• Title/Summary/Keyword: Neurofilaments

Search Result 18, Processing Time 0.021 seconds

Effects of Gamijingansikpungtang on Cultured Spinal Motor Neurons (가미진간식풍탕(加味鎭肝熄風湯)이 손상(損傷)된 배양척수운동신경세포(培養脊髓運動神經細胞)에 미치는 영향(影響))

  • Kim, Seong-Hwan;Sim, Jeong-Sub;Kim, Kang-San;Kang, Byung-Ki;Lee, Jae-Ik
    • The Journal of Internal Korean Medicine
    • /
    • v.21 no.2
    • /
    • pp.283-290
    • /
    • 2000
  • The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine(XO/HX) and the effects of herbal extracts such as Jingansikpungtang(JST) and Gamijingansikpungtang(GJST) on the treatment of the toxic effects. For this purpose, experiments with the cultured nerve cells from the spinal motor neurons of new born mice were done. The results of these experiments were as follows. XO/HX, a oxygen radical-generating system, decreased the survival rate of the cultured cells on NR assay. MTT assay, the amount of neurofilaments and increased the amount of total proteinand increased the lipid peroxidation and the amount of LDH JST has the efficacy of increasing the amount of neurofilaments and total protein, and decreasing the lipid peroxidation and the amount of LDH, GJST has efficacy of increasing the amount of neurofilaments and total protein, and decreasing lipid peroxidation and the amount of LDH. From the above results, it is concluded that JST and GJST have marked efficacy as a treatment for the damages caused in the XO/HX mediated oxidative stress. And JST and GJST are thought to have certain pharmacologicall effects. Further clinical study of this pharmacological effects of JST and GJST should be complemented.

  • PDF

Effects of Chilbokyeumgamibang(七福飮加味方) on the Cerebral Cortex Neuron injured by Glucose Oxidase (칠복음가미방(七福飮加味方)이 Glucose Oxidase에 의해 손상(損傷)된 대뇌피질(大腦皮質) 신경세포(神經細胞)에 미치는 영향(影響))

  • Choi Kong-Han;Gang Hyeong-Won;Lyu Yeoung-Su
    • Journal of Oriental Neuropsychiatry
    • /
    • v.10 no.1
    • /
    • pp.53-78
    • /
    • 1999
  • As the average life span have been lengthened and the rate of senile population have been raised, chronic degenerative diseases incident to aging has been increased rapidly and become a social problem. With this social background, recently, the facts that oxygen radicals(OR) have toxic effects on Central Nervous System and Peripheral Nervous System and cause neuropathy such as Parkinson's Disease, Alzheimer Disease have been turned out, and accordingly lots of studies on the mechanism of the toxic effects of OR on nerves, the diseases caused by OR and the approaches to curing the diseases have been made. The purpose of this study is to examine the toxic effects caused by Glucose Oxidase(GO) and the effects of herbal extracts such as Chilbokyeum(CBY), Chilbokyeumga Acori Rhizoma(CAR), Acori Rhizoma(AR) on the treatment of the toxic effects. For this purpose, experiments with the cultured cell from the cerebrums of new born mice were done. The results of these experiments were as follows. 1. GO, a oxygen radical, decreased the survival rate of the cultured cells on NR assay, MTT assay and amount of neurofilaments and increased the amount of total protein, lipid peroxidation and the amount of LDH. 2. CBY have efficacy of increasing the amount of neurofilaments and total protein and decreasing lipid peroxidation and the amount of LDH. 3. CAR have efficacy of increasing the amount of neurofilaments and total protein and decreasing lipid peroxidation and the amount of LDH. 4. AR have efficacy of increasing the amount of neurofilaments and total protein. From the above results, It is concluded that Chilbokyeumgamibang has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process. And Chilbokyeumgamibang is thought to have certain pharmacological effects on controlling over aging and treating Dementia. Further clinical study of this pharmacological effects of Chilbokyeumgamibang should be complemented.

