• 한방안이비인후피부과학회지
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• 제21권2호
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• pp.54-70
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• 2008

Background and Object : This study was carried out to investigate the effects of GCSBPT (Gagam-Cheongsang BangPungTang) on the in vitro and in vivo anti-inflammatory reactions. Methods : Vascular permeability and Cyclooxygenase inhibition assay are examined in vitro and nitric oxide inhibition assay, radical scavenging activity test, $TNF-{\alpha}$, COX-2 inhibition test are examined in vivo. Results : GCSBPT showed inhibitory effects on vascular permeability and leukocyte migration in animal test. In cyclooxygenase 2 inhibition assay, an ethanol extract of GCSBPT inhibited prostaglandin E2 generation at a concentration of $10{\mu}g/ml$. Among the herbal ingredients of GCSBPT, ethanol extracts of Nepetae Spica exhibited potent inhibitory activities. Ethanol extract of GCSBPT inhibited the release of nitric oxide and the gene expression of inducible nitric oxide synthase in RAW 246.7 cells stimulated by lipopolysaccharide. Ethanol extract of GCSBPT exhibited radical scavenging activity of 54% at $100{\mu}g/ml$. Among the herbal ingredients of GCSBPT. Conclusions : According to the above results, I expected that GCSBPT was a potent anti-inflammatory prescription.

• 동의생리병리학회지
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• 제20권2호
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• pp.414-419
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• 2006

Prostaglandins biosynthesis and nitric oxide production have been implicated in the process of inflammation and osteoarthritis. And nitric oxide (NO) activated the MMPs responsible for PG degradation in articular chondrocytes. Therefore, we have studied the effects on anti-inflammation and analgesic by ethyl acetate fraction from 70% ethanol extract of Perilla Frutescens (EPF). EPF inhibited LPS plus inflammation-cytokines-induced proteoglycan (PG) degradation, matrix-metalloproteinase (MMP-2,9) expression in rabbit articular chondrocytes. Also, EPF have inhibitory effects on LPS or LPS plus inflammation cytokines-induced nitric oxide (NO) and PGE2 production in mouse macrophage andrabbit articular chondrocytes. These results suggest that EPF decreases PGE2, iNOS, MMPs activity and PG degradation in mouse macrophage and/or rabbit articular chondrocytes. In vivo, EPF was shown to have inhibitory effects on acetic acid-induced pain. The herbal extract with this profile, may have utility in the treatment of osteoarthritis.

• 생약학회지
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• 제43권1호
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• pp.85-93
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• 2012

181 kinds of local native plants were selected by its anti-inflammatorial folk medicinal uses and evaluated it antioxidative and inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced 264.7 macrophage cells. Among the 181 kinds of plants, 99 species showed potent antioxidative activities and 20 extracts showed inhibitory activity towards nitric oxide production by more than 70% at a concentration of $100{\mu}g/mL$. Therefore, these plants should be considered promising candidates for the treatment of inflammatory diseases accompanying overproduction of NO.

• 생약학회지
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• 제38권2호통권149호
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• pp.170-175
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• 2007

Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.

• Natural Product Sciences
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• 제18권3호
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• pp.183-189
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• 2012

It is well known that inflammation is associated with neurodegenerative disorders, including Alzheimer' disease, Parkinson's disease and ischemia. Nitric oxide (NO), a pro-inflammatory mediator, is produced by inducible NO synthase (iNOS) in microglia as well as macrophages and appears to account for neurodegeneration. In this study, we aimed to isolate NO inhibitors from Pyrus pyrifolia by activity guided purification. As a result, we identified daucosterol and ${\beta}$-sitosterol, which have not been isolated from this plant before. This article also describes NO inhibitory activities of the methanol extract of Pyrus pyrifolia fruit and the isolated compounds from this, which are lupeol, betulin, betulinic acid, ${\beta}$-sitosterol and daucosterol, in LPS-activated RAW 264.7 and BV2 cell lines. Western blot analysis was performed to clarify the underlying mechanism of NO inhibition in the two cell lines.

• 한국응용과학기술학회지
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• 제36권4호
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• pp.1434-1442
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• 2019

본 연구는 홍삼추출물의 화장품소재로서의 항염증 효과의 가능성을 확인하는 것을 목적으로 한다. 본 연구에서는 홍삼 추출물을 사용하여 항염증에 대한 생물학적 활성평가를 수행하였다. 대식세포인 RAW 264.7 세포에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 홍삼 추출물의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. LPS로 유도된 RAW 264.7 세포 내에서 시료의 nitric oxide 저해활성은 25 ㎍/ml에서 71.2 %의 우수한 효능을 나타내었으며, western blot 시험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것으로 확인하였다. 이러한 실험 결과를 바탕으로 홍삼추출물이 항염 효과를 가진 화장품 소재로서의 가치를 제안할 수 있다.

