• Title/Summary/Keyword: New strain

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The Development of a New Soybean Strain Without Kunitz Trypsin Inhibitor, Lectin, and 7S α' Subunit Protein (쿠니츠트립인히비터, 렉틴 및 7S α' 서버유닛 3가지 단백질이 없는 콩 계통의 개발)

  • Chae, Won Gi;Choi, Sang Woo;Kang, Gyung Young;Chung, Jong Il
    • Journal of Life Science
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    • v.30 no.7
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    • pp.592-597
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    • 2020
  • Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'- subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI protein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F3 plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain's height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.

Novel dual-grating strain sensor signal processing technique using an unbalanced Mach-Zehnder interferometer (Mach-Zehnder 간섭계를 이용한 광섬유 격자쌍 스트레인 센서의 신호처리 방법)

  • 송민호;이병호;이상배;최상삼
    • Korean Journal of Optics and Photonics
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    • v.8 no.4
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    • pp.333-339
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    • 1997
  • We fabricated a sensor head which consists of spliced different-diameter fiber gratings for discrimination between strain and temperature. Because the fibers were drawn from the same preform, their temperature characteristics were the same but not for strain sensitivities which are inversely proportional to fibers cross-sectional areas. In measurement range of 0-1500$\mu$strain and 20-10$0^{\circ}C$, we could obtain, by using the matrix calculation, the unknown physical quantities within 10% of calculation error compared with the micrometer and thermocouple values. To improve the strain measurement accuracy, we suggest a new, novel method which deploys an unbalanced fiber Mach-Zehnder interferometer. This new signal processing technique converts the strain information to interference signal amplitude variation, temperature-independently. we obtained measurement accuracy nearly 80 times better than that obtainable with the conventional optical spectrum analyzer usage.

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An Experimental Study on the Diaphragm Deflection Characteristic of a Hydrogen Diaphragm Compressor (다이아프램식 수소압축기에서 다이아프램 변형특성에 관한 실험적 연구)

  • Shin, Young-Il;Park, Hyun-Woo;Lee, Young-Jun;Kim, Gyu-Bo;Song, Ju-Hun;Chang, Young-June;Jeon, Chung-Hwan
    • Journal of Hydrogen and New Energy
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    • v.20 no.4
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    • pp.274-282
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    • 2009
  • Diaphragm compressor is widely used for hydrogen compression because it achieves high gas pressure without gas contamination. Diaphragm deflecting in the cavity with high pressure formed by an oil compression is the most important component in the compressor. Therefore, it is necessary to obtain deflection degree of diaphragm to predict the damage point of diaphragm. The objective of this study is to estimate the diaphragm's damage point through diaphragm deflection test by implementing with strain gauges attached on several radial points. Without gas compression, strain sum of each points varied as similarly as the variation of the pressure with respect to time. And while the motor speed was slower than 400rpm, the strain near the rim was larger than that of the center. When motor speed, however, was over 500rpm, strain became similar to that of the center and the rim. With gas compression, it was shown that the variation of the strain sum was delayed against that of the pressure and the strain near the rim was much higher than that of the center.

Morphological and Phylogenetic Analyses Reveal a New Species of Genus Monochaetia Belonging to the Family Sporocadaceae in Korea

  • Seong-Keun Lim;Kallol Das;Soo-Min Hong;Sang Jae Suh;Seung-Yeol Lee;Hee-Young Jung
    • Mycobiology
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    • v.51 no.2
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    • pp.87-93
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    • 2023
  • The fungal strain belonging to the genus Monochaetia of the family Sporocadaceae was isolated from hairy long-horned toad beetle (Moechotypa diphysis) during the screening of microfungi associated with insects from Gangwon Province, Korea. The strain KNUF-6L2F produced white, light brown to dirty black surface, and olivaceous green colonies with the higher growth, while the closest strain M. ilicis KUMCC 15-0520T were light brown to brown, and M. schimae SAUCC 212201T light brown to brown toward center. The strain KNUF-6L2F produced shorter (5.7-14.0 ㎛) apical appendages than M. ilicis (6.0-24.0 ㎛), but similar to M. schimae (7.0-12.5 ㎛). Three median cells of KNUF-6L2F were light brown to olivaceous green, whereas brown and olivaceous cells were observed from M. ilicis and M. schimae, respectively. And the strain KNUF-6L2F produced larger conidiogenous cells than M. ilicis and M. schimae. Additionally, phylogenetic analyses based on molecular datasets of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and β-tubulin (TUB2) genes corroborated the strain's originality. Thus, the strain is different from other known Monochaetia species, according to molecular phylogeny and morophology, hence we suggested the new species Monochaetia mediana sp. nov. and provided a descriptive illustration.