  • PDF

Effects of Cheongsimyeonja-tang water extract on the Cultured Primary Hippocampal Cell Damaged by XO/HX (청심연자탕(淸心蓮子湯) 수추출물(水抽出物)이 XO/HX에 의해 손상(損傷)된 배양(培養) 해마신경세포(海馬神經細胞)에 미치는 영향(影響))

  • Lee, Jae-Heung;Kim, Hyong-Soon;Bae, Young-Chun;Kim, Kyung-Yo;Won, Kyoung-Sock;Hwang, Seung-Yeon
    • Journal of Sasang Constitutional Medicine
    • /
    • v.14 no.3
    • /
    • pp.132-145
    • /
    • 2002
  • The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine(XO/HX) and the effects of herbal extracts such as Cheongsimyeonjatang(CYT) on the treatment of the toxic effects. For this purpose, experiments with the cultured hippocampal cells from new born mice were done. The results of these experiments were as follows. 1. XO/HX, a oxygen radical-generating system, decreased the survival rates of the cultured cells on MTT assay and NR assay, protein synthesis, and amounts of neurofilaments. 2. CYT have the efficacy of increasing protein synthesis decreased by XO/HX. 3. CYT have the efficacy. of increasing the amount of neurofilaments decreased by XO/HX. From the above results, it is suggested that Cheongsimyeonjatang (CYT) have marked efficacy as a protection for the damages caused by the XO/HX-mediated oxidative stress.

  • PDF

Effects of Jogihaeatag(調氣解瘀湯) on the Cerebral Cortex Neuron injured by XO/XA (조기해어탕(調氣解瘀湯)이 XO/XA에 의해 손상(損傷)된 대뇌피질(大腦皮質) 신경세포(神經細胞)에 미치는 영향(影響))

  • Lee Yong-Keun;Kang Hyung-Won;Lyu, Yeoung-Su
    • Journal of Oriental Neuropsychiatry
    • /
    • v.10 no.2
    • /
    • pp.29-45
    • /
    • 1999
  • As the average life span has been lengthened and the rate of senile population has been raised, chronic degenerative diseases incident to aging have been increased rapidly and become a social problem. With this social background, recently, oxygen radicals(OR) have toxic effects on Central Nervous System and Peripheral Nervous System and cause neuropathy such as Parkinson's Disease, Alzheimer Disease. The purpose of this study is to examine the toxic effects caused by Xanthine Oxidase(XO) and the effects of herbal extracts such as Jokihaeatang(JHT) on the treatment of the toxic effects. For this purpose, experiments with the cultured cell from the cerebrums of new born mice were done. The results of these experiments were as follows. 1. X0, an oxygen radical, decreased the survival rate of the cultured cells on NR assay, MTT assay and amount of neurofilaments and increased the amount of lipid peroxidation. 2. JHT have efficacy of increasing the amount of neurofilaments.

  • PDF

The Effects of Jihwangyeumja and GamiJihwangyeumja water extract on The Cultured Primary Hippocampal Cell Damaged by XO/HX (지황음자와 가미지황음자 유출액이 XO/HX로 손상된 배양 해마신경세포에 미치는 효과)

  • Lee Yong Geun;Kim Sang Ho;Min Sang Jun;Yang Hee Suk;Jang Hyun Ho;Kim Tae Hean;Kang Hyung Won;Lyu Yeoung Su
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.16 no.5
    • /
    • pp.989-1000
    • /
    • 2002
  • The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine(XO/HX) and the effects of herbal extracts such as JHYJ and GJHYJ on the treatment of the toxic effects. For this purpose, experiments with the cultured hippocampal cells from new born mice were done. The results of these experiments were as follows. 1. XO/HX, a oxygen radical-generating system, decreased the survival rates of the cultured cells on XTT assay and INT assay, the amount of DNA syntheses, and the amount of neurofilaments, and increased the lipid peroxidation. 2. JHYJ and GJHYJ have the efficacy of increasing the survival rates of the cultured cells. 3. JHYJ and GJHYJ have the efficacy of increasing the amount of neurofilaments and of decreasing the lipid peroxidation. 4. JHYJ and GJHYJ have the efficacy of increasing the amount of DNA syntheses. From the above results, it is suggested that Jihwangyeumja and Gamijihwangyeumja have marked efficacy as a treatment for the damages caused by the XO/HX-mediated oxidative stress. And Jihwangyeumja and Gamijihwangyeumja are thought to have certain pharmacological effects. Further dinical study of this pharmacological effects of Jihwangyeumja and Gamijihwangyeumja should be complemented.

