• 한국수산과학회지
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• 제56권1호
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• pp.124-132
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• 2023

This study aimed to cross between Korean and Japanese pure lines of Neopyropia strains to establish cross breeding technology and identify a superior variety that harbors the strength of both parents. Four crossing combinations were tried using three methods, resulting in 1,476 single conchocelis colonies. The three co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) markers (EF-1α/Mse I, TOP2/Mse I, car A/ApaL I) were used to distinguish heterozygotic sporophytes and their maternal lines obtained from the inter and intraspecific cross-fertilization within the wild type of Neopyropia strains. Of the 1,476 colonies, 26.9% (218) were heterozygotes obtained from the nuclear CAPS markers. Their maternal line was clearly confirmed using organelle CAPS marker and chimeric thallus was obtained from crossing experiment of Japanese N. yezoensis (♀) and Korean N. yezoensis (♂). The use of CAPS markers improved the efficiency of crossbreeding by quickly screening heterozygotes and maternal lines in the conchocelis phase, which otherwise required pigmentation mutants as genetic markers.

• ALGAE
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• 제38권4호
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• pp.283-294
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• 2023

Previous research indicated that free-living sporangial filament keep hollow morph under high-culture density and form bipartite cells under low-culture density, while the following conchospore release was inhibited by high light. Here, we further explored the molecular bases of these affects caused by light and culture density using a transcriptome analysis. Many differentially expressed genes (DEGs) related to carbon dioxide concentration and fixation, photosynthesis, chlorophyll synthesis and nitrogen absorption were upregulated under high-light conditions compared with low-light conditions, indicating the molecular basis of rapid vegetative growth under the former. The stress response- and ion transport-related DEGs, as well as the gene encoding the vacuole formation-brefeldin A-inhibited guanine nucleotide exchange protein (BIG, py05721), were highly expressed under high-density conditions, indicating the molecular basis of the hollow morph of free-living sporangial filaments under high-culture density conditions. Additionally, the brefeldin A treatment indicated that the hollow morph was directly influenced by vacuole formation-related vesicle traffic. Others DEGs related to cell wall components, zinc-finger proteins, ASPO1527, cell cycle and cytoskeleton were highly expressed in the low density with low-light group, which might be related to the formation and release of conchospores. These results provide a deeper understanding of sporangial filaments in Neopyropia yezoensis and related species.

• 현장농수산연구지
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• 제25권3호
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• pp.19-29
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• 2023

본 연구는 실내배양을 통해 방사무늬김(N. yezoensis) 패각 사상체의 성장과 수온변화가 각포자 형성 및 방출에 미치는 영향을 조사하였다. 7주간의 실내배양을 통해서 유리 사상체가 패각에 잠입하여 성장하고 각포자가 형성되는 것을 관찰할 수 있었다. 또한 실내 배양기에서 패각 사상체 성장 및 각포자 형성이 충분히 가능하였고, 배양기간도 단축하는 결과를 보였다. 패각 내 각포자 형성량을 조사하기 위하여 배양온도를 10℃와 18℃, 28℃로 나누어 6주간 배양하였다. 10℃에서는 평균 1주 127개, 2주 127개, 3주 95개, 4주 90개, 5주 76개, 6주 75개가 관찰되었고, 18℃에서는 평균 1주 141개, 2주 135개, 3주 94개, 4주 153개, 5주 162개, 6주 2개가 관찰되었으며, 28℃에서는 1주 167개, 2주 102개, 3주 148개, 4주 157개, 5주 270개, 6주 138개가 관찰되었다. 각포자 평균 형성량은 28℃가 가장 많았고, 18℃, 10℃ 순이었다. 패각에서 방출된 각포자 수는 직접적으로 계수가 어려움에 따라 유엽의 개체수로 방출량을 확인하였다. 각포자 방출에 따른 유엽 개체수는 10℃에서 1주 0개, 2주 189개, 3주 200개, 4주 89개, 5주 56개, 6주 27개가 관찰되었고, 18℃에서는 1주 0개, 2주 26개, 3주 546개, 4주 16개, 5주 17개체, 6주 154개체가 관찰되었으며 28℃에서는 거의 관찰되지 않았다. 본 연구를 통하여 패각 속에서 형성된 각포자는 28℃에서 18℃ 조건으로 옮긴 뒤 3주 후 최대 방출하였고, 이어서 3주 후 2번째로 많이 방출하는 패턴을 확인하였다.