Identification and characterization of Dunaliella salina OH214 strain newly isolated from a saltpan in Korea

  • Minjae, Kim;Hyeon Jun, Oh;Khanh, Nguyen;EonSeon, Jin
    • ALGAE
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    • v.37 no.4
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    • pp.317-329
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    • 2022
  • Carotenoids are effective antioxidants that are found in various photosynthetic organisms. Marine microalgae are an advantageous bioresource for carotenoid production because they do not compete with other crops for freshwater and arable land. This study reports a newly isolated Dunaliella strain from the Geumhong Saltpan on Yeongjong Island, West Sea, Korea. The new strain was isolated and classified as Dunaliella salina through phylogenetic analysis and was named the OH214 strain (Deposit ID: KCTC14434BP). The newly isolated strain can survive in a wide range of NaCl concentrations (0.3-5.0 M NaCl), but grows well in 0.6 to 1.5 M NaCl culture medium. Under high-light conditions (500 ± 10 μmol photons m-2 s-1), the cells accumulated three times more β-carotene than under low-light conditions (50 ± 5 μmol photons m-2 s-1). The cells accumulated 2.5-fold more β-carotene under nitrogen-deficient (1 mM KNO3) conditions (3.24 ± 0.36 ㎍ 106 cells-1) than in nitrogen-sufficient conditions (>5 mM KNO3). The lutein content under nitrogen-deficient conditions (1.73 ± 0.09 ㎍ 106 cells-1) was more than 24% higher than that under nitrogen-sufficient conditions. Under the optimized culture condition for carotenoid induction using natural seawater, D. salina OH214 strain produced 7.97 ± 0.09 mg g DCW-1 of β-carotene and 4.65 ± 0.18 mg g DCW-1 of lutein, respectively. We propose that this new microalga is a promising strain for the simultaneous production of β-carotene and lutein.

Right Ventricular Strain Is Associated With Increased Length of Stay After Tetralogy of Fallot Repair

  • Ranjini Srinivasan;Jennifer A. Faerber;Grace DeCost;Xuemei Zhang;Michael DiLorenzo;Elizabeth Goldmuntz;Mark Fogel;Laura Mercer-Rosa
    • Journal of Cardiovascular Imaging
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    • v.30 no.1
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    • pp.50-58
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    • 2022
  • BACKGROUND: Little is known regarding right ventricular (RV) remodeling immediately after Tetralogy of Fallot (TOF) repair. We sought to describe myocardial deformation by cardiac magnetic resonance imaging (CMR) after TOF repair and investigate associations between these parameters and early post-operative outcomes. METHODS: Fifteen infants underwent CMR without sedation as part of a prospective pilot study after undergoing complete TOF repair, prior to hospital discharge. RV deformation (strain) was measured using tissue tracking, in addition to RV ejection fraction (EF), volumes, and pulmonary regurgitant fraction. Pearson correlation coefficients were used to determine associations between both strain and CMR measures/clinical outcomes. RESULTS: Most patients were male (11/15, 73%), with median age at TOF repair 53 days (interquartile range, 13,131). Most patients had pulmonary stenosis (vs. atresia) (11/15, 73%) and 7 (47%) received a transannular patch as part of their repair. RV function was overall preserved with mean RV EF of 62% (standard deviation [SD], 9.8). Peak radial and longitudinal strain were overall diminished (mean ± SD, 33.80 ± 18.30% and -15.50 ± 6.40%, respectively). Longer hospital length of stay after TOF repair was associated with worse RV peak radial ventricular strain (correlation coefficient (r), -0.54; p = 0.04). Greater pulmonary regurgitant fraction was associated with shorter time to peak radial RV strain (r = -0.55, p = 0.03). CONCLUSIONS: In this small study, our findings suggest presence of early decrease in RV strain after TOF repair and its association with hospital stay when changes in EF and RV size are not yet apparent.

Paleostress of the Joseon and Pyeongan Supergroups in South Korea using the New Calcite Strain Gauge (NCSG)

  • Jang, Bo-An;Ko, Chin-Surk;Kim, Jung-Han;Kim, Cheong-Bin;Kang, Seong-Seung
    • The Journal of Engineering Geology
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    • v.24 no.3
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    • pp.311-322
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    • 2014
  • Limestone bodies under the tectonic environment have experienced various tectonic processes, and also changed the stress state. In this study, calcite twins found in limestones of the Joseon Supergroup and Pyeongan Supergroup in the northeastern part of the Ogcheon Belt, South Korea were measured, then the paleostress (i.e., the maximum shortening axis) was reconstructed using the new calcite strain gauge (NCSG) technique. The average twin thickness and average twin intensity increase as the total twin strain increases. We utilize the appearance of twins, the average twin thickness and average twin intensity, and the total twin strain to estimate that the observed calcite twins were produced at temperatures of < $200^{\circ}C$ in the Joseon Supergroup and $170^{\circ}C$ in the Pyeongan Supergroup. In the Joseon Supergroup, the dominant direction of the maximum shortening axis WNW-ESE to NW-SE; NE-SW shortening is also observed. The maximum shortening axes in the Pyeongan Supergroup are oriented NW-SE and NE-SW. The NE-SW direction of maximum shortening is associated with the occurrence of the Songrim orogeny of the Paleozoic to Early Jurassic, and the NW-SE direction of maximum shortening correlates to the Daebo orogeny of the Early Jurassic to Late Jurassic. It is thus concluded that the paleostress across the study area changed from NE-SW to NW-SE during the Mesozoic.