• 대한화장품학회지
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• 제44권3호
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• pp.335-341
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• 2018

본 연구는 버드나무 속 식물의 화장품 소재로써의 활용 가능성을 평가하기 위해 수행하였다. 버드나무 속 식물 중 갯버들, 능수버들 및 키버들 70% 에탄올 추출물의 DPPH radical 소거능 분석을 통한 항산화 활성을 평가한 결과 모든 추출물이 대조군에 비해 DPPH radical 소거능이 유의적으로 증가하였다. 또한 3종의 버드나무 속 식물 추출물 처리는 RAW 264.7 세포의 nitric oxide (NO) 생성을 유의적으로 억제하여 항염 활성이 있는 것으로 확인되었으며, 모두 콜라게나제 저해 활성이 있는 것으로 확인되었다. 그 중 갯버들 추출물이 가장 높은 콜라게나제 저해활성을 나타내어 갯버들 추출물의 용매 분획물에 대한 추가적인 콜라게나제 저해활성을 분석한 결과, 물 및 부탄올 분획물이 가장 높은 콜라게나제 저해활성이 있는 것으로 확인되었다. 결과적으로 버드나무 속 식물 중 갯버들은 높은 콜라게나제 저해활성이 있는 것으로 확인되어 향후 효과적인 기능성 화장품 소재로 활용 가능 할 것으로 사료된다.

• Journal of Applied Biological Chemistry
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• 제53권2호
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• pp.91-98
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• 2010

본 연구에서는 결명자, 오미자, 구기자, 박하, 국화, 산마, 생강, 둥글레, 감초, 오가피, 녹차, 다시마를 발효시킨 들빛 발효액으로 여러 가지 생리활성에 대하여 분석하였다. 우선, 들빛 발효액의 플라보노이드 함량과 DPPH 활성을 측정하였다. 그 결과 들빛 발효액의 총 플라보이드 함량은 $3.4{\pm}0.5\;mg/g$으로, DPPH법을 통해 측정한 들빛 발효액의 radical 소거능은 96%로 나타났다. 들빛 발효액의 SOD 유사활성은 29%로 나타내었고, XOase에 의해 생성된 superoxide radical 소거능은 88%로 높게 나타났다. 들빛 발효액의 항고혈압 측정실험에서는 66%의 저해률을 나타내어 ACE 저해 활성이 뛰어난 것으로 나타났다. 혈전분해능에 대한 들빛 발효액의 분석 결과는 혈전용해제로 알려져 있는 plasmin과 유사한 활성을 보이고 있다. 들빛 발효액과 면역활성과의 연관성은 NO 생성 증가율과 LPS에 의해 유도되는 NO 생성 억제율 분석으로 조사되었다. 그 결과, 들빛 발효액은 무려 15배의 높은 NO 생성 증가율을 보였다. 또한 LPS에 의해 유도된 NO 생성 억제율은 들빛 발효액 100%에서 42%로 나타나 높은 면역조절능을 나타내었다. 이상의 결과는 들빛 발효액의 우수한 생리활성을 증명하고 있고, 항산화력, 항고혈압 효과, 혈전분해능 및 면역 조절활성이 높은 것으로 나타나 기능성 음료의 소재로서 그 활용도가 높을 것으로 판단된다.

• 약학회지
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• 제59권1호
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• pp.12-16
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• 2015

Several 8-cycloalkoxychrysin analogs were synthesized from 6-benzyloxy-5,8-dihydroxyflavone and cycloalkanols in 2 steps and we evaluated their inhibitory activities against NO production from LPS-induced RAW 264.7 cells. Among tested analogs, two analogs with cyclopentyl substructure (796 and 798) showed strong inhibitory activity against NO production.

• 생약학회지
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• 제30권4호
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• pp.413-416
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• 1999

During the screening for inhibitors on nitric oxide (NO) production in LPS-activated murine macrophage, RAW 264.7 cells, two coumarins were isolated from chloroform extract of Ponciri Fructus. They were identified as imperatorin (10), phellopterin (11) on the basis of spectroscopic methods. The $IC_{50}$ values for NO formatiom were about $5.4\;{\um}M$ and $35.0\;{\um}M$, respectively, and then eleven coumarins were tested for the inhibitory effects on NO production in activated macrophages. All the test coumarins inhibited NO production in concentration-dependent manner and furanocoumarins (6-11) showed much more potent inhibitory effect than simple coumarins. Among the compounds examined xanthotoxin (8) was the most potent inhibitors of NO production $(IC_{50}=1.4\;{\mu}M)$. Analysis of the structure-activity relationship among these coumarins led to the conclusion that the substitution of C-5 position in furanocoumarins reduces greatly their inhibitory potency although the substitution of C-8 does not almost affect it.