• Asian-Australasian Journal of Animal Sciences
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• v.31 no.5
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• pp.748-754
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• 2018

Objective: The preference evaluation of cattle is an important factor for estimation and improvement of the grazing amounts of newly introduced or bred grasses or cultivars in barn. This study was performed to assess the grazing behavior (the amount of grazing and/or the grazing speed) of cattle as indirect method using newly bred Brachiaria ruziziensis tetraploid strain 'OKI-1'(BR) hay as treatment group and Cloris gayana 'Callide' (CG) hay as control group. It also compared the feasibility of using behavioral differences between two groups as one criteria for evaluating preference by Japanese black cattle in barn. Methods: Three experiments were carried out using 12 growing Japanese Black cattle including 6 males and 6 females. In each experiment, the four Japanese Black cattle (2 males and 2 females) were placed in separated stall and allowed to graze BR and CG in manger that was separated into two portions for about 30 min. The position and behavior of the cattle were recorded, and weighed the residual of each gay at 15 and 30 minutes after experiment start. Results: The BR was superior to CG in chemical composition such as protein, fibers and non-fibrous carbohydrate. The cattle, over all, tended to prefer BR over CG in the first half 15 minutes in terms of the time spent and amount of grazing. Additionally, growing cattle exhibited neophilia for BR bred newly. Conclusion: These findings indicated the current approach could be applied for one of criteria to evaluate the preference of hay by Japanese black cattle under indoor housing environment.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1223-1232
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• 2017

Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating-type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of C. militaris were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of C. militaris. After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with MAT1-1 and the others with MAT1-2. Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4-bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new C. militaris strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing C. militaris strains.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.77-83
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• 2006

This study was carried out to investigate the agronomical and physicochemical properties of five newly bred sugary lines in YARI (Yeongnam Agricultural Research Institute) that developed from the cross of sugary and Hwayeongbyeo, Hwasambyeo and Seomjinbyeo. Culmlength of newly bred sugary lines were longer than that of Nampyeongbyeo as $84.2{\sim}99.3cm$. However the weight of 1,000 grains and yield were lower than that of Nampyeongbyeo by two to three times. According to physicochemical properties, most of the new bred sugary lines showed low contents of amylose and mineral compare to normal varieties. And all sugary lines showed much higher amount of the sucrose and glucose contents compare to Nampyeongbyeo. Especially the sucrose content of newly bred sugary line, 55413, was 5 times higher than that of Nampyeongbyeo nevertheless, fructooligosaccharide and isomaltooligsac charide were not detected. The amylopectin constitution of sugary lines showed more chairs of DP (Degree of Polymerization)<12 but fewer chains of DP $13{\sim}24$ compare to Nampyeongbyeo. Newly bred sugary lines were lower viscosity in RVA (Rapid Viscosity Analysis).

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.104-105
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• 2003

In order to breed high yielding good quality leaves mulberry cultivar, which is essential for silkworm rearing, the characteristics of newly bred mulberry strain were surveyed. Materials and Methods: materials -Mulberry Jamgsang 123 which is registered as newly bred mulbery cultivar Suilppong -Sikworm Baegogjam nd Daesungjam were reared for practical leaves quality examination in spring and autumn, respectively by feeding Jamsang 123 leaves (omitted)

• KOREAN JOURNAL OF CROP SCIENCE
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• v.21 no.1
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• pp.9-14
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• 1976

This experiment was conducted to find out the ., effects of various fertilizer level and Drill-seeding on the growth and yield of the two newly bred naked-barley varieties, Kwangsung and $Bangsa^6$ the former that was bred for the first time in Korea in 1972, is resistant to winter and productive, and the latter that was also bred in Korea by radioisotope treatment in 1974, is of short-stem, early maturing and productive. The grain yield in Drill-seeding was 8 percent higher than that in Conventional seeding. $Bangsa^6$ was more resistant to lodging and more adaptable to the amounts of the increased-fertilizer level than Kwangsung. In Drill-seeding, $Bangsa^6$ is thought to be more suitable naked-barley variety than Kwangsung.

• Korean Journal of Food Science and Technology
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• v.9 no.2
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• pp.144-152
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• 1977

The physiochemical properties of eight different cultivars or newly bred lines of glutionous rice were investigated and obtained following results; (1) The gelatinizing temperature, blue value and alkali number of starch separated from the sample cultivars or lines were similar to those of starch from conventional cultivar Olchal. The expansive power of three newly bred lines were somewhat weaker than that of starch from conventional cultivar, but the expansive power of other four lines were similar to that of conventional cultivar. (2) pH of cooked rice of the ten sample cultivars or breeding lines showed no discernible differences rangeing from 6.54 to 6.60. (3) The degree of gelatinization of cooked rice of newly bred lines were rather lower than that of conventional ones, but the degree of their retrogradation were somewhat higher than that of conventional cultivars. (4) In order to improve the palatability of cooked rice of Tongil (common rice), glutinous rice were mixed in different rate. The results showed that a mix ratio of six per cent glutinous rice was most favourable. The acceptability of common Tongil rice was improved when it was cooked with four to six per cent of glutinous rice mixed, and it showed no significant difference from that of Akibare alone cooked rice. Also no difference was noticed among newly bred glutinous lines in the acceptability when they cooked with common Tongil rice mixed. (5) Injolmi, Yaksik, Misitgaru, Twipap and Yugwa were prepared from glutinous rice of sample cultivars and or breeding lines to study their characteristics in processing and their acceptability. The results indicated that the acceptability score of newly bred lines were lower than that of conventional cultivar in cases of Twipap and Yugwa, but in cases of Injolmi, Yaksik and Misitgaru, identical score was obtained from both of newly bred lines and conventional cultivars.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.4
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• pp.408-417
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• 2019

