• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.842-851
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• 1998

Development and growth of Acartia steueri from Ilkwang Bay, southeastern coast of Korea, were determined under various temperatures and food condition (Isochrysis galbana and Dunaliella salina) in the laboratory. Relationship between egg hatching time ($D_E$, day) and temperature (T, $^{\circ}C$) was $D_E=744(T+3.5)^{-1.97}$. Mean hatching success was $88.4\%$ in temperature range of $8.4\~26.2^{\circ}C$. This suggests that A. steueri may be adapted to the temperature ranges in temperate regions. Post-embryonic development pattern was equiproportional rather than isochronal, with longer stage duration of copepodites than that of the nauplii. Stage duration of NI was the shortest of all developmental stages, while the duration of NII was the longest in duration of the other nauplii. Male was morphologically distinguished from female in CIV stage, and male was developed faster than female. Median development time at a given temperature was calculated from the Belehradek equation by proper multiplication of proportional constant for embryonic development. Body carbon weight at $19.1^{\circ}C$ was increased exponentially with time. Mean specific growth rate of nauplii (0.200 $d^{-1}$), except for NI stage, was not significantly different from that of copepodites (0.190 $d^{-1}$), with the lowest rate in NVI stage (0.107 $d^{-1}$), probably due to energy consumption for metamorphosis rather than somatic growth. The results suggest that although the development pattern determined in this study was not identical with Uye's result for A. steueri (e.g. Uye, 1980b), median development time may be applicable to calculate the stage duration of A. steueri in this study area.

• 전기의세계
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• v.23 no.4
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• pp.46-52
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• 1974

Generally, it has been regarded that there are two kinds of the effect of the electrodes, especially of the cathode in the gas discharge, (a) the effect caused by the difference of the cathode meterial and (b) the effect by the change of the cathode surface state even in the same meterials. Thus the two effects must be investigated independently to study the roles of the cathode in gas discharges. This paper measured sparking voltage in Rare gas (Ar, He) for the change of sparking voltage in repeating sparks and for the effect of (a) and (b) mentioned above, under the condition that the desorption of impurities from the cathod can be nigligible, and it is obtained that the correlative relations of the work function, sparking voltage and secondary coefficient are comparatively simple. In addition, the interesting character of the minimum point of the paschen's curves is found. The results were as follows; 1) The value of (pd)min with minimum pint of sparking voltage, (Vs)min, is 0.7-0.9 Torr. cm in Argon, but is 5.6-7.1 Torr. cm in Helium, and Paschen's curve in Helium shows slow curve than in Argon. 2) The minimum point of the Paschen's curve is satisfied actually Townsend's self sustaining criterion in Argon, but non-satisfaction in Helium, and the Townsend's secondary coefficient .gamma. action have compound property (.gamma.$_{i}$, .gamma.$_{p}$, .gamma.$_{m}$) in Helium. 3) The dependenting character of work function in Helium is less than in Argon. 4) The minimum point of sparking voltage increase under oxidized electrode than clear electrode in Au and Ag, but minimum point decrease in Ni and Cu.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7523-7527
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• 2013

Thymidylate synthase (TS) catalyzes the transfer of a methyl group from methylenetetrahydrofolate to dUMP to form dTMP. It is a primary target in the chemotherapy of colorectal cancers and some other neoplasms. In order to obtain pure protein for analysis of structure and biological function, an expression vector TS-pET28b (+) was constructed by inserting wild-type human thymidylate synthase (hTS) cDNA into pET28b (+). Then an expression strain was selected after transformation of the recombined plasmid into Rosetta (DE3). Fusion protein with His-tag was efficiently expressed in the form of inclusion bodies after IPTG induction and the content was approximately 40.0% of total bacteria proteins after optimizing expression conditions. When inclusion bodies were washed, dissolved and purified by Ni-NTA under denatured conditions, the purity was up to 90%. On SDS-PAGE and West-blotting, the protein band was found to match well with the predicted relative molecular mass-36kDa. Bioactivity was 0.1 U/mg. The results indicated that high-level expression of wild-type hTS cDNA can be achieved in prokaryotes with our novel method, facilitating research into related chemotherapy.

• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.18-25
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• 2015

To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. The average α-L-rhamnosidase activities on the p-nitrophenyl-α-L-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. To investigate the enzymatic properties, α-L-rhamnosidase-producing bacteria were isolated from the specimens, and the α-L-rhamnosidase gene was cloned from a selected organism, Bifidobacterium dentium, and expressed in E. coli. The cloned α-L-rhamnosidase gene contained a 2,673 bp sequcence encoding 890 amino acid residues. The cloned gene was expressed using the pET 26b(+) vector in E. coli BL21, and the expressed enzyme was purified using Ni²⁺-NTA and Q-HP column chromatography. The specific activity of the purified α-L-rhamnosidase was 23.3 µmol/min/mg. Of the tested natural product constituents, the cloned α-L-rhamnosidase hydrolyzed rutin most potently, followed by poncirin, naringin, and ginsenoside Re. However, it was unable to hydrolyze quercitrin. This is the first report describing the cloning, expression, and characterization of α-L-rhamnosidase, a flavonoid rhamnoglycosidemetabolizing enzyme, from bifidobacteria. Based on these findings, the α-L-rhamnosidase of intestinal bacteria such as B. dentium seem to be more effective in hydrolyzing (1 →6) bonds than (1 →2) bonds of rhamnoglycosides, and may play an important role in the metabolism and pharmacological effect of rhamnoglycosides.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.4
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• pp.425-435
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• 2016

This paper describes a spread-spectrum clock generation method by utilizing a ${\Delta}{\Sigma}$ digital PLL (DPLL) which is solely based on binary phase detection and does not require a linear time-to-digital converter (TDC) or other linear digital-to-time converter (DTC) circuitry. A 1-bit high-order ${\Delta}{\Sigma}$ modulator and a hybrid finite-impulse response (FIR) filter are employed to mitigate the phase-folding problem caused by the nonlinearity of the bang-bang phase detector (BBPD). The ${\Delta}{\Sigma}$ DPLL employs a two-point modulation technique to further enhance linearity at the turning point of a triangular modulation profile. We also show that the two-point modulation is useful for the BBPLL to improve the spread-spectrum performance by suppressing the frequency deviation at the input of the BBPD, thus reducing the peak phase deviation. Based on the proposed architecture, a 3.2 GHz spread-spectrum clock generator (SSCG) is implemented in 65 nm CMOS. Experimental results show that the proposed SSCG achieves peak power reductions of 18.5 dB and 11 dB with 10 kHz and 100 kHz resolution bandwidths respectively, consuming 6.34 mW from a 1 V supply.

• Nuclear Engineering and Technology
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• v.54 no.3
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• pp.959-964
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• 2022

Lead is an important material, both for fusion or fission reactors. The cross sections of natural lead should be validated because lead is a main component of lithium-lead modules suggested for fusion power plants and it directly affects the crucial variable, tritium breeding ratio. The presented study discusses a validation of the lead transport libraries by dint of the activation of carefully selected activation samples. The high emission standard ²⁵²Cf neutron source was used as a neutron source for the presented validation experiment. In the irradiation setup, the samples were placed behind 5 and 10 cm of the lead material. Samples were measured using a gamma spectrometry to infer the reaction rate and compared with MCNP6 calculations using ENDF/B-VIII.0 lead cross sections. The experiment used validated IRDFF-II dosimetric reactions to validate lead cross sections, namely ¹⁹⁷Au(n, 2n)¹⁹⁶Au, ⁵⁸Ni(n,p)⁵⁸Co, ⁹³Nb(n, 2n)^92mNb, ¹¹⁵In(n,n')^115mIn, ¹¹⁵In(n,γ)^116mIn, ¹⁹⁷Au(n,γ)¹⁹⁸Au and ⁶³Cu(n,γ)⁶⁴Cu reactions. The threshold reactions agree reasonably with calculations; however, the experimental data suggests a higher thermal neutron flux behind lead bricks. The paper also suggests ²⁵²Cf isotropic source as a valuable tool for validation of some cross-sections important for fusion applications, i.e. reactions on structural materials, e.g. Cu, Pb, etc.