Evaluation of the Various Artificial Skin Substitutes Implanted onto Nude Mice (누드마우스를 이용한 다양한 피부 대체물의 성능비교)

  • Lee, Won Jai;Lee, Dong Won;Hur, Jae Young;Lee, Young Dae;Park, Beyoung Yun;Rah, Dong Kyun
    • Archives of Plastic Surgery
    • /
    • v.35 no.2
    • /
    • pp.127-133
    • /
    • 2008
  • Purpose: The purpose of this study is to evaluate the remodeling process of the various skin substitutes in 4th and 6th weeks following the transplantation when transplanted onto nude mice. Methods: Three types of artificial skin substitutes, such as PLGA scaffold with keratinocyte sheets(group 1), acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), bioengineered skin($Neoderm^{(TM)}$)(group 3), were applied to the wound on nude mice. All mice were killed in 2, 4 weeks and/or 6 weeks after grafting and tissue samples were harvested from the back of mice. The changes in wound size, degree of angiogenesis, formation of basement membrane and epidermis, density of collagen fibers and neural restoration were examined. Results: There was no significant changes in wound size among the three groups. However, the size of wound decreased in the non-substituted group due to contracture. Degree of angiogenesis and systhesis of collagen or neurofilaments were mostly increased in bioengineered skin($Neoderm^{(TM)}$)(group 3), followed by acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), PLGA scaffold with keratinocyte sheets (group 1). However, group 3 and group 2 showed similar thickness of basement membrane and epidermis. Conclusion: We found that degree of angiogenesis, formation of basement membrane and skin appendages, density of collagen fibers and neurofilaments can be the categories to evaluate the success of artificial skin substitution in early stages.

Effects of Mangeum-tang and Gamimangeum-tang on the Cultured Spinal Dorsal Root Ganglion Cells (만금탕 및 가미만금탕이 배양 척수후근신경절 세포에 미치는 영향)

  • Choi Gyu Seon;Yun Sang Hak;Yeom Seung Ryong;Lee Su kyung;Shin Byung Cheul;Kwon Young Dal;Song Yung Sun
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.17 no.2
    • /
    • pp.503-509
    • /
    • 2003
  • The purpose of this study is to examine the toxic effects caused by xanthine oxidase/hypoxanthine (XO/HX) and the effects of herbal extracts such as Mangeum-tang (萬金湯: MGT) and Gamimangeum-tang (加味萬金湯: GMGT) on the treatment of the toxic effects. The results of these experiments were XO/HX, an oxygen radical-generating system, decreased the survival rates of the cultured cells on XTT assay, the amount of DNA syntheses, and the amount of neurofilaments, and increased c-fos positive cells, MGT and GMGT have the efficacy of increasing the survival rates of the cultured cells by increasing the amount of neurofilaments and DNA synthesis and decreasing the c-fos positive cells damaged by XO/HX, From the above results, it is suggested that MGT and GMGT have marked efficacy as a treatment for the damages caused by the XO/HX-mediated oxidative stress. And MGT and GMGT are thought to have certain pharmacological effects.