Characterization of a new commercial strain 'Guseol' by intra-specific hyphal anastomosis in Pleurotus ostreatus (계통간 교잡에 의한 느타리 품종 '구슬'의 육성 및 그 특성)

  • Yoo, Young-Bok;Kim, Eun-Jung;Kong, Won-Sik;Jang, Kab-Yeul;Shin, Pyung-Gyun
    • Journal of Mushroom
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    • v.10 no.3
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    • pp.109-114
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    • 2012
  • To develop new variety of oyster mushroom, 63 intra-specific hybrids between the strain Suhan and #Nongi201 were developed using hyphal anastomosis technique in 2004. The Po2008-275 hybrid between the dikaryon strain 04-154(Suhan x #Nongi201) and the monokaryon strain derived from ASI2487 were developed using hyphal anastomosis in 2008. The Po2008-275 was shown the best cultural characteristics, selected to be a new variety and named as 'Guseol'. The new commercial strain, 'Guseol' had dark grey pilei and grows well under spring and autumn conditions in Korea. The fruiting bodies of 'Guseol' were of an excellent quality in that not only the stipe was thick and long but also the pileus was small and hard. The optimum temperatures for mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $10{\sim}16^{\circ}C$, respectively. Time period required for the initiation of the first fruiting body was about 3 to 5 days depending on the temperatures. The shape of fruiting body was thin funnel shape. Fruiting body production per box($43{\times}43{\times}12cm$) was about $1545{\pm}400.9g$ which was almost 137% quantity compared to that of parental strain 04-154. Relatively low temperature incubation ($11^{\circ}C$) resulted in the development of better quality of 'Guseol' mushrooms. When two different media including potato dextrose medium and mushroom complete medium were compared, the mycelial growth of this mushroom were much faster in mushroom complete medium. Similar results were observed with other variety '#Chunchu2'. Analysis of the genetic characteristics of the new commercial strain 'Guseol' showed a major DNA profile as that of the parental 04-154 when primer URP 1, primer URP 2 and primer URP 5 were used, but different to '#Chunchu2' that was used as a control. This new variety of the dark grey oyster mushroom had smart and high quality image that corresponds well to "health food". We therefore expect that this new strain will satisfy the consumers demand for variety and excellent mushrooms.

An efficient four node degenerated shell element based on the assumed covariant strain

  • Choi, Chang-Koon;Paik, Jong-Gyun
    • Structural Engineering and Mechanics
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    • v.2 no.1
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    • pp.17-34
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    • 1994
  • This paper proposes a new four node degenerated shell element. In the formulation of the new element, the assumed covariant shear strains are used to avoid the shear locking problem, and the assumed covariant membrane strains are applied to alleviate the membrane locking problem and also to improve the membrane bending performance. The assumed covariant strains are obtained from the covariant strain field defined with respect to the element natural coordinate system. This formulation enables us to obtain a shell element, which does not produce spurious singular modes, avoids locking phenomena, and excels in calculation efficiency. Several examples in this paper indicate that, despite its simplicity, the achieved accuracy and convergence are satisfactory.

Studies on antibiotics against Wheat black rust (I) (밀의 항흑수병 항생물질의 연구 1)

  • 정영기
    • Korean Journal of Microbiology
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    • v.19 no.3
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    • pp.108-114
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    • 1981
  • In order to isolate microorganisms which produce antibiotics aganist wheat black rust, some bacteria, molds, and actinomycetes were isolated from soils and screened for the production of antibiptics against wheat black rust. Beacuse wheat black rust-puccinia graminis--is a complete parsitic mold which can't grow in artifical medium, new method for the screening of antibiotic producing microorgsnisms against wheat black rust developed by using live leaves of wheat. With new method, a strain No. $480HS_{20}$ which produces a substnace having strong and Puccinia graminis activity and very narrow antimicrobial spectrum was isolated. the substance produced by the strain No.$480HS_{20}$ had better anti Puccinia graminis activity than any other known antifungal antibotics such as kasurgamycin, balasticidins, actidione, antimycin, ologomycin. And the substance was observed to be very stable at heat and ultraviolet light. The strain was indentified as Bacillus subtilis.

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