This study was conducted to identify taxonomic differences in the characteristics of Rhodeus uyekii and Rhodeus ocellatus during their initial life history via an interspecific hybridization experiment. Hybrids were compared to their parent species, and the findings were used to inform developmental research in commercially useful aquarium fish. The hatching rates of the cross-bred eggs were 75.9% for cross UO (R. uyekii ♀${\times}$R. ocellatus♂) and 71.9% for cross OU (R. ocellatus♀${\times}$R. uyekii♂), which did not differ greatly from the hatching rates of the normal cross-bred group. Backcross experiments resulted in 100% egg mortality during development. Newly hatched larvae of the original hybrid crosses were similar to those of the maternal line, and the color of the egg yolk was similar to that of the paternal line; therefore, the respective traits of the interspecific parents were identifiable within the cross-bred offspring.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.55-62
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• 2018

We bred a mulberry cultivar named 'Saealchan' through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four local places (Jeonju, Gongju, Jangseong, and Sangju) for five years from 2013. This variety 'Saealchan' belongs to Morus alba L. selected from seedlings collected from mother mulberry 'Shimheung'. Saealchan was resistant to popcorn disease and high yielding variety in fruit productivity by 194% compared to control cultivar 'Shimheung (Morus alba L.)' for three years. Although fruits of 'Saealchan' was smaller in size and lower in sugar content of mulberry fruits compared to control variety 'Shimheung', it showed strong resistance to sclerotial disease compared to 'Shimheung'.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.123-133
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• 1992

This study was conducted to clarify progressed changes of plant types and the effects of the physiological and ecological components on improving ideotype of winter wheat. 12 wheat varieties were planted at the experimental farm of Wheat and Barley Research Institute in Suwon in 1990. As results of intensive wheat breeding for early maturity since 1959, heading, flowering and maturing dates have been shortened by 17, 15 and 14 dagys, respectively. The shortened days from sowing to heading and from heading to flowering contributed to the early maturity to improved. Physiological factors associated with heading time of wheat could be reprsented by growth habit, photoperiod responses, earliness in narrow sense and winter hardiness. For improving an early maturity of winter wheat, it would desired to maintain some degree of winter habit(III-IV), and recombination of more insensitivity to short day length and more shortened earliness in narrow sense than that of Saemil and Chugoku 81, and higher degree of winter hardiness. For improving the early maturity the more effective way must be of shortened days from sowing to heading, and days from flowering to maturity than days from heading to flowering. Ideotype of wheat will be desired to recombine two semi-dwarf genes with erect plant type being about 70-80cm, less stem elongation by late spring, long spike and many grains per spikelet. Average spike weight ratio was about 45-49% in high-yielding varieties, stem fresh weight was lighter, but spike fresh weight was heavier in new one while leaf fresh weight was similar to each other during the maturing periods. Average spike dry weight ratio was higher about 40～48%, and stem and leaf blade dry weights were lower in the newly bred varieties. Stem dry weight was heavier than spike or leaf dry weight in the old varieties of Yungkwang, Jangwang and Jinkwang. Leaf area index for the varieties showed normal distribution curve as the maximum point in booting stage. The maximum point of this curve come in early maturing wheat, and late in old one. The maximum points of LAI were 6.4～6.8 in the high-yielding varieties. Totals of LAI in each period investigated of old one were higher than those of newly bred being 24.6～28.8. Chlorophyll content of the high-yielding varieties of Chokwang, Geurumil and Saemil as higher than that of the old varieties Jangkwang, Jinkwang, Wonkwang and Sinkwang from regrowing period to April 21. after then slightly and even after heading. Net assimilation rate (NAR) was higher in high-yielding varieties with good plant type, and lower in old ones. Grain yield of the newly released varieties increased rapidly but slowly in the old ones. Change in water content of grain at the growing stage in newly bred was lower than that of the old bred. Diminishing rate of water content of grain in establishment per day was 1.2% average that of the old varieties including Yungkwang was 1.5%, and those of the newly bred including Chokwang were 0.9～1.1%. Chokwang, Naemil, and Saemil were the highest-yielding varieties of the Korean cultivars. Yields were increased by spikes per m$^2$, grain weight for the varieties bred in Suwon, and by spikes per m$^2$ for the varieties bred in Milyang.

• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.75-82
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• 2020

In this study, extracts from the Green ball apple peel (GBE) and the newly bred green ball apple from Korea showed inhibition effects on photo-aging factor regulation associated with skin aging. To investigate the inhibition effect on photo-aging factor regulation in skin, GBE was treated with UVB to induce photo-aging related factors in CCD986sk fibroblast cells. Photo-aging factor regulation effects showed that GBE inhibited UVB-stimulated matrix metalloproteinase (MMP)-1 and MMP-9 protein synthesis in collagen type I alpha 2 chain (COL1A2), MMP-1, MMP-9, and tissue inhibitors of metalloproteinase (TIMP)-1 protein expression. The expression of COL1A2 and TIMP-1 protein was significantly increased. The mRNA expression levels of COL1A2, MMP-1, MMP-9, hyaluronan synthase (HAS)2, transforming growth factor (TGF)-β, and TIMP-1 were decreased by GBE. The expression of TIMP-1 and TGF-β, which are regulators involved in matrix metalloproteinase and type I procollagen expression, was found to increase with increasing expression of COL1A2. The expression of HAS2, which is involved in the production of hyaluronic acid, one of the structural proteins constituting the skin, was also confirmed. Therefore, GBE showed excellent efficacy against photo-aging factor regulation and could be used as functional material to prevent and treat skin aging.