The effect of rod domain A148V mutation of neurofilament light chain on filament formation

  • Lee, In-Bum;Kim, Sung-Kuk;Chung, Sang-Hee;Kim, Ho;Kwon, Taeg-Kyu;Min, Do-Sik;Chang, Jong-Soo
    • BMB Reports
    • /
    • v.41 no.12
    • /
    • pp.868-874
    • /
    • 2008
  • Neurofilaments (NFs) are neuronal intermediate filaments composed of light (NF-L), middle (NF-M), and heavy (NF-H) subunits. NF-L self-assembles into a "core" filament with which NF-M or NF-H co-assembles to form the neuronal intermediate filament. Recent reports show that point mutations of the NF-L gene result in Charcot-Marie-Tooth disease (CMT). However, the most recently described rod domain mutant of human NF-L (A148V) has not been characterized in cellular level. We cloned human NF-L and used it to engineer the A148V. In phenotypic analysis using SW13 cells, A148V mutation completely abolished filament formation despite of presence of NF-M. Moreover, A148V mutation reduced the levels of in vitro self-assembly using GST-NF-L (H/R) fusion protein whereas control (A296T) mutant did not affect the filament formation. These results suggest that alanine at position 148 is essentially required for NF-L self-assembly leading to subsequent filament formation in neuronal cells.

Species Dependence of Neurofilament Structures: Monte Carlo Simulation studies of Residue-Based Neurofilament Models

  • Kim, Seon-Ok
    • Proceeding of EDISON Challenge
    • /
    • 2014.03a
    • /
    • pp.225-235
    • /
    • 2014
  • 6종의 Intermediate filament 중 type IV인 Neurofilaments (NFs)는 신경세포에 존재하는 세포골격세사로 heavy NF(NF-H), medium NF(NF-M), light NF(NF-L) 세가지의 분자 질량 단백질로 구성되어 있다. NF의 side arm은 interfilament spacing과 axonal caliber를 조절하는 중요한 역할을 한다고 생각되어왔다. 또한 이에 대해서 각각의 protein의 역할은 알아내기 위해 isolated NF의 형태와 구조에 대해 많은 연구가 이루어졌는데, NF의 구조적 특성은 NF sidearm의 tail 부분에서 phosphorylation의 정도에 따른 Lys-Ser-Pro(KSP) repeats의 charge distribution을 통해 알 수 있다. 지금까지 NF에 대한 많은 연구가 이루어졌지만 인간에 한해서만 진행되었다. 그렇기 때문에 본 연구에서는 주어진 amino acid sequence와 각 species의 NF-H:NF-M:NF-L의 비율의 정보를 이용하여 The constant-NVT ensemble MC simulation을 통해 인간뿐만이 아닌 다른 species에 대한 NF의 구조적 특성을 알아보고자 한다.

  • PDF

Electrobehavioral and Pathological Characteristics in Cerebral Cortical Dysplasia Induced by External Irradiation in the Rat (방사선조사에 의해 피질이형성증 백서의 전기행동학적, 병리조직학적 특징)

  • Choi, Ha-Young
    • Journal of Korean Neurosurgical Society
    • /
    • v.29 no.7
    • /
    • pp.861-867
    • /
    • 2000
  • Purpose : Neuronal migration disorder(NMD) is a major underlying pathology of patients with intractable epilepsy. The role of NMD on seizure susceptibility or epileptogenecity, however, has not been documented. Methods : External irradiation of total amount of 250 cGY was performed to the fetal rats on days 16(E16) and 17(E17) of gestation. After delivery, the rats of 230-260g were decapitated for the histopathologic study. Epileptog-enecity of the NMD was studied by observing electroclinical events after intraperitoneal kainic acid(KA) injection in the control rats and NMD rats. Results : Histopathologic findings revealed focal and/or diffuse cortical dysplasia consisting of dyslamination of the cerebral cortex and appearance of the cytomegalic neurons, neuronal heterotopia in the periventricular white matter, dispersion of the pyramidal layer and the dentate gyrus of the hippocampus, and agenesis of the corpus callosum. Abnormal expression of neurofilaments protein(NF-M/H) was characteristically observed in the dysplastic neurons of the neocortex and hippocampus. Early ictal onset and prolonged ictal activity on EEG and clinical seizures were observed from the NMD rats unlike with the control rats. Conclusions : Exteranl irradiation on the fetal rats produced NMD. And the rats with NMD were highly susceptible to kainic acid provoked seizures. This animal model would be useful to study the pathophysiology of clinically relevant NMDs.

  • PDF