된 질소량은 각각 -0.29, -0.04 및 -0.03g/kg$BW^{.75}$/d로 계산되었으며 조단백질 17% 급여구가 19 및 21% 급여구에 비하여 유의하게(p<0.05) 낮은 결과를 나타내었다. 질소섭취량(x)과 질소축적량(y)간에 y = 0.7484x-1.18 ($R^2$ = 0.9923)의 회귀식이 도출되었으며, 이 식으로부터 성견초기의 유지 조단백질 요구량은 9.85g/kg$BW^{.75}$/d로 추정되었다. 진도견 성견후기의 일당 증체량은 실험구 별로 각각 -34.05, -28.71 및 -28.28g으로 17% 단백질 사료급여구가 다른 구에 비하여 낮게 나타났으나 유의한 차이는 아니었다. 축적된 질소량은 각각 -0.33, -0.06 및 -0.09g/kg$BW^{.75}$/d로 계산되었으며 17% 조단백질 급여구가 19 및 21% 급여구에 비하여 유의하게(p<0.05) 낮은 결과를 나타내었다. 질소섭취량(x)과 질소축적량(y)간에 y = 0.8823x-1.0894 ($R^2$ = 0.9982)의 회귀식이 도출되었으며 이식을 이용하여 계산한 결과 성견후기의 유지 조단백질 요구량은 7.72g/kg$BW^{.75}$/d로 추정되었다. 본 연구는 우리나라에서 진도견을 대상으로 한 영양소 요구량을 설정하기 위한 최초의 연구로 생각되며 연구의 결과는 진도견의 단백질 요구량을 설정하는데 기초 자료가 될 것으로 생각된다. 그러나 진도견의 단백질 요구량을 최종적으로 설정하기 위해서는 전 생애에 걸쳐서 성별 및 사육환경에 따른 영양소 요구량에 대한 추가적인 연구가 필요하다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권4호
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• pp.554-557
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• 1999

Hatchery waste including infertile eggs, dead embryos in shell, dead or low grade chicks was cooked at $100^{\circ}C$ for 15 minutes and then oven dried and ground. Hatchery waste meal (HWM) thus prepared contained 44.25% protein, 4,573 kcal/kg gross energy, 3,600 kcal/kg metabolizable energy, 30% ether extract, 1.9% fibre, 14% ash, 9.8% nitrogen free extract, 7.26% Ca, 0.84%P, 1.86% lysine and 0.66% methionine with no Salmonella and E. coli. In biological evaluation trial, significantly higher weight gain was observed in ration containing 12% HWM compared with that containing similar amount of fish meal. Protein efficiency ratio on the two rations was 3.96 vs 2.85; protein digestibility, 86.02 vs 71.9; net protein utilization, 64.9 vs 42.37 and biological value, 75.37 vs 58.84, respectively, indicating better balance of amino acids in HWM compared with fish meal. Growth performance trial on broiler chicks also revealed better weight gain and feed efficiency on ration containing 12% HWM than that containing similar level of fish meal.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.946-951
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• 2011

The study was conducted to evaluate enzose (corn dextrose), a corn milling byproduct, as substitute for corn grain as energy in growing lambs. Five iso-caloric and iso-nitrogenous diets were formulated. The control diet (E0) had no enzose whereas enzose replaced 20, 40, 60 and 80% corn grain in E20, E40, E60 and E80 diets on the basis of energy supply, respectively. Fifty growing lambs were divided into 5 groups, 10 animals in each, in a randomized complete block design. Nutrients (dry matter, crude protein, neutral detergent fiber and acid detergent fibre) intake and digestibilities increased with gradual replacement of corn grain by enzose. Lambs fed E80 diet also retained higher nitrogen (N) than those fed E0 diet. Plasma glucose, $T_3$ and $T_4$ increased while urea N decreased in lambs receiving higher enzose content. Maximum weight gain was recorded in lambs fed diets containing maximum concentration of E as a replacement for corn grains. A better feed conversion ratio was recorded in lambs fed E80 compared with those fed E0 diet. The study suggests that enzose can be used as an economical feed ingredient to replace corn grain upto 80%, without any adverse effects on growth performance of growing lambs.