• Title/Summary/Keyword: New strain

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Production of a New Biosurfactant by a New Yeast Species Isolated from Prunus mume Sieb. et Zucc.

  • Jeong-Seon Kim;Miran Lee;Dae-Won Ki;Soon-Wo Kwon;Young-Joon Ko;Jong-Shik Kim;Bong-Sik Yun;Soo-Jin Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.8
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    • pp.1023-1029
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    • 2023
  • Biosurfactants reduce surface and interfacial tension due to their amphiphilic properties and are an eco-friendly alternative for chemical surfactants. In this study, a new yeast strain JAF-11 that produces a biosurfactant was selected using drop collapse method, and the properties of the extracts were investigated. The nucleotide sequences of the strain were compared with closely related strains and identified based on the D1/D2 domain of the large subunit ribosomal DNA (LSU) and internal transcribed spacer (ITS) regions. Neodothiora populina CPC 39399T, the closest species with strain JAF-11, showed a sequence similarity of 97.75% for LSU and 94.27% for ITS, respectively. The result suggests that the strain JAF-11 represents a distinct species that cannot be assigned to any existing genus or species in the family Dothideaceae. Strain JAF-11 produced a biosurfactant reducing the surface tension of water from 72 mN/m to 34.5 mN/m on the sixth day of culture and the result of measuring the critical micelle concentration (CMC) by extracting the crude biosurfactant was found to be 24 mg/l. The molecular weight 502 of the purified biosurfactant was confirmed by measuring the fast atom bombardment mass spectrum. The chemical structure was analyzed by measuring 1H nuclear magnetic resonance (NMR), 13C NMR, and two-dimensional NMRs of the compound. The molecular formula was C26H46O9, and it was composed of one octanoyl group and two hexanoyl groups to myo-inositol moiety. The new biosurfactant is the first report of a compound produced by a new yeast strain, JAF-11.

A Constitutive Model for Polymer-Bonded Explosive Simulants Considering Stress Softening and Residual Strain (응력연화와 잔류변형을 고려한 복합화약 시뮬런트의 구성방정식연구)

  • Yeom, KeeSun;Huh, Hoon;Park, Jungsu
    • Journal of the Korea Institute of Military Science and Technology
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    • v.17 no.6
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    • pp.844-852
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    • 2014
  • PBX simulant is known to exhibit highly nonlinear behaviors of deformation such as the stress softening, hysteresis under cyclic loading, residual strain after unloading, and aging. This paper proposes a new pseudo-elastic model for PBX simulant considering stress softening and residual strain. Uniaxial loading and unloading tests at quasi-static states were carried out in order to obtain the mechanical properties of the PBX simulants. And then the Dorfmann-Ogden model is modified to make it consistent with the test result of PBX simulants. Prediction with the new model shows a good correspondence to the experimental data demonstrating that the model properly describes stress softening and residual strain of PBX simulants.

Fracture Simulation of Low-Temperature High-Strength Steel (EH36) using User-Subroutine of Commercial Finite Element Code (상용 유한요소코드 사용자-서브루틴을 이용한 저온용 고장력강 (EH36)의 파단 시뮬레이션)

  • Choung, Joonmo;Nam, Woongshik;Kim, Younghun
    • Journal of Ocean Engineering and Technology
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    • v.28 no.1
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    • pp.34-46
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    • 2014
  • This paper discusses a new formulation for the failure strain in the average stress triaxiaility domain for a low-temperature high-strength steel (EH36). The new formula available at a low average stress triaxiality zone is proposed based on the comparison of two results from tensile tests of flat type specimens and their numerical simulations. In order to confirm the validity of the failure strain formulation, a user-subroutine was developed using Abaqus/Explicit, which is known to be one of the most popular commercial finite element analysis codes. Numerical fracture simulations with the user-subroutine were conducted for all the tensile tests. A comparison of the engineering stress-strain curves and engineering failure strain obtained from the numerical simulation with the user-subroutine for the tensile tests revealed that the newly developed user-subroutine effectively predicts the initiation of failure.

Taxonobic Characteristics of Strain Producing MR-387A and B,New Inhibitors of Aminopeptidase M,and their Production (신규의 Aminopeptidase M 저해제 MR-387A와 B를 생산하는 균주의 동정 및 저해제의 생산)

  • Chung, Myung-Chul;Chun, Hyo-Kon;Lee, Ho-Jae;Kho, Yung-Hee
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.447-452
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    • 1994
  • The strain SL-387 which produces new inhibitors of aminopeptidase M, MR-387A and B, was isolated from a soil sample. The strain has branched substrate mycelia, from which aerial hyphae develop in the form of open spirals. Spore surface is smooth. Melanoid and soluble pigme- nts were observed. The isolate contains LL-diaminopimelic acid in its cell wall hydrolysate, and has no pectinolytic activity. The strain SL-387 is closely related to Streptomyces griseoruber and S. naganishii, but is different from these strains in some cultural and physiological characteristics. This strain was, therefore, designated as Streptomyces sp. SL-387. The effects of several carbon and nitrogen sources on the production of the inhibitor were examined. Among them, glucose, galactose, mannose, and xylose were effective as a carbon source and soybean meal, soytone, fish meal, and gluten meal were effective as a nitrogen source. The maximum peak of the inhibitor production in jar fermentor was obtained on the fifth day of culture.