• Nuclear Engineering and Technology
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• v.55 no.12
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• pp.4671-4677
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• 2023

For the first time Pb, Ni, and Cu nanocomposites were synthesized by versatile solution combustion synthesis using Aloevera extract as a reducing agent, to study the potential applications in X-ray/gamma, neutron, and Bremsstrahlung shielding. The synthesized Lead-Nickel-Copper (LNC) nanocomposites were characterized by PXRD, SEM, UV-VIS, and FTIR for the confirmation of successful synthesis. PXRD analysis confirmed the formation of multiphase LNC NCs and the Scherrer equation and the W-H plot gave the average crystal sizes of 19 nm and 17 nm. Surface morphology using SEM and EDX confirmed the presence of LNC NCs. Strong absorption peaks were analyzed by UV visible spectroscopy and the direct energy gap is found to be 3.083 eV. Functional groups present in the LNC NCs were analyzed by FTIR spectroscopy. X-ray/gamma radiation shielding properties were measured using NaI(Tl) detector coupled with MCA. It is found to be very close to Pb. Neutron shielding parameters were compared with traditional shielding materials and found LNC NCs are better than lead and concrete. Secondary radiation shielding known as Bremsstrahlung shielding characteristics also studied and found that LNC NCs are best in secondary radiation shielding. Hence LNC NCs find shielding applications in ionizing radiation such as X-ray/gamma and neutron radiation.

• Design & Manufacturing
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• v.13 no.2
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• pp.54-59
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• 2019

CNC(Computer Numerical Control) machine tools are being used in various industrial fields such as aircraft and automobiles. The machining conditions used in the mold industry are used, and the simulation and the experiment are compared. The tool used in the experiment was carried out to increase the reliability of the simulation of the cutting machining. The program used in the 3D-FEM (finite element method) was the AdvantEdge and predicted by down-milling. The tool model is used 3D-FEM simulation by using the cutting force, temperature prediction. In this study, we carried out the verification of cutting force by using a 3-axis tool dynamometer (Kistler 9257B) system when machining the plastic mold Steel machining of NAK-80. The cutting force experiment data using on the charge amplifier (5070A) is amplified, and the 3-axis cutting force data are saved as a TDMS file using the Lab-View based program using on NI-PXIe-1062Q. The machining condition 7 was the most similar to the simulation and the experimental results. The material properties of the NAK-80 material and the simulation trends reflected in the reverse design of the tool were derived similarly to the experimental results.

• Food Science of Animal Resources
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• v.24 no.1
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• pp.44-49
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• 2004

This study was conducted to evaluate the effects of non-specific immuno-stimulator(NIS) supplementation on growth performance and carcass characteristics in finishing pigs. A total of eighty[Duroc${\times}$Yorkshir${\times}$Landrace] pigs(60.55${\pm}$3.72 kg in average initial body weight) were used for a 9-week assay. Dietary treatments included as follows 1) CON(basal diet), 2) NIS1(basal diet for 6 weeks and 0.05% NIS for 3 weeks), 3) NIS2(basal diet for 3 weeks and 0.05% NIS for 6 weeks) and 4) NIS3(0.05% NIS for 9 weeks). Average daily gain during the feeding period was not significantly different among the treatments (p>0.05). However, average daily feed intake was higher in NIS2 than CON(p<0.05). Backfat thickness did not differ among the treatments(p>0.05). The appearance rate of A or B carcass grade was much higher in NIS1 (58%) and NIS3 (75%) than CON(50%) and NIS2(50%). L＊(lightness) value of loin was higher in NIS1 and NIS3 than CON and NIS2. However, there was no effect by dietary NIS(p>0.05). Although a＊(redness) value of loin was lower in CON than other treatments, there was no significant difference among the treatments(p>0.05). No remarkable differences were found in sensory properties(marbling, firmness and color) among the treatments. The results from the present study suggest that NIS could be an effective feed additive to improve pork quality. However, further research is needed to investigate effect of carcass characteristics.

• Korean Journal of Materials Research
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• v.7 no.3
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• pp.218-223
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• 1997

At the aim of finding a Fehased amorphous alloy with a wide supercooled liquid region (${\Delta}T_{x}=T_{x}-T_{g}$) before crystallization, the changes in glass transition temperatudfI$T_{g}$ and crystallization temperature ($T_{x}$) by the dissolution of additional M elements were examined for the $Fe_{80}P_{10}C_{6}B_{4}$(x~6at%. M= transition metals) amorphous alloys. The ${\Delta}T_{x}$ value is 27K for the Fe,,,P,,,C,,R, alloy and increases to 40K for the addition of M=4at%Hf, 4at%Ta or 4at%Mo. The increase in ${\Delta}T_{x}$ is due to the increase of $T_{x}$ exceeding the degree in the increase in $T_{g}$. The $T_{g}$ and $T_{x}$ increase with decreasing electron concentration (e/a) from about 7 38 to 7.05. The decrease of e/a also implies the increase in the attractive bonding state between the M elements and other constitutent elements. It is therefore said that $T_{g}$ and $T_{x}$ increase kith increasing attractive bonding force.