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A Study on the Lifted Flame Structure with Strain Rates in Premixed Impinging Jet Flames of Syngas (H2/CO) (합성가스(H2/CO) 예혼합 충돌 제트화염에서 신장률에 따른 부상된 화염 구조에 관한 연구)

  • SIM, KEUNSEON;JANG, BYOUNGLOK;LEE, KEEMAN
    • Journal of Hydrogen and New Energy
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    • v.26 no.4
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    • pp.347-356
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    • 2015
  • A study has been conducted numerically to investigate the lifted flat syngas flame structure of impinging jet flame configuration with the global strain rates in 10% hydrogen content. In this study, the effects of strain rate were major parameters on chemistry kinetics and flame structure at stagnation point. The numerical results were calculated by SPIN application of the CHEMKIN package. The strain rates were adjusted with Reynolds numbers of premixed syngas-air mixture. Different flame shapes were observed with different strain rates. As strain rate has increased, the flame temperature and axial velocity have been decreased due to the flame heat loss increment, and the OH radical reaction zones become narrower but each mole fractions are still constant. Also, the reversion of $H_2O$ product near stagnation point has been found out when strain rate has increased. This phenomenon is attributed to the rapid production of oxidizing radical reaction such as the R12 ($H+O_2(+M)=HO_2(+M)$), which makes the R18 ($HO_2+OH=O_2+H_2O$) reaction increment.

A Prediction Model for Low Cycle and High Cycle Fatigue Lives of Pre-strained Fe-18Mn TWIP Steel (Fe-18Mn TWIP강의 Pre-strain에 따른 저주기 및 고주기 피로 수명 예측 모델)

  • Kim, Y.W.;Lee, C.S.
    • Transactions of Materials Processing
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    • v.19 no.1
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    • pp.11-16
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    • 2010
  • The influence of pre-strain on low cycle fatigue behavior of Fe-18Mn-0.05Al-0.6C TWIP steel was studied by conducting axial strain-controlled tests. As-received plates were deformed by rolling with reduction ratios of 10 and 30%, respectively. A triangular waveform with a constant frequency of 1 Hz was employed for low cycle fatigue test at the total strain amplitudes in the range of ${\pm}0.4\;{\sim}\;{\pm}0.6$ pct. The results showed that low-cycle fatigue life was strongly dependent on the amount of pre-strain as well as the strain amplitude. Increasing the amount of prestrain, the number of reversals to failure was significantly decreased at high strain amplitudes, but the effect was negligible at low strain amplitudes. A new model for predicting fatigue life of pre-strained body has been suggested by adding ${\Delta}E_{pre-strain}$ to the energy-based fatigue damage parameter. Also, high-cycle fatigue lives predicted using the low-cycle fatigue data well agreed with the experimental ones.

A Study on the Single Side Strain Gauge Attatched Load Cell and Temperature Compensation (단일면에 스트레인게이지를 부착한 로드셀과 그 온도 보상에 관한 연구)

  • Park, Chan-Won;Choi, Gyu-Seok
    • Journal of Industrial Technology
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    • v.13
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    • pp.19-24
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    • 1993
  • Compensation of temperature is very important to make high precision load cell. In this study, we developed a new type load cell. The structure of the load cell has four strain gauges on single side of the load cell beam. Also a new temperature compensation method was proposed and these characteristics were better than previous one. This study will offer application to other type of load cell and another sensors.

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Inelastic buckling of tapered members with accumulated strain

  • Kim, M.C.;Lee, G.C.;Chang, K.C.
    • Structural Engineering and Mechanics
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    • v.3 no.6
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    • pp.611-622
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    • 1995
  • This paper is concerned with inelastic load carrying capacity of tapered steel members with or without accumulated plastic strains resulted from previous loading histories. A finite element program is developed using stiffness matrices of tapered members and is applicable for analyses with material and geometric nonlinearity. Results of analyses are compared with other available solutions and with experimental results.

A Study on the Yield Criterion of Metal Powders (금속 분말의 항복조건에 관한 연구)

  • 박성준;한흥남;오규환;이동녕
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1996.10a
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    • pp.131-138
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    • 1996
  • A new yield criterion for metal powder compaction based on continuum mechanics has been proposed. It includes three parameters to characterize the geometrical hardening of powder compact and strain hardening of incompressible metal matrix. The elasto-plastic finite element method to describe compaction of metal powders has been formulated using the new yield criterion. The values of parameters in the yield criterion can be determined using cold isostatic pressing(CIP). The finite element method can simulate compaction behavior of various copper powders.

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Shaking table study of a 2/5 scale steel frame with new viscoelastic dampers

  • Chang, K.C.;Tsai, M.H.;Lai, M.L.
    • Structural Engineering and Mechanics
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    • v.11 no.3
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    • pp.273-286
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    • 2001
  • Viscoelastic (VE) dampers have shown to be capable of providing structures with considerable additional damping to reduce the dynamic response of structures. However, the VE material appears to be sensitive to the variations in ambient temperature and vibration frequency. To minimize these effects, a new VE material has been developed. This new material shows less sensitivity to variations in vibration frequency and temperature. However, it is highly dependent on the shear strain. Experimental studies on the seismic behavior of a 2/5 scale five-story steel frame with these new VE dampers have been carried out. Test results show that the structural response can be effectively reduced due to the added stiffness and damping provided by the new type of VE dampers under both mild and strong earthquake ground motions. In addition, analytical studies have been carried out to describe the strain-dependent behavior of the VE damper. The dynamic properties and hysteresis behavior of the dampers can be simulated by a simple bilinear model based on the equivalent dissipated energy principle proposed in this study.